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The map label for this gene is pyk [H]

Identifier: 108797363

GI number: 108797363

Start: 429257

End: 431104

Strand: Direct

Name: pyk [H]

Synonym: Mmcs_0383

Alternate gene names: 108797363

Gene position: 429257-431104 (Clockwise)

Preceding gene: 108797361

Following gene: 108797366

Centisome position: 7.52

GC content: 69.32

Gene sequence:

>1848_bases
GTGTCGTCTGCGCATACCCCGGTCGTGTCACGGCTGGAAACCGCCGACCGCGCACTCGAACAGCTCGACGAGCTGATCGA
ACGGCTCGAGAAGGCCGAAGACGCGTGGTCGGACTGGCTCACCGCGGTGAGCCCCGAGCACCGCGCCAGCGCGACGAACA
TGGTCCACTACTGGTCGCTGCGACAGAGCGATCTGCGTGACCTCCAGCGCCGGCTGGCGGGTCTGGGCCTGTCGTCGCTG
GGCCGCAGCGAACCACACGTGGAGGGGACGTTGCGGCTCGTCCACGCGGCGGTGGCCGCAATGCGTCACGGCTCCTGGCA
TCCGCCCGGCGTCACCGCCGTCGACGCGAACGTCGGCGACGAACTGCTGCGGGAGCACGCCGTTGACCTGTTCGGCCCGG
CACCGGCCGAGCGCGCAACCCGCATCATGGTGACCCTGCCCTCGTCGGCCGCGACCGACCCGGATCTGGTGCGCGACCTC
ATCGCACGCGGGATGAACGTGGCCCGCATCAACTGCGCACACGACGATGCCGAAGCCTGGACCGCGATGGCCGGCCACGT
CCGGCGCGCCGCGGAGTCGACCGGTCGAAAGTGCCTTGTCGCGATGGACCTCGCGGGCCCGAAACTGCGCACCGGACCGA
TCCGGCCCGGGCCGCGGGTGATCAAGCTGCGACCCACGCGCGATGCGCTCGGCCGCGTGGTGACACCCTTTCGGCTGCGG
CTCACCGCATCTGAAGAGCCCACTGGCTCAAGCGAATCCGCAATGGCTGTGGTGCCGGTCGATGAGGCGTGGCTGGCCCG
CCGCCGCGACGGGGACGTGGTCGGATTCCACGACGCCCGCGGGGCCAAGCGGCAACTCGTCCTGAGCCGTCCCGACGAGA
TGGCCGGGGCCGTCATCGCGACCGGCGACAAGACGAGCTACCTCGCCACCGGAACCGTTCTGCACGCCGGTGCGCACGAC
CCGTGCGAGGTCGGCCTGCTTCCCGAACGGGAGCAGACGCTCATGGTGCAGCGGGGCGACGAGTTGACGCTCACGCGCGA
CTGTGCGCCGGTTCCCGCCGACCACGGAGGGGCGCCGCGGATCGGGTGCACCCTGCCCGAGGTCTTCGACCACGCGCGGC
CCGGCGAGAAGATCCGGTTCGACGACGGCCGCATCGGGGGTGAGATCGTCGCCGTCGAACGCGATGCGCTGCGGGTACGG
ATCGACCGCACCGCACCCGGCGGGTCGAAGCTCGGTTCGGCCAAAGGCGTCAACGTGCCGGACACCCACCTGCCGATCGC
GGCGCTGACCGACAAGGATGTGGAGGATCTCGCGACGGTCGTCGCGATCGCCGACATCGTCCAGATCTCTTTCGTGCAGC
GGCCTTCCGACATCACGCAGCTGCACGACGAACTGCACCGGCTCGGTGGCGACCACCTCGGTGTGGTGCTCAAGATCGAG
ACCCGGCGGGCGTTCGAACACCTCCCGCAACTGCTCCTCACCGCGATGCGGTGGCCGCGCGTCGGTGTGATGATCGCCCG
CGGTGATCTCGCGGTGGAGGTCGGTTACGAACGGCTCGCCGAGGTGCAGGAAGAGGTTCTGTGGTTGTGCGAAGCGGCGC
ACCTTCCGGTCATCTGGGCCACCCAGGTGCTCGAGAGCCTGGCCAAGTCGGGTCTGCCGTCGCGTGCCGAGATCAGCGAC
GCCGCCATGGGTGAGCGTGCGGAGTGCGTCATGCTCAACAAAGGTCCGCACATCGTCGACGCGGTCGTGGTGCTCGACGA
CATCCTGCGTCGAATGAACGAACACCACTACAAGAAGAACGCACTGCTCCGGCAGCTGCGGTCGTGGCGGCCCGACGCGA
CGGAATGA

Upstream 100 bases:

>100_bases
TGGCTCGCGATCATGCTGTCTCGCGGCCCCCGGCGTTCACCGGGAGGTAACCCGCGGCCGTTAGCGTGGCGAACTCCGAC
AGAGAACCAGGAGTGCCGCC

Downstream 100 bases:

>100_bases
CGTGCGCTAGGCGACGAAGCCGAACCGACGGCCGGTCACCCGGTCGGGCCTGATCCGCACGAAGTGCTGTTTGGTCGTCG
CCGTCCACGAAAAAAGCTGC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 615; Mature: 614

Protein sequence:

>615_residues
MSSAHTPVVSRLETADRALEQLDELIERLEKAEDAWSDWLTAVSPEHRASATNMVHYWSLRQSDLRDLQRRLAGLGLSSL
GRSEPHVEGTLRLVHAAVAAMRHGSWHPPGVTAVDANVGDELLREHAVDLFGPAPAERATRIMVTLPSSAATDPDLVRDL
IARGMNVARINCAHDDAEAWTAMAGHVRRAAESTGRKCLVAMDLAGPKLRTGPIRPGPRVIKLRPTRDALGRVVTPFRLR
LTASEEPTGSSESAMAVVPVDEAWLARRRDGDVVGFHDARGAKRQLVLSRPDEMAGAVIATGDKTSYLATGTVLHAGAHD
PCEVGLLPEREQTLMVQRGDELTLTRDCAPVPADHGGAPRIGCTLPEVFDHARPGEKIRFDDGRIGGEIVAVERDALRVR
IDRTAPGGSKLGSAKGVNVPDTHLPIAALTDKDVEDLATVVAIADIVQISFVQRPSDITQLHDELHRLGGDHLGVVLKIE
TRRAFEHLPQLLLTAMRWPRVGVMIARGDLAVEVGYERLAEVQEEVLWLCEAAHLPVIWATQVLESLAKSGLPSRAEISD
AAMGERAECVMLNKGPHIVDAVVVLDDILRRMNEHHYKKNALLRQLRSWRPDATE

Sequences:

>Translated_615_residues
MSSAHTPVVSRLETADRALEQLDELIERLEKAEDAWSDWLTAVSPEHRASATNMVHYWSLRQSDLRDLQRRLAGLGLSSL
GRSEPHVEGTLRLVHAAVAAMRHGSWHPPGVTAVDANVGDELLREHAVDLFGPAPAERATRIMVTLPSSAATDPDLVRDL
IARGMNVARINCAHDDAEAWTAMAGHVRRAAESTGRKCLVAMDLAGPKLRTGPIRPGPRVIKLRPTRDALGRVVTPFRLR
LTASEEPTGSSESAMAVVPVDEAWLARRRDGDVVGFHDARGAKRQLVLSRPDEMAGAVIATGDKTSYLATGTVLHAGAHD
PCEVGLLPEREQTLMVQRGDELTLTRDCAPVPADHGGAPRIGCTLPEVFDHARPGEKIRFDDGRIGGEIVAVERDALRVR
IDRTAPGGSKLGSAKGVNVPDTHLPIAALTDKDVEDLATVVAIADIVQISFVQRPSDITQLHDELHRLGGDHLGVVLKIE
TRRAFEHLPQLLLTAMRWPRVGVMIARGDLAVEVGYERLAEVQEEVLWLCEAAHLPVIWATQVLESLAKSGLPSRAEISD
AAMGERAECVMLNKGPHIVDAVVVLDDILRRMNEHHYKKNALLRQLRSWRPDATE
>Mature_614_residues
SSAHTPVVSRLETADRALEQLDELIERLEKAEDAWSDWLTAVSPEHRASATNMVHYWSLRQSDLRDLQRRLAGLGLSSLG
RSEPHVEGTLRLVHAAVAAMRHGSWHPPGVTAVDANVGDELLREHAVDLFGPAPAERATRIMVTLPSSAATDPDLVRDLI
ARGMNVARINCAHDDAEAWTAMAGHVRRAAESTGRKCLVAMDLAGPKLRTGPIRPGPRVIKLRPTRDALGRVVTPFRLRL
TASEEPTGSSESAMAVVPVDEAWLARRRDGDVVGFHDARGAKRQLVLSRPDEMAGAVIATGDKTSYLATGTVLHAGAHDP
CEVGLLPEREQTLMVQRGDELTLTRDCAPVPADHGGAPRIGCTLPEVFDHARPGEKIRFDDGRIGGEIVAVERDALRVRI
DRTAPGGSKLGSAKGVNVPDTHLPIAALTDKDVEDLATVVAIADIVQISFVQRPSDITQLHDELHRLGGDHLGVVLKIET
RRAFEHLPQLLLTAMRWPRVGVMIARGDLAVEVGYERLAEVQEEVLWLCEAAHLPVIWATQVLESLAKSGLPSRAEISDA
AMGERAECVMLNKGPHIVDAVVVLDDILRRMNEHHYKKNALLRQLRSWRPDATE

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=262, Percent_Identity=32.4427480916031, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI32967597, Length=298, Percent_Identity=31.2080536912752, Blast_Score=106, Evalue=5e-23,
Organism=Homo sapiens, GI10835121, Length=298, Percent_Identity=31.2080536912752, Blast_Score=106, Evalue=6e-23,
Organism=Homo sapiens, GI33286422, Length=227, Percent_Identity=33.4801762114537, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI33286420, Length=227, Percent_Identity=33.4801762114537, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI310128732, Length=184, Percent_Identity=32.6086956521739, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI310128730, Length=184, Percent_Identity=32.6086956521739, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1787965, Length=263, Percent_Identity=29.277566539924, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1788160, Length=234, Percent_Identity=29.0598290598291, Blast_Score=77, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17544584, Length=234, Percent_Identity=33.7606837606838, Blast_Score=112, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17506831, Length=260, Percent_Identity=31.1538461538462, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17506829, Length=269, Percent_Identity=30.4832713754647, Blast_Score=93, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI71984413, Length=269, Percent_Identity=30.4832713754647, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI71984406, Length=269, Percent_Identity=30.4832713754647, Blast_Score=92, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6319279, Length=219, Percent_Identity=33.7899543378995, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6324923, Length=220, Percent_Identity=33.1818181818182, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI28571814, Length=257, Percent_Identity=29.5719844357977, Blast_Score=111, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24648964, Length=257, Percent_Identity=29.5719844357977, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24648966, Length=326, Percent_Identity=26.6871165644172, Blast_Score=93, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24581235, Length=245, Percent_Identity=25.7142857142857, Blast_Score=86, Evalue=8e-17,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 67130; Mature: 66999

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSAHTPVVSRLETADRALEQLDELIERLEKAEDAWSDWLTAVSPEHRASATNMVHYWSL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCEEEEECC
RQSDLRDLQRRLAGLGLSSLGRSEPHVEGTLRLVHAAVAAMRHGSWHPPGVTAVDANVGD
CHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCH
ELLREHAVDLFGPAPAERATRIMVTLPSSAATDPDLVRDLIARGMNVARINCAHDDAEAW
HHHHHHHHHHCCCCCCHHCCEEEEECCCCCCCCHHHHHHHHHCCCCEEEEEECCCCHHHH
TAMAGHVRRAAESTGRKCLVAMDLAGPKLRTGPIRPGPRVIKLRPTRDALGRVVTPFRLR
HHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCEEEEEECCHHHHHCCCCCEEEE
LTASEEPTGSSESAMAVVPVDEAWLARRRDGDVVGFHDARGAKRQLVLSRPDEMAGAVIA
EECCCCCCCCCCCEEEEEECCHHHHHHCCCCCEEEECCCCCCCCEEEECCCHHHCCEEEE
TGDKTSYLATGTVLHAGAHDPCEVGLLPEREQTLMVQRGDELTLTRDCAPVPADHGGAPR
CCCCCCEEEECCEEECCCCCCCCCCCCCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCC
IGCTLPEVFDHARPGEKIRFDDGRIGGEIVAVERDALRVRIDRTAPGGSKLGSAKGVNVP
CCCCCHHHHHCCCCCCEEEECCCCCCCEEEEEECCEEEEEEECCCCCCCCCCCCCCCCCC
DTHLPIAALTDKDVEDLATVVAIADIVQISFVQRPSDITQLHDELHRLGGDHLGVVLKIE
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEEEE
TRRAFEHLPQLLLTAMRWPRVGVMIARGDLAVEVGYERLAEVQEEVLWLCEAAHLPVIWA
HHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHCCCCHHHH
TQVLESLAKSGLPSRAEISDAAMGERAECVMLNKGPHIVDAVVVLDDILRRMNEHHYKKN
HHHHHHHHHCCCCCCCHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
ALLRQLRSWRPDATE
HHHHHHHHCCCCCCC
>Mature Secondary Structure 
SSAHTPVVSRLETADRALEQLDELIERLEKAEDAWSDWLTAVSPEHRASATNMVHYWSL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCEEEEECC
RQSDLRDLQRRLAGLGLSSLGRSEPHVEGTLRLVHAAVAAMRHGSWHPPGVTAVDANVGD
CHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCH
ELLREHAVDLFGPAPAERATRIMVTLPSSAATDPDLVRDLIARGMNVARINCAHDDAEAW
HHHHHHHHHHCCCCCCHHCCEEEEECCCCCCCCHHHHHHHHHCCCCEEEEEECCCCHHHH
TAMAGHVRRAAESTGRKCLVAMDLAGPKLRTGPIRPGPRVIKLRPTRDALGRVVTPFRLR
HHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCEEEEEECCHHHHHCCCCCEEEE
LTASEEPTGSSESAMAVVPVDEAWLARRRDGDVVGFHDARGAKRQLVLSRPDEMAGAVIA
EECCCCCCCCCCCEEEEEECCHHHHHHCCCCCEEEECCCCCCCCEEEECCCHHHCCEEEE
TGDKTSYLATGTVLHAGAHDPCEVGLLPEREQTLMVQRGDELTLTRDCAPVPADHGGAPR
CCCCCCEEEECCEEECCCCCCCCCCCCCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCC
IGCTLPEVFDHARPGEKIRFDDGRIGGEIVAVERDALRVRIDRTAPGGSKLGSAKGVNVP
CCCCCHHHHHCCCCCCEEEECCCCCCCEEEEEECCEEEEEEECCCCCCCCCCCCCCCCCC
DTHLPIAALTDKDVEDLATVVAIADIVQISFVQRPSDITQLHDELHRLGGDHLGVVLKIE
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEEEE
TRRAFEHLPQLLLTAMRWPRVGVMIARGDLAVEVGYERLAEVQEEVLWLCEAAHLPVIWA
HHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHCCCCHHHH
TQVLESLAKSGLPSRAEISDAAMGERAECVMLNKGPHIVDAVVVLDDILRRMNEHHYKKN
HHHHHHHHHCCCCCCCHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
ALLRQLRSWRPDATE
HHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286; 9625784 [H]