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The map label for this gene is shiA [H]

Identifier: 108797305

GI number: 108797305

Start: 363271

End: 364644

Strand: Direct

Name: shiA [H]

Synonym: Mmcs_0325

Alternate gene names: 108797305

Gene position: 363271-364644 (Clockwise)

Preceding gene: 108797302

Following gene: 108797307

Centisome position: 6.37

GC content: 67.98

Gene sequence:

>1374_bases
ATGGGATTCCGGCCCACCGGAGCACCGGGGAACGCGAAGATCGGGGACATGGACGAGACGGCAACTCCTGATCCGGCGTT
GCTGCGCCGGGTCGCGATTTCCAGTCTGCTGGGCACCGCGATCGAGTACTACGACTTTCTCGTCTACGGGACGATGAGCG
CGCTCGTGTTCGGGATCATCTTCTTCCCCGAATCGGATCCCGCGGTGGCCACCATCGCCGCGTTCGGCACGCTCGCCGCG
GGTTACGTCGCCCGGCCGGTCGGCGGAATCCTGTTCGGACACTTCGGTGACCGGATCGGCCGCAAATCGATGCTGGTGCT
GACGATGACCCTGATGGGCGTCGCGAGCTTCCTCGTCGGCGTGCTGCCCACGTTCGCCGCGATCGGGGTCGCGGCACCGA
TCCTGCTGGTGGCACTGCGCGTGATCCAGGGGGTGGCGATCGGAGGGGAATGGGGCGGAGCCACCCTGATGGTCACCGAA
CACGCCGAACCGCACCGCCGCGGGGCCTGGAACGGTGTCATGCAGATGGGTTCGCCGATCGGCTCGCTGCTGTCGGTGGC
CGTCGTCACCGCCATCACCCTGATGCCGGACGAGTCGTTCATGTCCTGGGGTTGGCGGGTGCCGTTCCTGGTCAGCCTGC
TGCTGCTCGGCCTCGGGATCTACGTGCGGTTGTCCATCACGGAAAGCCCCGTGTTCGAGCAGGCGAAACGGGCCGACGAC
CGCCCGCAGGGCCCCCCGCTCGTCGAGGTGCTGCGCCGCCCCGGGAACCTGGTCCTCGCCTGCGCCGTCGGGATCGGGCC
GTTCGCGCTCACGGCGTTGATCAGCACCTACATGATCAGCTACGCGACGGCGATCGGGTACCACCGCACGGACGTGATGA
CCGCGCTGATCTTCACGTCGACGACGGCGCTGATCACCATTCCGCTCTTCTCGGCCCTGTCGGATCGGGTGGGGCGGCGG
GCGGTGATCGTCACCGGGGCGATCGGCATCGTCTGCTACGCATGGCCGTTCTACGCACTCGTCGACATGGGTTCGGTTCC
GCTGCTCACCGTCGCGATGGTGATCGCGCAGGTGCTGCAGTCGCTGATGTACGCCCCGCTGGGCGCGCTGTTCTCGGAGA
TGTTCGGTACGCGGGTGCGCTACACCGGCGCCTCGATGGGCTACCAGTTCGCCGCCCTGTTGGGCGCCGGCTTCACACCG
TTGATCGCCAGCAGCCTGTTGGCAGAAGGGGTCTCGCGAACTCCATTGGTGGTACTGGCCGCGGGTTGCGGTGTGGTGAC
CATCGTCGCGATCTGGCGCGTCAGCGAGACGCGGGGCGCCGACCTCACCGACGCGGGGAGGCTACCCCGCGGAGCGGCGG
CGGCCGATCGCTAG

Upstream 100 bases:

>100_bases
GCTGCGACGACCCTGTCTCGACGGGTCACGGCCGTGATCGTGCTGTCCACCGCTGCAGGCTAGAGCCGTCCGGCGGACGT
GCACAGCGTTCTCTCGATGA

Downstream 100 bases:

>100_bases
TTGCGGAACCGCTTCAGGTAGGACACCAGTCGGCGCGCGGGCACCGGTTTCGGCCAGTCGTCGGTGCGGAAGTGGGCGTC
GGTGCCACCGTCGACGAAGA

Product: major facilitator transporter

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MGFRPTGAPGNAKIGDMDETATPDPALLRRVAISSLLGTAIEYYDFLVYGTMSALVFGIIFFPESDPAVATIAAFGTLAA
GYVARPVGGILFGHFGDRIGRKSMLVLTMTLMGVASFLVGVLPTFAAIGVAAPILLVALRVIQGVAIGGEWGGATLMVTE
HAEPHRRGAWNGVMQMGSPIGSLLSVAVVTAITLMPDESFMSWGWRVPFLVSLLLLGLGIYVRLSITESPVFEQAKRADD
RPQGPPLVEVLRRPGNLVLACAVGIGPFALTALISTYMISYATAIGYHRTDVMTALIFTSTTALITIPLFSALSDRVGRR
AVIVTGAIGIVCYAWPFYALVDMGSVPLLTVAMVIAQVLQSLMYAPLGALFSEMFGTRVRYTGASMGYQFAALLGAGFTP
LIASSLLAEGVSRTPLVVLAAGCGVVTIVAIWRVSETRGADLTDAGRLPRGAAAADR

Sequences:

>Translated_457_residues
MGFRPTGAPGNAKIGDMDETATPDPALLRRVAISSLLGTAIEYYDFLVYGTMSALVFGIIFFPESDPAVATIAAFGTLAA
GYVARPVGGILFGHFGDRIGRKSMLVLTMTLMGVASFLVGVLPTFAAIGVAAPILLVALRVIQGVAIGGEWGGATLMVTE
HAEPHRRGAWNGVMQMGSPIGSLLSVAVVTAITLMPDESFMSWGWRVPFLVSLLLLGLGIYVRLSITESPVFEQAKRADD
RPQGPPLVEVLRRPGNLVLACAVGIGPFALTALISTYMISYATAIGYHRTDVMTALIFTSTTALITIPLFSALSDRVGRR
AVIVTGAIGIVCYAWPFYALVDMGSVPLLTVAMVIAQVLQSLMYAPLGALFSEMFGTRVRYTGASMGYQFAALLGAGFTP
LIASSLLAEGVSRTPLVVLAAGCGVVTIVAIWRVSETRGADLTDAGRLPRGAAAADR
>Mature_456_residues
GFRPTGAPGNAKIGDMDETATPDPALLRRVAISSLLGTAIEYYDFLVYGTMSALVFGIIFFPESDPAVATIAAFGTLAAG
YVARPVGGILFGHFGDRIGRKSMLVLTMTLMGVASFLVGVLPTFAAIGVAAPILLVALRVIQGVAIGGEWGGATLMVTEH
AEPHRRGAWNGVMQMGSPIGSLLSVAVVTAITLMPDESFMSWGWRVPFLVSLLLLGLGIYVRLSITESPVFEQAKRADDR
PQGPPLVEVLRRPGNLVLACAVGIGPFALTALISTYMISYATAIGYHRTDVMTALIFTSTTALITIPLFSALSDRVGRRA
VIVTGAIGIVCYAWPFYALVDMGSVPLLTVAMVIAQVLQSLMYAPLGALFSEMFGTRVRYTGASMGYQFAALLGAGFTPL
IASSLLAEGVSRTPLVVLAAGCGVVTIVAIWRVSETRGADLTDAGRLPRGAAAADR

Specific function: Unknown

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=430, Percent_Identity=38.8372093023256, Blast_Score=302, Evalue=2e-83,
Organism=Escherichia coli, GI1789941, Length=438, Percent_Identity=38.3561643835616, Blast_Score=245, Evalue=4e-66,
Organism=Escherichia coli, GI1790550, Length=397, Percent_Identity=30.9823677581864, Blast_Score=208, Evalue=5e-55,
Organism=Escherichia coli, GI1788942, Length=408, Percent_Identity=28.6764705882353, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI87082404, Length=375, Percent_Identity=22.6666666666667, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87082231, Length=497, Percent_Identity=21.5291750503018, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47996; Mature: 47865

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFRPTGAPGNAKIGDMDETATPDPALLRRVAISSLLGTAIEYYDFLVYGTMSALVFGII
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FFPESDPAVATIAAFGTLAAGYVARPVGGILFGHFGDRIGRKSMLVLTMTLMGVASFLVG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
VLPTFAAIGVAAPILLVALRVIQGVAIGGEWGGATLMVTEHAEPHRRGAWNGVMQMGSPI
HHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCCEEEEEECCCCCHHCCHHHHHHHHCCHH
GSLLSVAVVTAITLMPDESFMSWGWRVPFLVSLLLLGLGIYVRLSITESPVFEQAKRADD
HHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHCCCC
RPQGPPLVEVLRRPGNLVLACAVGIGPFALTALISTYMISYATAIGYHRTDVMTALIFTS
CCCCCHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TTALITIPLFSALSDRVGRRAVIVTGAIGIVCYAWPFYALVDMGSVPLLTVAMVIAQVLQ
HHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
SLMYAPLGALFSEMFGTRVRYTGASMGYQFAALLGAGFTPLIASSLLAEGVSRTPLVVLA
HHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEE
AGCGVVTIVAIWRVSETRGADLTDAGRLPRGAAAADR
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GFRPTGAPGNAKIGDMDETATPDPALLRRVAISSLLGTAIEYYDFLVYGTMSALVFGII
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FFPESDPAVATIAAFGTLAAGYVARPVGGILFGHFGDRIGRKSMLVLTMTLMGVASFLVG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
VLPTFAAIGVAAPILLVALRVIQGVAIGGEWGGATLMVTEHAEPHRRGAWNGVMQMGSPI
HHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCCEEEEEECCCCCHHCCHHHHHHHHCCHH
GSLLSVAVVTAITLMPDESFMSWGWRVPFLVSLLLLGLGIYVRLSITESPVFEQAKRADD
HHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHCCCC
RPQGPPLVEVLRRPGNLVLACAVGIGPFALTALISTYMISYATAIGYHRTDVMTALIFTS
CCCCCHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TTALITIPLFSALSDRVGRRAVIVTGAIGIVCYAWPFYALVDMGSVPLLTVAMVIAQVLQ
HHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
SLMYAPLGALFSEMFGTRVRYTGASMGYQFAALLGAGFTPLIASSLLAEGVSRTPLVVLA
HHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEE
AGCGVVTIVAIWRVSETRGADLTDAGRLPRGAAAADR
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]