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The map label for this gene is xthA [H]

Identifier: 108797547

GI number: 108797547

Start: 629892

End: 630695

Strand: Reverse

Name: xthA [H]

Synonym: Mmcs_0567

Alternate gene names: 108797547

Gene position: 630695-629892 (Counterclockwise)

Preceding gene: 108797548

Following gene: 108797544

Centisome position: 11.05

GC content: 68.16

Gene sequence:

>804_bases
ATGCGCCTGGCCACCTGGAACGTCAACTCGATCCGCGCCCGGGTGGACCGGGTCACCGATTGGCTGGAGCGGGCCGACGT
CGACGTGCTGGCCATGCAGGAGACCAAGTGCAACGACGAGCAGTTCCCCACCATGCCGTTCGCCGCGCTCGGCTACGACG
TCGTGCACTGCGGGTTCAACCAGTGGAACGGCGTCGCGATCGCATCGCGGGTCGGCCTCGACGACGTGGCGGTCGGCTTC
GACGGCCAACCCACCTGGGGTGACAAACCCGACATAGAGGCCGCCGCCGAGGCCCGCGCGTTGGGTGCGACGTGCGCCGG
GGTGCGGGTGTGGAGCCTCTACGTACCCAACGGCCGGTTCGTCGGCTCACCGCACTACGCCTACAAGCTCGAATGGCTTG
CCGCCCTGCGCAAGACCGCGCACGGCTGGCTCACCGAGAACCCCGCCCTGCCGATCGCGATGGTCGGCGACTGGAACATC
GCCCCGACCGACGACGACGTCTGGAGCGTCGAGGCGTATCAGGACAGTACCCACGTCACACCGCCCGAGCGTCAGGCCTT
CACCGAGATCGTCGACGCCGGATACGCCGACGTGGTGCGACCTTTCACCCCCGGACCGGGTGTCTACACGTACTGGGACT
ACACCCGGCTGGCGTTCCAGAAGCGGCGCGGTATGCGCATCGACTTCATCCTGGGCTCACCGGCGTTCGCCGATCGGGTC
ACCCACGCCGAGATCGTCCGCGAGGAACGCAAGGGCAAGGGCGCCAGCGACCACGCACCGGTGTTGGTGGACCTGCGAGA
CTGA

Upstream 100 bases:

>100_bases
GGTGCTCGCCGACAACCACGGCGCCATCGCACTCTACGAGTCGATGGGGTTCACGGCACAGCACCGGGAGCGCTACGTCG
ACGCTTCTAAGCTGTAGCCC

Downstream 100 bases:

>100_bases
ATCTCCCTGTCAGGATTCGGCGTTGAGCACCCTGGCGAGCTCGCCGCGGGAACGGATGCCCAGCCTGCGGTAGACGCGCG
ACAGGTTGGCCTCCACCGTC

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MRLATWNVNSIRARVDRVTDWLERADVDVLAMQETKCNDEQFPTMPFAALGYDVVHCGFNQWNGVAIASRVGLDDVAVGF
DGQPTWGDKPDIEAAAEARALGATCAGVRVWSLYVPNGRFVGSPHYAYKLEWLAALRKTAHGWLTENPALPIAMVGDWNI
APTDDDVWSVEAYQDSTHVTPPERQAFTEIVDAGYADVVRPFTPGPGVYTYWDYTRLAFQKRRGMRIDFILGSPAFADRV
THAEIVREERKGKGASDHAPVLVDLRD

Sequences:

>Translated_267_residues
MRLATWNVNSIRARVDRVTDWLERADVDVLAMQETKCNDEQFPTMPFAALGYDVVHCGFNQWNGVAIASRVGLDDVAVGF
DGQPTWGDKPDIEAAAEARALGATCAGVRVWSLYVPNGRFVGSPHYAYKLEWLAALRKTAHGWLTENPALPIAMVGDWNI
APTDDDVWSVEAYQDSTHVTPPERQAFTEIVDAGYADVVRPFTPGPGVYTYWDYTRLAFQKRRGMRIDFILGSPAFADRV
THAEIVREERKGKGASDHAPVLVDLRD
>Mature_267_residues
MRLATWNVNSIRARVDRVTDWLERADVDVLAMQETKCNDEQFPTMPFAALGYDVVHCGFNQWNGVAIASRVGLDDVAVGF
DGQPTWGDKPDIEAAAEARALGATCAGVRVWSLYVPNGRFVGSPHYAYKLEWLAALRKTAHGWLTENPALPIAMVGDWNI
APTDDDVWSVEAYQDSTHVTPPERQAFTEIVDAGYADVVRPFTPGPGVYTYWDYTRLAFQKRRGMRIDFILGSPAFADRV
THAEIVREERKGKGASDHAPVLVDLRD

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=273, Percent_Identity=28.5714285714286, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI18375503, Length=273, Percent_Identity=28.5714285714286, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI18375501, Length=273, Percent_Identity=28.5714285714286, Blast_Score=99, Evalue=4e-21,
Organism=Escherichia coli, GI1788046, Length=274, Percent_Identity=28.4671532846715, Blast_Score=105, Evalue=4e-24,
Organism=Drosophila melanogaster, GI221330655, Length=270, Percent_Identity=27.4074074074074, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI17136678, Length=270, Percent_Identity=27.7777777777778, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29741; Mature: 29741

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLATWNVNSIRARVDRVTDWLERADVDVLAMQETKCNDEQFPTMPFAALGYDVVHCGFN
CEEEECCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHCCEEEEECCC
QWNGVAIASRVGLDDVAVGFDGQPTWGDKPDIEAAAEARALGATCAGVRVWSLYVPNGRF
CCCCEEEEEECCCCCEEECCCCCCCCCCCCCHHHHHHHHHHCCEECCEEEEEEECCCCEE
VGSPHYAYKLEWLAALRKTAHGWLTENPALPIAMVGDWNIAPTDDDVWSVEAYQDSTHVT
ECCCCCEEHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCEEEEEEECCCCCCC
PPERQAFTEIVDAGYADVVRPFTPGPGVYTYWDYTRLAFQKRRGMRIDFILGSPAFADRV
CCHHHHHHHHHHCCHHHHHCCCCCCCCEEEEHHHHHHHHHHHCCCEEEEEECCCHHHHHH
THAEIVREERKGKGASDHAPVLVDLRD
HHHHHHHHHHCCCCCCCCCCEEEEECC
>Mature Secondary Structure
MRLATWNVNSIRARVDRVTDWLERADVDVLAMQETKCNDEQFPTMPFAALGYDVVHCGFN
CEEEECCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHCCEEEEECCC
QWNGVAIASRVGLDDVAVGFDGQPTWGDKPDIEAAAEARALGATCAGVRVWSLYVPNGRF
CCCCEEEEEECCCCCEEECCCCCCCCCCCCCHHHHHHHHHHCCEECCEEEEEEECCCCEE
VGSPHYAYKLEWLAALRKTAHGWLTENPALPIAMVGDWNIAPTDDDVWSVEAYQDSTHVT
ECCCCCEEHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCEEEEEEECCCCCCC
PPERQAFTEIVDAGYADVVRPFTPGPGVYTYWDYTRLAFQKRRGMRIDFILGSPAFADRV
CCHHHHHHHHHHCCHHHHHCCCCCCCCEEEEHHHHHHHHHHHCCCEEEEEECCCHHHHHH
THAEIVREERKGKGASDHAPVLVDLRD
HHHHHHHHHHCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]