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The map label for this gene is rph

Identifier: 110636340

GI number: 110636340

Start: 4320930

End: 4321646

Strand: Reverse

Name: rph

Synonym: Meso_4016

Alternate gene names: 110636340

Gene position: 4321646-4320930 (Counterclockwise)

Preceding gene: 110636345

Following gene: 110636339

Centisome position: 97.94

GC content: 62.06

Gene sequence:

>717_bases
ATGCGACCATCAAGACGGCAACCGGACGAGATGCGCGCAATCAGCTTCGAGCGTGGTGTATCGAAACATGCCGAGGGCTC
CTGCCTCGTGAGGTTCGGTGACACCCACGTATTGTGCACGGCAAGCCTGGAGGAGAAGGTTCCCGCCTGGTTGCGGAACT
CCGGGAAGGGCTGGGTGACGGCCGAGTATGGCATGCTTCCACGCTCAACCGGAGAACGAATGCGCAGGGAGGCGGCGAGC
GGCAAGCAGGGCGGGCGCACGCTAGAGATCCAGCGCCTGGTCGGCCGCAGCCTGCGTTCAGTCATCGACCTTGAAGCACT
CGGAGAAATGCAGATCACCGTCGACTGCGACGTCCTGCAAGCCGATGGCGGCACGCGCACAGCGGCGATCACAGGCGGGT
TCGTGGCGCTGCACGACTGCCTAAGCTGGATGCAGGCGCGCCAAATGGTCACCGTAGAGCGCGTTTTGAAGGACCATGTC
GCGGCGATCTCCTGCGGAATATACGACGGCACGCCTGTGCTCGACCTCGACTACGCGGAGGATTCAGCAGCCGAAACCGA
CGCGAATTTCGTAATGACCGGCAAGGGCGGCATCGTGGAGATCCAGGGGACCGCCGAAGGCGCGCCTTTCACGGAAGAGG
AATTTCATGAACTTCTTCGCCTGGCCAGGAAAGGTATCTTCCGGCTGGTGGAGCTGCAGAAGATGGCGGTCTCGTAA

Upstream 100 bases:

>100_bases
TGTTGGCGGTCGTTCACGAACATGCTTTTCCTTTTGCGTCGGCAAGCCGTGGCAGATACAAGCCGGCCCAATACAAGCAA
ATGCGAGCGGGAGACCCGAC

Downstream 100 bases:

>100_bases
AACGGATGAAGCCTGGGGCCATACTCGAAGCGGCGCTCTATGTGAACGATCTTGCGGAAGCCGAGCGCTTCTATGGAGAG
GTGCTGGGCCTCGATCTTCT

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MRPSRRQPDEMRAISFERGVSKHAEGSCLVRFGDTHVLCTASLEEKVPAWLRNSGKGWVTAEYGMLPRSTGERMRREAAS
GKQGGRTLEIQRLVGRSLRSVIDLEALGEMQITVDCDVLQADGGTRTAAITGGFVALHDCLSWMQARQMVTVERVLKDHV
AAISCGIYDGTPVLDLDYAEDSAAETDANFVMTGKGGIVEIQGTAEGAPFTEEEFHELLRLARKGIFRLVELQKMAVS

Sequences:

>Translated_238_residues
MRPSRRQPDEMRAISFERGVSKHAEGSCLVRFGDTHVLCTASLEEKVPAWLRNSGKGWVTAEYGMLPRSTGERMRREAAS
GKQGGRTLEIQRLVGRSLRSVIDLEALGEMQITVDCDVLQADGGTRTAAITGGFVALHDCLSWMQARQMVTVERVLKDHV
AAISCGIYDGTPVLDLDYAEDSAAETDANFVMTGKGGIVEIQGTAEGAPFTEEEFHELLRLARKGIFRLVELQKMAVS
>Mature_238_residues
MRPSRRQPDEMRAISFERGVSKHAEGSCLVRFGDTHVLCTASLEEKVPAWLRNSGKGWVTAEYGMLPRSTGERMRREAAS
GKQGGRTLEIQRLVGRSLRSVIDLEALGEMQITVDCDVLQADGGTRTAAITGGFVALHDCLSWMQARQMVTVERVLKDHV
AAISCGIYDGTPVLDLDYAEDSAAETDANFVMTGKGGIVEIQGTAEGAPFTEEEFHELLRLARKGIFRLVELQKMAVS

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family

Homologues:

Organism=Escherichia coli, GI157672248, Length=211, Percent_Identity=61.1374407582938, Blast_Score=259, Evalue=1e-70,
Organism=Caenorhabditis elegans, GI71981632, Length=177, Percent_Identity=29.9435028248588, Blast_Score=78, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNPH_MESSB (Q11B42)

Other databases:

- EMBL:   CP000390
- RefSeq:   YP_676548.1
- ProteinModelPortal:   Q11B42
- SMR:   Q11B42
- STRING:   Q11B42
- GeneID:   4181163
- GenomeReviews:   CP000390_GR
- KEGG:   mes:Meso_4016
- NMPDR:   fig|266779.1.peg.3044
- eggNOG:   COG0689
- HOGENOM:   HBG737187
- OMA:   MLPRATG
- PhylomeDB:   Q11B42
- ProtClustDB:   PRK00173
- BioCyc:   MSP266779:MESO_4016-MONOMER
- HAMAP:   MF_00564
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336
- TIGRFAMs:   TIGR01966

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.56

Molecular weight: Translated: 26087; Mature: 26087

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPSRRQPDEMRAISFERGVSKHAEGSCLVRFGDTHVLCTASLEEKVPAWLRNSGKGWVT
CCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCEEEEECCHHHHCCHHHHCCCCCEEE
AEYGMLPRSTGERMRREAASGKQGGRTLEIQRLVGRSLRSVIDLEALGEMQITVDCDVLQ
EECCCCCCCHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEE
ADGGTRTAAITGGFVALHDCLSWMQARQMVTVERVLKDHVAAISCGIYDGTPVLDLDYAE
CCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCEEEEECCC
DSAAETDANFVMTGKGGIVEIQGTAEGAPFTEEEFHELLRLARKGIFRLVELQKMAVS
CCCCCCCCCEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRPSRRQPDEMRAISFERGVSKHAEGSCLVRFGDTHVLCTASLEEKVPAWLRNSGKGWVT
CCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCEEEEECCHHHHCCHHHHCCCCCEEE
AEYGMLPRSTGERMRREAASGKQGGRTLEIQRLVGRSLRSVIDLEALGEMQITVDCDVLQ
EECCCCCCCHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEE
ADGGTRTAAITGGFVALHDCLSWMQARQMVTVERVLKDHVAAISCGIYDGTPVLDLDYAE
CCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCEEEEECCC
DSAAETDANFVMTGKGGIVEIQGTAEGAPFTEEEFHELLRLARKGIFRLVELQKMAVS
CCCCCCCCCEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA