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The map label for this gene is 108797771

Identifier: 108797771

GI number: 108797771

Start: 853913

End: 855367

Strand: Direct

Name: 108797771

Synonym: Mmcs_0793

Alternate gene names: NA

Gene position: 853913-855367 (Clockwise)

Preceding gene: 108797770

Following gene: 108797772

Centisome position: 14.97

GC content: 70.58

Gene sequence:

>1455_bases
ATGACCACTCGATTGTCCGCCCTCGAGGCGACGGCGCTGTATACGCAGACCTCCACGACACCCGCGCACACGGTGTCGGT
GATCGTGCTCGACGCGTCCGAACGGCTCAGCCATGACGCGCTGGGTCAGTGGATCGCCGCGGGGTTACCGCGTTCGGCCC
GGTTCCGCAGCCGACTGGTCGGTAAACCCTTCGACGTGGGCCAGCCCGTGTGGGCGGAGATCGACGATTTCGACCCCGGC
CCGCAGATTCACCGCGCGACGGTGCCCGCCCCCGGAGACGGGCGCGACGTCGCGGCGCTCGTCACGAAACTCACCTCAGG
ACAACAGGATTGGCATCGATGGCTGTGGGAGGCCTGGACCATCGACGGTCTGGCGGGCGGCCGGTGGGCGCTGGCGGTGA
AGATGTCGCCGGTGTTGAGCGGTGCTTCGGCGGTTCGGGACTGCCTGTTGACCGGCGCACCGGGATCCGGGTACCGCACA
CCGATGGAGCCCGGTGCGGGCCCGCCCCCGTCGCTGGGTGAACTCGTCGCGGACACCCTGAGCGAGATGGTGGAGAACCA
GCTGACCGGGGCGTGGATGCTGACCGACGCGGTGGCGGGGACGTTGCCCGCGGTCCGCCGCGGACTCGCCGAGCTCGCAC
CGCCGGCCGGGCCCGCGTCCGTTTCCGTATTCGCCGCCCCGCTCACCAAACGGCGGGCCATGGCGTTCGTGTCGATCCCG
ATGGCCGAGGTCAAGACGGTCAGCGATGCGTTCGGCGGGAACACCGCGAACGTGCTGCTCGCCGCCTGCACGCTGTCGCT
GCGGGCCTGGCTGGAGCGCCACGATGCGGTGCCCGATGAGGCGCTGCTCATGGAGGTCCCGCTCTCGCTGCCTGCGGGTG
ATCCCGCGGAAGCGGGTGAGACGGTCGCGATGGGCAGGGTGCGCATGCCGGTCCACCTCGACGACCCGGTGCAGGTGCTC
ACCGACCTGCACACGGCGACGGAGAGGTTGAACATCGCACACAGTGACATCGGCGAAGATCTCGATGGCGCAGTCGATCT
CGCCTCGGTGGTTTCGCTGCTTCCGCCGTGGGCGATCCGCATGGGGGCGCGGGCCTACCGGAAACTGGGGATGTCCCGAC
TGCGCGCGACGACATGCCACGGCAGTGTCTCGTTCGCGGCAGGGCCGTCCGGTCCGGCGTTCTGCGCGGGCGCCGAGGTG
GTCGGGATGTTCACCGCCGAGCCCATGGTGGAGGGCCGCGGCCTGAACGTCACGGCGACCTCGCACGCCGAGGTGATGGA
CCTCTGCCTGACCGCGTGCCCGGACACCGTGCCGTGGGTCGACGACATCGCGCGGGGGATCGGCGACGCGCTGCAGCTGC
TGCTCGACGCGGCCGGGCGGTCACCGCGCGGAGAGGGCCGGTCGGTGGTCACGGAGATGACTTCCTACACCCCGAAGCGC
TCACGCCGGGCGTGA

Upstream 100 bases:

>100_bases
GATGTGAAAGACCGTGGTGTATCGGTTGGCCGGCGACTCCAGGACGGCCACCGCCGAAGGTGAAGATGAACAACTCCGAC
CCTGAGGGGCGTGAGCGCAG

Downstream 100 bases:

>100_bases
CCCCTGGGGGCGGACGGCGAACATCGGGAGGTTAGCTGCCCGAGATCAGCGGGTAACAGAACGGTATGCCGTTAGGGGAC
GGTACGAAGATCGCCGGGTA

Product: diacylglycerol O-acyltransferase

Products: NA

Alternate protein names: Putative triacylglycerol synthase Rv1425/MT1468 [H]

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MTTRLSALEATALYTQTSTTPAHTVSVIVLDASERLSHDALGQWIAAGLPRSARFRSRLVGKPFDVGQPVWAEIDDFDPG
PQIHRATVPAPGDGRDVAALVTKLTSGQQDWHRWLWEAWTIDGLAGGRWALAVKMSPVLSGASAVRDCLLTGAPGSGYRT
PMEPGAGPPPSLGELVADTLSEMVENQLTGAWMLTDAVAGTLPAVRRGLAELAPPAGPASVSVFAAPLTKRRAMAFVSIP
MAEVKTVSDAFGGNTANVLLAACTLSLRAWLERHDAVPDEALLMEVPLSLPAGDPAEAGETVAMGRVRMPVHLDDPVQVL
TDLHTATERLNIAHSDIGEDLDGAVDLASVVSLLPPWAIRMGARAYRKLGMSRLRATTCHGSVSFAAGPSGPAFCAGAEV
VGMFTAEPMVEGRGLNVTATSHAEVMDLCLTACPDTVPWVDDIARGIGDALQLLLDAAGRSPRGEGRSVVTEMTSYTPKR
SRRA

Sequences:

>Translated_484_residues
MTTRLSALEATALYTQTSTTPAHTVSVIVLDASERLSHDALGQWIAAGLPRSARFRSRLVGKPFDVGQPVWAEIDDFDPG
PQIHRATVPAPGDGRDVAALVTKLTSGQQDWHRWLWEAWTIDGLAGGRWALAVKMSPVLSGASAVRDCLLTGAPGSGYRT
PMEPGAGPPPSLGELVADTLSEMVENQLTGAWMLTDAVAGTLPAVRRGLAELAPPAGPASVSVFAAPLTKRRAMAFVSIP
MAEVKTVSDAFGGNTANVLLAACTLSLRAWLERHDAVPDEALLMEVPLSLPAGDPAEAGETVAMGRVRMPVHLDDPVQVL
TDLHTATERLNIAHSDIGEDLDGAVDLASVVSLLPPWAIRMGARAYRKLGMSRLRATTCHGSVSFAAGPSGPAFCAGAEV
VGMFTAEPMVEGRGLNVTATSHAEVMDLCLTACPDTVPWVDDIARGIGDALQLLLDAAGRSPRGEGRSVVTEMTSYTPKR
SRRA
>Mature_483_residues
TTRLSALEATALYTQTSTTPAHTVSVIVLDASERLSHDALGQWIAAGLPRSARFRSRLVGKPFDVGQPVWAEIDDFDPGP
QIHRATVPAPGDGRDVAALVTKLTSGQQDWHRWLWEAWTIDGLAGGRWALAVKMSPVLSGASAVRDCLLTGAPGSGYRTP
MEPGAGPPPSLGELVADTLSEMVENQLTGAWMLTDAVAGTLPAVRRGLAELAPPAGPASVSVFAAPLTKRRAMAFVSIPM
AEVKTVSDAFGGNTANVLLAACTLSLRAWLERHDAVPDEALLMEVPLSLPAGDPAEAGETVAMGRVRMPVHLDDPVQVLT
DLHTATERLNIAHSDIGEDLDGAVDLASVVSLLPPWAIRMGARAYRKLGMSRLRATTCHGSVSFAAGPSGPAFCAGAEVV
GMFTAEPMVEGRGLNVTATSHAEVMDLCLTACPDTVPWVDDIARGIGDALQLLLDAAGRSPRGEGRSVVTEMTSYTPKRS
RRA

Specific function: Upon expression in E.coli functions very weakly as a triacylglycerol synthase, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Has no wax synthase activity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the long-chain O-acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014292
- InterPro:   IPR004255 [H]

Pfam domain/function: PF03007 UPF0089 [H]

EC number: =2.3.1.20 [H]

Molecular weight: Translated: 50947; Mature: 50815

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTRLSALEATALYTQTSTTPAHTVSVIVLDASERLSHDALGQWIAAGLPRSARFRSRLV
CCCCHHHHHHHHEEEECCCCCCCEEEEEEECCHHHHCHHHHHHHHHHCCCHHHHHHHHHC
GKPFDVGQPVWAEIDDFDPGPQIHRATVPAPGDGRDVAALVTKLTSGQQDWHRWLWEAWT
CCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHC
IDGLAGGRWALAVKMSPVLSGASAVRDCLLTGAPGSGYRTPMEPGAGPPPSLGELVADTL
CCCCCCCCEEEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
SEMVENQLTGAWMLTDAVAGTLPAVRRGLAELAPPAGPASVSVFAAPLTKRRAMAFVSIP
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHEECC
MAEVKTVSDAFGGNTANVLLAACTLSLRAWLERHDAVPDEALLMEVPLSLPAGDPAEAGE
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCHHCCC
TVAMGRVRMPVHLDDPVQVLTDLHTATERLNIAHSDIGEDLDGAVDLASVVSLLPPWAIR
EEEECEEECCEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHH
MGARAYRKLGMSRLRATTCHGSVSFAAGPSGPAFCAGAEVVGMFTAEPMVEGRGLNVTAT
HHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCEECCCCEEEEEECCCCCCCCCCEEEEC
SHAEVMDLCLTACPDTVPWVDDIARGIGDALQLLLDAAGRSPRGEGRSVVTEMTSYTPKR
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCC
SRRA
CCCC
>Mature Secondary Structure 
TTRLSALEATALYTQTSTTPAHTVSVIVLDASERLSHDALGQWIAAGLPRSARFRSRLV
CCCHHHHHHHHEEEECCCCCCCEEEEEEECCHHHHCHHHHHHHHHHCCCHHHHHHHHHC
GKPFDVGQPVWAEIDDFDPGPQIHRATVPAPGDGRDVAALVTKLTSGQQDWHRWLWEAWT
CCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHC
IDGLAGGRWALAVKMSPVLSGASAVRDCLLTGAPGSGYRTPMEPGAGPPPSLGELVADTL
CCCCCCCCEEEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
SEMVENQLTGAWMLTDAVAGTLPAVRRGLAELAPPAGPASVSVFAAPLTKRRAMAFVSIP
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHEECC
MAEVKTVSDAFGGNTANVLLAACTLSLRAWLERHDAVPDEALLMEVPLSLPAGDPAEAGE
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCHHCCC
TVAMGRVRMPVHLDDPVQVLTDLHTATERLNIAHSDIGEDLDGAVDLASVVSLLPPWAIR
EEEECEEECCEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHH
MGARAYRKLGMSRLRATTCHGSVSFAAGPSGPAFCAGAEVVGMFTAEPMVEGRGLNVTAT
HHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCEECCCCEEEEEECCCCCCCCCCEEEEC
SHAEVMDLCLTACPDTVPWVDDIARGIGDALQLLLDAAGRSPRGEGRSVVTEMTSYTPKR
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCC
SRRA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]