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The map label for this gene is 108797183

Identifier: 108797183

GI number: 108797183

Start: 224489

End: 225907

Strand: Direct

Name: 108797183

Synonym: Mmcs_0203

Alternate gene names: NA

Gene position: 224489-225907 (Clockwise)

Preceding gene: 108797182

Following gene: 108797187

Centisome position: 3.93

GC content: 66.53

Gene sequence:

>1419_bases
GTGAAACGGCTCAGTGGGTGGGACGCGTTCTTGCTATACAGCGAGACACCCACGGTGCACATGCACACCCTCAAGCTGGC
GGTGATCCAGCTCGACGATCTGGGCGGTCGCAGCTTCGGCGTCGAGGAGTTCCGGCAGGTCATCCACAGCCGGCTCTACA
AACTCGACCCGTTCCGGTACGAGCTCGTCGACATCCCGTTCAAGTTCCACCACCCGATGTGGCGGGAGAACTGCGAGGTC
GACCTCGAATACCACATCCGCCCGTACCGCGTCGAAAGTCCAGGCGGCCGCAGGCAACTCGACGAAGCGGTGGGCCGCAT
CGCGAGCACCCCGTTGGACCGCAGCCGACCGCTGTGGGAGATGTACTTCATCGAGGGGCTGGCCGACGGCAGGATCGCCG
TGCTCGGCAAGATCCACCACGCCCTCGCCGACGGCATCGCCTCGGCCAACCTCATGGCGCGCGGGATGGATCTGCAATCC
GGTCCGCAGCGCGACCGCGACTCCTACGCCACCGATCCGCCTCCGGGCCGTGGCGAGCTGGTCCGCAGCGCGTTCCGCGA
CCACCTGCGCCAGATCGGCAAGCTGCCGGCCACCTGGCGCTACACCGCCGAGGGTCTGCAACGGGTCCGGCGCAGCAACC
GCAAACTGTCACCCGAGTTGACCCGGCCGTTCACCCCACCCCCGTCGTTCATGAACCACAAGGTCGACGGGGTGCGCAAA
TTCGCCACGGCGACACTGGCTCTCGCCGACATCAAGCTGACCGCCAAACACCTCGGCGTCACGCTCAACGATCTGGTGCT
GGCGCTGTCGGCCGGAGCGCTGCGGCAGCTCTCGCTGCGCTACGACGGCCACGCCGACCACCCGCTGCTGGCGTCGGTGC
CGGTCAGCTTCGACTTCAACCCAGACCGGATCTCGGGCAACTACTTCACCGGTGTGCTGGTGTGCATCCCGGTTCAGGTC
GAGGATCCACTGCAGCGGGTGCAGGAGTGCCACAAGGCCGCGGCGTCGGCCAAGGAGGGGCATCACCTGATCGGTCCCGA
ACTGGTCAGCCGGTGGTCGTCGTACTTCCCGCCGGCACCGGCCGAGGCGCTGTTCCGTTGGCTGGCCAACAAAGACGGTC
AGAACAAGGTGCTCAACCTGCCGATCTCCAATGTGCCTGGCCCACGGGAACGGGGCCGGGTCGGGGGCGCACTGGTGACC
GAGATCTATTCAGTCGGCCCGTTGACCACCGGCAGTGGACTGAACATCACCGTCTGGAGCTACGTCGACCAGCTGAACAT
CTCGGTGCTCTCCGACGGTGCGACGCTGGAGGACCCCCACGAGCTCACCGACGCCATGGTCGAGGCGTTCGTCGAGATCC
GCACGGCCGCAGGACTTTCCGACGAGTTGACGGTGGTCGAGACGGCGATGGCGCCGTAG

Upstream 100 bases:

>100_bases
CCACCGCAATCGGGTTGTTCCTCAACCAGATTCACAAGAACCGTACCCTGGTCGGCGCGCGAGAGGTTATATAGACGCCT
CGAGCAGGAGGAGGCGATAC

Downstream 100 bases:

>100_bases
CCGGCTAGGCGACGGCCCGGGCGCGGGTGGCGTTCACCCACGACAGGAACTGATCCACCGCGTTGGCGGTGTAGTGCGCC
CGGGGAGATCCGAAGAAGTC

Product: diacylglycerol O-acyltransferase

Products: NA

Alternate protein names: Putative triacylglycerol synthase Rv0221/MT0231 [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MKRLSGWDAFLLYSETPTVHMHTLKLAVIQLDDLGGRSFGVEEFRQVIHSRLYKLDPFRYELVDIPFKFHHPMWRENCEV
DLEYHIRPYRVESPGGRRQLDEAVGRIASTPLDRSRPLWEMYFIEGLADGRIAVLGKIHHALADGIASANLMARGMDLQS
GPQRDRDSYATDPPPGRGELVRSAFRDHLRQIGKLPATWRYTAEGLQRVRRSNRKLSPELTRPFTPPPSFMNHKVDGVRK
FATATLALADIKLTAKHLGVTLNDLVLALSAGALRQLSLRYDGHADHPLLASVPVSFDFNPDRISGNYFTGVLVCIPVQV
EDPLQRVQECHKAAASAKEGHHLIGPELVSRWSSYFPPAPAEALFRWLANKDGQNKVLNLPISNVPGPRERGRVGGALVT
EIYSVGPLTTGSGLNITVWSYVDQLNISVLSDGATLEDPHELTDAMVEAFVEIRTAAGLSDELTVVETAMAP

Sequences:

>Translated_472_residues
MKRLSGWDAFLLYSETPTVHMHTLKLAVIQLDDLGGRSFGVEEFRQVIHSRLYKLDPFRYELVDIPFKFHHPMWRENCEV
DLEYHIRPYRVESPGGRRQLDEAVGRIASTPLDRSRPLWEMYFIEGLADGRIAVLGKIHHALADGIASANLMARGMDLQS
GPQRDRDSYATDPPPGRGELVRSAFRDHLRQIGKLPATWRYTAEGLQRVRRSNRKLSPELTRPFTPPPSFMNHKVDGVRK
FATATLALADIKLTAKHLGVTLNDLVLALSAGALRQLSLRYDGHADHPLLASVPVSFDFNPDRISGNYFTGVLVCIPVQV
EDPLQRVQECHKAAASAKEGHHLIGPELVSRWSSYFPPAPAEALFRWLANKDGQNKVLNLPISNVPGPRERGRVGGALVT
EIYSVGPLTTGSGLNITVWSYVDQLNISVLSDGATLEDPHELTDAMVEAFVEIRTAAGLSDELTVVETAMAP
>Mature_472_residues
MKRLSGWDAFLLYSETPTVHMHTLKLAVIQLDDLGGRSFGVEEFRQVIHSRLYKLDPFRYELVDIPFKFHHPMWRENCEV
DLEYHIRPYRVESPGGRRQLDEAVGRIASTPLDRSRPLWEMYFIEGLADGRIAVLGKIHHALADGIASANLMARGMDLQS
GPQRDRDSYATDPPPGRGELVRSAFRDHLRQIGKLPATWRYTAEGLQRVRRSNRKLSPELTRPFTPPPSFMNHKVDGVRK
FATATLALADIKLTAKHLGVTLNDLVLALSAGALRQLSLRYDGHADHPLLASVPVSFDFNPDRISGNYFTGVLVCIPVQV
EDPLQRVQECHKAAASAKEGHHLIGPELVSRWSSYFPPAPAEALFRWLANKDGQNKVLNLPISNVPGPRERGRVGGALVT
EIYSVGPLTTGSGLNITVWSYVDQLNISVLSDGATLEDPHELTDAMVEAFVEIRTAAGLSDELTVVETAMAP

Specific function: Upon expression in E.coli functions weakly as a triacylglycerol synthase, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Has very weak wax synthase activity, incorporating palmityl alcohol into wax esters in the presence of palmit

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the long-chain O-acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014292
- InterPro:   IPR009721
- InterPro:   IPR004255 [H]

Pfam domain/function: PF06974 DUF1298; PF03007 UPF0089 [H]

EC number: =2.3.1.20 [H]

Molecular weight: Translated: 52495; Mature: 52495

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLSGWDAFLLYSETPTVHMHTLKLAVIQLDDLGGRSFGVEEFRQVIHSRLYKLDPFRY
CCCCCCCCEEEEEECCCCEEEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEE
ELVDIPFKFHHPMWRENCEVDLEYHIRPYRVESPGGRRQLDEAVGRIASTPLDRSRPLWE
EEEECCHHHCCCHHHCCCCEEEEEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHH
MYFIEGLADGRIAVLGKIHHALADGIASANLMARGMDLQSGPQRDRDSYATDPPPGRGEL
HHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHH
VRSAFRDHLRQIGKLPATWRYTAEGLQRVRRSNRKLSPELTRPFTPPPSFMNHKVDGVRK
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHCCCCCCCHHHHCCCHHHHHH
FATATLALADIKLTAKHLGVTLNDLVLALSAGALRQLSLRYDGHADHPLLASVPVSFDFN
HHHHHHHHHHHEEEHHHHCCCHHHHHHHHHHCHHHHEEEEECCCCCCCEEEECCEEECCC
PDRISGNYFTGVLVCIPVQVEDPLQRVQECHKAAASAKEGHHLIGPELVSRWSSYFPPAP
CCCCCCCEEEEEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCC
AEALFRWLANKDGQNKVLNLPISNVPGPRERGRVGGALVTEIYSVGPLTTGSGLNITVWS
HHHHHHHHHCCCCCCCEEECCCCCCCCCHHHCCCCHHHHHHHHHCCCCCCCCCCEEEEEE
YVDQLNISVLSDGATLEDPHELTDAMVEAFVEIRTAAGLSDELTVVETAMAP
HHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHCCCC
>Mature Secondary Structure
MKRLSGWDAFLLYSETPTVHMHTLKLAVIQLDDLGGRSFGVEEFRQVIHSRLYKLDPFRY
CCCCCCCCEEEEEECCCCEEEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEE
ELVDIPFKFHHPMWRENCEVDLEYHIRPYRVESPGGRRQLDEAVGRIASTPLDRSRPLWE
EEEECCHHHCCCHHHCCCCEEEEEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHH
MYFIEGLADGRIAVLGKIHHALADGIASANLMARGMDLQSGPQRDRDSYATDPPPGRGEL
HHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHH
VRSAFRDHLRQIGKLPATWRYTAEGLQRVRRSNRKLSPELTRPFTPPPSFMNHKVDGVRK
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHCCCCCCCHHHHCCCHHHHHH
FATATLALADIKLTAKHLGVTLNDLVLALSAGALRQLSLRYDGHADHPLLASVPVSFDFN
HHHHHHHHHHHEEEHHHHCCCHHHHHHHHHHCHHHHEEEEECCCCCCCEEEECCEEECCC
PDRISGNYFTGVLVCIPVQVEDPLQRVQECHKAAASAKEGHHLIGPELVSRWSSYFPPAP
CCCCCCCEEEEEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCC
AEALFRWLANKDGQNKVLNLPISNVPGPRERGRVGGALVTEIYSVGPLTTGSGLNITVWS
HHHHHHHHHCCCCCCCEEECCCCCCCCCHHHCCCCHHHHHHHHHCCCCCCCCCCEEEEEE
YVDQLNISVLSDGATLEDPHELTDAMVEAFVEIRTAAGLSDELTVVETAMAP
HHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]