Click here to switch to the map view.

The map label for this gene is miaE [H]

Identifier: 108763382

GI number: 108763382

Start: 1479950

End: 1480573

Strand: Reverse

Name: miaE [H]

Synonym: MXAN_1266

Alternate gene names: 108763382

Gene position: 1480573-1479950 (Counterclockwise)

Preceding gene: 108763113

Following gene: 108757754

Centisome position: 16.2

GC content: 71.47

Gene sequence:

>624_bases
ATGAGCCGTCCCACGCCTTCCCGCCGTCCCCTCTCTGGAGAGGGCCCCGTCATTCTTCACGCCGCCACCGACCCGCGCTG
GCTGCCCCTGGCGCTCGAGCGGTTCGACGAGGTGCTGGTGGACCATGCCCACTGCGAGAAGAAGGCCGCCGCCAACGCGC
TGTCCATGCTCCAGGCCTACCCGGACCTGCCCGGGCTGCCCTCGCAGATGGCGCGGCTGGCGCGCGAGGAGAGCGCTCAC
CTGGCCCGCGTATTGGACTTGATGGCGGCGCGCGGCCTCACGCTGACCAAGGACGCGGGAGACCCCTACGCCCAAGGCCT
GCAGAAGCTGATTCGCACGCCCGCCGCCGAGCGCCGCATGGACCGGCTGCTGGTGGCCGCCGTCATCGAAGCCCGCTCCT
GCGAGCGGCTGTCCCTGCTGGCCGAAGGCCTCACCGACCCGGCCCTGGCGCGCTTCTACGGCGAGCTGGCCCAGTCCGAG
GACGGCCATCAGTCCCTCTTCTACCGGCTGGCCGTCACCGCGTCCGAAGGCGACGAAACCAGCGTGAAGGCGCGGCTGGA
GTGGATGCTGGAGCGCGAGGCGCAGGTCATCACCGACGTGGGGCTGCGCGCCGCCATCCACTGA

Upstream 100 bases:

>100_bases
AGCACCGCCCGCATCGCCCGCAAGGTCGGCTACCTGGCCGGACGGACGTGGCGGCTCGTCCTGGTGGAGTAGCGGCCGGC
CGCGTAAAGTCCGCGCCCGC

Downstream 100 bases:

>100_bases
GCCGTCACGGCCCCGTGGGCGCCCGCAGGCCCACGGCCGGGCGATGCGCCACCGGCGCCGGCGAGGAGCCCTTCTCCAGC
GGCCACGCCCAGGGCACGGC

Product: tRNA-(MS[2]IO[6]A)-hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 207; Mature: 206

Protein sequence:

>207_residues
MSRPTPSRRPLSGEGPVILHAATDPRWLPLALERFDEVLVDHAHCEKKAAANALSMLQAYPDLPGLPSQMARLAREESAH
LARVLDLMAARGLTLTKDAGDPYAQGLQKLIRTPAAERRMDRLLVAAVIEARSCERLSLLAEGLTDPALARFYGELAQSE
DGHQSLFYRLAVTASEGDETSVKARLEWMLEREAQVITDVGLRAAIH

Sequences:

>Translated_207_residues
MSRPTPSRRPLSGEGPVILHAATDPRWLPLALERFDEVLVDHAHCEKKAAANALSMLQAYPDLPGLPSQMARLAREESAH
LARVLDLMAARGLTLTKDAGDPYAQGLQKLIRTPAAERRMDRLLVAAVIEARSCERLSLLAEGLTDPALARFYGELAQSE
DGHQSLFYRLAVTASEGDETSVKARLEWMLEREAQVITDVGLRAAIH
>Mature_206_residues
SRPTPSRRPLSGEGPVILHAATDPRWLPLALERFDEVLVDHAHCEKKAAANALSMLQAYPDLPGLPSQMARLAREESAHL
ARVLDLMAARGLTLTKDAGDPYAQGLQKLIRTPAAERRMDRLLVAAVIEARSCERLSLLAEGLTDPALARFYGELAQSED
GHQSLFYRLAVTASEGDETSVKARLEWMLEREAQVITDVGLRAAIH

Specific function: Production of the modified nucleoside 2-methylthio-cis- ribozeatin (MS[2]IO[6]A) found in some tRNAs. Catalyzes the oxygen-dependent transformation of MS[2]I[6]A into MS[2]IO[6]A [H]

COG id: COG4445

COG function: function code FJ; Hydroxylase for synthesis of 2-methylthio-cis-ribozeatin in tRNA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012347
- InterPro:   IPR010386 [H]

Pfam domain/function: PF06175 MiaE [H]

EC number: NA

Molecular weight: Translated: 22740; Mature: 22609

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRPTPSRRPLSGEGPVILHAATDPRWLPLALERFDEVLVDHAHCEKKAAANALSMLQAY
CCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PDLPGLPSQMARLAREESAHLARVLDLMAARGLTLTKDAGDPYAQGLQKLIRTPAAERRM
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHCCHHHHHH
DRLLVAAVIEARSCERLSLLAEGLTDPALARFYGELAQSEDGHQSLFYRLAVTASEGDET
HHHHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHEEECCCCCHH
SVKARLEWMLEREAQVITDVGLRAAIH
HHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SRPTPSRRPLSGEGPVILHAATDPRWLPLALERFDEVLVDHAHCEKKAAANALSMLQAY
CCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PDLPGLPSQMARLAREESAHLARVLDLMAARGLTLTKDAGDPYAQGLQKLIRTPAAERRM
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHCCHHHHHH
DRLLVAAVIEARSCERLSLLAEGLTDPALARFYGELAQSEDGHQSLFYRLAVTASEGDET
HHHHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHEEECCCCCHH
SVKARLEWMLEREAQVITDVGLRAAIH
HHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253666; 11677609 [H]