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The map label for this gene is rsmE [H]

Identifier: 108800424

GI number: 108800424

Start: 3668101

End: 3668847

Strand: Reverse

Name: rsmE [H]

Synonym: Mmcs_3458

Alternate gene names: 108800424

Gene position: 3668847-3668101 (Counterclockwise)

Preceding gene: 108800425

Following gene: 108800422

Centisome position: 64.3

GC content: 75.37

Gene sequence:

>747_bases
GTGCGCGACCTCTTCTACGTCGACGCGCTGCCCGGCCTCGGTGAGCTCGCCGTCGTCGACGGCGACGCCGGTTTTCACGC
CACCAACGTGCGGCGCACCCGTGCCGGTGAACACCTCGACCTGGGTGACGGCGACGGCACCGTGGCGCACTGCGTGGTCG
AGGACGTCGGCAAGGGCCGGCTGACCGCACGCGTGCTGGAACGCCGCACCGTCACACCACCCGCGCCGGCAGTGACCGTC
GTCCAGGCGATCCCCAAATCCGACCGCGCGGAGCTGGCGGTGGAGTCGGCCACCGAGGCCGGCGCCGACGCGGTTCTCGC
GTGGCAGGCCGCCCGCTGCGTGGCGCGCTGGGACGGAGCCGGCAAGGTGGACAAGGGTCTGCGGCGCTGGCGTGCGGTGG
TCCGCTCCGCGGCGCGGCAGTCGCGGCGCGCCCACATCCCGCCGGTCGACGGTGTGGTGACGACGGCCGAGCTGGTGCGC
CGGGTGACCGCCGAGGTGGCCGACGGCGCGACGGTGCTGGTCCTGCACGAATCCGCCACCGGCCCGCTGACGGGCGTGGA
TCTGACGCAGGCCCGGGCGGTGACCCTGATCGTGGGACCCGAGGGCGGGATCGCCGACGAGGAGCTCGATGCCCTGACGG
CGGCGGGGGCGGTGGCGGTCCGCCTCGGTCCGACGGTGTTGCGCACCTCGACCGCGGCGGCGGTCGGGCTCGGCGCACTA
GGGGTGCTGACGCCGCGCTGGCGGTAG

Upstream 100 bases:

>100_bases
GCCGAGGTCCGCACCAGCAGTTCACCGGCCGCGTCGAACGCCGGCGGACTGTTCAGCCGGCTGCGCGAGACGTTCACCGG
CCGCTAGGAAGGCTCGTCGC

Downstream 100 bases:

>100_bases
TGCCCCCACCAACGAAGCGGCGGCGCACACCTGCCACGTCAGGATCGGGTAGACGCTCGCGCGCTCCCACACCGGATCGG
GCAGCATGACGATCCCGGTC

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MRDLFYVDALPGLGELAVVDGDAGFHATNVRRTRAGEHLDLGDGDGTVAHCVVEDVGKGRLTARVLERRTVTPPAPAVTV
VQAIPKSDRAELAVESATEAGADAVLAWQAARCVARWDGAGKVDKGLRRWRAVVRSAARQSRRAHIPPVDGVVTTAELVR
RVTAEVADGATVLVLHESATGPLTGVDLTQARAVTLIVGPEGGIADEELDALTAAGAVAVRLGPTVLRTSTAAAVGLGAL
GVLTPRWR

Sequences:

>Translated_248_residues
MRDLFYVDALPGLGELAVVDGDAGFHATNVRRTRAGEHLDLGDGDGTVAHCVVEDVGKGRLTARVLERRTVTPPAPAVTV
VQAIPKSDRAELAVESATEAGADAVLAWQAARCVARWDGAGKVDKGLRRWRAVVRSAARQSRRAHIPPVDGVVTTAELVR
RVTAEVADGATVLVLHESATGPLTGVDLTQARAVTLIVGPEGGIADEELDALTAAGAVAVRLGPTVLRTSTAAAVGLGAL
GVLTPRWR
>Mature_248_residues
MRDLFYVDALPGLGELAVVDGDAGFHATNVRRTRAGEHLDLGDGDGTVAHCVVEDVGKGRLTARVLERRTVTPPAPAVTV
VQAIPKSDRAELAVESATEAGADAVLAWQAARCVARWDGAGKVDKGLRRWRAVVRSAARQSRRAHIPPVDGVVTTAELVR
RVTAEVADGATVLVLHESATGPLTGVDLTQARAVTLIVGPEGGIADEELDALTAAGAVAVRLGPTVLRTSTAAAVGLGAL
GVLTPRWR

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=240, Percent_Identity=25.4166666666667, Blast_Score=78, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 25744; Mature: 25744

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDLFYVDALPGLGELAVVDGDAGFHATNVRRTRAGEHLDLGDGDGTVAHCVVEDVGKGR
CCCEEEEECCCCCCCEEEEECCCCCEECCCHHHCCCCCCCCCCCCCCEEHHHHHHCCCCH
LTARVLERRTVTPPAPAVTVVQAIPKSDRAELAVESATEAGADAVLAWQAARCVARWDGA
HHHHHHHHCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
GKVDKGLRRWRAVVRSAARQSRRAHIPPVDGVVTTAELVRRVTAEVADGATVLVLHESAT
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCC
GPLTGVDLTQARAVTLIVGPEGGIADEELDALTAAGAVAVRLGPTVLRTSTAAAVGLGAL
CCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHH
GVLTPRWR
HCCCCCCC
>Mature Secondary Structure
MRDLFYVDALPGLGELAVVDGDAGFHATNVRRTRAGEHLDLGDGDGTVAHCVVEDVGKGR
CCCEEEEECCCCCCCEEEEECCCCCEECCCHHHCCCCCCCCCCCCCCEEHHHHHHCCCCH
LTARVLERRTVTPPAPAVTVVQAIPKSDRAELAVESATEAGADAVLAWQAARCVARWDGA
HHHHHHHHCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
GKVDKGLRRWRAVVRSAARQSRRAHIPPVDGVVTTAELVRRVTAEVADGATVLVLHESAT
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCC
GPLTGVDLTQARAVTLIVGPEGGIADEELDALTAAGAVAVRLGPTVLRTSTAAAVGLGAL
CCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHH
GVLTPRWR
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]