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The map label for this gene is stp [H]

Identifier: 108797980

GI number: 108797980

Start: 1111722

End: 1113332

Strand: Reverse

Name: stp [H]

Synonym: Mmcs_1007

Alternate gene names: 108797980

Gene position: 1113332-1111722 (Counterclockwise)

Preceding gene: 108797982

Following gene: 108797979

Centisome position: 19.51

GC content: 68.4

Gene sequence:

>1611_bases
ATGGTCGACACCCTCCCGCGCACCGACCAGGATATCGACGCCGAGATCGCCGCTCTGTCGAAGCGGAAACGGATCTGGCT
GCTGGTCATCGCCAGTGTCGACGTGCTGATGGTCATCTCGTCGATGGTGGCGCTCAACGCGGCGCTGCCCGACATCGCGC
TGCAGACCTCCGCGACACAGTCCCAGTTGACCTGGATCGTCGACGGTTACACGCTGGCGCTGGCCTGCCTGCTGCTGCCG
GCGGGCGCCATCGGCGACCGCTACGGTCGGCGGGGTGCGCTCCTGGTGGGCCTCGCGATCTTCGCGGTGGCCTCGCTGGC
CCCGGTGCTGTTCGACAGCCCGATGCAGATCATCATCGCGCGGGCCGTCGCCGGCGTCGGCGCGGCGCTCATCATGCCCG
CCACCCTCTCGCTGCTCACCGCCGCGTTCCCGAAGTCCGAGCGCAACAAGGCCGTCGGCATCTGGGCCGGCGTGGCGGGG
TCGGGCGCGATCTTCGGCTTCCTCGGTACCGGGCTCCTGCTGAACTACTTCTCGTGGCAGTCGATCTTCTACATGTTCGC
CGGCGGGGCACTGCTGATGTTCGTGGCGACCTGCACCATCGGCTCTTCCCGCGACGAGACCGCCACCCCCATCGACTGGG
TGGGCGCCGCGCTGATCGGCACCGCGATCGCGGTGTTCGTGCTGGGGGTGGTCGAGGCGCCGGTACGCGGGTGGACCGAC
CCGGCAGTGCTCGGTTGTCTGGGCGCCGGGGTGGTGCTGGCCGGGTTGTTCGCCGTGGTCCAGCTGCGCCGTGCGCATCC
ACTGCTCGATGTCCGGTTGTTCCGACGGCCGGATTTCGCCACTGGCGCCGCAGGCATCACATTCCTGTTCATCGCGAACT
TCGGGTTCTTCTACGTCGCGATGCAGTTCATGCAGCTGGTCATGGGCTACAGCGCGCTGGAGACCGCATTCGCCTTGTCG
CCGTTGGCGTTCCCGGTGCTGATACTCGGCGGCACACTGCCTCTGTATCTGCCGAAGGTGGGTCTGCGCTTCGCGGTCAC
CGTTGGCCTTCTCCTGCTTGCCACGGGCCTGTTCCTCATGCGTTTCCTGGAGGCCGACGCGACCTTCCTCGACCTCATGT
GGCCAATGCTGCTCGCCGCATCGGGCATCGGACTGTGCACGGCGCCGACGACTTCGGCGATCATGAACGCCGTGCCTGAC
GAGAAGCAGGGCGTCGCCTCGGCGGTCAACGACGCCACCCGCGAGGTCGGTGCCGCCGTCGGCATCGCAGTGGCGGGATC
GGTCCTGGCCGCCGTGTACCAGAGCGCGCTGGCCCCGAAGCTCGGCGCTCTGCCCGAGCAGATCCGCGACGCCGCAACCG
ATTCGCTGGCCCACGCGCTGGCGATCTCCGAACAGATGGGTCCGCAGGGCGAACAGTTGGCCGACTTCGCTCGAGACGCG
TTCATGCAGGCCGCCGACCAGGCGTTGTTCGCACTCTCGGCGCTTCTGGTGGTCGGGGCGGTCTTCGTGGCGATCTGGTC
TCCCGGACGAGACGGACGACAGTGGGCCGCGATCCGGCGGCGGCGAGGAGCAGACGAGAACCGGTCGGCACCCGCGGAGG
TTGCGCCGTAG

Upstream 100 bases:

>100_bases
TCCACCTCCCCGGTGGGAACAAGACTATCGGTCCTGCTACGAGACCGTTAGTCTCGTTTCACGTCGAGGCATCGGTCTCG
CCCCTCCGAAGAGAGACGAC

Downstream 100 bases:

>100_bases
GCTTTCCAGGCGTGCGACTGCTCCTGGTGAACCCGAACACGACGGCGTCGATGACCGCCGCGATCGCCGCGTCCGCGTCC
GCAGTGGCGGACCCCGGTAC

Product: major facilitator transporter

Products: NA

Alternate protein names: Spectinomycin tetracycline efflux pump [H]

Number of amino acids: Translated: 536; Mature: 536

Protein sequence:

>536_residues
MVDTLPRTDQDIDAEIAALSKRKRIWLLVIASVDVLMVISSMVALNAALPDIALQTSATQSQLTWIVDGYTLALACLLLP
AGAIGDRYGRRGALLVGLAIFAVASLAPVLFDSPMQIIIARAVAGVGAALIMPATLSLLTAAFPKSERNKAVGIWAGVAG
SGAIFGFLGTGLLLNYFSWQSIFYMFAGGALLMFVATCTIGSSRDETATPIDWVGAALIGTAIAVFVLGVVEAPVRGWTD
PAVLGCLGAGVVLAGLFAVVQLRRAHPLLDVRLFRRPDFATGAAGITFLFIANFGFFYVAMQFMQLVMGYSALETAFALS
PLAFPVLILGGTLPLYLPKVGLRFAVTVGLLLLATGLFLMRFLEADATFLDLMWPMLLAASGIGLCTAPTTSAIMNAVPD
EKQGVASAVNDATREVGAAVGIAVAGSVLAAVYQSALAPKLGALPEQIRDAATDSLAHALAISEQMGPQGEQLADFARDA
FMQAADQALFALSALLVVGAVFVAIWSPGRDGRQWAAIRRRRGADENRSAPAEVAP

Sequences:

>Translated_536_residues
MVDTLPRTDQDIDAEIAALSKRKRIWLLVIASVDVLMVISSMVALNAALPDIALQTSATQSQLTWIVDGYTLALACLLLP
AGAIGDRYGRRGALLVGLAIFAVASLAPVLFDSPMQIIIARAVAGVGAALIMPATLSLLTAAFPKSERNKAVGIWAGVAG
SGAIFGFLGTGLLLNYFSWQSIFYMFAGGALLMFVATCTIGSSRDETATPIDWVGAALIGTAIAVFVLGVVEAPVRGWTD
PAVLGCLGAGVVLAGLFAVVQLRRAHPLLDVRLFRRPDFATGAAGITFLFIANFGFFYVAMQFMQLVMGYSALETAFALS
PLAFPVLILGGTLPLYLPKVGLRFAVTVGLLLLATGLFLMRFLEADATFLDLMWPMLLAASGIGLCTAPTTSAIMNAVPD
EKQGVASAVNDATREVGAAVGIAVAGSVLAAVYQSALAPKLGALPEQIRDAATDSLAHALAISEQMGPQGEQLADFARDA
FMQAADQALFALSALLVVGAVFVAIWSPGRDGRQWAAIRRRRGADENRSAPAEVAP
>Mature_536_residues
MVDTLPRTDQDIDAEIAALSKRKRIWLLVIASVDVLMVISSMVALNAALPDIALQTSATQSQLTWIVDGYTLALACLLLP
AGAIGDRYGRRGALLVGLAIFAVASLAPVLFDSPMQIIIARAVAGVGAALIMPATLSLLTAAFPKSERNKAVGIWAGVAG
SGAIFGFLGTGLLLNYFSWQSIFYMFAGGALLMFVATCTIGSSRDETATPIDWVGAALIGTAIAVFVLGVVEAPVRGWTD
PAVLGCLGAGVVLAGLFAVVQLRRAHPLLDVRLFRRPDFATGAAGITFLFIANFGFFYVAMQFMQLVMGYSALETAFALS
PLAFPVLILGGTLPLYLPKVGLRFAVTVGLLLLATGLFLMRFLEADATFLDLMWPMLLAASGIGLCTAPTTSAIMNAVPD
EKQGVASAVNDATREVGAAVGIAVAGSVLAAVYQSALAPKLGALPEQIRDAATDSLAHALAISEQMGPQGEQLADFARDA
FMQAADQALFALSALLVVGAVFVAIWSPGRDGRQWAAIRRRRGADENRSAPAEVAP

Specific function: Contributes to spectinomycin and tetracycline resistance [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI87081983, Length=385, Percent_Identity=25.4545454545455, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1789042, Length=397, Percent_Identity=24.1813602015113, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1788710, Length=480, Percent_Identity=22.7083333333333, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1790195, Length=403, Percent_Identity=24.8138957816377, Blast_Score=83, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6324954, Length=409, Percent_Identity=24.4498777506112, Blast_Score=84, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 56044; Mature: 56044

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDTLPRTDQDIDAEIAALSKRKRIWLLVIASVDVLMVISSMVALNAALPDIALQTSATQ
CCCCCCCCCCCHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCH
SQLTWIVDGYTLALACLLLPAGAIGDRYGRRGALLVGLAIFAVASLAPVLFDSPMQIIIA
HHHHEEECHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
RAVAGVGAALIMPATLSLLTAAFPKSERNKAVGIWAGVAGSGAIFGFLGTGLLLNYFSWQ
HHHHHCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
SIFYMFAGGALLMFVATCTIGSSRDETATPIDWVGAALIGTAIAVFVLGVVEAPVRGWTD
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PAVLGCLGAGVVLAGLFAVVQLRRAHPLLDVRLFRRPDFATGAAGITFLFIANFGFFYVA
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
MQFMQLVMGYSALETAFALSPLAFPVLILGGTLPLYLPKVGLRFAVTVGLLLLATGLFLM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RFLEADATFLDLMWPMLLAASGIGLCTAPTTSAIMNAVPDEKQGVASAVNDATREVGAAV
HHHHCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
GIAVAGSVLAAVYQSALAPKLGALPEQIRDAATDSLAHALAISEQMGPQGEQLADFARDA
HHHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
FMQAADQALFALSALLVVGAVFVAIWSPGRDGRQWAAIRRRRGADENRSAPAEVAP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVDTLPRTDQDIDAEIAALSKRKRIWLLVIASVDVLMVISSMVALNAALPDIALQTSATQ
CCCCCCCCCCCHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCH
SQLTWIVDGYTLALACLLLPAGAIGDRYGRRGALLVGLAIFAVASLAPVLFDSPMQIIIA
HHHHEEECHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
RAVAGVGAALIMPATLSLLTAAFPKSERNKAVGIWAGVAGSGAIFGFLGTGLLLNYFSWQ
HHHHHCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
SIFYMFAGGALLMFVATCTIGSSRDETATPIDWVGAALIGTAIAVFVLGVVEAPVRGWTD
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PAVLGCLGAGVVLAGLFAVVQLRRAHPLLDVRLFRRPDFATGAAGITFLFIANFGFFYVA
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
MQFMQLVMGYSALETAFALSPLAFPVLILGGTLPLYLPKVGLRFAVTVGLLLLATGLFLM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RFLEADATFLDLMWPMLLAASGIGLCTAPTTSAIMNAVPDEKQGVASAVNDATREVGAAV
HHHHCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
GIAVAGSVLAAVYQSALAPKLGALPEQIRDAATDSLAHALAISEQMGPQGEQLADFARDA
HHHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
FMQAADQALFALSALLVVGAVFVAIWSPGRDGRQWAAIRRRRGADENRSAPAEVAP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]