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The map label for this gene is mepA

Identifier: 108812356

GI number: 108812356

Start: 2479798

End: 2480586

Strand: Reverse

Name: mepA

Synonym: YPN_2194

Alternate gene names: 108812356

Gene position: 2480586-2479798 (Counterclockwise)

Preceding gene: 108812357

Following gene: 108812355

Centisome position: 54.7

GC content: 53.49

Gene sequence:

>789_bases
ATGGTCCCTGTCATGGCGGCCACGCCATGGCAAAAGATTACGCACCCGGTGGCGGGTTCTCCACAGTCAATCGGTGGCTT
TGCTAATGGTTGCGTGATTGGCGCGCAGCCTTTGCCGCTCGAGTCTGCAGACTATCAAGTGATGCGTTCGGATCAGCGCC
GTTATTTTGGTCACCCTGATTTACTGAATTTTATTCACCGTCTCAGTGCACAAGCTCATCAACAACAATTGGGCACGGTG
TTGATTGGTGATATGGCGATGCCTGCAGGCGGGCGTTTTAGCAGTGGGCATGCCAGCCACCAGTCTGGTTTGGATGTGGA
TATTTGGCTACAACTGCCAAAGCAGCGTTGGAGCCAGCAGCAGTTATTGAAACCGCAGCCGATAGACTTGGTCGCGGTTG
ATGGCAAGCGGGTGATCCCCGCACTTTGGCAGCCACAAATTGAGAGTTTGATCAAATTGGCCGCCAAAGATAACGATGTC
ACGCGTATCTTCGTCAATCCGGCAATTAAAAAGCAGCTTTGTCTGGATGCAGGTGCCGATCGTCAATGGCTGCATAAAGT
CCGGCCGTGGTTTGCTCATCGTGCACACATGCATGTGCGCTTACGTTGTCCGGCCAATAGCCTAGAGTGTGTGGACCAAG
ACACTCCACCGCCGGGCGATGGCTGTGGTGCTGAGCTGGAAAGCTGGTTCCAACCGCCTCCGCCAAGTGCTAAACCAGGT
AAAACCTTGCCACCGCCATTACCGCCTTCTTGTCAGGCTTTACTGGATAACCACTTCGCTACGGAATAA

Upstream 100 bases:

>100_bases
TACTGCGCCAGCGCGCGCAGTGCGGCGATGTGGCTTCAGACGTTCCGCGCTGGTAGATAGCATGAAAAATTGGATAGTGG
GTATGGTGGCATTGGTCACC

Downstream 100 bases:

>100_bases
GTCATGGATTGGTTTACCCTTGGTCCTGCCGTTTTGGGGATACTCTTTGCCGTTGCATTATTAGCCGGGTTCATTGATTC
CATTGCCGGCGGGGGCGGCT

Product: penicillin-insensitive murein endopeptidase

Products: Split Peptidoglycan Strands [C]

Alternate protein names: D-alanyl-D-alanine-endopeptidase; DD-endopeptidase

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MVPVMAATPWQKITHPVAGSPQSIGGFANGCVIGAQPLPLESADYQVMRSDQRRYFGHPDLLNFIHRLSAQAHQQQLGTV
LIGDMAMPAGGRFSSGHASHQSGLDVDIWLQLPKQRWSQQQLLKPQPIDLVAVDGKRVIPALWQPQIESLIKLAAKDNDV
TRIFVNPAIKKQLCLDAGADRQWLHKVRPWFAHRAHMHVRLRCPANSLECVDQDTPPPGDGCGAELESWFQPPPPSAKPG
KTLPPPLPPSCQALLDNHFATE

Sequences:

>Translated_262_residues
MVPVMAATPWQKITHPVAGSPQSIGGFANGCVIGAQPLPLESADYQVMRSDQRRYFGHPDLLNFIHRLSAQAHQQQLGTV
LIGDMAMPAGGRFSSGHASHQSGLDVDIWLQLPKQRWSQQQLLKPQPIDLVAVDGKRVIPALWQPQIESLIKLAAKDNDV
TRIFVNPAIKKQLCLDAGADRQWLHKVRPWFAHRAHMHVRLRCPANSLECVDQDTPPPGDGCGAELESWFQPPPPSAKPG
KTLPPPLPPSCQALLDNHFATE
>Mature_262_residues
MVPVMAATPWQKITHPVAGSPQSIGGFANGCVIGAQPLPLESADYQVMRSDQRRYFGHPDLLNFIHRLSAQAHQQQLGTV
LIGDMAMPAGGRFSSGHASHQSGLDVDIWLQLPKQRWSQQQLLKPQPIDLVAVDGKRVIPALWQPQIESLIKLAAKDNDV
TRIFVNPAIKKQLCLDAGADRQWLHKVRPWFAHRAHMHVRLRCPANSLECVDQDTPPPGDGCGAELESWFQPPPPSAKPG
KTLPPPLPPSCQALLDNHFATE

Specific function: Involved in the removal of murein from the sacculus. May also facilitate integration of nascent murein strands into the sacculus by cleaving the peptide bonds between neighboring strands in mature murein

COG id: COG3770

COG function: function code M; Murein endopeptidase

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M74 family

Homologues:

Organism=Escherichia coli, GI1788668, Length=252, Percent_Identity=73.4126984126984, Blast_Score=391, Evalue=1e-110,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MEPA_YERPA (Q1C665)

Other databases:

- EMBL:   CP000308
- RefSeq:   YP_652002.1
- ProteinModelPortal:   Q1C665
- SMR:   Q1C665
- STRING:   Q1C665
- GeneID:   4122003
- GenomeReviews:   CP000308_GR
- KEGG:   ypa:YPA_2091
- eggNOG:   COG3770
- HOGENOM:   HBG296858
- ProtClustDB:   PRK09429
- BioCyc:   YPES360102:YPA_2091-MONOMER
- GO:   GO:0006508
- HAMAP:   MF_01623
- InterPro:   IPR009045
- InterPro:   IPR005073
- Gene3D:   G3DSA:3.30.1380.10
- PIRSF:   PIRSF018455

Pfam domain/function: PF03411 Peptidase_M74; SSF55166 Hedgehog_sig_N

EC number: 3.4.99.- [C]

Molecular weight: Translated: 28849; Mature: 28849

Theoretical pI: Translated: 8.17; Mature: 8.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPVMAATPWQKITHPVAGSPQSIGGFANGCVIGAQPLPLESADYQVMRSDQRRYFGHPD
CCCCCCCCCHHHHCCCCCCCCHHCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCHH
LLNFIHRLSAQAHQQQLGTVLIGDMAMPAGGRFSSGHASHQSGLDVDIWLQLPKQRWSQQ
HHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHCCHH
QLLKPQPIDLVAVDGKRVIPALWQPQIESLIKLAAKDNDVTRIFVNPAIKKQLCLDAGAD
HCCCCCCCEEEEECCCCCCHHHCCHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHCCCCC
RQWLHKVRPWFAHRAHMHVRLRCPANSLECVDQDTPPPGDGCGAELESWFQPPPPSAKPG
HHHHHHHHHHHHHCCEEEEEEECCCCCHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCCC
KTLPPPLPPSCQALLDNHFATE
CCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MVPVMAATPWQKITHPVAGSPQSIGGFANGCVIGAQPLPLESADYQVMRSDQRRYFGHPD
CCCCCCCCCHHHHCCCCCCCCHHCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCHH
LLNFIHRLSAQAHQQQLGTVLIGDMAMPAGGRFSSGHASHQSGLDVDIWLQLPKQRWSQQ
HHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHCCHH
QLLKPQPIDLVAVDGKRVIPALWQPQIESLIKLAAKDNDVTRIFVNPAIKKQLCLDAGAD
HCCCCCCCEEEEECCCCCCHHHCCHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHCCCCC
RQWLHKVRPWFAHRAHMHVRLRCPANSLECVDQDTPPPGDGCGAELESWFQPPPPSAKPG
HHHHHHHHHHHHHCCEEEEEEECCCCCHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCCC
KTLPPPLPPSCQALLDNHFATE
CCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond [C]

Specific reaction: Split The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond Connecting Neighboring Peptidoglycan Strands. [C]

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA