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The map label for this gene is trmH [C]

Identifier: 108797462

GI number: 108797462

Start: 536889

End: 537533

Strand: Direct

Name: trmH [C]

Synonym: Mmcs_0482

Alternate gene names: 108797462

Gene position: 536889-537533 (Clockwise)

Preceding gene: 108797461

Following gene: 108797465

Centisome position: 9.41

GC content: 68.99

Gene sequence:

>645_bases
GTGACTGAGGCGCCGGGTCCCACCGAATGGGGTGAGGCCCCCGGCGTGGGACCGTGGCCGGGGGACTTGCCCGACGATCC
GCGGTACGACCCGGAACTGTTGCGCGACGGCGACACCCGCAACGTCGTCGACGCGTACCGCTACTGGACGCGTGAGGCGA
TCATCGCCGACATCGACCGGCGCAGGCACCCGCTGCACATCGCGATCGAGAACTTCGGCAACGATGCGAACATCGGCTCG
GTGGTGCGCACCGCCAACGCCTTCGCCGTCGACACGGTGCACATCGTGGGCCGGCGCCGCTGGAATCGCCGGGGCGCCAT
GGTCACCGACCGCTACCAGCGGCTGCGCCATCACGACAGCACCGCCGAACTGCTCGACTTCGCAGCCGAAGCCGGGCTGA
CCGCGGTCGCGGTCGACAACATCCCCGGCGCCGTACCGCTGGAACACACCGATCTGCCGCGCGAGTGTCTGCTGATCTTC
GGGCAGGAGGGGCCCGGGATCACCGAAGACGCGAAAACCGGTGCACGGCTGACGGTCTCAATTGCCGCGTTCGGCTCCAC
ACGCAGTATCAACGCGGCCGTCGCCGCCGGCATCGCCATGCACACCTGGATCGTTCAGCACGCCGACCTCGGCAAGGCGT
GGTAG

Upstream 100 bases:

>100_bases
AACATGCGCGCCTCCCTGTGTACGCAGGGTGAGATCAACCGCGCGCTGCTGTTCGCCTCGGTGTCGGTGGTCGGGACCCA
CGCCGCACTGTGGACCACCG

Downstream 100 bases:

>100_bases
CCGCTCACGCAGCAGCACCCACACCGTCACCGCCGAGGTCAGCATCAGCGCCGCGCCGACACCGGAGGCGACCGCCAGTC
CGCTGCTGAAGGATTCGCGC

Product: tRNA/rRNA methyltransferase SpoU

Products: S-adenosyl-L-homocysteine; tRNATyr Containing 2'-O-methylguanosine [C]

Alternate protein names: RNA Methyltransferase; rRNA Methylase; TRNA/RRNA MethyltransferaU; RNA Methyltransferase TrmH Family; 23S RRNA Methyltransferase; TrmH Family RNA Methyltransferase; RNA Methylase Family Protein; Spou RRNA Methylase Family Protein; Methyltransferase; SPOU RRNA Methylase Family Protein; TRNA/RRNA Methyltransferase Family Protein; tRNA Guanosine-2-O-Methyltransferase

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MTEAPGPTEWGEAPGVGPWPGDLPDDPRYDPELLRDGDTRNVVDAYRYWTREAIIADIDRRRHPLHIAIENFGNDANIGS
VVRTANAFAVDTVHIVGRRRWNRRGAMVTDRYQRLRHHDSTAELLDFAAEAGLTAVAVDNIPGAVPLEHTDLPRECLLIF
GQEGPGITEDAKTGARLTVSIAAFGSTRSINAAVAAGIAMHTWIVQHADLGKAW

Sequences:

>Translated_214_residues
MTEAPGPTEWGEAPGVGPWPGDLPDDPRYDPELLRDGDTRNVVDAYRYWTREAIIADIDRRRHPLHIAIENFGNDANIGS
VVRTANAFAVDTVHIVGRRRWNRRGAMVTDRYQRLRHHDSTAELLDFAAEAGLTAVAVDNIPGAVPLEHTDLPRECLLIF
GQEGPGITEDAKTGARLTVSIAAFGSTRSINAAVAAGIAMHTWIVQHADLGKAW
>Mature_213_residues
TEAPGPTEWGEAPGVGPWPGDLPDDPRYDPELLRDGDTRNVVDAYRYWTREAIIADIDRRRHPLHIAIENFGNDANIGSV
VRTANAFAVDTVHIVGRRRWNRRGAMVTDRYQRLRHHDSTAELLDFAAEAGLTAVAVDNIPGAVPLEHTDLPRECLLIFG
QEGPGITEDAKTGARLTVSIAAFGSTRSINAAVAAGIAMHTWIVQHADLGKAW

Specific function: Specifically Methylates Guanosine-18 In Various tRNAs. [C]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790083, Length=156, Percent_Identity=26.9230769230769, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.1.1.34 [C]

Molecular weight: Translated: 23431; Mature: 23300

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEAPGPTEWGEAPGVGPWPGDLPDDPRYDPELLRDGDTRNVVDAYRYWTREAIIADIDR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHC
RRHPLHIAIENFGNDANIGSVVRTANAFAVDTVHIVGRRRWNRRGAMVTDRYQRLRHHDS
CCCCEEEEEECCCCCCCHHHHHHHCCCHHHHHHHEEHHHHHCCCCCCHHHHHHHHHCCCC
TAELLDFAAEAGLTAVAVDNIPGAVPLEHTDLPRECLLIFGQEGPGITEDAKTGARLTVS
HHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCHHHEEEECCCCCCCCCCCCCCCEEEEE
IAAFGSTRSINAAVAAGIAMHTWIVQHADLGKAW
EEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TEAPGPTEWGEAPGVGPWPGDLPDDPRYDPELLRDGDTRNVVDAYRYWTREAIIADIDR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHC
RRHPLHIAIENFGNDANIGSVVRTANAFAVDTVHIVGRRRWNRRGAMVTDRYQRLRHHDS
CCCCEEEEEECCCCCCCHHHHHHHCCCHHHHHHHEEHHHHHCCCCCCHHHHHHHHHCCCC
TAELLDFAAEAGLTAVAVDNIPGAVPLEHTDLPRECLLIFGQEGPGITEDAKTGARLTVS
HHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCHHHEEEECCCCCCCCCCCCCCCEEEEE
IAAFGSTRSINAAVAAGIAMHTWIVQHADLGKAW
EEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; tRNATyr [C]

Specific reaction: S-Adenosyl-L-methionine + tRNATyr = S-adenosyl-L-homocysteine + tRNATyr Containing 2'-O-methylguanosine [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA