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The map label for this gene is hmp

Identifier: 108811394

GI number: 108811394

Start: 1398750

End: 1399940

Strand: Reverse

Name: hmp

Synonym: YPN_1231

Alternate gene names: 108811394

Gene position: 1399940-1398750 (Counterclockwise)

Preceding gene: 108811398

Following gene: 108811393

Centisome position: 30.87

GC content: 46.85

Gene sequence:

>1191_bases
ATGCTGGATACCCAAACCATCGCTATCGTTAAATCTACCATCCCCTTGCTGGCTGCCACCGGCCCCAAACTGACAGCCCA
TTTCTATGAGCGCATGTTTAAGCATCACCCTGAACTCAAAAACATTTTTAATATGAGTAACCAATCGAGTGGTGATCAAC
GCGAAGCCTTATTTAATGCTATTTGTGCTTATGCGACCAATATCGAAAATCTTGCGGCACTACTCCCCACGGTTGAACGT
ATTGCCCAAAAACATACCAGTTTAAATATTCAGCCAGAGCATTACCCTATTGTTGGTGAGCATTTAATCGCTACCCTTGA
TGAACTATTTTCACCCGGTCAGGCCGTTTTAGATGCCTGGGCTAAAGCCTATGGTGTCTTGGCTGATGTGTTTATTCAGC
GTGAAAGCCAGATTTATCAGCAAAGTGAGACGGAAACCGGTGGTTGGAGAACCTTACGCCGCTTCCGCATTATAAAAAAA
GAGCAACAAAGCGAGGTAATTTGCAGTTTTGTCTTGGCACCGGAAGACGGCGGCCAAGTGCTTCACTATAAACCGGGGCA
ATATTTAGGCATTTATATCGAGCATGAAAGCCTTGAATTCCAAGAGATTCGTCAATATTCGCTGACCACCGCGCCCAATG
GCAAAACTTACCGTATTGCAGTAAAACGTGAAGAACAAGGCACGGTTTCAAACTTACTGCATCGGGAGTTAAATGAAGGG
GATATCGTGCGCATCGCACCACCCCGTGGCGATTTCTTTTTGGATGTATCACCAGATACCCCAGTAGCACTGATTTCTGC
TGGGGTGGGCCAAACCCCGATGCTCAGTATGCTCAATACCCTCTATAGCCAACAACATGCTGCCCCCGTACATTGGTTAC
ACGCAGCTGAAAATGGTCGTGTACACGCATTCGCTGATGAAGTTAGCGCTATCGCAGCCAAGATGCCTAATCTAAGCCGC
CATGTGTGGTACCGCGAGCCAGACCTACAGGATAAACACGGCGAGGATTACCATAGCCAAGGGTTGATGGATCTGAGTTC
GTACCAGTGGCTAGCTGATGATCCTAAACGACATTATTACTTCTGTGGCCCGCTGCCATTTATGCAATTTATAGGTCGTC
AATTACTGGCCCAAGGGATAGCACCTGAGCAGATTCACTACGAATGCTTCGGTCCACATAAAGTCATTTAA

Upstream 100 bases:

>100_bases
ACAGGTGAAAATTACATTTACAAAGAGAAGAGAAAACCATAAGATGCATTTAAAATACATGTATTAATTTATATATTATT
AAAACACTTAAGGAGCCACT

Downstream 100 bases:

>100_bases
CTGTTCATTTTTATCCTATCTCTACGCACTTAATGCCGCTGATCAGGAAGGTACCTGATCAGCATTTCGGCTATTTCAAT
TCTCCCATTTATTACATTTA

Product: nitric oxide dioxygenase

Products: NA

Alternate protein names: Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MLDTQTIAIVKSTIPLLAATGPKLTAHFYERMFKHHPELKNIFNMSNQSSGDQREALFNAICAYATNIENLAALLPTVER
IAQKHTSLNIQPEHYPIVGEHLIATLDELFSPGQAVLDAWAKAYGVLADVFIQRESQIYQQSETETGGWRTLRRFRIIKK
EQQSEVICSFVLAPEDGGQVLHYKPGQYLGIYIEHESLEFQEIRQYSLTTAPNGKTYRIAVKREEQGTVSNLLHRELNEG
DIVRIAPPRGDFFLDVSPDTPVALISAGVGQTPMLSMLNTLYSQQHAAPVHWLHAAENGRVHAFADEVSAIAAKMPNLSR
HVWYREPDLQDKHGEDYHSQGLMDLSSYQWLADDPKRHYYFCGPLPFMQFIGRQLLAQGIAPEQIHYECFGPHKVI

Sequences:

>Translated_396_residues
MLDTQTIAIVKSTIPLLAATGPKLTAHFYERMFKHHPELKNIFNMSNQSSGDQREALFNAICAYATNIENLAALLPTVER
IAQKHTSLNIQPEHYPIVGEHLIATLDELFSPGQAVLDAWAKAYGVLADVFIQRESQIYQQSETETGGWRTLRRFRIIKK
EQQSEVICSFVLAPEDGGQVLHYKPGQYLGIYIEHESLEFQEIRQYSLTTAPNGKTYRIAVKREEQGTVSNLLHRELNEG
DIVRIAPPRGDFFLDVSPDTPVALISAGVGQTPMLSMLNTLYSQQHAAPVHWLHAAENGRVHAFADEVSAIAAKMPNLSR
HVWYREPDLQDKHGEDYHSQGLMDLSSYQWLADDPKRHYYFCGPLPFMQFIGRQLLAQGIAPEQIHYECFGPHKVI
>Mature_396_residues
MLDTQTIAIVKSTIPLLAATGPKLTAHFYERMFKHHPELKNIFNMSNQSSGDQREALFNAICAYATNIENLAALLPTVER
IAQKHTSLNIQPEHYPIVGEHLIATLDELFSPGQAVLDAWAKAYGVLADVFIQRESQIYQQSETETGGWRTLRRFRIIKK
EQQSEVICSFVLAPEDGGQVLHYKPGQYLGIYIEHESLEFQEIRQYSLTTAPNGKTYRIAVKREEQGTVSNLLHRELNEG
DIVRIAPPRGDFFLDVSPDTPVALISAGVGQTPMLSMLNTLYSQQHAAPVHWLHAAENGRVHAFADEVSAIAAKMPNLSR
HVWYREPDLQDKHGEDYHSQGLMDLSSYQWLADDPKRHYYFCGPLPFMQFIGRQLLAQGIAPEQIHYECFGPHKVI

Specific function: Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a centra

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain

Homologues:

Organism=Escherichia coli, GI1788903, Length=396, Percent_Identity=63.1313131313131, Blast_Score=535, Evalue=1e-153,
Organism=Escherichia coli, GI1787098, Length=201, Percent_Identity=22.8855721393035, Blast_Score=64, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6321673, Length=413, Percent_Identity=31.9612590799031, Blast_Score=202, Evalue=7e-53,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): HMP_YERPE (Q8ZCR0)

Other databases:

- EMBL:   AL590842
- EMBL:   AE009952
- EMBL:   AE017042
- PIR:   AD0354
- RefSeq:   NP_668643.1
- RefSeq:   NP_993866.1
- RefSeq:   YP_002347842.1
- ProteinModelPortal:   Q8ZCR0
- SMR:   Q8ZCR0
- IntAct:   Q8ZCR0
- GeneID:   1146268
- GeneID:   1175732
- GeneID:   2766713
- GenomeReviews:   AE009952_GR
- GenomeReviews:   AE017042_GR
- GenomeReviews:   AL590842_GR
- KEGG:   ype:YPO2908
- KEGG:   ypk:y1321
- KEGG:   ypm:YP_2547
- HOGENOM:   HBG623097
- OMA:   QIGHKHR
- ProtClustDB:   PRK13289
- BioCyc:   YPES187410:Y1321-MONOMER
- BioCyc:   YPES214092:YPO2908-MONOMER
- BRENDA:   1.14.12.17
- HAMAP:   MF_01252
- InterPro:   IPR017927
- InterPro:   IPR001709
- InterPro:   IPR012292
- InterPro:   IPR009050
- InterPro:   IPR000971
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938
- Gene3D:   G3DSA:1.10.490.10
- PRINTS:   PR00371
- PRINTS:   PR00410

Pfam domain/function: PF00970 FAD_binding_6; PF00042 Globin; PF00175 NAD_binding_1; SSF46458 Globin_like; SSF63380 Riboflavin_synthase_like_b-brl

EC number: =1.14.12.17

Molecular weight: Translated: 44816; Mature: 44816

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS51384 FAD_FR; PS01033 GLOBIN

Important sites: ACT_SITE 95-95 ACT_SITE 135-135 BINDING 188-188

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDTQTIAIVKSTIPLLAATGPKLTAHFYERMFKHHPELKNIFNMSNQSSGDQREALFNA
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHH
ICAYATNIENLAALLPTVERIAQKHTSLNIQPEHYPIVGEHLIATLDELFSPGQAVLDAW
HHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHH
AKAYGVLADVFIQRESQIYQQSETETGGWRTLRRFRIIKKEQQSEVICSFVLAPEDGGQV
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCEE
LHYKPGQYLGIYIEHESLEFQEIRQYSLTTAPNGKTYRIAVKREEQGTVSNLLHRELNEG
EEECCCCEEEEEEEECCCCHHHHHHHHCEECCCCCEEEEEEEECCCCHHHHHHHHHCCCC
DIVRIAPPRGDFFLDVSPDTPVALISAGVGQTPMLSMLNTLYSQQHAAPVHWLHAAENGR
CEEEEECCCCCEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCC
VHAFADEVSAIAAKMPNLSRHVWYREPDLQDKHGEDYHSQGLMDLSSYQWLADDPKRHYY
EEEHHHHHHHHHHHCCCCHHHEEECCCCCCCCCCCCHHHCCCCCHHCCCCCCCCCCCCEE
FCGPLPFMQFIGRQLLAQGIAPEQIHYECFGPHKVI
EECCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCC
>Mature Secondary Structure
MLDTQTIAIVKSTIPLLAATGPKLTAHFYERMFKHHPELKNIFNMSNQSSGDQREALFNA
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHH
ICAYATNIENLAALLPTVERIAQKHTSLNIQPEHYPIVGEHLIATLDELFSPGQAVLDAW
HHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHH
AKAYGVLADVFIQRESQIYQQSETETGGWRTLRRFRIIKKEQQSEVICSFVLAPEDGGQV
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCEE
LHYKPGQYLGIYIEHESLEFQEIRQYSLTTAPNGKTYRIAVKREEQGTVSNLLHRELNEG
EEECCCCEEEEEEEECCCCHHHHHHHHCEECCCCCEEEEEEEECCCCHHHHHHHHHCCCC
DIVRIAPPRGDFFLDVSPDTPVALISAGVGQTPMLSMLNTLYSQQHAAPVHWLHAAENGR
CEEEEECCCCCEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCC
VHAFADEVSAIAAKMPNLSRHVWYREPDLQDKHGEDYHSQGLMDLSSYQWLADDPKRHYY
EEEHHHHHHHHHHHCCCCHHHEEECCCCCCCCCCCCHHHCCCCCHHCCCCCCCCCCCCEE
FCGPLPFMQFIGRQLLAQGIAPEQIHYECFGPHKVI
EECCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430