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The map label for this gene is truD

Identifier: 109947624

GI number: 109947624

Start: 943312

End: 944457

Strand: Reverse

Name: truD

Synonym: Hac_1089

Alternate gene names: 109947624

Gene position: 944457-943312 (Counterclockwise)

Preceding gene: 109947625

Following gene: 109947623

Centisome position: 60.78

GC content: 33.07

Gene sequence:

>1146_bases
ATGAATTTAAACTTTATGCCCTTATTGCATGCTTATAACCATGCGAGCATTGATTTTCATTTCAATTCTAGCGCAAGGGA
TTTTTGCGTGCATGAAGTGCCTTTGTATGAATTTAGTAATAAGGGCGAACATGCCATTATTCAAGTGAGAAAAAGCGGTT
TAAGCACTTTAGAAATGCTTCAAATTTTTTCTCAAATTTTAGGGGTAAAAATTGCAGAATTGGGTTATGCGGGCTTGAAA
GATAAAAACGCGCTAACGACTCAATTTGTCTCACTCCCTAAAAAATACGCCCCCTTACTAGAAAAAAATACGAGCAACTT
TCAAGAAAGAAACCTTAAGATTTTATCTTTAAATTACCATCATAATAAGATCAAATTAGGGCATTTAAAGGGGAATCGCT
TTTTTATGCGTTTTAAGAAAATGACCCCCCTAAACGCTCACAAGACAAAGCAGGTTTTAGAACAAATCGTGCAGTTTGGT
ATGCCTAATTATTTTGGTTCACAACGCTTTGGGAAGTTTAATGACAACCATAAAGAGGGTTTAAAAATCTTACAAAATGA
AGCGAAATTTAAGAATCAAAAATTAAACGCTTTTTTAATCTCAAGCTATCAAAGTTACTTGTTTAATTCGCTTTTAAGCA
AACGATTAGAAATCAGTAAAATAATCAGCGACTTTAGCCTAAAAGAGGGTTTAGAGTTTTTTAAACAAAAAAATTTAAAT
ATCCATTCAAACGCCCTAAAAGCCCTTAAAAACCAAGATCACCCCTTTAAAATCCTAGAAGGTGATGTGATGTGCCATTA
CCCTTATGGGAAGTTTTTTGACGCTTTAGAGTTAGAAAAAGAAAGCGAAAGGTTTTTAAAAAAAGAAGCTGTGCCTATGG
GGTTATTAGATGGTAAAAAGGCTCTTTATGCAAAAAATTTGAGTTTAGAAATTGAAAAAGAATTCCAAAACCATATTTTA
AGTGACCATGCTAAAACGCTAGGCTCTAGGCGGTTTTTTTGGGTGTTTGTAGAAAATTTAACTTCCAAATATATAAAAGA
AAAAGTGCAATTTGAATTGGAATTTTACTTGCCTAAAGGGAGTTATGCGAGTGCATTGCTCAAAGAAATCAAGCATCAGA
AAGGAGAAAATAATGATGAATTTTGA

Upstream 100 bases:

>100_bases
TCACTTTCACTAAAATCGCTCAAGGTGTGCCTGCTGGAGTGAATTTTGAAAACATTGACTCAGTCTCCCTTTCAAGGGCG
TTCAATTCAAGGATCAAAGC

Downstream 100 bases:

>100_bases
AAAGATTATCGCACAAAACAAGCTCAAAACGAATGCGGTTTTAACCACTTATTGCGTGATTTTTGCTTTTATTGGGTTGT
TAGTGGATGTTATTAGAATC

Product: tRNA pseudouridine synthase D

Products: NA

Alternate protein names: tRNA pseudouridylate synthase D; tRNA-uridine isomerase D

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MNLNFMPLLHAYNHASIDFHFNSSARDFCVHEVPLYEFSNKGEHAIIQVRKSGLSTLEMLQIFSQILGVKIAELGYAGLK
DKNALTTQFVSLPKKYAPLLEKNTSNFQERNLKILSLNYHHNKIKLGHLKGNRFFMRFKKMTPLNAHKTKQVLEQIVQFG
MPNYFGSQRFGKFNDNHKEGLKILQNEAKFKNQKLNAFLISSYQSYLFNSLLSKRLEISKIISDFSLKEGLEFFKQKNLN
IHSNALKALKNQDHPFKILEGDVMCHYPYGKFFDALELEKESERFLKKEAVPMGLLDGKKALYAKNLSLEIEKEFQNHIL
SDHAKTLGSRRFFWVFVENLTSKYIKEKVQFELEFYLPKGSYASALLKEIKHQKGENNDEF

Sequences:

>Translated_381_residues
MNLNFMPLLHAYNHASIDFHFNSSARDFCVHEVPLYEFSNKGEHAIIQVRKSGLSTLEMLQIFSQILGVKIAELGYAGLK
DKNALTTQFVSLPKKYAPLLEKNTSNFQERNLKILSLNYHHNKIKLGHLKGNRFFMRFKKMTPLNAHKTKQVLEQIVQFG
MPNYFGSQRFGKFNDNHKEGLKILQNEAKFKNQKLNAFLISSYQSYLFNSLLSKRLEISKIISDFSLKEGLEFFKQKNLN
IHSNALKALKNQDHPFKILEGDVMCHYPYGKFFDALELEKESERFLKKEAVPMGLLDGKKALYAKNLSLEIEKEFQNHIL
SDHAKTLGSRRFFWVFVENLTSKYIKEKVQFELEFYLPKGSYASALLKEIKHQKGENNDEF
>Mature_381_residues
MNLNFMPLLHAYNHASIDFHFNSSARDFCVHEVPLYEFSNKGEHAIIQVRKSGLSTLEMLQIFSQILGVKIAELGYAGLK
DKNALTTQFVSLPKKYAPLLEKNTSNFQERNLKILSLNYHHNKIKLGHLKGNRFFMRFKKMTPLNAHKTKQVLEQIVQFG
MPNYFGSQRFGKFNDNHKEGLKILQNEAKFKNQKLNAFLISSYQSYLFNSLLSKRLEISKIISDFSLKEGLEFFKQKNLN
IHSNALKALKNQDHPFKILEGDVMCHYPYGKFFDALELEKESERFLKKEAVPMGLLDGKKALYAKNLSLEIEKEFQNHIL
SDHAKTLGSRRFFWVFVENLTSKYIKEKVQFELEFYLPKGSYASALLKEIKHQKGENNDEF

Specific function: Responsible for synthesis of pseudouridine from uracil- 13 in transfer RNAs

COG id: COG0585

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRUD domain

Homologues:

Organism=Homo sapiens, GI148762985, Length=147, Percent_Identity=31.2925170068027, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI148762961, Length=147, Percent_Identity=31.2925170068027, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI148762948, Length=147, Percent_Identity=31.2925170068027, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1789102, Length=361, Percent_Identity=26.3157894736842, Blast_Score=127, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6324817, Length=178, Percent_Identity=32.5842696629214, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI21355799, Length=125, Percent_Identity=32, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUD_HELAH (Q17WX3)

Other databases:

- EMBL:   AM260522
- RefSeq:   YP_664852.1
- ProteinModelPortal:   Q17WX3
- SMR:   Q17WX3
- STRING:   Q17WX3
- GeneID:   4176990
- GenomeReviews:   AM260522_GR
- KEGG:   hac:Hac_1089
- NMPDR:   fig|382638.8.peg.1058
- eggNOG:   COG0585
- HOGENOM:   HBG541221
- OMA:   FFIRLKK
- PhylomeDB:   Q17WX3
- ProtClustDB:   PRK00984
- BioCyc:   HACI382638:HAC_1089-MONOMER
- HAMAP:   MF_01082
- InterPro:   IPR020103
- InterPro:   IPR001656
- InterPro:   IPR020119
- InterPro:   IPR011760
- PANTHER:   PTHR13326
- TIGRFAMs:   TIGR00094

Pfam domain/function: PF01142 TruD; SSF55120 PsdUridine_synth_cat_dom

EC number: NA

Molecular weight: Translated: 44352; Mature: 44352

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: PS50984 TRUD; PS01268 UPF0024

Important sites: ACT_SITE 81-81

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLNFMPLLHAYNHASIDFHFNSSARDFCVHEVPLYEFSNKGEHAIIQVRKSGLSTLEML
CCCCCCHHHHHCCCCEEEEEECCCCCCCEEECCCHHHCCCCCCEEEEEEEHHCCHHHHHH
QIFSQILGVKIAELGYAGLKDKNALTTQFVSLPKKYAPLLEKNTSNFQERNLKILSLNYH
HHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHCCHHHHHHHHCCCCCHHHCCEEEEEEEEC
HNKIKLGHLKGNRFFMRFKKMTPLNAHKTKQVLEQIVQFGMPNYFGSQRFGKFNDNHKEG
CCEEEEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHH
LKILQNEAKFKNQKLNAFLISSYQSYLFNSLLSKRLEISKIISDFSLKEGLEFFKQKNLN
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
IHSNALKALKNQDHPFKILEGDVMCHYPYGKFFDALELEKESERFLKKEAVPMGLLDGKK
CHHHHHHHHHCCCCCEEEEECCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
ALYAKNLSLEIEKEFQNHILSDHAKTLGSRRFFWVFVENLTSKYIKEKVQFELEFYLPKG
HHEECCCCEEEHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHEEEEEEEECCC
SYASALLKEIKHQKGENNDEF
HHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNLNFMPLLHAYNHASIDFHFNSSARDFCVHEVPLYEFSNKGEHAIIQVRKSGLSTLEML
CCCCCCHHHHHCCCCEEEEEECCCCCCCEEECCCHHHCCCCCCEEEEEEEHHCCHHHHHH
QIFSQILGVKIAELGYAGLKDKNALTTQFVSLPKKYAPLLEKNTSNFQERNLKILSLNYH
HHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHCCHHHHHHHHCCCCCHHHCCEEEEEEEEC
HNKIKLGHLKGNRFFMRFKKMTPLNAHKTKQVLEQIVQFGMPNYFGSQRFGKFNDNHKEG
CCEEEEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHH
LKILQNEAKFKNQKLNAFLISSYQSYLFNSLLSKRLEISKIISDFSLKEGLEFFKQKNLN
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
IHSNALKALKNQDHPFKILEGDVMCHYPYGKFFDALELEKESERFLKKEAVPMGLLDGKK
CHHHHHHHHHCCCCCEEEEECCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
ALYAKNLSLEIEKEFQNHILSDHAKTLGSRRFFWVFVENLTSKYIKEKVQFELEFYLPKG
HHEECCCCEEEHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHEEEEEEEECCC
SYASALLKEIKHQKGENNDEF
HHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA