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The map label for this gene is hpcH [H]

Identifier: 108812517

GI number: 108812517

Start: 2639325

End: 2640131

Strand: Reverse

Name: hpcH [H]

Synonym: YPN_2356

Alternate gene names: 108812517

Gene position: 2640131-2639325 (Counterclockwise)

Preceding gene: 108812518

Following gene: 108812516

Centisome position: 58.22

GC content: 51.3

Gene sequence:

>807_bases
ATGTTTCCATTAACAAATAAATTTAAACAGGCACTGAAAGCAAAAAAACCACAAATTGGCTTATGGCTAGGTTTGTGTAG
CAATTACAGTGCTGAAATCCTGGCCGGTGCGGGGTTTGACTGGTTGTTGATCGACGATGAACATGCCCCGAATGATGTAC
AACGGATCTTAGGGCAATTACAGACGATCGCGTCTTATCCGAGCCAGCCCGTGGTTCGTCCGGCGTGGAATGACCCGGTA
ATGATAAAAAAATTGCTGGATATTGGCGCACAAACCTTATTGCTCCCAATGATTCAAAACGCCGCACAAGCGCGCGCTGC
GATCAGTGCGACCCGCTATCCTCCGCAGGGGATTCGCGGCGTCGGAAGCGCGCTGGCCCGAGCTTCCCGTTGGAATAGAA
TCGCAGATTATGTGCAGCAAGCTGACGAACAGATATGCGTATTGCTCCAAATAGAAACACGCGAAGCACTGCGCAATCTA
CCCGAGATTTTGGCGGTTGATGGTGTCGATGGTGTGTTTATCGGCCCGGCCGATCTCGCTTCTGATATGGGTTTTGCGGG
TAATCCACACCATCCCGAGGTCCGTCAAGCCATCGATAGCGCAGTGATGAAAATTAAGACTGCCGATAAAGCGGCCGGGA
TCTTGATGACCACGCCAGAGTTGGCTGAGTATTATCTGAAACTGGGGGCACTATTTGTGGCTGTAGGTGTAGATACGACG
CTATTAGCACGCACAGCCGAGGCACTGGCAGCCCGCTTCGGTGTGGGTAATCATCATCCACCGCTTGCACCATCGGGTGG
ATATTAA

Upstream 100 bases:

>100_bases
TCCTTCACCCGCCCGGTTCCAGCGCGTCGTGGTGATACTTTCCATGTGGATTATGGCCCGTTAGGTGCCATAAGTTGCCA
CTTTGTTTAGGGGCTTAGTG

Downstream 100 bases:

>100_bases
CCCTGTCCGTATCGAGGACATTATCATGAGCGATTCACTGTCTACCGCACCGGGCGTTACCCCGCCGGTCAATAAAAACA
CACCATTAAGTGCGCAGCAG

Product: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Products: NA

Alternate protein names: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase; HHED aldolase; 4-hydroxy-2-ketoheptane-1,7-dioate aldolase; HKHD aldolase [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MFPLTNKFKQALKAKKPQIGLWLGLCSNYSAEILAGAGFDWLLIDDEHAPNDVQRILGQLQTIASYPSQPVVRPAWNDPV
MIKKLLDIGAQTLLLPMIQNAAQARAAISATRYPPQGIRGVGSALARASRWNRIADYVQQADEQICVLLQIETREALRNL
PEILAVDGVDGVFIGPADLASDMGFAGNPHHPEVRQAIDSAVMKIKTADKAAGILMTTPELAEYYLKLGALFVAVGVDTT
LLARTAEALAARFGVGNHHPPLAPSGGY

Sequences:

>Translated_268_residues
MFPLTNKFKQALKAKKPQIGLWLGLCSNYSAEILAGAGFDWLLIDDEHAPNDVQRILGQLQTIASYPSQPVVRPAWNDPV
MIKKLLDIGAQTLLLPMIQNAAQARAAISATRYPPQGIRGVGSALARASRWNRIADYVQQADEQICVLLQIETREALRNL
PEILAVDGVDGVFIGPADLASDMGFAGNPHHPEVRQAIDSAVMKIKTADKAAGILMTTPELAEYYLKLGALFVAVGVDTT
LLARTAEALAARFGVGNHHPPLAPSGGY
>Mature_268_residues
MFPLTNKFKQALKAKKPQIGLWLGLCSNYSAEILAGAGFDWLLIDDEHAPNDVQRILGQLQTIASYPSQPVVRPAWNDPV
MIKKLLDIGAQTLLLPMIQNAAQARAAISATRYPPQGIRGVGSALARASRWNRIADYVQQADEQICVLLQIETREALRNL
PEILAVDGVDGVFIGPADLASDMGFAGNPHHPEVRQAIDSAVMKIKTADKAAGILMTTPELAEYYLKLGALFVAVGVDTT
LLARTAEALAARFGVGNHHPPLAPSGGY

Specific function: Catalyzes the reversible retro-aldol cleavage of 4- hydroxy-2-ketoheptane-1,7-dioate (HKHD) to pyruvate and succinic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788578, Length=262, Percent_Identity=51.1450381679389, Blast_Score=278, Evalue=2e-76,
Organism=Escherichia coli, GI1789514, Length=253, Percent_Identity=44.6640316205534, Blast_Score=222, Evalue=3e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR012689
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.2.-

Molecular weight: Translated: 28854; Mature: 28854

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFPLTNKFKQALKAKKPQIGLWLGLCSNYSAEILAGAGFDWLLIDDEHAPNDVQRILGQL
CCCCCHHHHHHHHHCCCCCEEEEEHHCCCCCCEEECCCCEEEEECCCCCCHHHHHHHHHH
QTIASYPSQPVVRPAWNDPVMIKKLLDIGAQTLLLPMIQNAAQARAAISATRYPPQGIRG
HHHHHCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
VGSALARASRWNRIADYVQQADEQICVLLQIETREALRNLPEILAVDGVDGVFIGPADLA
HHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECHHHHHHCCHHHEEECCCCCEEECHHHHH
SDMGFAGNPHHPEVRQAIDSAVMKIKTADKAAGILMTTPELAEYYLKLGALFVAVGVDTT
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHCCCHH
LLARTAEALAARFGVGNHHPPLAPSGGY
HHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFPLTNKFKQALKAKKPQIGLWLGLCSNYSAEILAGAGFDWLLIDDEHAPNDVQRILGQL
CCCCCHHHHHHHHHCCCCCEEEEEHHCCCCCCEEECCCCEEEEECCCCCCHHHHHHHHHH
QTIASYPSQPVVRPAWNDPVMIKKLLDIGAQTLLLPMIQNAAQARAAISATRYPPQGIRG
HHHHHCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
VGSALARASRWNRIADYVQQADEQICVLLQIETREALRNLPEILAVDGVDGVFIGPADLA
HHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECHHHHHHCCHHHEEECCCCCEEECHHHHH
SDMGFAGNPHHPEVRQAIDSAVMKIKTADKAAGILMTTPELAEYYLKLGALFVAVGVDTT
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHCCCHH
LLARTAEALAARFGVGNHHPPLAPSGGY
HHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA