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The map label for this gene is tuf [H]

Identifier: 108797960

GI number: 108797960

Start: 1093263

End: 1094453

Strand: Direct

Name: tuf [H]

Synonym: Mmcs_0987

Alternate gene names: 108797960

Gene position: 1093263-1094453 (Clockwise)

Preceding gene: 108797959

Following gene: 108797961

Centisome position: 19.16

GC content: 65.83

Gene sequence:

>1191_bases
GTGGCGAAGGCGAAGTTCGAGCGGACGAAGCCGCACGTCAACATCGGGACCATCGGTCACGTTGACCACGGCAAGACCAC
GCTGACCGCAGCAATCACCAAGGTTCTGCACGACAAGTACCCCGAGTTGAACGAGTCGCGCGCATTCGACCAGATCGACA
ATGCGCCTGAGGAGCGTCAGCGCGGCATCACGATCAACATCTCCCACGTGGAGTACCAGACCGAGAAGCGTCACTACGCG
CACGTCGACGCCCCCGGTCACGCTGACTACATCAAGAACATGATCACCGGTGCCGCCCAGATGGACGGCGCGATCCTGGT
GGTCGCCGCGACCGACGGCCCGATGCCGCAGACCCGCGAGCACGTGCTGCTGGCCCGCCAGGTCGGCGTCCCCTACATCC
TGGTGGCGCTGAACAAGGCCGACGCGGTCGACGACGAGGAGCTCATCGAGCTCGTCGAGATGGAGGTCCGCGAACTGCTG
GCCGCCCAGGACTTCGACGAGGACGCCCCGGTCGTGCGCGTCTCGGCGCTCAAGGCGCTCGAGGGTGACGAGAAGTGGGT
CAAGTCGGTCGAGGAGCTCATGGACGCGGTCGACGAGTCGATCCCGGATCCGGTCCGCGACACCGACCGTCCGTTCCTGA
TGCCCGTCGAGGACGTCTTCACGATCACCGGCCGCGGCACCGTGGTCACCGGTCGTGTGGAGCGCGGCGTGGTCAACGTG
AACGAGGAAGTCGAGATCGTCGGCATCCGCCCCGGCACCACCAAGACCACGGTCACCGGTGTCGAGATGTTCCGCAAGCT
GCTCGACCAGGGCCAGGCCGGTGACAACGTCGGTCTGCTGCTGCGCGGTATCAAGCGTGAGGACGTCGAGCGCGGCCAGG
TCGTGGTCAAGCCCGGCACCACCACCCCGCACACCGAGTTCGACGGCCAGGTCTACATCCTGTCCAAGGACGAGGGTGGC
CGCCACACGCCGTTCTTCAACAACTACCGTCCGCAGTTCTACTTCCGGACCACGGACGTGACCGGCGTGGTGACCCTGCC
CGAGGGCACCGAGATGGTGATGCCCGGTGACAACACCGACATCTCCGTCAAGCTGATCCAGCCCGTCGCCATGGACGAGG
GCCTGCGGTTCGCCATCCGTGAGGGTGGCCGTACCGTCGGCGCCGGCCGCGTGACGAAGATCCACAAGTAA

Upstream 100 bases:

>100_bases
CAGTAGACCTGCGTATTCGCTGATCTGGTAAGACCACACAACTGAACAGATCAAACCTGCTTCCAAAGAAAGCACCAACA
CAGTCCAGGAGGACACCACA

Downstream 100 bases:

>100_bases
GACCAGTACGCCGAAGCGGCGCTCACCCTCTCGGGGGTGGGCGCCGCTTTGCGTTGTGCGATTTCGGCGTGCGGGGTTGC
GCTGAACGCGACTGCGCACG

Product: elongation factor Tu

Products: NA

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLHDKYPELNESRAFDQIDNAPEERQRGITINISHVEYQTEKRHYA
HVDAPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTREHVLLARQVGVPYILVALNKADAVDDEELIELVEMEVRELL
AAQDFDEDAPVVRVSALKALEGDEKWVKSVEELMDAVDESIPDPVRDTDRPFLMPVEDVFTITGRGTVVTGRVERGVVNV
NEEVEIVGIRPGTTKTTVTGVEMFRKLLDQGQAGDNVGLLLRGIKREDVERGQVVVKPGTTTPHTEFDGQVYILSKDEGG
RHTPFFNNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNTDISVKLIQPVAMDEGLRFAIREGGRTVGAGRVTKIHK

Sequences:

>Translated_396_residues
MAKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLHDKYPELNESRAFDQIDNAPEERQRGITINISHVEYQTEKRHYA
HVDAPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTREHVLLARQVGVPYILVALNKADAVDDEELIELVEMEVRELL
AAQDFDEDAPVVRVSALKALEGDEKWVKSVEELMDAVDESIPDPVRDTDRPFLMPVEDVFTITGRGTVVTGRVERGVVNV
NEEVEIVGIRPGTTKTTVTGVEMFRKLLDQGQAGDNVGLLLRGIKREDVERGQVVVKPGTTTPHTEFDGQVYILSKDEGG
RHTPFFNNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNTDISVKLIQPVAMDEGLRFAIREGGRTVGAGRVTKIHK
>Mature_395_residues
AKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLHDKYPELNESRAFDQIDNAPEERQRGITINISHVEYQTEKRHYAH
VDAPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTREHVLLARQVGVPYILVALNKADAVDDEELIELVEMEVRELLA
AQDFDEDAPVVRVSALKALEGDEKWVKSVEELMDAVDESIPDPVRDTDRPFLMPVEDVFTITGRGTVVTGRVERGVVNVN
EEVEIVGIRPGTTKTTVTGVEMFRKLLDQGQAGDNVGLLLRGIKREDVERGQVVVKPGTTTPHTEFDGQVYILSKDEGGR
HTPFFNNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNTDISVKLIQPVAMDEGLRFAIREGGRTVGAGRVTKIHK

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=56.0913705583756, Blast_Score=452, Evalue=1e-127,
Organism=Homo sapiens, GI4503471, Length=445, Percent_Identity=30.561797752809, Blast_Score=165, Evalue=6e-41,
Organism=Homo sapiens, GI4503475, Length=445, Percent_Identity=29.8876404494382, Blast_Score=161, Evalue=8e-40,
Organism=Homo sapiens, GI223555963, Length=441, Percent_Identity=31.0657596371882, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI5729864, Length=441, Percent_Identity=31.0657596371882, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI194018522, Length=337, Percent_Identity=29.673590504451, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI194097354, Length=337, Percent_Identity=29.673590504451, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI194018520, Length=337, Percent_Identity=29.673590504451, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI46094014, Length=338, Percent_Identity=29.8816568047337, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI54607086, Length=323, Percent_Identity=27.8637770897833, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI4503507, Length=307, Percent_Identity=26.3843648208469, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI157426893, Length=235, Percent_Identity=26.8085106382979, Blast_Score=66, Evalue=6e-11,
Organism=Homo sapiens, GI53729339, Length=230, Percent_Identity=33.4782608695652, Blast_Score=65, Evalue=1e-10,
Organism=Homo sapiens, GI53729337, Length=230, Percent_Identity=33.4782608695652, Blast_Score=65, Evalue=1e-10,
Organism=Escherichia coli, GI1790412, Length=395, Percent_Identity=74.6835443037975, Blast_Score=575, Evalue=1e-165,
Organism=Escherichia coli, GI1789737, Length=395, Percent_Identity=74.6835443037975, Blast_Score=575, Evalue=1e-165,
Organism=Escherichia coli, GI2367247, Length=339, Percent_Identity=31.5634218289086, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI48994988, Length=284, Percent_Identity=32.0422535211268, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789108, Length=337, Percent_Identity=27.299703264095, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788922, Length=147, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=401, Percent_Identity=49.1271820448878, Blast_Score=363, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI25141371, Length=374, Percent_Identity=44.9197860962567, Blast_Score=317, Evalue=9e-87,
Organism=Caenorhabditis elegans, GI17552884, Length=446, Percent_Identity=30.4932735426009, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17569207, Length=446, Percent_Identity=30.4932735426009, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI32566303, Length=429, Percent_Identity=28.2051282051282, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI115532067, Length=436, Percent_Identity=28.2110091743119, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI115532065, Length=436, Percent_Identity=28.2110091743119, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17506081, Length=273, Percent_Identity=31.5018315018315, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI32566629, Length=350, Percent_Identity=28, Blast_Score=121, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI17509919, Length=296, Percent_Identity=27.3648648648649, Blast_Score=95, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI32566301, Length=148, Percent_Identity=41.8918918918919, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17557151, Length=232, Percent_Identity=26.2931034482759, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324761, Length=398, Percent_Identity=62.3115577889447, Blast_Score=488, Evalue=1e-139,
Organism=Saccharomyces cerevisiae, GI6325337, Length=444, Percent_Identity=30.4054054054054, Blast_Score=165, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6319594, Length=444, Percent_Identity=30.4054054054054, Blast_Score=165, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6322937, Length=353, Percent_Identity=26.628895184136, Blast_Score=103, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6320377, Length=345, Percent_Identity=28.1159420289855, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=282, Percent_Identity=27.3049645390071, Blast_Score=80, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6323320, Length=239, Percent_Identity=28.4518828451883, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=58.7786259541985, Blast_Score=465, Evalue=1e-131,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=58.7786259541985, Blast_Score=465, Evalue=1e-131,
Organism=Drosophila melanogaster, GI19921738, Length=389, Percent_Identity=50.3856041131105, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI45553807, Length=445, Percent_Identity=31.0112359550562, Blast_Score=165, Evalue=4e-41,
Organism=Drosophila melanogaster, GI45553816, Length=445, Percent_Identity=31.0112359550562, Blast_Score=165, Evalue=4e-41,
Organism=Drosophila melanogaster, GI24651721, Length=445, Percent_Identity=31.0112359550562, Blast_Score=165, Evalue=4e-41,
Organism=Drosophila melanogaster, GI17864154, Length=445, Percent_Identity=31.0112359550562, Blast_Score=165, Evalue=4e-41,
Organism=Drosophila melanogaster, GI24652838, Length=446, Percent_Identity=29.5964125560538, Blast_Score=161, Evalue=6e-40,
Organism=Drosophila melanogaster, GI17137572, Length=446, Percent_Identity=29.5964125560538, Blast_Score=161, Evalue=6e-40,
Organism=Drosophila melanogaster, GI45550900, Length=339, Percent_Identity=31.858407079646, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI19922690, Length=305, Percent_Identity=30.4918032786885, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI17137380, Length=338, Percent_Identity=30.4733727810651, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24647046, Length=286, Percent_Identity=26.5734265734266, Blast_Score=88, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24647048, Length=286, Percent_Identity=26.5734265734266, Blast_Score=88, Evalue=9e-18,
Organism=Drosophila melanogaster, GI78706572, Length=249, Percent_Identity=30.5220883534137, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI28572034, Length=166, Percent_Identity=36.7469879518072, Blast_Score=68, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24582462, Length=163, Percent_Identity=31.9018404907975, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: NA

Molecular weight: Translated: 43764; Mature: 43633

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLHDKYPELNESRAFDQIDNAPEERQ
CCCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHH
RGITINISHVEYQTEKRHYAHVDAPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTRE
CCEEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEEECCCCCCCCHH
HVLLARQVGVPYILVALNKADAVDDEELIELVEMEVRELLAAQDFDEDAPVVRVSALKAL
HHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHC
EGDEKWVKSVEELMDAVDESIPDPVRDTDRPFLMPVEDVFTITGRGTVVTGRVERGVVNV
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHEEEEECCCEEEEEECCCCEEEC
NEEVEIVGIRPGTTKTTVTGVEMFRKLLDQGQAGDNVGLLLRGIKREDVERGQVVVKPGT
CCCEEEEEECCCCCCEEEHHHHHHHHHHHCCCCCCCHHHEEECCCCCCCCCCEEEEECCC
TTPHTEFDGQVYILSKDEGGRHTPFFNNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNTD
CCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCEEEECCCCCC
ISVKLIQPVAMDEGLRFAIREGGRTVGAGRVTKIHK
EEEEEECCCHHCCCCEEEECCCCCEECCCCEEEECC
>Mature Secondary Structure 
AKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLHDKYPELNESRAFDQIDNAPEERQ
CCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHH
RGITINISHVEYQTEKRHYAHVDAPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTRE
CCEEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEEECCCCCCCCHH
HVLLARQVGVPYILVALNKADAVDDEELIELVEMEVRELLAAQDFDEDAPVVRVSALKAL
HHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHC
EGDEKWVKSVEELMDAVDESIPDPVRDTDRPFLMPVEDVFTITGRGTVVTGRVERGVVNV
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHEEEEECCCEEEEEECCCCEEEC
NEEVEIVGIRPGTTKTTVTGVEMFRKLLDQGQAGDNVGLLLRGIKREDVERGQVVVKPGT
CCCEEEEEECCCCCCEEEHHHHHHHHHHHCCCCCCCHHHEEECCCCCCCCCCEEEEECCC
TTPHTEFDGQVYILSKDEGGRHTPFFNNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNTD
CCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCEEEECCCCCC
ISVKLIQPVAMDEGLRFAIREGGRTVGAGRVTKIHK
EEEEEECCCHHCCCCEEEECCCCCEECCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA