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The map label for this gene is menE [H]

Identifier: 108797705

GI number: 108797705

Start: 774876

End: 775940

Strand: Reverse

Name: menE [H]

Synonym: Mmcs_0726

Alternate gene names: 108797705

Gene position: 775940-774876 (Counterclockwise)

Preceding gene: 108797706

Following gene: 108797703

Centisome position: 13.6

GC content: 73.9

Gene sequence:

>1065_bases
ATGGCCGGCCGTGGCCCGGCGCTGCTGCCCACTCCGGCCGACGACGCCGACCACGCCGCGCGGTTGTCCGGGGCACTGCG
CGCCGGTGCGGCGATCGACGAGGACGTCGCCGTCGTGGTGCCGACCTCGGGGACCACCGGAACCCCAAAGGGGGCGATGC
TGACCGCCGCGGCGTTGACGGCGAGCGCGCATGCCACCCATGCGCGATTGGGTGGCCCCGGCCGGTGGTTGCTCGCGCTG
CCCGCCCACCACATCGCCGGACTGCAGGTGTTGGTGCGCAGTGTCGTCGCCGGCGAGCGGCCGGTCGCGATACCTGCGAG
TTTCGAGCCACCCGACCTCGTGTCGGCGGTGGCGGGTATGGGCGCGGGCCGGCGGTACGTGTCGTTGGTGAGCGTGCAAC
TGGACAAGGCGCTGCGCGATCCGGCGGCCACCGCGGCGCTCGCCACGTTCGACGCGGTGCTGATCGGCGGTGGCCCGATG
CCGGCCGGACTGGCTGAGAAAGCCTCTGCCGCAGCGGTTCCCGTCGTCCGGACATACGGGATGAGCGAGACGGCGGGCGG
CTGTGTCTACGACGGGGTGCCGCTGGACGGGGTGCGGGTGCGTCTCGAGGGATCCAGGGTCCTGCTGGGCGGGCCGACGC
TGGCCAAGGGCTACCGCAACACGGTGCAGCCGGACCCCTTCGCCGAACCGGGCTGGTTCCGCACCGACGACGTCGGCGCG
CTGGACGACGGTGTGTTGCGGGTGCTGGGCCGTGTCGACGACGCCGTCAGCACCGGCGGGCTGACGGTGATGCCCCCTGT
CGTCGAGGCGGTGTTGTCCCGCCACCCGGCTGTCGCCGACTGTGCGGTGTTCGGGGTGGCCGACGAGCGACTCGGGCAGC
GGGTGGTCGCGGCGATCGTGCTCGCCGCGGGCGCCCCGGCGCCGACGGTGGCGGACCTGCGCGCACACGTCGCGCGCGAA
TTGGATGCGACCGCCGCACCGCGCGAGGTGCACGTGGTGGACGAGGTGCCGCGAAGGGGGATCGGCAAGGTGGACCGCCG
CGCGCTGAGCGCGCAGTTCAGCTAG

Upstream 100 bases:

>100_bases
ACGACCGCGAACCCCGGGAGGACACATAGCCGTCCTGCGCGCCCTCGCCGTCCCGTCCGGCGCCGCGGCGCTCGACATCC
TCCCGGTCGTCGAGACCCTG

Downstream 100 bases:

>100_bases
CGCGCGCTGAGCGCGCAGTTCAGCTAGCGCGGGGGCTTGAGCGGATCGTGCCCGATCGTCATGAGCCGGTGCCGCCAGTT
CTCCTGCCCGGCCGTCGGCT

Product: O-succinylbenzoic acid--CoA ligase

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MAGRGPALLPTPADDADHAARLSGALRAGAAIDEDVAVVVPTSGTTGTPKGAMLTAAALTASAHATHARLGGPGRWLLAL
PAHHIAGLQVLVRSVVAGERPVAIPASFEPPDLVSAVAGMGAGRRYVSLVSVQLDKALRDPAATAALATFDAVLIGGGPM
PAGLAEKASAAAVPVVRTYGMSETAGGCVYDGVPLDGVRVRLEGSRVLLGGPTLAKGYRNTVQPDPFAEPGWFRTDDVGA
LDDGVLRVLGRVDDAVSTGGLTVMPPVVEAVLSRHPAVADCAVFGVADERLGQRVVAAIVLAAGAPAPTVADLRAHVARE
LDATAAPREVHVVDEVPRRGIGKVDRRALSAQFS

Sequences:

>Translated_354_residues
MAGRGPALLPTPADDADHAARLSGALRAGAAIDEDVAVVVPTSGTTGTPKGAMLTAAALTASAHATHARLGGPGRWLLAL
PAHHIAGLQVLVRSVVAGERPVAIPASFEPPDLVSAVAGMGAGRRYVSLVSVQLDKALRDPAATAALATFDAVLIGGGPM
PAGLAEKASAAAVPVVRTYGMSETAGGCVYDGVPLDGVRVRLEGSRVLLGGPTLAKGYRNTVQPDPFAEPGWFRTDDVGA
LDDGVLRVLGRVDDAVSTGGLTVMPPVVEAVLSRHPAVADCAVFGVADERLGQRVVAAIVLAAGAPAPTVADLRAHVARE
LDATAAPREVHVVDEVPRRGIGKVDRRALSAQFS
>Mature_353_residues
AGRGPALLPTPADDADHAARLSGALRAGAAIDEDVAVVVPTSGTTGTPKGAMLTAAALTASAHATHARLGGPGRWLLALP
AHHIAGLQVLVRSVVAGERPVAIPASFEPPDLVSAVAGMGAGRRYVSLVSVQLDKALRDPAATAALATFDAVLIGGGPMP
AGLAEKASAAAVPVVRTYGMSETAGGCVYDGVPLDGVRVRLEGSRVLLGGPTLAKGYRNTVQPDPFAEPGWFRTDDVGAL
DDGVLRVLGRVDDAVSTGGLTVMPPVVEAVLSRHPAVADCAVFGVADERLGQRVVAAIVLAAGAPAPTVADLRAHVAREL
DATAAPREVHVVDEVPRRGIGKVDRRALSAQFS

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA). May be involved in the biosynthesis of menaquinone [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=388, Percent_Identity=27.0618556701031, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI187761343, Length=388, Percent_Identity=27.0618556701031, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI122937307, Length=125, Percent_Identity=35.2, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI115511026, Length=125, Percent_Identity=34.4, Blast_Score=69, Evalue=9e-12,
Organism=Escherichia coli, GI1788595, Length=321, Percent_Identity=31.4641744548287, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI145693145, Length=358, Percent_Identity=27.6536312849162, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1788107, Length=350, Percent_Identity=28, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI1786810, Length=247, Percent_Identity=27.1255060728745, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1790505, Length=110, Percent_Identity=33.6363636363636, Blast_Score=65, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI32563687, Length=371, Percent_Identity=30.188679245283, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17558820, Length=407, Percent_Identity=25.7985257985258, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17531443, Length=359, Percent_Identity=26.1838440111421, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17557194, Length=359, Percent_Identity=25.3481894150418, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17538037, Length=361, Percent_Identity=26.0387811634349, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI71985884, Length=360, Percent_Identity=25.5555555555556, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17559526, Length=366, Percent_Identity=25.4098360655738, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17556356, Length=209, Percent_Identity=24.4019138755981, Blast_Score=65, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6319699, Length=363, Percent_Identity=25.6198347107438, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6319264, Length=130, Percent_Identity=36.1538461538462, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323182, Length=216, Percent_Identity=29.6296296296296, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI18859661, Length=376, Percent_Identity=29.5212765957447, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24581924, Length=381, Percent_Identity=27.0341207349081, Blast_Score=95, Evalue=7e-20,
Organism=Drosophila melanogaster, GI21355181, Length=359, Percent_Identity=25.6267409470752, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21356947, Length=337, Percent_Identity=28.1899109792285, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24653035, Length=347, Percent_Identity=24.7838616714697, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 35878; Mature: 35747

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGRGPALLPTPADDADHAARLSGALRAGAAIDEDVAVVVPTSGTTGTPKGAMLTAAALT
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHH
ASAHATHARLGGPGRWLLALPAHHIAGLQVLVRSVVAGERPVAIPASFEPPDLVSAVAGM
HHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHCC
GAGRRYVSLVSVQLDKALRDPAATAALATFDAVLIGGGPMPAGLAEKASAAAVPVVRTYG
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHEEEECCCCCCCCHHHHHCCCCCHHHHHCC
MSETAGGCVYDGVPLDGVRVRLEGSRVLLGGPTLAKGYRNTVQPDPFAEPGWFRTDDVGA
CCCCCCCEEECCCCCCCEEEEEECCEEEEECCHHHHCCCCCCCCCCCCCCCCCCCCCCCC
LDDGVLRVLGRVDDAVSTGGLTVMPPVVEAVLSRHPAVADCAVFGVADERLGQRVVAAIV
CHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHCCCCHHHHHEECCCHHHHHHHHHHHHH
LAAGAPAPTVADLRAHVARELDATAAPREVHVVDEVPRRGIGKVDRRALSAQFS
HHCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEHHHHCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure 
AGRGPALLPTPADDADHAARLSGALRAGAAIDEDVAVVVPTSGTTGTPKGAMLTAAALT
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHH
ASAHATHARLGGPGRWLLALPAHHIAGLQVLVRSVVAGERPVAIPASFEPPDLVSAVAGM
HHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHCC
GAGRRYVSLVSVQLDKALRDPAATAALATFDAVLIGGGPMPAGLAEKASAAAVPVVRTYG
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHEEEECCCCCCCCHHHHHCCCCCHHHHHCC
MSETAGGCVYDGVPLDGVRVRLEGSRVLLGGPTLAKGYRNTVQPDPFAEPGWFRTDDVGA
CCCCCCCEEECCCCCCCEEEEEECCEEEEECCHHHHCCCCCCCCCCCCCCCCCCCCCCCC
LDDGVLRVLGRVDDAVSTGGLTVMPPVVEAVLSRHPAVADCAVFGVADERLGQRVVAAIV
CHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHCCCCHHHHHEECCCHHHHHHHHHHHHH
LAAGAPAPTVADLRAHVARELDATAAPREVHVVDEVPRRGIGKVDRRALSAQFS
HHCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEHHHHCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]