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The map label for this gene is cmlA [H]

Identifier: 110636268

GI number: 110636268

Start: 4247016

End: 4248221

Strand: Reverse

Name: cmlA [H]

Synonym: Meso_3944

Alternate gene names: 110636268

Gene position: 4248221-4247016 (Counterclockwise)

Preceding gene: 110636269

Following gene: 110636266

Centisome position: 96.28

GC content: 60.78

Gene sequence:

>1206_bases
ATGTCCAGAAATATCCGGCTGTGGACCTACAGCCTGCCTGTCGCATTGATGCTGCTGGCACCCTTCAACATCCTTGCCTC
GCTTGGCATGGACATTTACCTGCCTGCCATTCCCGCAATGCCAGGAATCCTCGGCACCACACCCAATGTGGTGCAACTAA
CGCTCACCCTCTACATGATTATGCTCGGGGTAGGACAGATCCTCTTCGGGCCTCTTTCGGACCGTCTCGGCCGCAGGCCC
GTCCTGATCGGCGGGGCGATCCTCTTCGCCACTGCTTCCTTTGCTCTTGCGGCCACATCCTCGGCCGGGCTGTTCCTGCT
TCTCCGGTTGGCTCAGGCCACGGGCGCGGCTGCGGTGCTGGTTGCCCTTTTCGCCACCATCCGCGACGTCTATGCCGAGC
GTCCAGAAAGCGCCGTCATATACAGCCTCATGAATGCCATGCTGGCATTCGTGCCTGCGGTCGGTCCCATCGCCGGCGCG
CTGATCATGGGGATCTGGGGATGGCGCGCCATCTTCCTTGCGCTGGGCATTCCGGCGCTCATCGTGCTCACCCTCATGCT
TCCCGCCTGGCACGAGACCCGGCAGGCGAAGGGAGGACAGGATAGCGCGGGTTTTAGGCCGGTGCTCAGAAGCCCAGCCT
TTTGGACCTATACCATCGGCTTCGGAACTGCCATGGGCACATTCTTCGTGTTCTTCTCTACCGCCCCTCGCGTTCTGATC
GATGGAGCAGGCTTTTCCGAGCTCGATTTCAGCTTCGCTTTTGCAAGCGTGGCCATCGCCATGATTATTACCACGCGGTT
CGCCAAGAGCTTCGTGGCGCGATGGGGAATTCCGGGCACCCTGATGCGCGGCATGGTGATGTTGATCCTGGGGGCGTTCC
TGCTCGCGATCGGCCAGTTGTTCCTCGCCCCGTCCTTTTGGTCCCTTGTCGTGCCCATGTGGCTGATTGCGATCGGCATC
GTCTTTACGACCGCCGTCACCGCTAATGGCGCATTGCAGGAGTTCGGAGAAACTGCGGGAATGGCGGTCGCCCTGCATTT
CTGTATCCAGAGCCTCCTTGTCGGCATTGTCGGCACGGGATTTGTGATCATGCTGGATGGCAGCACCGCATGGCCTCTGA
TCACCAACGCGGCCGGCATGGCGGCGGTCACTTTGATCGCGCTCTTTTGGCTCGGCGGCCGCAATTTGCATCCGCAAACC
GCGTGA

Upstream 100 bases:

>100_bases
ACGTGCTTTCGTTGTTGCGGCCGTCTGCCCGGTTCCAATACCTGTCACCTGACAGCGCAGACCGCCGCCGAACATCATTC
ATTCGAGCGGATGCTTTGTC

Downstream 100 bases:

>100_bases
TGCCAACGCCTGAGCGGTCCTGATCGCGGCCGCTCAGGCAGCCCCGCACATTGGCTGGAGGTCTCGTCTGACGATGCTTC
TGTTGCGGCTGCCAAGGTCG

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MSRNIRLWTYSLPVALMLLAPFNILASLGMDIYLPAIPAMPGILGTTPNVVQLTLTLYMIMLGVGQILFGPLSDRLGRRP
VLIGGAILFATASFALAATSSAGLFLLLRLAQATGAAAVLVALFATIRDVYAERPESAVIYSLMNAMLAFVPAVGPIAGA
LIMGIWGWRAIFLALGIPALIVLTLMLPAWHETRQAKGGQDSAGFRPVLRSPAFWTYTIGFGTAMGTFFVFFSTAPRVLI
DGAGFSELDFSFAFASVAIAMIITTRFAKSFVARWGIPGTLMRGMVMLILGAFLLAIGQLFLAPSFWSLVVPMWLIAIGI
VFTTAVTANGALQEFGETAGMAVALHFCIQSLLVGIVGTGFVIMLDGSTAWPLITNAAGMAAVTLIALFWLGGRNLHPQT
A

Sequences:

>Translated_401_residues
MSRNIRLWTYSLPVALMLLAPFNILASLGMDIYLPAIPAMPGILGTTPNVVQLTLTLYMIMLGVGQILFGPLSDRLGRRP
VLIGGAILFATASFALAATSSAGLFLLLRLAQATGAAAVLVALFATIRDVYAERPESAVIYSLMNAMLAFVPAVGPIAGA
LIMGIWGWRAIFLALGIPALIVLTLMLPAWHETRQAKGGQDSAGFRPVLRSPAFWTYTIGFGTAMGTFFVFFSTAPRVLI
DGAGFSELDFSFAFASVAIAMIITTRFAKSFVARWGIPGTLMRGMVMLILGAFLLAIGQLFLAPSFWSLVVPMWLIAIGI
VFTTAVTANGALQEFGETAGMAVALHFCIQSLLVGIVGTGFVIMLDGSTAWPLITNAAGMAAVTLIALFWLGGRNLHPQT
A
>Mature_400_residues
SRNIRLWTYSLPVALMLLAPFNILASLGMDIYLPAIPAMPGILGTTPNVVQLTLTLYMIMLGVGQILFGPLSDRLGRRPV
LIGGAILFATASFALAATSSAGLFLLLRLAQATGAAAVLVALFATIRDVYAERPESAVIYSLMNAMLAFVPAVGPIAGAL
IMGIWGWRAIFLALGIPALIVLTLMLPAWHETRQAKGGQDSAGFRPVLRSPAFWTYTIGFGTAMGTFFVFFSTAPRVLID
GAGFSELDFSFAFASVAIAMIITTRFAKSFVARWGIPGTLMRGMVMLILGAFLLAIGQLFLAPSFWSLVVPMWLIAIGIV
FTTAVTANGALQEFGETAGMAVALHFCIQSLLVGIVGTGFVIMLDGSTAWPLITNAAGMAAVTLIALFWLGGRNLHPQTA

Specific function: Appears to provoke a reduction of the content of the major porins ompA and ompC [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI48994889, Length=383, Percent_Identity=26.8929503916449, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1788509, Length=372, Percent_Identity=27.6881720430108, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1790146, Length=365, Percent_Identity=28.7671232876712, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1790794, Length=326, Percent_Identity=26.6871165644172, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87082312, Length=337, Percent_Identity=26.7062314540059, Blast_Score=85, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6324264, Length=193, Percent_Identity=27.979274611399, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6322071, Length=182, Percent_Identity=29.6703296703297, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42528; Mature: 42396

Theoretical pI: Translated: 10.25; Mature: 10.25

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRNIRLWTYSLPVALMLLAPFNILASLGMDIYLPAIPAMPGILGTTPNVVQLTLTLYMI
CCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCEEECCHHCCCCCCCCCCHHHHHHHHHHHH
MLGVGQILFGPLSDRLGRRPVLIGGAILFATASFALAATSSAGLFLLLRLAQATGAAAVL
HHHHHHHHHCCHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHH
VALFATIRDVYAERPESAVIYSLMNAMLAFVPAVGPIAGALIMGIWGWRAIFLALGIPAL
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVLTLMLPAWHETRQAKGGQDSAGFRPVLRSPAFWTYTIGFGTAMGTFFVFFSTAPRVLI
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCEEEEEEHHHHHHHHHHHHHHCCCCEEE
DGAGFSELDFSFAFASVAIAMIITTRFAKSFVARWGIPGTLMRGMVMLILGAFLLAIGQL
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
FLAPSFWSLVVPMWLIAIGIVFTTAVTANGALQEFGETAGMAVALHFCIQSLLVGIVGTG
HHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
FVIMLDGSTAWPLITNAAGMAAVTLIALFWLGGRNLHPQTA
EEEEECCCCCCCHHHCCHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SRNIRLWTYSLPVALMLLAPFNILASLGMDIYLPAIPAMPGILGTTPNVVQLTLTLYMI
CCCEEEEEEHHHHHHHHHHHHHHHHHCCCCEEECCHHCCCCCCCCCCHHHHHHHHHHHH
MLGVGQILFGPLSDRLGRRPVLIGGAILFATASFALAATSSAGLFLLLRLAQATGAAAVL
HHHHHHHHHCCHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHH
VALFATIRDVYAERPESAVIYSLMNAMLAFVPAVGPIAGALIMGIWGWRAIFLALGIPAL
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVLTLMLPAWHETRQAKGGQDSAGFRPVLRSPAFWTYTIGFGTAMGTFFVFFSTAPRVLI
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCEEEEEEHHHHHHHHHHHHHHCCCCEEE
DGAGFSELDFSFAFASVAIAMIITTRFAKSFVARWGIPGTLMRGMVMLILGAFLLAIGQL
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
FLAPSFWSLVVPMWLIAIGIVFTTAVTANGALQEFGETAGMAVALHFCIQSLLVGIVGTG
HHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
FVIMLDGSTAWPLITNAAGMAAVTLIALFWLGGRNLHPQTA
EEEEECCCCCCCHHHCCHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1648560; 1658833 [H]