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The map label for this gene is btuB [H]

Identifier: 110835232

GI number: 110835232

Start: 2695118

End: 2696968

Strand: Reverse

Name: btuB [H]

Synonym: ABO_2371

Alternate gene names: 110835232

Gene position: 2696968-2695118 (Counterclockwise)

Preceding gene: 110835233

Following gene: 110835231

Centisome position: 86.44

GC content: 57.21

Gene sequence:

>1851_bases
ATGGTATTTCGTCCCATTGCAGCGTGTGCTGCGATGTCTTTGTGTGCGTGGCCCTTGCTTGCCTACGCGGAATCTTCACC
GCTTGATGCTGCTGTTCTGCGCGTTTCTATGCTCGATCCCGTGGTCGTCACGCCTACGCTGGCCAGCCGTACTACAGAGG
CGTCATTGGCCTCGGTAACGGTGCTCGATGAGAAGCAATTACGCGAGCGTCAGCCCCAGGATATTACCGAGATGCTAAGA
GGCCAGCCCGGCGTGGATATCACCGGTAATGGTGCCTTCGGTAAGGCCACCAGTGTTTATTTGCGGGGTACGGGCAGTAG
CTCAACGTTGTTAATGGTTGATGGTGTGCGCCTGCGTTCGGCAACCGGCGGCAGCCCGTCTTGGCAGTTCCTGCCTCCCA
CCTTGATTGACCGTATTGAAATTGTGCGTGGCCCCCGTGGCAGTTTGTATGGCGCGGATGCTGTGGGTGGCGTGGTGCAG
GTTTTTACACCTGGGGCTCGGGATCTCAGCAATGAACAAACCGGCTGGGTGGAGCTGGGAGCGGGTTCGTTTAATAGCCG
CCGGGTGGCAGCGGGCGCGCAGGGGGAGCAGGACGGTACGCGCTATAACTTGGCGGTGGACCGCTTTGATACCGATGGCA
CAGAAATTCGTGAAGGCAGTGATGATCGTGGCTACGACAACACATCGGCGGTGGCTCGGGTAACGCATCGCTTCGATAAC
GATGCGGAAATCGGCGTGTTCGGTTTTCGGGCAGCCGGCAGCACCGAGTTTGAAGGCAGCTCGCCTTCGCAGAAAAAAGT
GACCGACTACGCCATGCAGACTGCGGGTGTGAAAGGGCAGTTGTGGGTGACGGAACGTTGGTTGAGCAAACTTATTCTTA
GCGATTCCCGCGACGAGAACGAGAACTTCACCGACGGCAACCGAGACAGCATTTTTAACACCCGTAGCCGTGCGGCTAAC
TGGCAGAATGTGTTTGCTTTGGGGGATCACCAGCTAGTGGTGGGTGCCGATTACCTGCAAGACCTAGTGGACAGCACCAC
GCTGTATTTGGAAGACGAGCGTGACAATAGCGCCGGTTTTGCCCAGCTGTTATTAAACTTTGGTGCCTTCGATGTGCAGG
CCAGTGGCCGTTACGACGACAACGAAGCCTATGGCAGTGAAACCACTGGCGGACTGGCGTTGGGCTACAAGCTCGATAAC
CATCACCGGTTACGGGCCAGCTATGGCACCGCTTTCCGTGCGCCGACCTTTAATGATTTGTACTTCCCCGGCTTTGGTAA
TGACCAGCTGGACCCGGAAAGTTCCGCCAGCACCGAGCTCGGGGTGAGGGGCCAGTATCAGCGTTGGTACTGGGATATTG
CCGCCTACCAGACCGATGTGGATGACCTGATTGCCTATGCCTTGCAGGGTGGGCGTTATGCGCCGTTCAATGTGAACCGG
GCGCGCATTCGGGGTGTCGAACTGGCTACCGGTGTTGAGATAAACCAGTGGCGCGCTGGTGCGGCCTTTACGGCTCAGGA
TCCAGAAGATCGGGATACGGGGTTGGTGCTTCAGCGTCAGTCAAAGCGTAGCTTCCGTTTTGATCTGGACCGAGCCATGG
GGAACGTTTCCCTAGGAGGCAGTGTGGTTTTGCAAGGGCACCGTTATAACGATGCGCAAAACGACGTGCGGCTGTCTGGG
TTCGGTTTGCTGGATTTGCGCGTCGGCTGGAATTTCGCACGGAACTGGTCCACACGCCTGGCGGTGAAAAACGTGCTCGA
CAAGGAGTACGCCACGGCGCAGAACTTTGCTGGTTGGAACTATATGAACGCAGGGCGTTCGGTAATGTTGAGCCTGCGCT
ACGATAGGTAG

Upstream 100 bases:

>100_bases
GGTGGGGTGTGTTGGCGCGATTGCGCCTGTCTCCCTGTGCTTGTTCTGCTGCGGCTAAATTCAGGCCGTGCGGGTGGGCA
CGGCAGAGGGGGATCTCTCG

Downstream 100 bases:

>100_bases
AGCAAGAATAGCGCTGCGAGATGAGATGGAGTGAGTGAATGCGCGTTTTACGCTGGGGGTTGTTGCTGACGTTGTTGGCC
ATGTTTGATACGGCCATGGC

Product: TonB-dependent receptor protein

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 616; Mature: 616

Protein sequence:

>616_residues
MVFRPIAACAAMSLCAWPLLAYAESSPLDAAVLRVSMLDPVVVTPTLASRTTEASLASVTVLDEKQLRERQPQDITEMLR
GQPGVDITGNGAFGKATSVYLRGTGSSSTLLMVDGVRLRSATGGSPSWQFLPPTLIDRIEIVRGPRGSLYGADAVGGVVQ
VFTPGARDLSNEQTGWVELGAGSFNSRRVAAGAQGEQDGTRYNLAVDRFDTDGTEIREGSDDRGYDNTSAVARVTHRFDN
DAEIGVFGFRAAGSTEFEGSSPSQKKVTDYAMQTAGVKGQLWVTERWLSKLILSDSRDENENFTDGNRDSIFNTRSRAAN
WQNVFALGDHQLVVGADYLQDLVDSTTLYLEDERDNSAGFAQLLLNFGAFDVQASGRYDDNEAYGSETTGGLALGYKLDN
HHRLRASYGTAFRAPTFNDLYFPGFGNDQLDPESSASTELGVRGQYQRWYWDIAAYQTDVDDLIAYALQGGRYAPFNVNR
ARIRGVELATGVEINQWRAGAAFTAQDPEDRDTGLVLQRQSKRSFRFDLDRAMGNVSLGGSVVLQGHRYNDAQNDVRLSG
FGLLDLRVGWNFARNWSTRLAVKNVLDKEYATAQNFAGWNYMNAGRSVMLSLRYDR

Sequences:

>Translated_616_residues
MVFRPIAACAAMSLCAWPLLAYAESSPLDAAVLRVSMLDPVVVTPTLASRTTEASLASVTVLDEKQLRERQPQDITEMLR
GQPGVDITGNGAFGKATSVYLRGTGSSSTLLMVDGVRLRSATGGSPSWQFLPPTLIDRIEIVRGPRGSLYGADAVGGVVQ
VFTPGARDLSNEQTGWVELGAGSFNSRRVAAGAQGEQDGTRYNLAVDRFDTDGTEIREGSDDRGYDNTSAVARVTHRFDN
DAEIGVFGFRAAGSTEFEGSSPSQKKVTDYAMQTAGVKGQLWVTERWLSKLILSDSRDENENFTDGNRDSIFNTRSRAAN
WQNVFALGDHQLVVGADYLQDLVDSTTLYLEDERDNSAGFAQLLLNFGAFDVQASGRYDDNEAYGSETTGGLALGYKLDN
HHRLRASYGTAFRAPTFNDLYFPGFGNDQLDPESSASTELGVRGQYQRWYWDIAAYQTDVDDLIAYALQGGRYAPFNVNR
ARIRGVELATGVEINQWRAGAAFTAQDPEDRDTGLVLQRQSKRSFRFDLDRAMGNVSLGGSVVLQGHRYNDAQNDVRLSG
FGLLDLRVGWNFARNWSTRLAVKNVLDKEYATAQNFAGWNYMNAGRSVMLSLRYDR
>Mature_616_residues
MVFRPIAACAAMSLCAWPLLAYAESSPLDAAVLRVSMLDPVVVTPTLASRTTEASLASVTVLDEKQLRERQPQDITEMLR
GQPGVDITGNGAFGKATSVYLRGTGSSSTLLMVDGVRLRSATGGSPSWQFLPPTLIDRIEIVRGPRGSLYGADAVGGVVQ
VFTPGARDLSNEQTGWVELGAGSFNSRRVAAGAQGEQDGTRYNLAVDRFDTDGTEIREGSDDRGYDNTSAVARVTHRFDN
DAEIGVFGFRAAGSTEFEGSSPSQKKVTDYAMQTAGVKGQLWVTERWLSKLILSDSRDENENFTDGNRDSIFNTRSRAAN
WQNVFALGDHQLVVGADYLQDLVDSTTLYLEDERDNSAGFAQLLLNFGAFDVQASGRYDDNEAYGSETTGGLALGYKLDN
HHRLRASYGTAFRAPTFNDLYFPGFGNDQLDPESSASTELGVRGQYQRWYWDIAAYQTDVDDLIAYALQGGRYAPFNVNR
ARIRGVELATGVEINQWRAGAAFTAQDPEDRDTGLVLQRQSKRSFRFDLDRAMGNVSLGGSVVLQGHRYNDAQNDVRLSG
FGLLDLRVGWNFARNWSTRLAVKNVLDKEYATAQNFAGWNYMNAGRSVMLSLRYDR

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=637, Percent_Identity=28.414442700157, Blast_Score=213, Evalue=4e-56,
Organism=Escherichia coli, GI1788478, Length=165, Percent_Identity=33.3333333333333, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI1786798, Length=142, Percent_Identity=33.0985915492958, Blast_Score=76, Evalue=8e-15,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 67635; Mature: 67635

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFRPIAACAAMSLCAWPLLAYAESSPLDAAVLRVSMLDPVVVTPTLASRTTEASLASVT
CCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEECCCHHCCCCCCCEEEEE
VLDEKQLRERQPQDITEMLRGQPGVDITGNGAFGKATSVYLRGTGSSSTLLMVDGVRLRS
EECHHHHHHCCCHHHHHHHCCCCCCEEECCCCCCCEEEEEEEECCCCCEEEEEECEEEEC
ATGGSPSWQFLPPTLIDRIEIVRGPRGSLYGADAVGGVVQVFTPGARDLSNEQTGWVELG
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHCCEEEEECCCCHHCCCCCCCEEEEC
AGSFNSRRVAAGAQGEQDGTRYNLAVDRFDTDGTEIREGSDDRGYDNTSAVARVTHRFDN
CCCCCCCEEECCCCCCCCCCEEEEEEEEECCCCCHHCCCCCCCCCCCHHHHHHHHHHCCC
DAEIGVFGFRAAGSTEFEGSSPSQKKVTDYAMQTAGVKGQLWVTERWLSKLILSDSRDEN
CCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHCCCCCCC
ENFTDGNRDSIFNTRSRAANWQNVFALGDHQLVVGADYLQDLVDSTTLYLEDERDNSAGF
CCCCCCCCCCCCCCHHHCCCCCEEEEECCCEEEEEHHHHHHHHCCEEEEEECCCCCCHHH
AQLLLNFGAFDVQASGRYDDNEAYGSETTGGLALGYKLDNHHRLRASYGTAFRAPTFNDL
HHHHHHCCCEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCEEEEECCCCEECCCCCCCE
YFPGFGNDQLDPESSASTELGVRGQYQRWYWDIAAYQTDVDDLIAYALQGGRYAPFNVNR
ECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHCCHHHHHHHHHCCCEECCCCCCH
ARIRGVELATGVEINQWRAGAAFTAQDPEDRDTGLVLQRQSKRSFRFDLDRAMGNVSLGG
HHEEEEEEECCCEEEEECCCCEECCCCCCCCCCCEEEEECCCCCCEEEHHHHCCCEECCC
SVVLQGHRYNDAQNDVRLSGFGLLDLRVGWNFARNWSTRLAVKNVLDKEYATAQNFAGWN
EEEEECCCCCCCCCCEEEECCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
YMNAGRSVMLSLRYDR
CCCCCCEEEEEEECCC
>Mature Secondary Structure
MVFRPIAACAAMSLCAWPLLAYAESSPLDAAVLRVSMLDPVVVTPTLASRTTEASLASVT
CCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEECCCHHCCCCCCCEEEEE
VLDEKQLRERQPQDITEMLRGQPGVDITGNGAFGKATSVYLRGTGSSSTLLMVDGVRLRS
EECHHHHHHCCCHHHHHHHCCCCCCEEECCCCCCCEEEEEEEECCCCCEEEEEECEEEEC
ATGGSPSWQFLPPTLIDRIEIVRGPRGSLYGADAVGGVVQVFTPGARDLSNEQTGWVELG
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHCCEEEEECCCCHHCCCCCCCEEEEC
AGSFNSRRVAAGAQGEQDGTRYNLAVDRFDTDGTEIREGSDDRGYDNTSAVARVTHRFDN
CCCCCCCEEECCCCCCCCCCEEEEEEEEECCCCCHHCCCCCCCCCCCHHHHHHHHHHCCC
DAEIGVFGFRAAGSTEFEGSSPSQKKVTDYAMQTAGVKGQLWVTERWLSKLILSDSRDEN
CCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHCCCCCCC
ENFTDGNRDSIFNTRSRAANWQNVFALGDHQLVVGADYLQDLVDSTTLYLEDERDNSAGF
CCCCCCCCCCCCCCHHHCCCCCEEEEECCCEEEEEHHHHHHHHCCEEEEEECCCCCCHHH
AQLLLNFGAFDVQASGRYDDNEAYGSETTGGLALGYKLDNHHRLRASYGTAFRAPTFNDL
HHHHHHCCCEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCEEEEECCCCEECCCCCCCE
YFPGFGNDQLDPESSASTELGVRGQYQRWYWDIAAYQTDVDDLIAYALQGGRYAPFNVNR
ECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHCCHHHHHHHHHCCCEECCCCCCH
ARIRGVELATGVEINQWRAGAAFTAQDPEDRDTGLVLQRQSKRSFRFDLDRAMGNVSLGG
HHEEEEEEECCCEEEEECCCCEECCCCCCCCCCCEEEEECCCCCCEEEHHHHCCCEECCC
SVVLQGHRYNDAQNDVRLSGFGLLDLRVGWNFARNWSTRLAVKNVLDKEYATAQNFAGWN
EEEEECCCCCCCCCCEEEECCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
YMNAGRSVMLSLRYDR
CCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA