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The map label for this gene is ntrB [H]

Identifier: 110632712

GI number: 110632712

Start: 392664

End: 394190

Strand: Reverse

Name: ntrB [H]

Synonym: Meso_0351

Alternate gene names: 110632712

Gene position: 394190-392664 (Counterclockwise)

Preceding gene: 110632713

Following gene: 110632709

Centisome position: 8.93

GC content: 61.03

Gene sequence:

>1527_bases
ATGGCCGAGGAGAAGCGTCAGGAAGGAACCAAGACCGAATTAGACGCGAGTCGGGCCGTGCCACTGACCCGCGGTCTCTC
GACGAAATTGCTTTTCCTGACCATCCTCTTTGTCATGGCGGCGGAGGTGCTGATCTTTATCCCGTCGGTCGCCAATTTCG
GCCGGCAGTGGATGGAGCAGCGGCTGCGCGAGGCAGCGATCGTCGGCCGTGTCCTCCTCGATGGAGAGGCGGGCAATCTC
AGCCGGGAGGCTAGCAACGACGTTCTCATGACGGCGGGCGTGAGCGCCATCGCGGTGCGCGACGGGAACACGTCCCGCCT
CCTGGTCGTGTCGCATATGCCGCCGGCCGTCGACGCGCATATCGATCTTGACCGGTTCGATGCTTTAAGCGCCGTTCGGA
AGGCTTTCGGTACCCTGTTCTTTGGTGGCGGCCGCATCTTGCGCGTCTACGGCCATGTAGGACAGAGCACAGCGCAATAC
GAAGTCATCTTACCGGATCGTCAGCTGCGGGCGGCAATGCTGATTTACGCGCGCAATGTGGCGGCCATTTCATTTCTCAT
TTCGCTTTTCACCGCCACACTCGTTTTTTACGCAATCGACCGCATCATGATCGGACCAGTCCGCGCCATGACCCGCTCGG
TGCTCGCCTTCGGTGCGGCGCCGGATAATGCCGAAAACATCATCGAGCCGGAAAGCCGCAGCGACGAAATCGGCATCGCG
GAGCGGGAATTGGCAGAGATGCAGAAGGCACTGCACCGGACGCTTGGTGAGCGCAAACGGCTTGCGGAATTGGGCATGGC
GGTCTCCAAAATCAATCACGACATGCGCAACATGCTGGCGTCTGCGCAGCTCATTTCGGATCGCTTGGCCCTCAGCGCCG
ATCCAACGGTGCAATCTCTGGCTCCAAAACTCGTGCGCATGCTTGACCGGGCAGTGGCGTATTCCCAGGGAGTGATCGCC
TATGGCCGGGCGCAGGAGGCGCCACCCTCGCGCCGCCGGCTGCGTCTTGCTCCACTTGTTGAGGATGTGCACGCAAGCCT
TGCGATCGATCCGGGGAGCGGAATCGAATTCGTCAATGAAATCGATCCGCTATTCGAGATCGAAGCGGATGCCGACCAGC
TTTTCAGGGTGCTTTCCAACCTCTGCCGAAACGCCGTTCAGGCCATGACGGGCGAAGCGGATTCGGCGCTCGTACGGCGC
CTCACAATCTCGGGGCGCCAAGGGGAAGGACTGGCGCGAATTTTTGTCGCTGATACCGGGCCGGGGCTTCCGCCAAAAGC
GCGTGAAAACCTGTTCACAGCCTTCCGCGGCTCGGCTCGGACCGGCGGCACAGGCCTCGGGCTTGCCATCGCGCAGGAGC
TCATACGCGCGCATGGCGGAGAGATCGAGCTTATGGAAAGCGGAAACGGGCGTACGATCTTCTGTATTACCATTCCTGGT
CCGACCCCTGAGGACTTCCATCGGGAGAGCACGGAAAGCAAAAATGCCGCCACAGAGAATGGCCCAACGGCACCGCGGCA
GCAGTAA

