BacMap

Definition	Mesorhizobium sp. BNC1, complete genome.
Accession	NC_008254
Length	4,412,446

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The map label for this gene is yqhE [C]

Identifier: 110636224

GI number: 110636224

Start: 4202497

End: 4203423

Strand: Reverse

Name: yqhE [C]

Synonym: Meso_3900

Alternate gene names: 110636224

Gene position: 4203423-4202497 (Counterclockwise)

Preceding gene: 110636225

Following gene: 110636223

Centisome position: 95.26

GC content: 61.81

Gene sequence:

>927_bases
ATGAAGACATACAAACTTGTAAACCGCGACGTTTCAGCCCCGGGTCGCGACAGTGCGGCGGACGCCGACGGCTCCGGCGT
CGAGATGCCGATGATCGGGCTCGGTGTTTTTCAGACGCCGCCCGACGAAACGACGGCTGCGGTCGAAGAAGCCTTGCGGC
TTGGCTATCGTCATATCGACACGGCCGCCGCCTATGGCAACGAGCGTGAGGTGGGCGAAGGTATCCGTCGCGCCGGCATC
GCGCGTGACGAGGTTTTTATTGAGACCAAGATCTGGATCAGCGACTACGGCTACGACGAAACCCTGCACGCGTTCGACAA
GAGCGCGGGCAAGCTTGGCGTCGATCAAATCGATCTCTTGCTTCTGCATCAGCCGCTCCCCTCCGCATTCGATCGCACCC
TTGGCGCCTATCGGGCGCTCGAAAAGCTGCTGGCCGATGGCAAGGTGCGCGCGATCGGCGTCAGCAACTTCATGCCCGAG
CATCTGACGCGCCTGTTGACCGAAACATCCATCGTGCCTGCGGTGAACCAGATCGAAGTCCACCCCTATTTCCAGCAGAG
GGCATTGCAGCGCCTGCATGCCGAGCACGGCATCCTCACGCAAGCCTGGTCACCCATCGGCGGCATTACCTTCTATAGGG
GCGGCGAGAAAAGCACGCTCGAAGACCCGGTGCTGCTGGAAATCGCGCAGCAGCACGGCAAGTCGGCCGCCCAGGTGATG
CTGCGCTGGCATTTGCAGGAAGGCCGTTCCGTCATTCCCAAGTCGACAAAACCGGCGCGCATCGCGGAGAATTTCGATGT
CTTCGACTTCGAGCTTTCGGGAGAGCAGATCGCCGCCATCGATGCGCTCGATACCGGTGTCCGCAGCGGCCCGGAGCCCG
ACAGCATCACGCTCGAGAGCTATGGCAGGCCGATCCCCGAGGCGTAA

Upstream 100 bases:

>100_bases
TCCGAGCTCGATGCCCAGCGCACCGAACTCGAAGCATGGCGGGACGTCAGCACAAGCACCGACTTTCCGACCGGCACCAG
ATCATGAATCGAGAAGAACG

Downstream 100 bases:

>100_bases
TCCGGCGCGCGGGCCGGCCTGCGGCTCACCGCCGCGCAGGGCACGATGCCTGCCGGAAGCCGTGCTCGAAGATGGCAGGG
TTCTGATCAACAGACGGGAA

Product: aldo/keto reductase

Products: morphinone; NADH; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MKTYKLVNRDVSAPGRDSAADADGSGVEMPMIGLGVFQTPPDETTAAVEEALRLGYRHIDTAAAYGNEREVGEGIRRAGI
ARDEVFIETKIWISDYGYDETLHAFDKSAGKLGVDQIDLLLLHQPLPSAFDRTLGAYRALEKLLADGKVRAIGVSNFMPE
HLTRLLTETSIVPAVNQIEVHPYFQQRALQRLHAEHGILTQAWSPIGGITFYRGGEKSTLEDPVLLEIAQQHGKSAAQVM
LRWHLQEGRSVIPKSTKPARIAENFDVFDFELSGEQIAAIDALDTGVRSGPEPDSITLESYGRPIPEA

Sequences:

