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The map label for this gene is rlmF [H]

Identifier: 108812276

GI number: 108812276

Start: 2390712

End: 2391722

Strand: Reverse

Name: rlmF [H]

Synonym: YPN_2114

Alternate gene names: 108812276

Gene position: 2391722-2390712 (Counterclockwise)

Preceding gene: 108812278

Following gene: 108812272

Centisome position: 52.74

GC content: 49.36

Gene sequence:

>1011_bases
ATGCTATCTTACGCGCCAGAAAATGCCTATCAGAGAGCCAGTACGATGGAAAATAAAAAAGTATTCCCCAAAGAAAAAAG
CGGATTGCATCCCCGAAATCGTCATTGCTCACGCTATGATTTTGATGCGCTTTCAGTGAGTTGCCCTGAGCTAATCCCTT
TTCTGGCCCCGACCGCCTATGGTGATATTTCTGTCGATTTTGCTGATCCATTAGCAGTAAAAATGTTGAATAAGGCATTA
TTAAAACATTTCTATGGCATTGAATATTGGGACATTCCAGCCGATTCTTTATGTCCTCCCATTCCTGGGCGTGCCGATTA
CGTGCACCATCTGGCCGATCTATTAGCCAGTTGCAATGGCGAGGTGATCCCTAAGGGCAAGAATATTGCGTTGCTGGATA
TTGGCGTCGGCGCTAACTGTATTTACCCAATTATTGGTCAGCGGGAGTACGGCTGGCGTTTTACCGGTACGGATATTGAC
TCACACGCGCTCAGTGCGGCAAAAATGGTGGTGTCGATGAACCCGACCCTGAAAAATACCTTGCGCCTGAAGCAGCAAAA
AGATCCACACGCTATTTTTGAAGGGGTGTGGGCGGTGAATGAGCGTTACGATGCGACGCTGTGTAACCCGCCATTCCATG
GTTCAGCAGAAGAAGCGGCGGCCACCACTCGCCGTAAATTGCACAAACTGGGGAAAAATGAAGTCGCTGCAAAGCCAGTA
CAGAACTTCGGTGGTAAAAACAGTGAGTTATGGTGTGAAGGTGGGGAGGAAGGGTTTGTCAGCCGCATGGTGGCAGAGAG
CGTAGCGAAAGCGCAGAACTGTTTCTGGTTTACCTCATTGATTTCCAAGAAGACCACGTTACCGGCGATTTATCATGCTC
TAAGATATGTGAAGGCAGTTGAGGTGCGCACTATTGAGATGGCGCAAGGGCAAAAAGTCAGTCGGTTTGTGGCCTGGACG
TTCCTGACGCCCGAGCAGCAAGCCGCGTGGGTTGCAGAGCGCTGGGCATAA

Upstream 100 bases:

>100_bases
CGATGGGCCAGCAGCGGATCATCATGTTGTTTGACATCGGCCGCACAAAGGGGCTGGTGGTGTCAAACCTATTCACCGTA
CACCCGTTTAGCTGTAATTT

Downstream 100 bases:

>100_bases
CGTTTCCACTATATAAGCCAAATAAGCCAGTGACCACGATAAGTCAGTGATCACGATCAGCCAGTTACCGTGGTTAACGA
ACCAGCATGGCGCAACGCTA

Product: putative SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA mA1618 methyltransferase; rRNA adenine N-6-methyltransferase [H]

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MLSYAPENAYQRASTMENKKVFPKEKSGLHPRNRHCSRYDFDALSVSCPELIPFLAPTAYGDISVDFADPLAVKMLNKAL
LKHFYGIEYWDIPADSLCPPIPGRADYVHHLADLLASCNGEVIPKGKNIALLDIGVGANCIYPIIGQREYGWRFTGTDID
SHALSAAKMVVSMNPTLKNTLRLKQQKDPHAIFEGVWAVNERYDATLCNPPFHGSAEEAAATTRRKLHKLGKNEVAAKPV
QNFGGKNSELWCEGGEEGFVSRMVAESVAKAQNCFWFTSLISKKTTLPAIYHALRYVKAVEVRTIEMAQGQKVSRFVAWT
FLTPEQQAAWVAERWA

Sequences:

>Translated_336_residues
MLSYAPENAYQRASTMENKKVFPKEKSGLHPRNRHCSRYDFDALSVSCPELIPFLAPTAYGDISVDFADPLAVKMLNKAL
LKHFYGIEYWDIPADSLCPPIPGRADYVHHLADLLASCNGEVIPKGKNIALLDIGVGANCIYPIIGQREYGWRFTGTDID
SHALSAAKMVVSMNPTLKNTLRLKQQKDPHAIFEGVWAVNERYDATLCNPPFHGSAEEAAATTRRKLHKLGKNEVAAKPV
QNFGGKNSELWCEGGEEGFVSRMVAESVAKAQNCFWFTSLISKKTTLPAIYHALRYVKAVEVRTIEMAQGQKVSRFVAWT
FLTPEQQAAWVAERWA
>Mature_336_residues
MLSYAPENAYQRASTMENKKVFPKEKSGLHPRNRHCSRYDFDALSVSCPELIPFLAPTAYGDISVDFADPLAVKMLNKAL
LKHFYGIEYWDIPADSLCPPIPGRADYVHHLADLLASCNGEVIPKGKNIALLDIGVGANCIYPIIGQREYGWRFTGTDID
SHALSAAKMVVSMNPTLKNTLRLKQQKDPHAIFEGVWAVNERYDATLCNPPFHGSAEEAAATTRRKLHKLGKNEVAAKPV
QNFGGKNSELWCEGGEEGFVSRMVAESVAKAQNCFWFTSLISKKTTLPAIYHALRYVKAVEVRTIEMAQGQKVSRFVAWT
FLTPEQQAAWVAERWA

Specific function: Specifically methylates the adenine in position 1618 of 23S rRNA [H]

COG id: COG3129

COG function: function code R; Predicted SAM-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. METT10D/rlmF family [H]

Homologues:

Organism=Homo sapiens, GI122114654, Length=295, Percent_Identity=29.8305084745763, Blast_Score=138, Evalue=6e-33,
Organism=Escherichia coli, GI87081786, Length=307, Percent_Identity=57.6547231270358, Blast_Score=358, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI32564931, Length=318, Percent_Identity=27.6729559748428, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI32564933, Length=318, Percent_Identity=25.1572327044025, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI281363415, Length=308, Percent_Identity=27.9220779220779, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI19922302, Length=308, Percent_Identity=27.9220779220779, Blast_Score=122, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016909
- InterPro:   IPR010286 [H]

Pfam domain/function: PF05971 Methyltransf_10 [H]

EC number: =2.1.1.181 [H]

Molecular weight: Translated: 37477; Mature: 37477

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSYAPENAYQRASTMENKKVFPKEKSGLHPRNRHCSRYDFDALSVSCPELIPFLAPTAY
CCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCHHHHHHHCCCCC
GDISVDFADPLAVKMLNKALLKHFYGIEYWDIPADSLCPPIPGRADYVHHLADLLASCNG
CCEEECCCCHHHHHHHHHHHHHHHCCCCEECCCHHHCCCCCCCHHHHHHHHHHHHHHCCC
EVIPKGKNIALLDIGVGANCIYPIIGQREYGWRFTGTDIDSHALSAAKMVVSMNPTLKNT
CEEECCCCEEEEEECCCCCEEECCCCCCCCCCEEECCCCCHHHHHHHHHHEECCCCHHHH
LRLKQQKDPHAIFEGVWAVNERYDATLCNPPFHGSAEEAAATTRRKLHKLGKNEVAAKPV
HHHHCCCCHHHHHHHHHHHCCCCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCHH
QNFGGKNSELWCEGGEEGFVSRMVAESVAKAQNCFWFTSLISKKTTLPAIYHALRYVKAV
HHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
EVRTIEMAQGQKVSRFVAWTFLTPEQQAAWVAERWA
HHEEEEHHCCCCHHHEEEEEEECCHHHHHHHHHHCC
>Mature Secondary Structure
MLSYAPENAYQRASTMENKKVFPKEKSGLHPRNRHCSRYDFDALSVSCPELIPFLAPTAY
CCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCHHHHHHHCCCCC
GDISVDFADPLAVKMLNKALLKHFYGIEYWDIPADSLCPPIPGRADYVHHLADLLASCNG
CCEEECCCCHHHHHHHHHHHHHHHCCCCEECCCHHHCCCCCCCHHHHHHHHHHHHHHCCC
EVIPKGKNIALLDIGVGANCIYPIIGQREYGWRFTGTDIDSHALSAAKMVVSMNPTLKNT
CEEECCCCEEEEEECCCCCEEECCCCCCCCCCEEECCCCCHHHHHHHHHHEECCCCHHHH
LRLKQQKDPHAIFEGVWAVNERYDATLCNPPFHGSAEEAAATTRRKLHKLGKNEVAAKPV
HHHHCCCCHHHHHHHHHHHCCCCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCHH
QNFGGKNSELWCEGGEEGFVSRMVAESVAKAQNCFWFTSLISKKTTLPAIYHALRYVKAV
HHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
EVRTIEMAQGQKVSRFVAWTFLTPEQQAAWVAERWA
HHEEEEHHCCCCHHHEEEEEEECCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA