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The map label for this gene is ycdT [H]

Identifier: 110632742

GI number: 110632742

Start: 423249

End: 424004

Strand: Reverse

Name: ycdT [H]

Synonym: Meso_0381

Alternate gene names: 110632742

Gene position: 424004-423249 (Counterclockwise)

Preceding gene: 110632744

Following gene: 110632740

Centisome position: 9.61

GC content: 62.83

Gene sequence:

>756_bases
ATGAAGAGGGTCTTCTACCACGCTTCGGTGGCCTCGTTCATTTCCATCGCCGTCTCCCTGGCCATCTGCTGGGCGGCGCT
AAAGGCCGTGGGCAGCGACCTGGATTTTTTCGCGGCCGCGATGTGCGTGGTTTGCCCGCTCGTCATCGCCTTCCCCGCAA
GCGCCTTTACATACAGCCAGAAGCTCAAGATCGCAGAAGCGCACAAGGCGCTGGAAGCTGCTCATAACGAGCTTGCGGCC
ATGCATCAAAAACTGGCGGAACGCGCGCGGCTAGACGATATGACGGGACTATTGAACCGCAGCGCCTTCATAGCCTCGGT
CGAAGAGACCCGCGTGAGCGAGGGCCGCGGCTGCCTCATCCTGATCGATGCCGATAGATTCAAGCAGATCAACGATACGT
TCGGGCATCTGGCGGGAGATCTCGCGCTCATCGAAATCGCCGGAGCCATTCGCGGCGCCAGCGGGCAGATGCATGCGATC
GGACGCCTCGGCGGAGAAGAGTTCGGCGTCTTCGCCTTCTCTTGCAGCCTCATCGAGGCGCGAAAGCTTGCCGAAGAAAT
CCGCGCGGCGGTCGAGAAAACGCACTTCGCGCCGGATGGAACAGAACCCATTCCACTCACCGTCAGCATCGGCGTGGTGG
CGGCGCGCACCGGGGCGGGCTTTTCCGAGATCATGGGCGAGGCCGACCAGCGGCTCTATCAGGCCAAGCGGCGCGGCCGT
AACACAGTGGTGGCCGGCTCACGCGCCGCGGCGTAA

Upstream 100 bases:

>100_bases
TTGTCATGGCCTCTTTATGGCAGGTTTCGCGGCGGGGTTCCAAACCCTCAATAAGCGAACAACACGAAATTCGGTTGCCG
AAGGTCATGCGGATTCCGTG

Downstream 100 bases:

>100_bases
CGCCGGTTCACCTTCATGGGACGATCAGCGTTCCCGCCCCGTGCTCCGTGAACAGTTCCAAAAGCACCGAATGGGCCGTC
TTGCCGTTGAGGATGACCAC

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MKRVFYHASVASFISIAVSLAICWAALKAVGSDLDFFAAAMCVVCPLVIAFPASAFTYSQKLKIAEAHKALEAAHNELAA
MHQKLAERARLDDMTGLLNRSAFIASVEETRVSEGRGCLILIDADRFKQINDTFGHLAGDLALIEIAGAIRGASGQMHAI
GRLGGEEFGVFAFSCSLIEARKLAEEIRAAVEKTHFAPDGTEPIPLTVSIGVVAARTGAGFSEIMGEADQRLYQAKRRGR
NTVVAGSRAAA

Sequences:

>Translated_251_residues
MKRVFYHASVASFISIAVSLAICWAALKAVGSDLDFFAAAMCVVCPLVIAFPASAFTYSQKLKIAEAHKALEAAHNELAA
MHQKLAERARLDDMTGLLNRSAFIASVEETRVSEGRGCLILIDADRFKQINDTFGHLAGDLALIEIAGAIRGASGQMHAI
GRLGGEEFGVFAFSCSLIEARKLAEEIRAAVEKTHFAPDGTEPIPLTVSIGVVAARTGAGFSEIMGEADQRLYQAKRRGR
NTVVAGSRAAA
>Mature_251_residues
MKRVFYHASVASFISIAVSLAICWAALKAVGSDLDFFAAAMCVVCPLVIAFPASAFTYSQKLKIAEAHKALEAAHNELAA
MHQKLAERARLDDMTGLLNRSAFIASVEETRVSEGRGCLILIDADRFKQINDTFGHLAGDLALIEIAGAIRGASGQMHAI
GRLGGEEFGVFAFSCSLIEARKLAEEIRAAVEKTHFAPDGTEPIPLTVSIGVVAARTGAGFSEIMGEADQRLYQAKRRGR
NTVVAGSRAAA

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=158, Percent_Identity=36.0759493670886, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI87081881, Length=193, Percent_Identity=35.2331606217617, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787802, Length=204, Percent_Identity=29.9019607843137, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI145693134, Length=158, Percent_Identity=34.8101265822785, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI87082007, Length=179, Percent_Identity=34.6368715083799, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI1788381, Length=182, Percent_Identity=32.967032967033, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788085, Length=172, Percent_Identity=34.3023255813954, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1788956, Length=192, Percent_Identity=30.2083333333333, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1787056, Length=160, Percent_Identity=33.125, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1786584, Length=246, Percent_Identity=27.6422764227642, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787816, Length=159, Percent_Identity=32.7044025157233, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI87081974, Length=166, Percent_Identity=30.7228915662651, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87081977, Length=176, Percent_Identity=28.9772727272727, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 26724; Mature: 26724

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRVFYHASVASFISIAVSLAICWAALKAVGSDLDFFAAAMCVVCPLVIAFPASAFTYSQ
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHH
KLKIAEAHKALEAAHNELAAMHQKLAERARLDDMTGLLNRSAFIASVEETRVSEGRGCLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
LIDADRFKQINDTFGHLAGDLALIEIAGAIRGASGQMHAIGRLGGEEFGVFAFSCSLIEA
EEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHHHHHHH
RKLAEEIRAAVEKTHFAPDGTEPIPLTVSIGVVAARTGAGFSEIMGEADQRLYQAKRRGR
HHHHHHHHHHHHHHCCCCCCCCCCEEEEEECEEEECCCCCHHHHHHHHHHHHHHHHHCCC
NTVVAGSRAAA
CEEECCCCCCC
>Mature Secondary Structure
MKRVFYHASVASFISIAVSLAICWAALKAVGSDLDFFAAAMCVVCPLVIAFPASAFTYSQ
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHH
KLKIAEAHKALEAAHNELAAMHQKLAERARLDDMTGLLNRSAFIASVEETRVSEGRGCLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
LIDADRFKQINDTFGHLAGDLALIEIAGAIRGASGQMHAIGRLGGEEFGVFAFSCSLIEA
EEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHHHHHHH
RKLAEEIRAAVEKTHFAPDGTEPIPLTVSIGVVAARTGAGFSEIMGEADQRLYQAKRRGR
HHHHHHHHHHHHHHCCCCCCCCCCEEEEEECEEEECCCCCHHHHHHHHHHHHHHHHHCCC
NTVVAGSRAAA
CEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]