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The map label for this gene is folD

Identifier: 110632514

GI number: 110632514

Start: 185429

End: 186325

Strand: Direct

Name: folD

Synonym: Meso_0152

Alternate gene names: 110632514

Gene position: 185429-186325 (Clockwise)

Preceding gene: 110632511

Following gene: 110632517

Centisome position: 4.2

GC content: 61.76

Gene sequence:

>897_bases
ATGGTGCAGATTATTGATGGAAAGGCCGTCGCTGCAACGATCCTGGAAAAGGTGACACGCGAAACTCAGGACCTCAAGGC
AAATGGCGTTACGCCCGGCCTGGCGGTCGTGATCGTGGGCGAGGATCCTGCAAGCCAGGTCTATGTGGCCTCCAAGGGGC
GGAAGGCAACCGAGTGCGGTTTTCACTCGGTACAGCATTCGCTGCCGGAGAACACCTCCGAAGAGGAGCTTGTTTCACTG
GTGGAAGCCCTCAACCGCGATCCGGCAATTCACGGCATCCTCGTCCAGCTTCCGCTTCCCAAGCAGATCGATTCTGAACG
CATTATCCACACGATCGCGCCGGAAAAGGATGTCGACGGCTTCCATATTCTGAATGTCGGCATGCTTGGCACCGGCGCGG
TCACCAAGGCCCTGGTGCCCTGCACGCCAGCCGGTGCGATGCTTCTTATCGAGGGCGTGCGCGGCAAGGATCTTTCGGGA
CTGTCCGCCGTCGTCATCGGCCGCTCGAATATCGTGGGCAAGCCAATGGCCAATCTGCTGCTCGCCGCCAACGCCACGGT
TACGATCGCGCACAGCCGAACCGCCGACCTGCCGGCGGCGTGCCGCGGCGCGGACATTCTGGTTGCGGCGGTTGGGCGGC
CGGAAATGGTGAAGGGCGATTGGATCAAGCCCGGCGCTACCGTTATCGACGTCGGCGTAAGCCGGATTCCCGCGCCCGAA
AAAGGCGAGGGCAAGATGAAGCTTGTTGGCGACGTGGCCTATGCCGAGGCGGAGAAGGTGGCCGGAGCTATCACGCCGGT
GCCCGGAGGTGTAGGCCCGATGACCATCGCCATGCTGATGGCCAATACGTTGGCGGCAGCTTCGAAAGCTTCCGGTCGCG
ATTTGCCTGCATTCTGA

Upstream 100 bases:

>100_bases
ACGCTAACGGCTGCGGCAATAAGCGGGGCGACCATGCCGTTGCACGCGCGGGCTTTTGCCGCTTATGTGAGTAATGGGCA
GCCGGAGGAGCGAGTTATCC

Downstream 100 bases:

>100_bases
AGCGAGCTTAAAAGAGCAGCATCCGCCACGCCAGTCCAGCAAGCGCGGCAGAGAAAAGGACCGCGGCAAGCGGCGCTCTG
AAAAGGAAAATCGCAAGAAG

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MVQIIDGKAVAATILEKVTRETQDLKANGVTPGLAVVIVGEDPASQVYVASKGRKATECGFHSVQHSLPENTSEEELVSL
VEALNRDPAIHGILVQLPLPKQIDSERIIHTIAPEKDVDGFHILNVGMLGTGAVTKALVPCTPAGAMLLIEGVRGKDLSG
LSAVVIGRSNIVGKPMANLLLAANATVTIAHSRTADLPAACRGADILVAAVGRPEMVKGDWIKPGATVIDVGVSRIPAPE
KGEGKMKLVGDVAYAEAEKVAGAITPVPGGVGPMTIAMLMANTLAAASKASGRDLPAF

Sequences:

>Translated_298_residues
MVQIIDGKAVAATILEKVTRETQDLKANGVTPGLAVVIVGEDPASQVYVASKGRKATECGFHSVQHSLPENTSEEELVSL
VEALNRDPAIHGILVQLPLPKQIDSERIIHTIAPEKDVDGFHILNVGMLGTGAVTKALVPCTPAGAMLLIEGVRGKDLSG
LSAVVIGRSNIVGKPMANLLLAANATVTIAHSRTADLPAACRGADILVAAVGRPEMVKGDWIKPGATVIDVGVSRIPAPE
KGEGKMKLVGDVAYAEAEKVAGAITPVPGGVGPMTIAMLMANTLAAASKASGRDLPAF
>Mature_298_residues
MVQIIDGKAVAATILEKVTRETQDLKANGVTPGLAVVIVGEDPASQVYVASKGRKATECGFHSVQHSLPENTSEEELVSL
VEALNRDPAIHGILVQLPLPKQIDSERIIHTIAPEKDVDGFHILNVGMLGTGAVTKALVPCTPAGAMLLIEGVRGKDLSG
LSAVVIGRSNIVGKPMANLLLAANATVTIAHSRTADLPAACRGADILVAAVGRPEMVKGDWIKPGATVIDVGVSRIPAPE
KGEGKMKLVGDVAYAEAEKVAGAITPVPGGVGPMTIAMLMANTLAAASKASGRDLPAF

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=291, Percent_Identity=47.4226804123711, Blast_Score=260, Evalue=1e-69,
Organism=Homo sapiens, GI222418558, Length=297, Percent_Identity=43.7710437710438, Blast_Score=208, Evalue=4e-54,
Organism=Homo sapiens, GI94721354, Length=297, Percent_Identity=44.1077441077441, Blast_Score=202, Evalue=2e-52,
Organism=Homo sapiens, GI36796743, Length=205, Percent_Identity=26.8292682926829, Blast_Score=78, Evalue=8e-15,
Organism=Escherichia coli, GI1786741, Length=286, Percent_Identity=51.7482517482517, Blast_Score=261, Evalue=4e-71,
Organism=Caenorhabditis elegans, GI17568735, Length=260, Percent_Identity=45.3846153846154, Blast_Score=212, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6321643, Length=297, Percent_Identity=44.4444444444444, Blast_Score=238, Evalue=1e-63,
Organism=Saccharomyces cerevisiae, GI6319558, Length=289, Percent_Identity=44.2906574394464, Blast_Score=238, Evalue=1e-63,
Organism=Saccharomyces cerevisiae, GI6322933, Length=305, Percent_Identity=24.5901639344262, Blast_Score=76, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24645718, Length=295, Percent_Identity=49.1525423728814, Blast_Score=253, Evalue=1e-67,
Organism=Drosophila melanogaster, GI17137370, Length=295, Percent_Identity=49.1525423728814, Blast_Score=253, Evalue=1e-67,
Organism=Drosophila melanogaster, GI62472483, Length=295, Percent_Identity=49.1525423728814, Blast_Score=252, Evalue=2e-67,
Organism=Drosophila melanogaster, GI45551871, Length=295, Percent_Identity=49.1525423728814, Blast_Score=252, Evalue=2e-67,
Organism=Drosophila melanogaster, GI17136816, Length=300, Percent_Identity=48.3333333333333, Blast_Score=248, Evalue=4e-66,
Organism=Drosophila melanogaster, GI17136818, Length=300, Percent_Identity=48.3333333333333, Blast_Score=247, Evalue=6e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_MESSB (Q11M18)

Other databases:

- EMBL:   CP000390
- RefSeq:   YP_672722.1
- ProteinModelPortal:   Q11M18
- SMR:   Q11M18
- STRING:   Q11M18
- GeneID:   4179615
- GenomeReviews:   CP000390_GR
- KEGG:   mes:Meso_0152
- NMPDR:   fig|266779.1.peg.3311
- eggNOG:   COG0190
- HOGENOM:   HBG328751
- OMA:   MIMIERI
- PhylomeDB:   Q11M18
- ProtClustDB:   PRK14188
- BioCyc:   MSP266779:MESO_0152-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 30669; Mature: 30669

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQIIDGKAVAATILEKVTRETQDLKANGVTPGLAVVIVGEDPASQVYVASKGRKATECG
CEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEECCCCCHHHHH
FHSVQHSLPENTSEEELVSLVEALNRDPAIHGILVQLPLPKQIDSERIIHTIAPEKDVDG
HHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCEEEEECCCCCCCC
FHILNVGMLGTGAVTKALVPCTPAGAMLLIEGVRGKDLSGLSAVVIGRSNIVGKPMANLL
EEEEEECCCCCCHHHHHHCCCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCCCCHHHHE
LAANATVTIAHSRTADLPAACRGADILVAAVGRPEMVKGDWIKPGATVIDVGVSRIPAPE
EECCCEEEEEECCCCCCCCCCCCCEEEEEECCCCCEECCCCCCCCCEEEECCHHHCCCCC
KGEGKMKLVGDVAYAEAEKVAGAITPVPGGVGPMTIAMLMANTLAAASKASGRDLPAF
CCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MVQIIDGKAVAATILEKVTRETQDLKANGVTPGLAVVIVGEDPASQVYVASKGRKATECG
CEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEECCCCCHHHHH
FHSVQHSLPENTSEEELVSLVEALNRDPAIHGILVQLPLPKQIDSERIIHTIAPEKDVDG
HHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCEEEEECCCCCCCC
FHILNVGMLGTGAVTKALVPCTPAGAMLLIEGVRGKDLSGLSAVVIGRSNIVGKPMANLL
EEEEEECCCCCCHHHHHHCCCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCCCCHHHHE
LAANATVTIAHSRTADLPAACRGADILVAAVGRPEMVKGDWIKPGATVIDVGVSRIPAPE
EECCCEEEEEECCCCCCCCCCCCCEEEEEECCCCCEECCCCCCCCCEEEECCHHHCCCCC
KGEGKMKLVGDVAYAEAEKVAGAITPVPGGVGPMTIAMLMANTLAAASKASGRDLPAF
CCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA