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The map label for this gene is def

Identifier: 108797549

GI number: 108797549

Start: 631598

End: 632191

Strand: Reverse

Name: def

Synonym: Mmcs_0569

Alternate gene names: 108797549

Gene position: 632191-631598 (Counterclockwise)

Preceding gene: 108797556

Following gene: 108797548

Centisome position: 11.08

GC content: 70.2

Gene sequence:

>594_bases
GTGGCAGTCGTTCCGATCCGCATCGTGGGAGATCCAGTCCTGCGCACCGAAACCACCCCGATCCCCGTCGGCGATGACGG
TTCCCTCCCCGCCGAGGTGGCCGATCTCATCCGCGATCTGTACGAGACGATGGACGCCGCCAACGGTGTCGGCTTGGCCG
CCAACCAGATCGGCGTCTCCCAGCGGGTGTTCGTCTACGACTGCCCCGATTCGCGCGGCCGGGCGGGCCGGCGCCGCGGG
GTCGTGATCAATCCGGTGCTGGAGACGTCCGACATCCCCGAGACCATGCCCGATCCCGATGACGACGAAGAGGGCTGCCT
GTCGGTGCCCGGCGAGCAGTTCCCCACCGGGCGGGCCGACTGGGCGCGGGTGACGGGCCTGGACGCCGACGGCAGCCCCA
TCACCGTCGAGGGCACCGGACTGTTCGCGCGGATGCTGCAGCACGAGACGGGCCACCTCGACGGATTCCTCTACCTCGAC
CGCCTGATCGGTCGGCACGCGCGCGCGGCCAAACGCGCGGTCAAACACAACGGCTGGGGGGTGCCCGGGCTGAGTTGGAC
GCCGGGCGAGGATCCCGATCCGTTCGGCCACTGA

Upstream 100 bases:

>100_bases
CATGTGGTTCGGCGCTCATTCAATCACGGCGGCCAGAGTTGAGGCGTCACCGATACGGGCGAGTCGGCTCACATAAGCGG
CTCGCCCTAAGCTCCTCCAC

Downstream 100 bases:

>100_bases
GCCGTCCCGTGCCCGAGCTACCCCGCGCCGGCACCCGGGTGAGCCTGCGTTACCGGCTGCCCGCCGGTTCGGTCCCGCCA
CTCAACGATGTCGTCGGACA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 197; Mature: 196

Protein sequence:

>197_residues
MAVVPIRIVGDPVLRTETTPIPVGDDGSLPAEVADLIRDLYETMDAANGVGLAANQIGVSQRVFVYDCPDSRGRAGRRRG
VVINPVLETSDIPETMPDPDDDEEGCLSVPGEQFPTGRADWARVTGLDADGSPITVEGTGLFARMLQHETGHLDGFLYLD
RLIGRHARAAKRAVKHNGWGVPGLSWTPGEDPDPFGH

Sequences:

>Translated_197_residues
MAVVPIRIVGDPVLRTETTPIPVGDDGSLPAEVADLIRDLYETMDAANGVGLAANQIGVSQRVFVYDCPDSRGRAGRRRG
VVINPVLETSDIPETMPDPDDDEEGCLSVPGEQFPTGRADWARVTGLDADGSPITVEGTGLFARMLQHETGHLDGFLYLD
RLIGRHARAAKRAVKHNGWGVPGLSWTPGEDPDPFGH
>Mature_196_residues
AVVPIRIVGDPVLRTETTPIPVGDDGSLPAEVADLIRDLYETMDAANGVGLAANQIGVSQRVFVYDCPDSRGRAGRRRGV
VINPVLETSDIPETMPDPDDDEEGCLSVPGEQFPTGRADWARVTGLDADGSPITVEGTGLFARMLQHETGHLDGFLYLDR
LIGRHARAAKRAVKHNGWGVPGLSWTPGEDPDPFGH

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=170, Percent_Identity=32.9411764705882, Blast_Score=75, Evalue=5e-14,
Organism=Escherichia coli, GI1789682, Length=163, Percent_Identity=37.4233128834356, Blast_Score=99, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24645728, Length=166, Percent_Identity=30.7228915662651, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24645726, Length=166, Percent_Identity=30.7228915662651, Blast_Score=73, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_MYCSJ (A3PTZ4)

Other databases:

- EMBL:   CP000580
- RefSeq:   YP_001068862.1
- ProteinModelPortal:   A3PTZ4
- SMR:   A3PTZ4
- STRING:   A3PTZ4
- EnsemblBacteria:   EBMYCT00000049824
- GeneID:   4876304
- GenomeReviews:   CP000580_GR
- KEGG:   mjl:Mjls_0559
- eggNOG:   COG0242
- GeneTree:   EBGT00050000016783
- HOGENOM:   HBG665227
- OMA:   FVYDCAD
- ProtClustDB:   PRK00150
- BioCyc:   MSP164757:MJLS_0559-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 21146; Mature: 21015

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: NA

Important sites: ACT_SITE 149-149

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVVPIRIVGDPVLRTETTPIPVGDDGSLPAEVADLIRDLYETMDAANGVGLAANQIGVS
CCEEEEEECCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEHHCCCCC
QRVFVYDCPDSRGRAGRRRGVVINPVLETSDIPETMPDPDDDEEGCLSVPGEQFPTGRAD
CEEEEEECCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCC
WARVTGLDADGSPITVEGTGLFARMLQHETGHLDGFLYLDRLIGRHARAAKRAVKHNGWG
CCEEECCCCCCCEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
VPGLSWTPGEDPDPFGH
CCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AVVPIRIVGDPVLRTETTPIPVGDDGSLPAEVADLIRDLYETMDAANGVGLAANQIGVS
CEEEEEECCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEHHCCCCC
QRVFVYDCPDSRGRAGRRRGVVINPVLETSDIPETMPDPDDDEEGCLSVPGEQFPTGRAD
CEEEEEECCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCC
WARVTGLDADGSPITVEGTGLFARMLQHETGHLDGFLYLDRLIGRHARAAKRAVKHNGWG
CCEEECCCCCCCEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
VPGLSWTPGEDPDPFGH
CCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA