Click here to switch to the map view.

The map label for this gene is mrdB [H]

Identifier: 108811252

GI number: 108811252

Start: 1250346

End: 1251458

Strand: Reverse

Name: mrdB [H]

Synonym: YPN_1088

Alternate gene names: 108811252

Gene position: 1251458-1250346 (Counterclockwise)

Preceding gene: 108811253

Following gene: 108811251

Centisome position: 27.6

GC content: 47.8

Gene sequence:

>1113_bases
ATGACTGATAATCAACAAAAAGGCTCGTTGTGGTACAAACTACACATCGACCTACCGTTTCTGATTTGCATCTTGGCGCT
GCTGGCTTATAGCGCCTTTGTGATGTGGAGTGCCAGTGGGCAAGATATTGGCATGATGGAACGCAAAGTTGGCCAAATTG
CTATGGGCTTGATTGTCATGTTGGTCATGGCACAGATCCCGCCACGGGTGTATGAAAGCTGGGCCCCCTATCTCTATTTT
GTTTGTGTCATATTACTGATACTGGTCGATGCCTTTGGGCAGATAAGCAAAGGCGCTCAACGCTGGTTGGACTTAGGTTT
TATCCGCTTTCAGCCTTCTGAAATCGCTAAGATTGCCGTCCCCCTGATGGTTGCACGCTTTATGAACCGCGATGTTTGTC
CACCCTCACTGAAAAATACCGGCATCGCACTGATTTTAATTTTCATCCCCACCTTGTTGGTTGCTGCTCAACCCGATCTG
GGAACATCAATTCTGGTTGCGGCCTCCGGCCTGTTTGTGTTGTTCCTTTCGGGTATGAGCTGGCGCTTAATTGGGGTTGC
GGTTGTACTGTTGGCCGGGTTTATCCCCATTCTCTGGTTCTTCCTGATGCATGGTTATCAACGTGATCGCGTCATGATGC
TGTTGGATCCAGAAAGCGATCCGCTCGGTGCGGGTTACCATATTATTCAGTCTAAAATTGCGATTGGCTCTGGTGGTTTA
TCTGGAAAAGGCTGGTTGCACGGCACTCAGTCACAGCTAGAGTTCTTACCTGAACGCCATACTGACTTCATTTTTGCCGT
ATTGGCTGAAGAGCTCGGTTTAATCGGTGTGCTGGTCTTATTAGGCCTCTATTTATGTTTGATCATGCGCGGCTTGGTCA
TTGCTGCAAATGCACAAACCACCTTCGGCCGGGTCATGGTTGGCGGGTTGATGCTGATACTCTTTGTGTATGTATTTGTT
AACATAGGTATGGTCAGTGGCATTTTACCGGTGGTTGGCGTACCTTTGCCCTTAGTCAGTTATGGAGGCTCGGCGCTAGT
AGTTCTGATGGCCGGGTTTGGTATCGTGATGTCGATACATACTCATCGAAAAATGTTATCTAAGAATTTATAG

Upstream 100 bases:

>100_bases
CATTGAAGCCGATCACGTTAAGATAGGTTACGTTAAGACAAGTCACGTTAAGACAGGTCACGTTGAAAATAAATAGCGTT
GGAGCGGGTTAAGGTAACTC

Downstream 100 bases:

>100_bases
AGGTGGGCAATGCGTAAGGAATGGCTTTGGGTCGGCATCGCAAGTGTGCTGCTATCAGCATGTATTGATCAGCCTCCGGC
CCCCCAGCAACAAGTACAAC

Product: cell wall shape-determining protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MTDNQQKGSLWYKLHIDLPFLICILALLAYSAFVMWSASGQDIGMMERKVGQIAMGLIVMLVMAQIPPRVYESWAPYLYF
VCVILLILVDAFGQISKGAQRWLDLGFIRFQPSEIAKIAVPLMVARFMNRDVCPPSLKNTGIALILIFIPTLLVAAQPDL
GTSILVAASGLFVLFLSGMSWRLIGVAVVLLAGFIPILWFFLMHGYQRDRVMMLLDPESDPLGAGYHIIQSKIAIGSGGL
SGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLIGVLVLLGLYLCLIMRGLVIAANAQTTFGRVMVGGLMLILFVYVFV
NIGMVSGILPVVGVPLPLVSYGGSALVVLMAGFGIVMSIHTHRKMLSKNL

Sequences:

>Translated_370_residues
MTDNQQKGSLWYKLHIDLPFLICILALLAYSAFVMWSASGQDIGMMERKVGQIAMGLIVMLVMAQIPPRVYESWAPYLYF
VCVILLILVDAFGQISKGAQRWLDLGFIRFQPSEIAKIAVPLMVARFMNRDVCPPSLKNTGIALILIFIPTLLVAAQPDL
GTSILVAASGLFVLFLSGMSWRLIGVAVVLLAGFIPILWFFLMHGYQRDRVMMLLDPESDPLGAGYHIIQSKIAIGSGGL
SGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLIGVLVLLGLYLCLIMRGLVIAANAQTTFGRVMVGGLMLILFVYVFV
NIGMVSGILPVVGVPLPLVSYGGSALVVLMAGFGIVMSIHTHRKMLSKNL
>Mature_369_residues
TDNQQKGSLWYKLHIDLPFLICILALLAYSAFVMWSASGQDIGMMERKVGQIAMGLIVMLVMAQIPPRVYESWAPYLYFV
CVILLILVDAFGQISKGAQRWLDLGFIRFQPSEIAKIAVPLMVARFMNRDVCPPSLKNTGIALILIFIPTLLVAAQPDLG
TSILVAASGLFVLFLSGMSWRLIGVAVVLLAGFIPILWFFLMHGYQRDRVMMLLDPESDPLGAGYHIIQSKIAIGSGGLS
GKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLIGVLVLLGLYLCLIMRGLVIAANAQTTFGRVMVGGLMLILFVYVFVN
IGMVSGILPVVGVPLPLVSYGGSALVVLMAGFGIVMSIHTHRKMLSKNL

Specific function: This is one of the proteins responsible for the rod shape of E.coli. Is required for the expression of the enzymatic activity of PBP2, which is thought to synthesize peptidoglycan in the step of initiation of cell elongation [H]

COG id: COG0772

COG function: function code D; Bacterial cell division membrane protein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ftsW/rodA/spoVE family [H]

Homologues:

Organism=Escherichia coli, GI1786853, Length=370, Percent_Identity=85.945945945946, Blast_Score=608, Evalue=1e-175,
Organism=Escherichia coli, GI1786277, Length=317, Percent_Identity=31.5457413249211, Blast_Score=159, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001182
- InterPro:   IPR018365
- InterPro:   IPR011923 [H]

Pfam domain/function: PF01098 FTSW_RODA_SPOVE [H]

EC number: NA

Molecular weight: Translated: 40442; Mature: 40310

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: PS00428 FTSW_RODA_SPOVE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDNQQKGSLWYKLHIDLPFLICILALLAYSAFVMWSASGQDIGMMERKVGQIAMGLIVM
CCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
LVMAQIPPRVYESWAPYLYFVCVILLILVDAFGQISKGAQRWLDLGFIRFQPSEIAKIAV
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECHHHHHHHHH
PLMVARFMNRDVCPPSLKNTGIALILIFIPTLLVAAQPDLGTSILVAASGLFVLFLSGMS
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
WRLIGVAVVLLAGFIPILWFFLMHGYQRDRVMMLLDPESDPLGAGYHIIQSKIAIGSGGL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCC
SGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLIGVLVLLGLYLCLIMRGLVIAANAQT
CCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCH
TFGRVMVGGLMLILFVYVFVNIGMVSGILPVVGVPLPLVSYGGSALVVLMAGFGIVMSIH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHH
THRKMLSKNL
HHHHHHHCCC
>Mature Secondary Structure 
TDNQQKGSLWYKLHIDLPFLICILALLAYSAFVMWSASGQDIGMMERKVGQIAMGLIVM
CCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
LVMAQIPPRVYESWAPYLYFVCVILLILVDAFGQISKGAQRWLDLGFIRFQPSEIAKIAV
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECHHHHHHHHH
PLMVARFMNRDVCPPSLKNTGIALILIFIPTLLVAAQPDLGTSILVAASGLFVLFLSGMS
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
WRLIGVAVVLLAGFIPILWFFLMHGYQRDRVMMLLDPESDPLGAGYHIIQSKIAIGSGGL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCC
SGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLIGVLVLLGLYLCLIMRGLVIAANAQT
CCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCH
TFGRVMVGGLMLILFVYVFVNIGMVSGILPVVGVPLPLVSYGGSALVVLMAGFGIVMSIH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHH
THRKMLSKNL
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]