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The map label for this gene is tilS [H]

Identifier: 109947271

GI number: 109947271

Start: 606620

End: 607669

Strand: Direct

Name: tilS [H]

Synonym: Hac_0697

Alternate gene names: 109947271

Gene position: 606620-607669 (Clockwise)

Preceding gene: 109947270

Following gene: 109947273

Centisome position: 39.04

GC content: 33.24

Gene sequence:

>1050_bases
ATGCGATCAATTAAGCATACATTGAGTGTAAATTTTATAGTGCAAGATTTTAAAGCTTATTTAGAGCCCTTAAGAGAGGG
TAAAAATTTATTGGGTTTTTCAGGTGGGGTGGATTCTACTTGCTTGTTTCATCTTTTAGTTAAAGAAAATATCGCCTTTG
ACATCGCGTTAGTGGATTATAACACGCAAAAACAACGCCTTGAAATCATCAAACACGCTCAAAAACTCGCAAAAACACAC
CATAAAAAATGCTATATCCATTACGCTCAAAAAATCACGCATGATTTTGAAATGCAAGCGAGAAAAATCCGTTATGATTT
TTTTGAAACCCTAATCAGAGAGCATTCTTACAAGCATTTGATTTTAGCGCACCACTTGAATGACAGGCTAGAATGGTTTT
TGATGCAATTAAGCAAGGGAGCTGGGTTGAACACGCTTTTAAGCTTTCAAGCTTATGAAAAAAGAGGGTTTTATGCGATT
GTGCGTCCCTTACTCTACACCTCTAAAGACATTCTTAAAACGCTCGCTAAAGATATGAAATTTTTTGAAGACTTTTCTAA
TTATTCTTTGAAATTCAAACGCAATTTTTTTAGAAAACATTACGCCAATTCCTTAATGCAATATTATTCCAAAGGCATTA
TCCAAAGTTTTAAATTTTTGGATCAAGAAAAAGAGCGGCTTTACCCTTTGACCCCCTTTTCACAAATGCATGGGATCATT
TTTTTTAAATATTCACAAAACGCGCTTTTTATAGTGGATAAAATCTTAAAACAAAAGGGGTATGTGTTGAGTTTTTCTCA
AAAAGAAGAAATCAAACGCCATTTTTTTAGTTTAGAAATCGCTCAAAAATTCATCATTGAAAAGGATAAAGAGCATGTCT
TTATCGCCCTTAAACCCCAACAAACTTTAAGCATGCCAAAGGATTTTAAAGACAAAGCTAGGAGATTGAATATCCCTAAA
CGCTTACGGTCTATTTTATACACAGAGTTTTTAAAACAACCAACGCATGATTTTTTAATCCGTTTTAAACAGAGTTTAGA
AAAGATTTAA

Upstream 100 bases:

>100_bases
AGTTTGAATGAAGTTGAAAAAGTAAGGAATGAAATTTTAAGCATAGAGGGTGTGAGCTTACTAAAAAGCATTTTAACCCA
TAAGGAAATTTCATGAAAGA

Downstream 100 bases:

>100_bases
AGCTTTGTTTTATGAGCGTTGGTTATCAAAAATACTAAAAGCGTTCTTTAAATAAACATCAAAGAGCTTGCGTGAGCGAA
TGATAGCTTTTTAAAAGCGC

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MRSIKHTLSVNFIVQDFKAYLEPLREGKNLLGFSGGVDSTCLFHLLVKENIAFDIALVDYNTQKQRLEIIKHAQKLAKTH
HKKCYIHYAQKITHDFEMQARKIRYDFFETLIREHSYKHLILAHHLNDRLEWFLMQLSKGAGLNTLLSFQAYEKRGFYAI
VRPLLYTSKDILKTLAKDMKFFEDFSNYSLKFKRNFFRKHYANSLMQYYSKGIIQSFKFLDQEKERLYPLTPFSQMHGII
FFKYSQNALFIVDKILKQKGYVLSFSQKEEIKRHFFSLEIAQKFIIEKDKEHVFIALKPQQTLSMPKDFKDKARRLNIPK
RLRSILYTEFLKQPTHDFLIRFKQSLEKI

Sequences:

>Translated_349_residues
MRSIKHTLSVNFIVQDFKAYLEPLREGKNLLGFSGGVDSTCLFHLLVKENIAFDIALVDYNTQKQRLEIIKHAQKLAKTH
HKKCYIHYAQKITHDFEMQARKIRYDFFETLIREHSYKHLILAHHLNDRLEWFLMQLSKGAGLNTLLSFQAYEKRGFYAI
VRPLLYTSKDILKTLAKDMKFFEDFSNYSLKFKRNFFRKHYANSLMQYYSKGIIQSFKFLDQEKERLYPLTPFSQMHGII
FFKYSQNALFIVDKILKQKGYVLSFSQKEEIKRHFFSLEIAQKFIIEKDKEHVFIALKPQQTLSMPKDFKDKARRLNIPK
RLRSILYTEFLKQPTHDFLIRFKQSLEKI
>Mature_349_residues
MRSIKHTLSVNFIVQDFKAYLEPLREGKNLLGFSGGVDSTCLFHLLVKENIAFDIALVDYNTQKQRLEIIKHAQKLAKTH
HKKCYIHYAQKITHDFEMQARKIRYDFFETLIREHSYKHLILAHHLNDRLEWFLMQLSKGAGLNTLLSFQAYEKRGFYAI
VRPLLYTSKDILKTLAKDMKFFEDFSNYSLKFKRNFFRKHYANSLMQYYSKGIIQSFKFLDQEKERLYPLTPFSQMHGII
FFKYSQNALFIVDKILKQKGYVLSFSQKEEIKRHFFSLEIAQKFIIEKDKEHVFIALKPQQTLSMPKDFKDKARRLNIPK
RLRSILYTEFLKQPTHDFLIRFKQSLEKI

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=190, Percent_Identity=29.4736842105263, Blast_Score=76, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 41827; Mature: 41827

Theoretical pI: Translated: 10.35; Mature: 10.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure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HHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]