Click here to switch to the map view.

The map label for this gene is BSP6IM [H]

Identifier: 109947180

GI number: 109947180

Start: 521790

End: 522737

Strand: Direct

Name: BSP6IM [H]

Synonym: Hac_0595

Alternate gene names: 109947180

Gene position: 521790-522737 (Clockwise)

Preceding gene: 109947179

Following gene: 109947181

Centisome position: 33.58

GC content: 35.23

Gene sequence:

>948_bases
ATGGATTTTTGCTCTGGTATTGGCGGAGGCCGTTTGGGCTTGGAGCGGTGCCATTTAAAATGCGTAGGGCATGCAGAAAT
CAACCATGAAGCCCTTAGAACCTATGAATTATTTTTTAAAGATACTCATAATTTTGGGGATTTGATGCGAATCAACCCTA
ATGATTTACCCGATTTTGATGTCTTAATTAGCGGGTTTCCTTGTCAAGCTTTTTCTATCAATGGTAAAAGGAAGGGGCTT
GATGATGAAAGGGGGACGATTATTTATGGGCTTGTTCATATTTTAAAAGTTAAGCAACCCAAATGTTTCTTGCTTGAAAA
TGTTAAGGGGTTAATCAATCATAGGCAACAAGAAACTTTTGAAATCATCATCAAAGCCTTGCAAGAAGTGGGTTATACAA
CTTATTATCAAATTTTGAATAGCGCTGATTTTCAATTAGCTCAAAAGAGGGAGCGCCTTTATATCGTGGGGTTTAGAAAG
GATTTAAAACACCAATTTAATTTTCCTTTGGGTTTAGCCAATGATTATTGTTTTAAGGATTTTTTAGACGCTGATAATGA
ATGCTATTTGAATACCAATAACGCTGCATTTCAAAGATACTTGCACAACTCATACAATCATAACCGGGTTTTTTTAGAGA
ATATTTTAACTTTAGAAAACGCTGTTTTAGATACAAGACAATCTGATTTAAGATTGTATTTTAATGTTTTTCCTACTTTA
AGGACTTCTCGGCATGGCTTGTTTTATATCCAAAAAGGAAAAATCAAAAGATTAAACGCTGTTGAAAGCCTGCTTTTGCA
AGGATTTCCTAGAGATTTGATCGCTAAGATTAAAGATAATCCTAACTTTAAAGGAAGCCATTTATTATCCCAAGCAGGGA
ACGCCATGAGCGTGAATGTGATTTTTGCTCTCGCTAAACAAATGTTAAGAGCGATAGTTGAGCGTTAA

Upstream 100 bases:

>100_bases
AATTTAGTTTCTTTGTGTTTTAAAGCGTGTTTGCCATTCTTTTAATGGAGCGTGTTTTTCTTTGCGTGTTAGGGATAATT
GATTGGGAATTTTAACTTTT

Downstream 100 bases:

>100_bases
TCATCAAATGTAAGATAAAAAATTAAAGCAAGAAATAAAAGTTTTTCTTAAATAAGGTTTTAACGATCAATATTTGAAAA
AAACAAAGCCCTAATAAAAT

Product: C-5 cytosine-specific DNA methylase

Products: NA

Alternate protein names: M.NgoBI; Cytosine-specific methyltransferase NgoBI; M.NgoI [H]

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MDFCSGIGGGRLGLERCHLKCVGHAEINHEALRTYELFFKDTHNFGDLMRINPNDLPDFDVLISGFPCQAFSINGKRKGL
DDERGTIIYGLVHILKVKQPKCFLLENVKGLINHRQQETFEIIIKALQEVGYTTYYQILNSADFQLAQKRERLYIVGFRK
DLKHQFNFPLGLANDYCFKDFLDADNECYLNTNNAAFQRYLHNSYNHNRVFLENILTLENAVLDTRQSDLRLYFNVFPTL
RTSRHGLFYIQKGKIKRLNAVESLLLQGFPRDLIAKIKDNPNFKGSHLLSQAGNAMSVNVIFALAKQMLRAIVER

Sequences:

>Translated_315_residues
MDFCSGIGGGRLGLERCHLKCVGHAEINHEALRTYELFFKDTHNFGDLMRINPNDLPDFDVLISGFPCQAFSINGKRKGL
DDERGTIIYGLVHILKVKQPKCFLLENVKGLINHRQQETFEIIIKALQEVGYTTYYQILNSADFQLAQKRERLYIVGFRK
DLKHQFNFPLGLANDYCFKDFLDADNECYLNTNNAAFQRYLHNSYNHNRVFLENILTLENAVLDTRQSDLRLYFNVFPTL
RTSRHGLFYIQKGKIKRLNAVESLLLQGFPRDLIAKIKDNPNFKGSHLLSQAGNAMSVNVIFALAKQMLRAIVER
>Mature_315_residues
MDFCSGIGGGRLGLERCHLKCVGHAEINHEALRTYELFFKDTHNFGDLMRINPNDLPDFDVLISGFPCQAFSINGKRKGL
DDERGTIIYGLVHILKVKQPKCFLLENVKGLINHRQQETFEIIIKALQEVGYTTYYQILNSADFQLAQKRERLYIVGFRK
DLKHQFNFPLGLANDYCFKDFLDADNECYLNTNNAAFQRYLHNSYNHNRVFLENILTLENAVLDTRQSDLRLYFNVFPTL
RTSRHGLFYIQKGKIKRLNAVESLLLQGFPRDLIAKIKDNPNFKGSHLLSQAGNAMSVNVIFALAKQMLRAIVER

Specific function: This methylase recognizes the double-stranded sequence RGCGCY, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the NgoBI endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788271, Length=208, Percent_Identity=34.6153846153846, Blast_Score=106, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 36315; Mature: 36315

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: PS00094 C5_MTASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFCSGIGGGRLGLERCHLKCVGHAEINHEALRTYELFFKDTHNFGDLMRINPNDLPDFD
CCCCCCCCCCCCCHHHHEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHH
VLISGFPCQAFSINGKRKGLDDERGTIIYGLVHILKVKQPKCFLLENVKGLINHRQQETF
HEEECCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
EIIIKALQEVGYTTYYQILNSADFQLAQKRERLYIVGFRKDLKHQFNFPLGLANDYCFKD
HHHHHHHHHCCCHHHHHHHCCCHHHHHHCCCCEEEEEEHHHHHHHCCCCCCCCCCHHHHH
FLDADNECYLNTNNAAFQRYLHNSYNHNRVFLENILTLENAVLDTRQSDLRLYFNVFPTL
HHCCCCEEEEECCHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHCCCCCEEEEEEECCCC
RTSRHGLFYIQKGKIKRLNAVESLLLQGFPRDLIAKIKDNPNFKGSHLLSQAGNAMSVNV
CCCCCCEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHCCCEEEHHH
IFALAKQMLRAIVER
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MDFCSGIGGGRLGLERCHLKCVGHAEINHEALRTYELFFKDTHNFGDLMRINPNDLPDFD
CCCCCCCCCCCCCHHHHEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHH
VLISGFPCQAFSINGKRKGLDDERGTIIYGLVHILKVKQPKCFLLENVKGLINHRQQETF
HEEECCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
EIIIKALQEVGYTTYYQILNSADFQLAQKRERLYIVGFRKDLKHQFNFPLGLANDYCFKD
HHHHHHHHHCCCHHHHHHHCCCHHHHHHCCCCEEEEEEHHHHHHHCCCCCCCCCCHHHHH
FLDADNECYLNTNNAAFQRYLHNSYNHNRVFLENILTLENAVLDTRQSDLRLYFNVFPTL
HHCCCCEEEEECCHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHCCCCCEEEEEEECCCC
RTSRHGLFYIQKGKIKRLNAVESLLLQGFPRDLIAKIKDNPNFKGSHLLSQAGNAMSVNV
CCCCCCEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHCCCEEEHHH
IFALAKQMLRAIVER
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9628358 [H]