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The map label for this gene is hpaC [H]

Identifier: 108812514

GI number: 108812514

Start: 2635784

End: 2636281

Strand: Reverse

Name: hpaC [H]

Synonym: YPN_2353

Alternate gene names: 108812514

Gene position: 2636281-2635784 (Counterclockwise)

Preceding gene: 108812515

Following gene: 108812510

Centisome position: 58.14

GC content: 46.79

Gene sequence:

>498_bases
ATGTCAACAGAAAATCAACATCATCTGTGTTTTCGTGACGCGATGGCCTGTTTATCGGCAGCGGTTAATATTGTGACCAC
CGATGGCCCTGCCGGACGTTGTGGCATTACAGCAACCGCAGTGTGTTCCGTGACAGATACCCCCCCGACCTTGATAATTT
GTATTAATCGTAATAGTGCAATGAACCCTGTTTTTCAGCAAAATGGATGTTTATGCATCAACGTACTGAATCATCAACAA
GAGCCTATGGCGCGTCATTTTGCTGGAATGACGGGGAGCAGCATGGAAGAACGTTTCAGTTGGGATATCTGGAATGTCGG
TCTGCTCGGCCAGCCGTTGTTACGAGATACCTTAGCCAGTTTGGAGGGGGTGATTGAACAGGTACAAACGATAGGTACAC
ATCTGGTTTATTTGGTACACATCACACAGATCGTTCTGCGTGAGCAAGGCCATGGTCTGATTTACTTTAAACGCCATTTT
CACCCGGTGATGGTGTAA

Upstream 100 bases:

>100_bases
ATTATGATACTCAGGGCTGGACGGTTCCTCATCTGCACAATAATAACGATATTAATCAACTGAATAGGTTAATGAAATAA
TCAGGTACCAGGAGGTTTAT

Downstream 100 bases:

>100_bases
ACCTCTAATGCCCGTCATATTTAACTGACGTGCATTAGAGGTTGTTGACAAAGTGCCTGCGGCGGAGAGAACAGGCAGAG
CGTAAAGAGGCCAGCTTCCC

Product: 4-hydroxyphenylacetate 3-monooxygenase coupling protein

Products: 3,4-dihydroxyphenylacetate; NAD+; H2O

Alternate protein names: 4-HPA 3-monooxygenase small component; Flavin:NADH reductase [H]

Number of amino acids: Translated: 165; Mature: 164

Protein sequence:

>165_residues
MSTENQHHLCFRDAMACLSAAVNIVTTDGPAGRCGITATAVCSVTDTPPTLIICINRNSAMNPVFQQNGCLCINVLNHQQ
EPMARHFAGMTGSSMEERFSWDIWNVGLLGQPLLRDTLASLEGVIEQVQTIGTHLVYLVHITQIVLREQGHGLIYFKRHF
HPVMV

Sequences:

>Translated_165_residues
MSTENQHHLCFRDAMACLSAAVNIVTTDGPAGRCGITATAVCSVTDTPPTLIICINRNSAMNPVFQQNGCLCINVLNHQQ
EPMARHFAGMTGSSMEERFSWDIWNVGLLGQPLLRDTLASLEGVIEQVQTIGTHLVYLVHITQIVLREQGHGLIYFKRHF
HPVMV
>Mature_164_residues
STENQHHLCFRDAMACLSAAVNIVTTDGPAGRCGITATAVCSVTDTPPTLIICINRNSAMNPVFQQNGCLCINVLNHQQE
PMARHFAGMTGSSMEERFSWDIWNVGLLGQPLLRDTLASLEGVIEQVQTIGTHLVYLVHITQIVLREQGHGLIYFKRHFH
PVMV

Specific function: Catalyzes the reduction of free flavins (FMN, FAD and riboflavin) by NADH. Subsequently, the reduced flavins diffuse to the large hpaB component or to other electron acceptors such as cytochrome c and Fe(3+) ion [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family. HpaC subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081819, Length=154, Percent_Identity=48.7012987012987, Blast_Score=157, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002563
- InterPro:   IPR011982
- InterPro:   IPR012349
- InterPro:   IPR009002 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: 1.14.13.3

Molecular weight: Translated: 18240; Mature: 18109

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.2 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
8.5 %Cys+Met (Translated Protein)
4.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
7.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTENQHHLCFRDAMACLSAAVNIVTTDGPAGRCGITATAVCSVTDTPPTLIICINRNSA
CCCCCCCHHHHHHHHHHHHHHHEEEECCCCCCCCCCEEEEEEEECCCCCEEEEEECCCCC
MNPVFQQNGCLCINVLNHQQEPMARHFAGMTGSSMEERFSWDIWNVGLLGQPLLRDTLAS
CCCHHHCCCCEEEEECCCCCCHHHHHHCCCCCCCHHHHHCCCEEECCCCCCHHHHHHHHH
LEGVIEQVQTIGTHLVYLVHITQIVLREQGHGLIYFKRHFHPVMV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCC
>Mature Secondary Structure 
STENQHHLCFRDAMACLSAAVNIVTTDGPAGRCGITATAVCSVTDTPPTLIICINRNSA
CCCCCCHHHHHHHHHHHHHHHEEEECCCCCCCCCCEEEEEEEECCCCCEEEEEECCCCC
MNPVFQQNGCLCINVLNHQQEPMARHFAGMTGSSMEERFSWDIWNVGLLGQPLLRDTLAS
CCCHHHCCCCEEEEECCCCCCHHHHHHCCCCCCCHHHHHCCCEEECCCCCCHHHHHHHHH
LEGVIEQVQTIGTHLVYLVHITQIVLREQGHGLIYFKRHFHPVMV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-hydroxyphenylacetate; NADH; H+; O2

Specific reaction: 4-hydroxyphenylacetate + NADH + H+ + O2 = 3,4-dihydroxyphenylacetate + NAD+ + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8077235; 8550403; 10633095 [H]