>Translated_308_residues
MKTYKLVNRDVSAPGRDSAADADGSGVEMPMIGLGVFQTPPDETTAAVEEALRLGYRHIDTAAAYGNEREVGEGIRRAGI
ARDEVFIETKIWISDYGYDETLHAFDKSAGKLGVDQIDLLLLHQPLPSAFDRTLGAYRALEKLLADGKVRAIGVSNFMPE
HLTRLLTETSIVPAVNQIEVHPYFQQRALQRLHAEHGILTQAWSPIGGITFYRGGEKSTLEDPVLLEIAQQHGKSAAQVM
LRWHLQEGRSVIPKSTKPARIAENFDVFDFELSGEQIAAIDALDTGVRSGPEPDSITLESYGRPIPEA
>Mature_308_residues
MKTYKLVNRDVSAPGRDSAADADGSGVEMPMIGLGVFQTPPDETTAAVEEALRLGYRHIDTAAAYGNEREVGEGIRRAGI
ARDEVFIETKIWISDYGYDETLHAFDKSAGKLGVDQIDLLLLHQPLPSAFDRTLGAYRALEKLLADGKVRAIGVSNFMPE
HLTRLLTETSIVPAVNQIEVHPYFQQRALQRLHAEHGILTQAWSPIGGITFYRGGEKSTLEDPVLLEIAQQHGKSAAQVM
LRWHLQEGRSVIPKSTKPARIAENFDVFDFELSGEQIAAIDALDTGVRSGPEPDSITLESYGRPIPEA

Specific function: Reduction [C]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI24497577, Length=288, Percent_Identity=36.4583333333333, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI5174391, Length=288, Percent_Identity=36.4583333333333, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI223468663, Length=303, Percent_Identity=36.6336633663366, Blast_Score=189, Evalue=3e-48,
Organism=Homo sapiens, GI4502049, Length=291, Percent_Identity=37.4570446735395, Blast_Score=178, Evalue=7e-45,
Organism=Homo sapiens, GI93277124, Length=317, Percent_Identity=33.4384858044164, Blast_Score=168, Evalue=7e-42,
Organism=Homo sapiens, GI291291012, Length=268, Percent_Identity=36.1940298507463, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI310109922, Length=277, Percent_Identity=33.2129963898917, Blast_Score=159, Evalue=2e-39,
Organism=Homo sapiens, GI5174695, Length=299, Percent_Identity=32.1070234113712, Blast_Score=157, Evalue=9e-39,
Organism=Homo sapiens, GI5453543, Length=289, Percent_Identity=32.1799307958478, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI310109920, Length=289, Percent_Identity=32.1799307958478, Blast_Score=155, Evalue=4e-38,
Organism=Homo sapiens, GI45446745, Length=289, Percent_Identity=32.1799307958478, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI4503285, Length=289, Percent_Identity=32.1799307958478, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI24497583, Length=293, Percent_Identity=32.0819112627986, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI24497585, Length=294, Percent_Identity=32.312925170068, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI300116273, Length=274, Percent_Identity=31.3868613138686, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI300116271, Length=278, Percent_Identity=32.0143884892086, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI310109924, Length=154, Percent_Identity=37.012987012987, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI310109926, Length=105, Percent_Identity=31.4285714285714, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI207028673, Length=105, Percent_Identity=31.4285714285714, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI87082198, Length=271, Percent_Identity=44.6494464944649, Blast_Score=241, Evalue=3e-65,
Organism=Escherichia coli, GI1786400, Length=266, Percent_Identity=37.218045112782, Blast_Score=155, Evalue=3e-39,
Organism=Escherichia coli, GI1788081, Length=280, Percent_Identity=31.4285714285714, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787674, Length=277, Percent_Identity=28.158844765343, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI48994888, Length=229, Percent_Identity=27.9475982532751, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71998625, Length=270, Percent_Identity=38.1481481481481, Blast_Score=192, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI17537077, Length=309, Percent_Identity=35.5987055016181, Blast_Score=187, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI17537075, Length=289, Percent_Identity=37.0242214532872, Blast_Score=187, Evalue=7e-48,
Organism=Caenorhabditis elegans, GI17561298, Length=289, Percent_Identity=35.9861591695502, Blast_Score=179, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17538386, Length=274, Percent_Identity=34.6715328467153, Blast_Score=166, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17537079, Length=294, Percent_Identity=31.6326530612245, Blast_Score=157, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI17552492, Length=277, Percent_Identity=32.129963898917, Blast_Score=157, Evalue=7e-39,
Organism=Caenorhabditis elegans, GI17564128, Length=296, Percent_Identity=32.7702702702703, Blast_Score=155, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17550248, Length=283, Percent_Identity=33.9222614840989, Blast_Score=154, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI17566692, Length=283, Percent_Identity=31.8021201413428, Blast_Score=151, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI17562292, Length=289, Percent_Identity=30.4498269896194, Blast_Score=136, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17561300, Length=276, Percent_Identity=29.3478260869565, Blast_Score=123, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6324694, Length=287, Percent_Identity=34.8432055749129, Blast_Score=179, Evalue=6e-46,
Organism=Saccharomyces cerevisiae, GI6320576, Length=280, Percent_Identity=33.5714285714286, Blast_Score=170, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6322556, Length=269, Percent_Identity=35.3159851301115, Blast_Score=166, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6321896, Length=304, Percent_Identity=33.2236842105263, Blast_Score=160, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6319625, Length=281, Percent_Identity=34.1637010676157, Blast_Score=151, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6320079, Length=277, Percent_Identity=32.4909747292419, Blast_Score=129, Evalue=6e-31,
Organism=Drosophila melanogaster, GI24644950, Length=297, Percent_Identity=38.7205387205387, Blast_Score=193, Evalue=1e-49,
Organism=Drosophila melanogaster, GI24662789, Length=291, Percent_Identity=37.4570446735395, Blast_Score=191, Evalue=7e-49,
Organism=Drosophila melanogaster, GI24663317, Length=297, Percent_Identity=36.026936026936, Blast_Score=186, Evalue=1e-47,
Organism=Drosophila melanogaster, GI221378297, Length=316, Percent_Identity=36.7088607594937, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24657054, Length=307, Percent_Identity=35.8306188925081, Blast_Score=182, Evalue=2e-46,
Organism=Drosophila melanogaster, GI20129731, Length=288, Percent_Identity=36.4583333333333, Blast_Score=170, Evalue=9e-43,
Organism=Drosophila melanogaster, GI21356425, Length=303, Percent_Identity=33.993399339934, Blast_Score=170, Evalue=9e-43,
Organism=Drosophila melanogaster, GI45553081, Length=291, Percent_Identity=35.7388316151203, Blast_Score=166, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24662781, Length=304, Percent_Identity=35.5263157894737, Blast_Score=152, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24662785, Length=289, Percent_Identity=36.3321799307958, Blast_Score=152, Evalue=2e-37,
Organism=Drosophila melanogaster, GI281366140, Length=261, Percent_Identity=35.2490421455939, Blast_Score=142, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: 1.1.1.218

Molecular weight: Translated: 33815; Mature: 33815

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2 ; PS00063 ALDOKETO_REDUCTASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTYKLVNRDVSAPGRDSAADADGSGVEMPMIGLGVFQTPPDETTAAVEEALRLGYRHID
CCCHHHHCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
TAAAYGNEREVGEGIRRAGIARDEVFIETKIWISDYGYDETLHAFDKSAGKLGVDQIDLL
HHHHCCCHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCHHHHHHHHCCCCCCHHHHHHH
LLHQPLPSAFDRTLGAYRALEKLLADGKVRAIGVSNFMPEHLTRLLTETSIVPAVNQIEV
EECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECHHCCHHHHHHHHHHHHHCCCCCEEEE
HPYFQQRALQRLHAEHGILTQAWSPIGGITFYRGGEKSTLEDPVLLEIAQQHGKSAAQVM
CHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCHHHHHH
LRWHLQEGRSVIPKSTKPARIAENFDVFDFELSGEQIAAIDALDTGVRSGPEPDSITLES
HHHHHHHCCCCCCCCCCCCHHHCCCCEEEEEECCCEEEEHHHHHHHHCCCCCCCEEEHHH
YGRPIPEA
CCCCCCCC
>Mature Secondary Structure
MKTYKLVNRDVSAPGRDSAADADGSGVEMPMIGLGVFQTPPDETTAAVEEALRLGYRHID
CCCHHHHCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
TAAAYGNEREVGEGIRRAGIARDEVFIETKIWISDYGYDETLHAFDKSAGKLGVDQIDLL
HHHHCCCHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCHHHHHHHHCCCCCCHHHHHHH
LLHQPLPSAFDRTLGAYRALEKLLADGKVRAIGVSNFMPEHLTRLLTETSIVPAVNQIEV
EECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECHHCCHHHHHHHHHHHHHCCCCCEEEE
HPYFQQRALQRLHAEHGILTQAWSPIGGITFYRGGEKSTLEDPVLLEIAQQHGKSAAQVM
CHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCHHHHHH
LRWHLQEGRSVIPKSTKPARIAENFDVFDFELSGEQIAAIDALDTGVRSGPEPDSITLES
HHHHHHHCCCCCCCCCCCCHHHCCCCEEEEEECCCEEEEHHHHHHHHCCCCCCCEEEHHH
YGRPIPEA
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: morphine; NAD+; NADP+

Specific reaction: morphine + NAD(P)+ = morphinone + NAD(P)H + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA