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The map label for this gene is fliA [H]

Identifier: 109947668

GI number: 109947668

Start: 986428

End: 987204

Strand: Reverse

Name: fliA [H]

Synonym: Hac_1136

Alternate gene names: 109947668

Gene position: 987204-986428 (Counterclockwise)

Preceding gene: 109947669

Following gene: 109947667

Centisome position: 63.53

GC content: 39.9

Gene sequence:

>777_bases
ATGATTTTGACGATGGAAAATAGAATGCCCCAAGAAATCCAACAAACTGAACCAAGTGAAAAAAGCGAAAAAAACAAAGA
AAAGGTTTTGAACGCTTATGACAAGCAACAACACCACCATCAAGACGCACTCGCTATCCAGTATTTACCGGCGGTACGCG
CGATGGCGTTTCGCTTAAAGGAGCACTTGCCTGGATCTATTGATTTTAACGACCTTGTTTCTATCGGCACTGAAGAATTG
ATCAAACTAGCTAGGCGTTATGAGAGTGCATTGAATGACTCTTTTTGGGGGTATGCGAAAACTCGTGTCAATGGGGCGAT
GCTAGATTATTTGCGCTCTTTAGATGTGGTTTCTCGCTCTAGTAGGAAACTCATTAAAAGCATTGATTTTGAAACGACCA
AATACCTTAATGAGCATGGGAAAGAGCCTAGCGATGAGTATTTAGCACAGGCTTTAGGCGAGAATATTGAAAAGATTAGA
GAAGCCAAGACCGCTTCAGATATTTATGCGTTAGTGCCCATAGATGAGCAATTCAATGCGATTGAACAAGATGAAATCAC
TAAAAAGATTGAAGTAGAAGAATTATTAGAGCATGTCCAAAAAGTGTTGAATCAAATGAGCGAGAGGGAGCAAATGCTTA
TCCAGCTTTATTACTTTGAAGAGTTGAATTTGAGCGAGATTAAAGAGATTTTAGGCATTACCGAATCGCGCATTTCTCAA
ATCATCAAAGAAGCGATTAAAAAGGTGCGTCAATCTTTAGGAGCTAGTCATGGCTGA

Upstream 100 bases:

>100_bases
TTTGATAAAAAAGGCACTGAAGAGAGCTTGCTTCGTTTCAACCATGAGTTTAAAAATAGAGAAAAAGAAGTGGCTAGTAT
TTTAAATTATATTAGAAATT

Downstream 100 bases:

>100_bases
TATTTTAAGCCAAGAAGAAATTGATGCACTTTTAGAAGTTGTTGATGAAAATGTGGATATTCAAAATGTCCAAAAAAAGG
ATATTACTCCGCAACGCAGT

Product: flagellar biosynthesis sigma factor

Products: NA

Alternate protein names: Sigma-27; Sigma-28; Sigma-F factor [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MILTMENRMPQEIQQTEPSEKSEKNKEKVLNAYDKQQHHHQDALAIQYLPAVRAMAFRLKEHLPGSIDFNDLVSIGTEEL
IKLARRYESALNDSFWGYAKTRVNGAMLDYLRSLDVVSRSSRKLIKSIDFETTKYLNEHGKEPSDEYLAQALGENIEKIR
EAKTASDIYALVPIDEQFNAIEQDEITKKIEVEELLEHVQKVLNQMSEREQMLIQLYYFEELNLSEIKEILGITESRISQ
IIKEAIKKVRQSLGASHG

Sequences:

>Translated_258_residues
MILTMENRMPQEIQQTEPSEKSEKNKEKVLNAYDKQQHHHQDALAIQYLPAVRAMAFRLKEHLPGSIDFNDLVSIGTEEL
IKLARRYESALNDSFWGYAKTRVNGAMLDYLRSLDVVSRSSRKLIKSIDFETTKYLNEHGKEPSDEYLAQALGENIEKIR
EAKTASDIYALVPIDEQFNAIEQDEITKKIEVEELLEHVQKVLNQMSEREQMLIQLYYFEELNLSEIKEILGITESRISQ
IIKEAIKKVRQSLGASHG
>Mature_258_residues
MILTMENRMPQEIQQTEPSEKSEKNKEKVLNAYDKQQHHHQDALAIQYLPAVRAMAFRLKEHLPGSIDFNDLVSIGTEEL
IKLARRYESALNDSFWGYAKTRVNGAMLDYLRSLDVVSRSSRKLIKSIDFETTKYLNEHGKEPSDEYLAQALGENIEKIR
EAKTASDIYALVPIDEQFNAIEQDEITKKIEVEELLEHVQKVLNQMSEREQMLIQLYYFEELNLSEIKEILGITESRISQ
IIKEAIKKVRQSLGASHG

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is specific for class 3 flagellar operons [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=223, Percent_Identity=33.6322869955157, Blast_Score=114, Evalue=8e-27,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 29826; Mature: 29826

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILTMENRMPQEIQQTEPSEKSEKNKEKVLNAYDKQQHHHQDALAIQYLPAVRAMAFRLK
CEEECCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EHLPGSIDFNDLVSIGTEELIKLARRYESALNDSFWGYAKTRVNGAMLDYLRSLDVVSRS
HHCCCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SRKLIKSIDFETTKYLNEHGKEPSDEYLAQALGENIEKIREAKTASDIYALVPIDEQFNA
HHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHCH
IEQDEITKKIEVEELLEHVQKVLNQMSEREQMLIQLYYFEELNLSEIKEILGITESRISQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHH
IIKEAIKKVRQSLGASHG
HHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MILTMENRMPQEIQQTEPSEKSEKNKEKVLNAYDKQQHHHQDALAIQYLPAVRAMAFRLK
CEEECCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EHLPGSIDFNDLVSIGTEELIKLARRYESALNDSFWGYAKTRVNGAMLDYLRSLDVVSRS
HHCCCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SRKLIKSIDFETTKYLNEHGKEPSDEYLAQALGENIEKIREAKTASDIYALVPIDEQFNA
HHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHCH
IEQDEITKKIEVEELLEHVQKVLNQMSEREQMLIQLYYFEELNLSEIKEILGITESRISQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHH
IIKEAIKKVRQSLGASHG
HHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]