Upstream 100 bases:

>100_bases
CGCAGCTCATCCGTGCGTGGAATATTAGGCTTTTTGCGGCTATTTGGCTAAGTAGGGTGATCATTGTGGGAACCACCACC
TGTGCAGCAGCGGAACAGGA

Downstream 100 bases:

>100_bases
TCCGCGGCAACCCGCATCGGCCTTCCGCCTGCGGATACGGCTCCGCCATCTTGGCTGGAGAATGTTTCGCTGGCAGCCGT
CCGTTCTGGGTAGGTGTGCT

Product: periplasmic sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 508; Mature: 507

Protein sequence:

>508_residues
MAEEKRQEGTKTELDASRAVPLTRGLSTKLLFLTILFVMAAEVLIFIPSVANFGRQWMEQRLREAAIVGRVLLDGEAGNL
SREASNDVLMTAGVSAIAVRDGNTSRLLVVSHMPPAVDAHIDLDRFDALSAVRKAFGTLFFGGGRILRVYGHVGQSTAQY
EVILPDRQLRAAMLIYARNVAAISFLISLFTATLVFYAIDRIMIGPVRAMTRSVLAFGAAPDNAENIIEPESRSDEIGIA
ERELAEMQKALHRTLGERKRLAELGMAVSKINHDMRNMLASAQLISDRLALSADPTVQSLAPKLVRMLDRAVAYSQGVIA
YGRAQEAPPSRRRLRLAPLVEDVHASLAIDPGSGIEFVNEIDPLFEIEADADQLFRVLSNLCRNAVQAMTGEADSALVRR
LTISGRQGEGLARIFVADTGPGLPPKARENLFTAFRGSARTGGTGLGLAIAQELIRAHGGEIELMESGNGRTIFCITIPG
PTPEDFHRESTESKNAATENGPTAPRQQ

Sequences:

>Translated_508_residues
MAEEKRQEGTKTELDASRAVPLTRGLSTKLLFLTILFVMAAEVLIFIPSVANFGRQWMEQRLREAAIVGRVLLDGEAGNL
SREASNDVLMTAGVSAIAVRDGNTSRLLVVSHMPPAVDAHIDLDRFDALSAVRKAFGTLFFGGGRILRVYGHVGQSTAQY
EVILPDRQLRAAMLIYARNVAAISFLISLFTATLVFYAIDRIMIGPVRAMTRSVLAFGAAPDNAENIIEPESRSDEIGIA
ERELAEMQKALHRTLGERKRLAELGMAVSKINHDMRNMLASAQLISDRLALSADPTVQSLAPKLVRMLDRAVAYSQGVIA
YGRAQEAPPSRRRLRLAPLVEDVHASLAIDPGSGIEFVNEIDPLFEIEADADQLFRVLSNLCRNAVQAMTGEADSALVRR
LTISGRQGEGLARIFVADTGPGLPPKARENLFTAFRGSARTGGTGLGLAIAQELIRAHGGEIELMESGNGRTIFCITIPG
PTPEDFHRESTESKNAATENGPTAPRQQ
>Mature_507_residues
AEEKRQEGTKTELDASRAVPLTRGLSTKLLFLTILFVMAAEVLIFIPSVANFGRQWMEQRLREAAIVGRVLLDGEAGNLS
REASNDVLMTAGVSAIAVRDGNTSRLLVVSHMPPAVDAHIDLDRFDALSAVRKAFGTLFFGGGRILRVYGHVGQSTAQYE
VILPDRQLRAAMLIYARNVAAISFLISLFTATLVFYAIDRIMIGPVRAMTRSVLAFGAAPDNAENIIEPESRSDEIGIAE
RELAEMQKALHRTLGERKRLAELGMAVSKINHDMRNMLASAQLISDRLALSADPTVQSLAPKLVRMLDRAVAYSQGVIAY
GRAQEAPPSRRRLRLAPLVEDVHASLAIDPGSGIEFVNEIDPLFEIEADADQLFRVLSNLCRNAVQAMTGEADSALVRRL
TISGRQGEGLARIFVADTGPGLPPKARENLFTAFRGSARTGGTGLGLAIAQELIRAHGGEIELMESGNGRTIFCITIPGP
TPEDFHRESTESKNAATENGPTAPRQQ

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=222, Percent_Identity=31.981981981982, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1788549, Length=243, Percent_Identity=27.1604938271605, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1786912, Length=134, Percent_Identity=35.0746268656716, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI87082128, Length=318, Percent_Identity=27.6729559748428, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI1790436, Length=119, Percent_Identity=32.7731092436975, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 55101; Mature: 54970

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEEKRQEGTKTELDASRAVPLTRGLSTKLLFLTILFVMAAEVLIFIPSVANFGRQWMEQ
CCCHHHHCCCCHHCCHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLREAAIVGRVLLDGEAGNLSREASNDVLMTAGVSAIAVRDGNTSRLLVVSHMPPAVDAH
HHHHHHHHHHHEECCCCCCCCCCCCCCEEEECCCEEEEEECCCCCEEEEEECCCCCCCCC
IDLDRFDALSAVRKAFGTLFFGGGRILRVYGHVGQSTAQYEVILPDRQLRAAMLIYARNV
CCHHHHHHHHHHHHHHHHEEECCCEEEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHH
AAISFLISLFTATLVFYAIDRIMIGPVRAMTRSVLAFGAAPDNAENIIEPESRSDEIGIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCH
ERELAEMQKALHRTLGERKRLAELGMAVSKINHDMRNMLASAQLISDRLALSADPTVQSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
APKLVRMLDRAVAYSQGVIAYGRAQEAPPSRRRLRLAPLVEDVHASLAIDPGSGIEFVNE
HHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHEEHHHHHHHHHHHEEECCCCCCHHHHH
IDPLFEIEADADQLFRVLSNLCRNAVQAMTGEADSALVRRLTISGRQGEGLARIFVADTG
CCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEEECCC
PGLPPKARENLFTAFRGSARTGGTGLGLAIAQELIRAHGGEIELMESGNGRTIFCITIPG
CCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEECCC
PTPEDFHRESTESKNAATENGPTAPRQQ
CCCHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AEEKRQEGTKTELDASRAVPLTRGLSTKLLFLTILFVMAAEVLIFIPSVANFGRQWMEQ
CCHHHHCCCCHHCCHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLREAAIVGRVLLDGEAGNLSREASNDVLMTAGVSAIAVRDGNTSRLLVVSHMPPAVDAH
HHHHHHHHHHHEECCCCCCCCCCCCCCEEEECCCEEEEEECCCCCEEEEEECCCCCCCCC
IDLDRFDALSAVRKAFGTLFFGGGRILRVYGHVGQSTAQYEVILPDRQLRAAMLIYARNV
CCHHHHHHHHHHHHHHHHEEECCCEEEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHH
AAISFLISLFTATLVFYAIDRIMIGPVRAMTRSVLAFGAAPDNAENIIEPESRSDEIGIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCH
ERELAEMQKALHRTLGERKRLAELGMAVSKINHDMRNMLASAQLISDRLALSADPTVQSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
APKLVRMLDRAVAYSQGVIAYGRAQEAPPSRRRLRLAPLVEDVHASLAIDPGSGIEFVNE
HHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHEEHHHHHHHHHHHEEECCCCCCHHHHH
IDPLFEIEADADQLFRVLSNLCRNAVQAMTGEADSALVRRLTISGRQGEGLARIFVADTG
CCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEEECCC
PGLPPKARENLFTAFRGSARTGGTGLGLAIAQELIRAHGGEIELMESGNGRTIFCITIPG
CCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEECCC
PTPEDFHRESTESKNAATENGPTAPRQQ
CCCHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2574025 [H]