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The map label for this gene is dapE

Identifier: 110632750

GI number: 110632750

Start: 428977

End: 430164

Strand: Direct

Name: dapE

Synonym: Meso_0389

Alternate gene names: 110632750

Gene position: 428977-430164 (Clockwise)

Preceding gene: 110632749

Following gene: 110632751

Centisome position: 9.72

GC content: 63.38

Gene sequence:

>1188_bases
ATGACGCTCCCAACCGATCCCGCCCAGAATCTCGCCTCCCTCATTCGTTGCCGGTCGGTTACCCCGGTGGAGGGCGGAGC
GCTGACCGCGCTTGAAGCGATGCTTCGCCCGCTCGGGGCTCGCGTGGATCGGCCCGTCTTCTCGGAGGAGGGCGAAGCGG
ATGTGGAGAACCTCTATGCCCGGATCGGCAAAGACGGCCCGCATCTGATGTTTGCAGGGCACACGGATGTGGTGCCGCCC
GGTGACGAGGACGCCTGGACGCATCCGCCCTTTGCCGCCGAAATCGCCGGCGGCGAGATGTTCGGCCGCGGTGCCGTCGA
TATGAAGGGCGGGATCGCCTGCTTCGTCGCCGCACTCGCCCGTCATCTCGAAGTCAGTGGCACCCCGAAAGGCTCCGTCT
CATTCCTGATCACGGGGGACGAGGAGGGTCCGTCGGTCAACGGCTCGGTGAAGCTGCTCGAATGGGCGGCGGCGCGCGGA
GAGCGTTGGGATGCCGCATTGGTCGGTGAGCCCACGAATGTGGAAACGCTCGGCGACATGGTGAAGATCGGCCGGCGCGG
CTCCCTATCGGGCCGGATCACCCTTTTCGGCAGGCAGGGACACGTAGCCTACCCGCATCTGGCGGATAATCCGTCACGGG
GGCTGGTCGCTTTGTTGGAAGCTCTGATGGAGCCCGCGTTCGATCAGGGCACTGCCGATTTTCCGCCTACGAATCTGGAA
ATCACCACGATCGATGTGGGCAATCCTTCGGTGAATGTCATTCCCGCCCGCGCGACGGCCGCCTTCAACGTTCGCTTCAA
CGACACCTGGTCGGTGGAAACGCTGCAGGCGGAAATTCATAACCGGCTCGACCGTGCCGCGGCGGAAAATCGGTTGCGAC
CGGGCCGCACCGAGCCTGTCTCCTTCGAGCTGGAGTGGCGCGACCGGCCGAGCCCCGTATTCCTGACCCGTGACGACAAG
CTTGTTGCGACGCTCGCCGCCTCGATAGAAGCCGTGACGGGCCGCAAGCCGCAGCTCTCCACGACCGGAGGCACATCGGA
CGCGCGTTTTATCAAGGACTATTGTCCTGTAATTGAATTCGGCCTTGTCGGGCAAACCATGCACATGGTGGATGAGAAGG
TTCCGCTCTCCGATCTGGAGACGCTGACACGAATCTATCAGCGCTTTCTCGAGAACTGGTTCTCCTGA

Upstream 100 bases:

>100_bases
CGATGCCTCGGCCAATCAATTTGGAGAGCGGACCAATGCGCCGCAATGAAGTCCCTTACGGCGGTGACAGAACGGTCCTC
TTCAAGTATCCATCCCGGTC

Downstream 100 bases:

>100_bases
TGCCCTCCGGCGCGGAAATCCAACAATATTTCACCGGAGTCTGGCAGATGATGATGGGCCGAGCCGACGGCCTGAAGATG
CTCGATCTTTCGGCGGACGG

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MTLPTDPAQNLASLIRCRSVTPVEGGALTALEAMLRPLGARVDRPVFSEEGEADVENLYARIGKDGPHLMFAGHTDVVPP
GDEDAWTHPPFAAEIAGGEMFGRGAVDMKGGIACFVAALARHLEVSGTPKGSVSFLITGDEEGPSVNGSVKLLEWAAARG
ERWDAALVGEPTNVETLGDMVKIGRRGSLSGRITLFGRQGHVAYPHLADNPSRGLVALLEALMEPAFDQGTADFPPTNLE
ITTIDVGNPSVNVIPARATAAFNVRFNDTWSVETLQAEIHNRLDRAAAENRLRPGRTEPVSFELEWRDRPSPVFLTRDDK
LVATLAASIEAVTGRKPQLSTTGGTSDARFIKDYCPVIEFGLVGQTMHMVDEKVPLSDLETLTRIYQRFLENWFS

Sequences:

>Translated_395_residues
MTLPTDPAQNLASLIRCRSVTPVEGGALTALEAMLRPLGARVDRPVFSEEGEADVENLYARIGKDGPHLMFAGHTDVVPP
GDEDAWTHPPFAAEIAGGEMFGRGAVDMKGGIACFVAALARHLEVSGTPKGSVSFLITGDEEGPSVNGSVKLLEWAAARG
ERWDAALVGEPTNVETLGDMVKIGRRGSLSGRITLFGRQGHVAYPHLADNPSRGLVALLEALMEPAFDQGTADFPPTNLE
ITTIDVGNPSVNVIPARATAAFNVRFNDTWSVETLQAEIHNRLDRAAAENRLRPGRTEPVSFELEWRDRPSPVFLTRDDK
LVATLAASIEAVTGRKPQLSTTGGTSDARFIKDYCPVIEFGLVGQTMHMVDEKVPLSDLETLTRIYQRFLENWFS
>Mature_394_residues
TLPTDPAQNLASLIRCRSVTPVEGGALTALEAMLRPLGARVDRPVFSEEGEADVENLYARIGKDGPHLMFAGHTDVVPPG
DEDAWTHPPFAAEIAGGEMFGRGAVDMKGGIACFVAALARHLEVSGTPKGSVSFLITGDEEGPSVNGSVKLLEWAAARGE
RWDAALVGEPTNVETLGDMVKIGRRGSLSGRITLFGRQGHVAYPHLADNPSRGLVALLEALMEPAFDQGTADFPPTNLEI
TTIDVGNPSVNVIPARATAAFNVRFNDTWSVETLQAEIHNRLDRAAAENRLRPGRTEPVSFELEWRDRPSPVFLTRDDKL
VATLAASIEAVTGRKPQLSTTGGTSDARFIKDYCPVIEFGLVGQTMHMVDEKVPLSDLETLTRIYQRFLENWFS

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily

Homologues:

Organism=Escherichia coli, GI1788816, Length=381, Percent_Identity=43.0446194225722, Blast_Score=280, Evalue=2e-76,
Organism=Escherichia coli, GI1790395, Length=192, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPE_MESSB (Q11LD2)

Other databases:

- EMBL:   CP000390
- RefSeq:   YP_672958.1
- ProteinModelPortal:   Q11LD2
- SMR:   Q11LD2
- STRING:   Q11LD2
- MEROPS:   M20.010
- GeneID:   4182067
- GenomeReviews:   CP000390_GR
- KEGG:   mes:Meso_0389
- NMPDR:   fig|266779.1.peg.4187
- eggNOG:   COG0624
- HOGENOM:   HBG728841
- OMA:   ARNPVHQ
- PhylomeDB:   Q11LD2
- ProtClustDB:   PRK13009
- BioCyc:   MSP266779:MESO_0389-MONOMER
- GO:   GO:0006508
- HAMAP:   MF_01690
- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650
- TIGRFAMs:   TIGR01246

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.18

Molecular weight: Translated: 42768; Mature: 42637

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 76-76 ACT_SITE 141-141

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLPTDPAQNLASLIRCRSVTPVEGGALTALEAMLRPLGARVDRPVFSEEGEADVENLYA
CCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
RIGKDGPHLMFAGHTDVVPPGDEDAWTHPPFAAEIAGGEMFGRGAVDMKGGIACFVAALA
HHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEECCCCEECCCCCCCCCCHHHHHHHHH
RHLEVSGTPKGSVSFLITGDEEGPSVNGSVKLLEWAAARGERWDAALVGEPTNVETLGDM
HHHCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEEHHCCCCCCCEEEECCCCCHHHHHHH
VKIGRRGSLSGRITLFGRQGHVAYPHLADNPSRGLVALLEALMEPAFDQGTADFPPTNLE
HHHCCCCCCCCEEEEEECCCCEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEE
ITTIDVGNPSVNVIPARATAAFNVRFNDTWSVETLQAEIHNRLDRAAAENRLRPGRTEPV
EEEEECCCCCEEEEECCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
SFELEWRDRPSPVFLTRDDKLVATLAASIEAVTGRKPQLSTTGGTSDARFIKDYCPVIEF
EEEEEECCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
GLVGQTMHMVDEKVPLSDLETLTRIYQRFLENWFS
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TLPTDPAQNLASLIRCRSVTPVEGGALTALEAMLRPLGARVDRPVFSEEGEADVENLYA
CCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
RIGKDGPHLMFAGHTDVVPPGDEDAWTHPPFAAEIAGGEMFGRGAVDMKGGIACFVAALA
HHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEECCCCEECCCCCCCCCCHHHHHHHHH
RHLEVSGTPKGSVSFLITGDEEGPSVNGSVKLLEWAAARGERWDAALVGEPTNVETLGDM
HHHCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEEHHCCCCCCCEEEECCCCCHHHHHHH
VKIGRRGSLSGRITLFGRQGHVAYPHLADNPSRGLVALLEALMEPAFDQGTADFPPTNLE
HHHCCCCCCCCEEEEEECCCCEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEE
ITTIDVGNPSVNVIPARATAAFNVRFNDTWSVETLQAEIHNRLDRAAAENRLRPGRTEPV
EEEEECCCCCEEEEECCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
SFELEWRDRPSPVFLTRDDKLVATLAASIEAVTGRKPQLSTTGGTSDARFIKDYCPVIEF
EEEEEECCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
GLVGQTMHMVDEKVPLSDLETLTRIYQRFLENWFS
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA