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The map label for this gene is cysNC [H]

Identifier: 108797367

GI number: 108797367

Start: 433658

End: 435517

Strand: Reverse

Name: cysNC [H]

Synonym: Mmcs_0387

Alternate gene names: 108797367

Gene position: 435517-433658 (Counterclockwise)

Preceding gene: 108797368

Following gene: 108797365

Centisome position: 7.63

GC content: 69.03

Gene sequence:

>1860_bases
ATGGGGACTCGTCAGTTACTGCGCATCACCACCGCGGGATCGGTCGACGACGGTAAGAGCACGCTGATCGGCAGGCTGTT
GCACGACACCGACAGCCTGCCGCTCGACCACCTCGAGGCCGTCACCGACGAGGAGGGGGTCGCCGACCTCGCCGCGCTCT
CCGACGGGCTGCGGGCCGAACGCGAACAGGGCATCACGATCGACGTCGCCTACCGGTTCTTCTCCACCGCCAACCGCAGC
TACATCCTGGCCGACACCCCGGGTCACGAGCGCTACACCCGCAACATGTTCACCGGCGCCTCCAATGCGCATGTGGCGGT
GCTGCTCGTCGACGCCCGCGCCGGGGTGCTCCGGCAGACCAACCGGCACGCGCGCATCGCGAAGCTGTTGGGTATCAAGC
ACTTCGTCGCGGCGGTGAACAAGATCGACCTCGTCGACTTCGACGGCGACCGCTTCGCCGAGGTGGCCGCGGAACTGCAG
ACGCTCTCGGCACGCCTGGGCGGTCTCGACATCACGGTCATCCCGCTCGCGGCCAAACACGGCGACAACGTCGTGCACCG
CTCCGACCGCACACCCTGGTACGACGGACCGACCCTGCTCGAGTACCTCGAAGGCGTGGAACTCGTTCCGCCGCAACCTG
AGCCGAACAAGCTGCGGCTGCCCGTGCAGTGGGTGTCGCGGCCGACCGCCGAACAGCGCCGCCGCTACACCGGCAGGTTG
TCGGCGGGCACGCTGAGCGTCGGTGACACGGTGGTGAGCCTTCCGGCCGGCACCCGCTCGACAGTCACGGTCGTCGACAC
CCTCGACGACGACCGGCCGGTCGCCGTGGCGCCGCTGTCGGTCGCGATCGAACTGGCCGACGACATCGACGTCGGCCGTG
GGGACGTGTTCGTCAGCGGCGCCGACGATGCGACGCTGCCGGTGCTCGCTCGCGAACTCGACGCGACGGTCTGCTGGTTC
GCCGACGCCCCGCTGCGCGCCGGTGACCGGCTGGCCCTCAAACAGGGCACCCGCACGGTGCGCGCCACGGTGCAGGCGCT
GCACAGCCGCCTGGACCCGGAGACCCTCGACGAGTTCGACAACCCGGTGGAGTTGGTCCTCAACGACATCGGATCGCTCA
CGCTGCGCACCAGTTCGGTGGTCGTGACCGACGCGTACGCCGACAACCGGGACAGCGGTGCGTTCATCCTGATCGACGAA
TCGAGCAACGACACCGTCGGCGCGGGCACCATCCTCGAACCACGTGAGGTCACGCCCGGGGCGCAGACCCGCAACGACAT
CCGTTGGCATCCCTCCTCACTCGACCGGACCCACCGCTGGACCTCGACGGGTCAGCGGGGTGCCACCATCTGGTTCACGG
GTCTGCCCGCATCGGGCAAGTCGACCGTCGCCGTGGCGGTCGAACGCGCGCTCGTCGAATCGGGGCAGGTGGCCTACCTG
CTCGATGGCGACAACATCCGTCACGGCCTGTCCGACGACCTGGGGTTCGCCGCGGGTGACCGCGCCGAGAACATCCGCCG
GGTCGGGCATCTCACCCGGCTGTTCGCCGATGCGGGTGTCGTCGCGCTGGCGTCGCTGGTGTCGCCGCTGAAATCCGATC
GGGAGATCGCGCGCACCCTCAGCGACGCCGCCAAGCTGCCGTTCCTCGAGGTGTACGTGTCCACCCCCGTCGAGGAATGC
GAAAAGCGGGACCCCAAGGGGCTTTACGCCCGCGCCCGCGCCGGCGAACTGAAGGGCCTCACGGGTGTCGACGCCCCCTA
CGAGCCGCCGGATGCTCCGGACCTGGTGCTCGACACCACCGGTGCCGACATCGCCGATCTGGTGAAGCAGGTGATCGACC
TGCTCGACAGCAGGCGTTAA

Upstream 100 bases:

>100_bases
AAATCTCGGCGGCGACGGTGTCCGAGCGCGGCGAGACCCGCGCCGACGACCGCACCTCGGTGGCGGCCATGGAGGACCGG
AAACGCGAGGGGTACTTCTG

Downstream 100 bases:

>100_bases
CCGGTCCGCGCGACCACGAACACCCGCCGGAACGGGAAGAAGGTCCGCCCGTCGGCGCGCACCGGGTAGGCCTCGTCGAG
CAGCGGGATCAGCTCGGCGC

Product: adenylylsulfate kinase / sulfate adenylyltransferase subunit 1

Products: NA

Alternate protein names: Sulfate adenylyltransferase subunit 1; ATP-sulfurylase large subunit; Sulfate adenylate transferase; SAT; Adenylyl-sulfate kinase; APS kinase; ATP adenosine-5'-phosphosulfate 3'-phosphotransferase [H]

Number of amino acids: Translated: 619; Mature: 618

Protein sequence:

>619_residues
MGTRQLLRITTAGSVDDGKSTLIGRLLHDTDSLPLDHLEAVTDEEGVADLAALSDGLRAEREQGITIDVAYRFFSTANRS
YILADTPGHERYTRNMFTGASNAHVAVLLVDARAGVLRQTNRHARIAKLLGIKHFVAAVNKIDLVDFDGDRFAEVAAELQ
TLSARLGGLDITVIPLAAKHGDNVVHRSDRTPWYDGPTLLEYLEGVELVPPQPEPNKLRLPVQWVSRPTAEQRRRYTGRL
SAGTLSVGDTVVSLPAGTRSTVTVVDTLDDDRPVAVAPLSVAIELADDIDVGRGDVFVSGADDATLPVLARELDATVCWF
ADAPLRAGDRLALKQGTRTVRATVQALHSRLDPETLDEFDNPVELVLNDIGSLTLRTSSVVVTDAYADNRDSGAFILIDE
SSNDTVGAGTILEPREVTPGAQTRNDIRWHPSSLDRTHRWTSTGQRGATIWFTGLPASGKSTVAVAVERALVESGQVAYL
LDGDNIRHGLSDDLGFAAGDRAENIRRVGHLTRLFADAGVVALASLVSPLKSDREIARTLSDAAKLPFLEVYVSTPVEEC
EKRDPKGLYARARAGELKGLTGVDAPYEPPDAPDLVLDTTGADIADLVKQVIDLLDSRR

Sequences:

>Translated_619_residues
MGTRQLLRITTAGSVDDGKSTLIGRLLHDTDSLPLDHLEAVTDEEGVADLAALSDGLRAEREQGITIDVAYRFFSTANRS
YILADTPGHERYTRNMFTGASNAHVAVLLVDARAGVLRQTNRHARIAKLLGIKHFVAAVNKIDLVDFDGDRFAEVAAELQ
TLSARLGGLDITVIPLAAKHGDNVVHRSDRTPWYDGPTLLEYLEGVELVPPQPEPNKLRLPVQWVSRPTAEQRRRYTGRL
SAGTLSVGDTVVSLPAGTRSTVTVVDTLDDDRPVAVAPLSVAIELADDIDVGRGDVFVSGADDATLPVLARELDATVCWF
ADAPLRAGDRLALKQGTRTVRATVQALHSRLDPETLDEFDNPVELVLNDIGSLTLRTSSVVVTDAYADNRDSGAFILIDE
SSNDTVGAGTILEPREVTPGAQTRNDIRWHPSSLDRTHRWTSTGQRGATIWFTGLPASGKSTVAVAVERALVESGQVAYL
LDGDNIRHGLSDDLGFAAGDRAENIRRVGHLTRLFADAGVVALASLVSPLKSDREIARTLSDAAKLPFLEVYVSTPVEEC
EKRDPKGLYARARAGELKGLTGVDAPYEPPDAPDLVLDTTGADIADLVKQVIDLLDSRR
>Mature_618_residues
GTRQLLRITTAGSVDDGKSTLIGRLLHDTDSLPLDHLEAVTDEEGVADLAALSDGLRAEREQGITIDVAYRFFSTANRSY
ILADTPGHERYTRNMFTGASNAHVAVLLVDARAGVLRQTNRHARIAKLLGIKHFVAAVNKIDLVDFDGDRFAEVAAELQT
LSARLGGLDITVIPLAAKHGDNVVHRSDRTPWYDGPTLLEYLEGVELVPPQPEPNKLRLPVQWVSRPTAEQRRRYTGRLS
AGTLSVGDTVVSLPAGTRSTVTVVDTLDDDRPVAVAPLSVAIELADDIDVGRGDVFVSGADDATLPVLARELDATVCWFA
DAPLRAGDRLALKQGTRTVRATVQALHSRLDPETLDEFDNPVELVLNDIGSLTLRTSSVVVTDAYADNRDSGAFILIDES
SNDTVGAGTILEPREVTPGAQTRNDIRWHPSSLDRTHRWTSTGQRGATIWFTGLPASGKSTVAVAVERALVESGQVAYLL
DGDNIRHGLSDDLGFAAGDRAENIRRVGHLTRLFADAGVVALASLVSPLKSDREIARTLSDAAKLPFLEVYVSTPVEECE
KRDPKGLYARARAGELKGLTGVDAPYEPPDAPDLVLDTTGADIADLVKQVIDLLDSRR

Specific function: APS kinase catalyzes the synthesis of activated sulfate [H]

COG id: COG2895

COG function: function code P; GTPases - Sulfate adenylate transferase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the APS kinase family [H]

Homologues:

Organism=Homo sapiens, GI46094058, Length=203, Percent_Identity=48.2758620689655, Blast_Score=209, Evalue=9e-54,
Organism=Homo sapiens, GI34447231, Length=208, Percent_Identity=45.6730769230769, Blast_Score=204, Evalue=2e-52,
Organism=Homo sapiens, GI62912492, Length=208, Percent_Identity=45.6730769230769, Blast_Score=204, Evalue=3e-52,
Organism=Homo sapiens, GI223555963, Length=440, Percent_Identity=29.5454545454545, Blast_Score=153, Evalue=4e-37,
Organism=Homo sapiens, GI5729864, Length=440, Percent_Identity=29.7727272727273, Blast_Score=153, Evalue=4e-37,
Organism=Homo sapiens, GI4503471, Length=458, Percent_Identity=27.292576419214, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI194018522, Length=440, Percent_Identity=25.9090909090909, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI4503475, Length=458, Percent_Identity=26.8558951965066, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI194018520, Length=440, Percent_Identity=25.9090909090909, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI194097354, Length=440, Percent_Identity=25.9090909090909, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI46094014, Length=440, Percent_Identity=25.9090909090909, Blast_Score=129, Evalue=9e-30,
Organism=Homo sapiens, GI34147630, Length=275, Percent_Identity=29.4545454545455, Blast_Score=81, Evalue=3e-15,
Organism=Escherichia coli, GI1789108, Length=424, Percent_Identity=47.1698113207547, Blast_Score=342, Evalue=3e-95,
Organism=Escherichia coli, GI1789107, Length=190, Percent_Identity=47.3684210526316, Blast_Score=187, Evalue=2e-48,
Organism=Escherichia coli, GI1790412, Length=145, Percent_Identity=31.0344827586207, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1789737, Length=145, Percent_Identity=31.0344827586207, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI2367247, Length=174, Percent_Identity=31.6091954022989, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17542422, Length=223, Percent_Identity=42.6008968609865, Blast_Score=198, Evalue=8e-51,
Organism=Caenorhabditis elegans, GI115532067, Length=283, Percent_Identity=33.5689045936396, Blast_Score=145, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI115532065, Length=283, Percent_Identity=33.5689045936396, Blast_Score=145, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI17552884, Length=467, Percent_Identity=28.2655246252677, Blast_Score=142, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI17569207, Length=467, Percent_Identity=28.2655246252677, Blast_Score=142, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI32566629, Length=288, Percent_Identity=30.5555555555556, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI32566303, Length=460, Percent_Identity=26.5217391304348, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI32566301, Length=150, Percent_Identity=38, Blast_Score=88, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI25141371, Length=261, Percent_Identity=29.8850574712644, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17556456, Length=144, Percent_Identity=32.6388888888889, Blast_Score=70, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6322852, Length=194, Percent_Identity=46.9072164948454, Blast_Score=184, Evalue=4e-47,
Organism=Saccharomyces cerevisiae, GI6325337, Length=438, Percent_Identity=27.8538812785388, Blast_Score=141, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6319594, Length=438, Percent_Identity=27.8538812785388, Blast_Score=141, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6322937, Length=280, Percent_Identity=28.2142857142857, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6320377, Length=444, Percent_Identity=25.4504504504505, Blast_Score=104, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6324761, Length=246, Percent_Identity=31.7073170731707, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24667032, Length=174, Percent_Identity=50, Blast_Score=185, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24667028, Length=174, Percent_Identity=50, Blast_Score=185, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24667036, Length=174, Percent_Identity=50, Blast_Score=185, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24667040, Length=174, Percent_Identity=50, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI116007838, Length=174, Percent_Identity=50, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24667044, Length=174, Percent_Identity=50, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24652838, Length=348, Percent_Identity=31.6091954022989, Blast_Score=138, Evalue=1e-32,
Organism=Drosophila melanogaster, GI17137572, Length=348, Percent_Identity=31.6091954022989, Blast_Score=138, Evalue=1e-32,
Organism=Drosophila melanogaster, GI45553807, Length=296, Percent_Identity=31.7567567567568, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI45553816, Length=296, Percent_Identity=31.7567567567568, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24651721, Length=296, Percent_Identity=31.7567567567568, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI17864154, Length=296, Percent_Identity=31.7567567567568, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI45550900, Length=429, Percent_Identity=26.5734265734266, Blast_Score=122, Evalue=7e-28,
Organism=Drosophila melanogaster, GI17137380, Length=446, Percent_Identity=26.2331838565022, Blast_Score=119, Evalue=9e-27,
Organism=Drosophila melanogaster, GI281363316, Length=278, Percent_Identity=30.5755395683453, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI17864358, Length=278, Percent_Identity=30.5755395683453, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI19921738, Length=219, Percent_Identity=30.1369863013699, Blast_Score=86, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002891
- InterPro:   IPR000795
- InterPro:   IPR011779
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF01583 APS_kinase; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: =2.7.7.4; =2.7.1.25 [H]

Molecular weight: Translated: 66803; Mature: 66671

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTRQLLRITTAGSVDDGKSTLIGRLLHDTDSLPLDHLEAVTDEEGVADLAALSDGLRAE
CCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHH
REQGITIDVAYRFFSTANRSYILADTPGHERYTRNMFTGASNAHVAVLLVDARAGVLRQT
HHCCCEEEEEEHHHHCCCCCEEEECCCCCHHHHHHHHCCCCCCEEEEEEEECCCCHHHHC
NRHARIAKLLGIKHFVAAVNKIDLVDFDGDRFAEVAAELQTLSARLGGLDITVIPLAAKH
CCHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEECC
GDNVVHRSDRTPWYDGPTLLEYLEGVELVPPQPEPNKLRLPVQWVSRPTAEQRRRYTGRL
CCCCEECCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCEEEEHHHHCCCCHHHHHHHCCCC
SAGTLSVGDTVVSLPAGTRSTVTVVDTLDDDRPVAVAPLSVAIELADDIDVGRGDVFVSG
CCCEEECCCEEEECCCCCCCEEEEEEECCCCCCEEEECCEEEEEECCCCCCCCCCEEEEC
ADDATLPVLARELDATVCWFADAPLRAGDRLALKQGTRTVRATVQALHSRLDPETLDEFD
CCCCHHHHHHHHHCCEEEEEECCCCCCCCEEEHHHCCHHHHHHHHHHHHHCCHHHHHHCC
NPVELVLNDIGSLTLRTSSVVVTDAYADNRDSGAFILIDESSNDTVGAGTILEPREVTPG
CHHHHHHHHHCCEEEEECEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCEECCCCCCCC
AQTRNDIRWHPSSLDRTHRWTSTGQRGATIWFTGLPASGKSTVAVAVERALVESGQVAYL
CCCCCCCCCCCCCCCHHHCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEE
LDGDNIRHGLSDDLGFAAGDRAENIRRVGHLTRLFADAGVVALASLVSPLKSDREIARTL
ECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SDAAKLPFLEVYVSTPVEECEKRDPKGLYARARAGELKGLTGVDAPYEPPDAPDLVLDTT
HHHHCCCEEEEEECCCHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCEEEECC
GADIADLVKQVIDLLDSRR
CCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GTRQLLRITTAGSVDDGKSTLIGRLLHDTDSLPLDHLEAVTDEEGVADLAALSDGLRAE
CCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHH
REQGITIDVAYRFFSTANRSYILADTPGHERYTRNMFTGASNAHVAVLLVDARAGVLRQT
HHCCCEEEEEEHHHHCCCCCEEEECCCCCHHHHHHHHCCCCCCEEEEEEEECCCCHHHHC
NRHARIAKLLGIKHFVAAVNKIDLVDFDGDRFAEVAAELQTLSARLGGLDITVIPLAAKH
CCHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEECC
GDNVVHRSDRTPWYDGPTLLEYLEGVELVPPQPEPNKLRLPVQWVSRPTAEQRRRYTGRL
CCCCEECCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCEEEEHHHHCCCCHHHHHHHCCCC
SAGTLSVGDTVVSLPAGTRSTVTVVDTLDDDRPVAVAPLSVAIELADDIDVGRGDVFVSG
CCCEEECCCEEEECCCCCCCEEEEEEECCCCCCEEEECCEEEEEECCCCCCCCCCEEEEC
ADDATLPVLARELDATVCWFADAPLRAGDRLALKQGTRTVRATVQALHSRLDPETLDEFD
CCCCHHHHHHHHHCCEEEEEECCCCCCCCEEEHHHCCHHHHHHHHHHHHHCCHHHHHHCC
NPVELVLNDIGSLTLRTSSVVVTDAYADNRDSGAFILIDESSNDTVGAGTILEPREVTPG
CHHHHHHHHHCCEEEEECEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCEECCCCCCCC
AQTRNDIRWHPSSLDRTHRWTSTGQRGATIWFTGLPASGKSTVAVAVERALVESGQVAYL
CCCCCCCCCCCCCCCHHHCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEE
LDGDNIRHGLSDDLGFAAGDRAENIRRVGHLTRLFADAGVVALASLVSPLKSDREIARTL
ECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SDAAKLPFLEVYVSTPVEECEKRDPKGLYARARAGELKGLTGVDAPYEPPDAPDLVLDTT
HHHHCCCEEEEEECCCHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCEEEECC
GADIADLVKQVIDLLDSRR
CCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]