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The map label for this gene is proW [H]

Identifier: 108811295

GI number: 108811295

Start: 1299052

End: 1300218

Strand: Reverse

Name: proW [H]

Synonym: YPN_1132

Alternate gene names: 108811295

Gene position: 1300218-1299052 (Counterclockwise)

Preceding gene: 108811296

Following gene: 108811294

Centisome position: 28.67

GC content: 54.41

Gene sequence:

>1167_bases
ATGAGTGATCAAATTCAGGTGAACGAACAAACAGTCAATAATCCGTGGGCAACTGATACGGCAACATCAGTCGCTCCCAC
GGTTACCGATAGTGCACCTCAGAGCCTGTCAGCCGCTGCTGACCCATGGGGAGCAAGCATGGCCGAGGGAAGCAATAGCG
TAGGGTCTGCAACGCATGAGGCGGCGTCTCAGGCCGCACAAAATACCGATTGGTTGAACAGCGCACCTACCCCAGCACCA
GAGCATTTCAGCCTGATGGACCCGTTCCAGAATACGTGGATCCCACTGGACTCGTGGGTTACCCAAGGTATTGATTGGGT
CGTTCTGCACTTTCGTCCGCTGTTTCAGGGGATCCGCGTACCGGTTGATTTTATCCTCAGCGGCTTTCAGACATTGTTAC
TGGGGATGCCCGCACCCGTAGCGATTTTAGTTTTCGCCCTGATAGCCTGGCAGTTTTCCACGCTCAGTATGGGCGCGGCA
ACCTTGATATCACTGATTGCCATCGGTGCTATCGGCGCATGGTCGCAGGCCATGGTGACCTTGGCACTGGTTCTGACCTC
CCTGTTCTTCTGTATTCTGATTGGGCTCCCCCTCGGAATCTGGCTGGCGCGCAGCAATAATGCCGCCCGGATTATTCGGC
CCTTACTGGATGCAATGCAGACCACACCCGCGTTCGTCTATCTGGTCCCCATCGTTATGCTGTTCGGTATCGGTAACGTC
CCCGGTGTCGTGGTGACGATTATTTTTGCCCTGCCCCCTATTGTCCGCCTGACCATTCTCGGCATCAAACAAGTACCGGA
AGATCTGATTGAAGCAGCGGAGTCCTTTGGTGCCAACCCGCGTCAGTTGCTGTTCAAAGTACAGCTACCGCTGGCGATGC
CAACCATTATGGCTGGGGTGAACCAAACGTTGATGCTGGCATTGTCCATGGTGGTTATCGCTTCAATGATTGCAGTGGGT
GGTTTAGGTCAGATGGTCCTGCGCGGTATTGGCCGTCTGGATATGGGGCTGGCTGCCGTAGGCGGCGTCGGTATTGTGAT
CCTCGCCATTATTTTGGATCGCTTAACCCAATCATTAGGTCGTGATCGCCGCAGTAAAGGCACTGGTCGCTGGTATACCA
CAGGGCCTGTTGGGTTGCTCACCAAACCCTTCTGTAACAGCAAATAA

Upstream 100 bases:

>100_bases
CAGGCTCCGTGTGCGGTCCCGGTGATCGGTGAAGACAGTAGCTATATCGGTATTATTTCAAAAGCCATGCTCTTGCAGGC
CCTAGATAAGGAGACGCCAA

Downstream 100 bases:

>100_bases
TTTCAATTAATTACGCTCAAATAAAATAGCTTAAAAAAGGAAAAACTATGCGCACGACAGGAATCTGGGCCTTGGCCCTC
ACCACACTGATCAGCTCTCA

Product: glycine betaine transporter membrane protein

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 388; Mature: 387

Protein sequence:

>388_residues
MSDQIQVNEQTVNNPWATDTATSVAPTVTDSAPQSLSAAADPWGASMAEGSNSVGSATHEAASQAAQNTDWLNSAPTPAP
EHFSLMDPFQNTWIPLDSWVTQGIDWVVLHFRPLFQGIRVPVDFILSGFQTLLLGMPAPVAILVFALIAWQFSTLSMGAA
TLISLIAIGAIGAWSQAMVTLALVLTSLFFCILIGLPLGIWLARSNNAARIIRPLLDAMQTTPAFVYLVPIVMLFGIGNV
PGVVVTIIFALPPIVRLTILGIKQVPEDLIEAAESFGANPRQLLFKVQLPLAMPTIMAGVNQTLMLALSMVVIASMIAVG
GLGQMVLRGIGRLDMGLAAVGGVGIVILAIILDRLTQSLGRDRRSKGTGRWYTTGPVGLLTKPFCNSK

Sequences:

>Translated_388_residues
MSDQIQVNEQTVNNPWATDTATSVAPTVTDSAPQSLSAAADPWGASMAEGSNSVGSATHEAASQAAQNTDWLNSAPTPAP
EHFSLMDPFQNTWIPLDSWVTQGIDWVVLHFRPLFQGIRVPVDFILSGFQTLLLGMPAPVAILVFALIAWQFSTLSMGAA
TLISLIAIGAIGAWSQAMVTLALVLTSLFFCILIGLPLGIWLARSNNAARIIRPLLDAMQTTPAFVYLVPIVMLFGIGNV
PGVVVTIIFALPPIVRLTILGIKQVPEDLIEAAESFGANPRQLLFKVQLPLAMPTIMAGVNQTLMLALSMVVIASMIAVG
GLGQMVLRGIGRLDMGLAAVGGVGIVILAIILDRLTQSLGRDRRSKGTGRWYTTGPVGLLTKPFCNSK
>Mature_387_residues
SDQIQVNEQTVNNPWATDTATSVAPTVTDSAPQSLSAAADPWGASMAEGSNSVGSATHEAASQAAQNTDWLNSAPTPAPE
HFSLMDPFQNTWIPLDSWVTQGIDWVVLHFRPLFQGIRVPVDFILSGFQTLLLGMPAPVAILVFALIAWQFSTLSMGAAT
LISLIAIGAIGAWSQAMVTLALVLTSLFFCILIGLPLGIWLARSNNAARIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVP
GVVVTIIFALPPIVRLTILGIKQVPEDLIEAAESFGANPRQLLFKVQLPLAMPTIMAGVNQTLMLALSMVVIASMIAVGG
LGQMVLRGIGRLDMGLAAVGGVGIVILAIILDRLTQSLGRDRRSKGTGRWYTTGPVGLLTKPFCNSK

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine/L-proline [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=330, Percent_Identity=82.1212121212121, Blast_Score=517, Evalue=1e-148,
Organism=Escherichia coli, GI1788451, Length=195, Percent_Identity=31.7948717948718, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1786564, Length=154, Percent_Identity=32.4675324675325, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1788449, Length=181, Percent_Identity=29.8342541436464, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 41204; Mature: 41073

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDQIQVNEQTVNNPWATDTATSVAPTVTDSAPQSLSAAADPWGASMAEGSNSVGSATHE
CCCCEECCHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCHHHCCCCCCCHHHHH
AASQAAQNTDWLNSAPTPAPEHFSLMDPFQNTWIPLDSWVTQGIDWVVLHFRPLFQGIRV
HHHHHHCCCCCCCCCCCCCCCCCCHHCCHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHC
PVDFILSGFQTLLLGMPAPVAILVFALIAWQFSTLSMGAATLISLIAIGAIGAWSQAMVT
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
LALVLTSLFFCILIGLPLGIWLARSNNAARIIRPLLDAMQTTPAFVYLVPIVMLFGIGNV
HHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCC
PGVVVTIIFALPPIVRLTILGIKQVPEDLIEAAESFGANPRQLLFKVQLPLAMPTIMAGV
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHCC
NQTLMLALSMVVIASMIAVGGLGQMVLRGIGRLDMGLAAVGGVGIVILAIILDRLTQSLG
CHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RDRRSKGTGRWYTTGPVGLLTKPFCNSK
CHHHCCCCCCEEECCCHHHHCCCCCCCC
>Mature Secondary Structure 
SDQIQVNEQTVNNPWATDTATSVAPTVTDSAPQSLSAAADPWGASMAEGSNSVGSATHE
CCCEECCHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCHHHCCCCCCCHHHHH
AASQAAQNTDWLNSAPTPAPEHFSLMDPFQNTWIPLDSWVTQGIDWVVLHFRPLFQGIRV
HHHHHHCCCCCCCCCCCCCCCCCCHHCCHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHC
PVDFILSGFQTLLLGMPAPVAILVFALIAWQFSTLSMGAATLISLIAIGAIGAWSQAMVT
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
LALVLTSLFFCILIGLPLGIWLARSNNAARIIRPLLDAMQTTPAFVYLVPIVMLFGIGNV
HHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCC
PGVVVTIIFALPPIVRLTILGIKQVPEDLIEAAESFGANPRQLLFKVQLPLAMPTIMAGV
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHCC
NQTLMLALSMVVIASMIAVGGLGQMVLRGIGRLDMGLAAVGGVGIVILAIILDRLTQSLG
CHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RDRRSKGTGRWYTTGPVGLLTKPFCNSK
CHHHCCCCCCEEECCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2649479; 9205837; 9278503 [H]