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The map label for this gene is ykfB [H]

Identifier: 108761657

GI number: 108761657

Start: 4112005

End: 4113165

Strand: Direct

Name: ykfB [H]

Synonym: MXAN_3531

Alternate gene names: 108761657

Gene position: 4112005-4113165 (Clockwise)

Preceding gene: 108760974

Following gene: 108763899

Centisome position: 44.99

GC content: 71.92

Gene sequence:

>1161_bases
ATGCGGATTGCCCAGGTTTCGCTCATTCCCCTCCGGTTGGAGCTGCGCCAGCCCCTGAAGACGTCACAAGGGGGGCACGC
GGTGCGAGAAGGCTTCCTCGTCCGCGTCGTCGACGAGGAGGGGCGCGAAGGTCTGGGCGAGGCGATGCCGCTGCCCGCGT
TCGGCACCGAGTCGCTCGAAGACTGTGGCGTGACGCTGAACGCCTGGCTGTCCGAGCTGAAGGGCCAGTTCCTGGGCGAC
TCGGTCCGGGCCATCGAGGACACGTTGTCGCCGTTCCCCCCTGCCGTGGCGCGAGGCGACGGCGTGCGCATCCGTGCACG
TCAGGTGACGCCAGCGGGCGCCATGCCGGCCGCGGAACACGCGCTGGAGCTGGCGCTGCTGGACCTGCTCGCGCAACGCC
AGGGTGTTCCGCTGTGCTGGCTGCTGGCGGAGGAGGCGCGGACGGAAGTGGCGGTGAACGCGCTGCTGGGCGCCGACACC
CCCGAGGCCCTGGCGGGAGAGGCCCGGCAGGCCGTGGCCGAGGGCTTCGGCACGTTGAAGCTGAAAGTCGCCGGCCGGCC
GCTGGACGCCGACGAGGCGCGAGTGAAGGCCGTGCGCGATGCGGTGGGGCCGGACGTGAAGCTTCGGCTGGATGCGAACG
GCGGCTGGTCCGAGCCCGACGCGAAGCGCGCCCTGGACCGGCTGGGTTGGTACGGCCTGGAGTTGGTGGAGCAGCCCACG
CCGCCGGAGGACCTCGCGGCGCTCTGGCGCGTTCAGCGCCGCGCCCCCTGCACGGTGGCGGCGGACGAGTCCCTGGCTTC
TCCCGATGCGCTCCGGGCGCTGCTGACGGTGGACCCGTTGCTGGGCGGTGGCCCGGCGGTGGGCGCGGTGGTGCTGAAGC
CCATGGTGCTGGGCGGACTGTTGCCGTGCCTCGTGGTCGCGATGCGCGCGGCGCGGCTCGGAATGCAGGCCTATGTCACC
AGCTCCATTGATGGCGTGGTGGCGCGGGCTGGCGCGGCACACCTCGCGGCGGCACTGCCATCCGGAGCGCTGGCCTCCGG
GCTCGCTATTGGGCGCTTGCTGGCGAAGGAGCCGGAAGATCATCCGTACCATCCGCAGCAAGGCGTCATTCGCCTGTCGA
GCACCCCGGGGCTCGGACTGAGCTCCCAGTCCCTGGTGTGA

Upstream 100 bases:

>100_bases
GGGGGCCGCGCTGAATCCGGCGCTGGGGTCCACGGCCAGACTCCAGCTCGTGTTTGGAGTGCTGTTCGCGTTGGGCCTCT
ACCTGCGATGATGTGAGGCC

Downstream 100 bases:

>100_bases
CGGCTCGGCGCATGGGAGCCCCCGGCTTGGCCTCCGTTGATGAAGCCCAAGGTGACGGAATGTCGGTCCTGCTGTGTCCC
ATTCGAGTAGGCGCTCGTCA

Product: putative O-succinylbenzoic acid synthase

Products: NA

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MRIAQVSLIPLRLELRQPLKTSQGGHAVREGFLVRVVDEEGREGLGEAMPLPAFGTESLEDCGVTLNAWLSELKGQFLGD
SVRAIEDTLSPFPPAVARGDGVRIRARQVTPAGAMPAAEHALELALLDLLAQRQGVPLCWLLAEEARTEVAVNALLGADT
PEALAGEARQAVAEGFGTLKLKVAGRPLDADEARVKAVRDAVGPDVKLRLDANGGWSEPDAKRALDRLGWYGLELVEQPT
PPEDLAALWRVQRRAPCTVAADESLASPDALRALLTVDPLLGGGPAVGAVVLKPMVLGGLLPCLVVAMRAARLGMQAYVT
SSIDGVVARAGAAHLAAALPSGALASGLAIGRLLAKEPEDHPYHPQQGVIRLSSTPGLGLSSQSLV

Sequences:

>Translated_386_residues
MRIAQVSLIPLRLELRQPLKTSQGGHAVREGFLVRVVDEEGREGLGEAMPLPAFGTESLEDCGVTLNAWLSELKGQFLGD
SVRAIEDTLSPFPPAVARGDGVRIRARQVTPAGAMPAAEHALELALLDLLAQRQGVPLCWLLAEEARTEVAVNALLGADT
PEALAGEARQAVAEGFGTLKLKVAGRPLDADEARVKAVRDAVGPDVKLRLDANGGWSEPDAKRALDRLGWYGLELVEQPT
PPEDLAALWRVQRRAPCTVAADESLASPDALRALLTVDPLLGGGPAVGAVVLKPMVLGGLLPCLVVAMRAARLGMQAYVT
SSIDGVVARAGAAHLAAALPSGALASGLAIGRLLAKEPEDHPYHPQQGVIRLSSTPGLGLSSQSLV
>Mature_386_residues
MRIAQVSLIPLRLELRQPLKTSQGGHAVREGFLVRVVDEEGREGLGEAMPLPAFGTESLEDCGVTLNAWLSELKGQFLGD
SVRAIEDTLSPFPPAVARGDGVRIRARQVTPAGAMPAAEHALELALLDLLAQRQGVPLCWLLAEEARTEVAVNALLGADT
PEALAGEARQAVAEGFGTLKLKVAGRPLDADEARVKAVRDAVGPDVKLRLDANGGWSEPDAKRALDRLGWYGLELVEQPT
PPEDLAALWRVQRRAPCTVAADESLASPDALRALLTVDPLLGGGPAVGAVVLKPMVLGGLLPCLVVAMRAARLGMQAYVT
SSIDGVVARAGAAHLAAALPSGALASGLAIGRLLAKEPEDHPYHPQQGVIRLSSTPGLGLSSQSLV

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has probably a role in the metabolism of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of the other Ala-X dipepti

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 40397; Mature: 40397

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIAQVSLIPLRLELRQPLKTSQGGHAVREGFLVRVVDEEGREGLGEAMPLPAFGTESLE
CCEEEEEEEEEHHHHHCCCCCCCCCHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCHH
DCGVTLNAWLSELKGQFLGDSVRAIEDTLSPFPPAVARGDGVRIRARQVTPAGAMPAAEH
HCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCHHHH
ALELALLDLLAQRQGVPLCWLLAEEARTEVAVNALLGADTPEALAGEARQAVAEGFGTLK
HHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEE
LKVAGRPLDADEARVKAVRDAVGPDVKLRLDANGGWSEPDAKRALDRLGWYGLELVEQPT
EEECCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCHHHHCCCC
PPEDLAALWRVQRRAPCTVAADESLASPDALRALLTVDPLLGGGPAVGAVVLKPMVLGGL
CHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
LPCLVVAMRAARLGMQAYVTSSIDGVVARAGAAHLAAALPSGALASGLAIGRLLAKEPED
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCC
HPYHPQQGVIRLSSTPGLGLSSQSLV
CCCCCCCCEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MRIAQVSLIPLRLELRQPLKTSQGGHAVREGFLVRVVDEEGREGLGEAMPLPAFGTESLE
CCEEEEEEEEEHHHHHCCCCCCCCCHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCHH
DCGVTLNAWLSELKGQFLGDSVRAIEDTLSPFPPAVARGDGVRIRARQVTPAGAMPAAEH
HCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCHHHH
ALELALLDLLAQRQGVPLCWLLAEEARTEVAVNALLGADTPEALAGEARQAVAEGFGTLK
HHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEE
LKVAGRPLDADEARVKAVRDAVGPDVKLRLDANGGWSEPDAKRALDRLGWYGLELVEQPT
EEECCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCHHHHCCCC
PPEDLAALWRVQRRAPCTVAADESLASPDALRALLTVDPLLGGGPAVGAVVLKPMVLGGL
CHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
LPCLVVAMRAARLGMQAYVTSSIDGVVARAGAAHLAAALPSGALASGLAIGRLLAKEPED
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCC
HPYHPQQGVIRLSSTPGLGLSSQSLV
CCCCCCCCEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]