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The map label for this gene is fadD26 [H]

Identifier: 108761377

GI number: 108761377

Start: 5438932

End: 5440677

Strand: Direct

Name: fadD26 [H]

Synonym: MXAN_4405

Alternate gene names: 108761377

Gene position: 5438932-5440677 (Clockwise)

Preceding gene: 108763601

Following gene: 108762755

Centisome position: 59.51

GC content: 67.98

Gene sequence:

>1746_bases
ATGCCGAGTCCCAGCCGTTCCGTACAGACCCTGACCGACCTGCTGCGCTGGCGCGCCTCGAACCAGACCGAGGCCCGCGC
ATATACGTTCCTCCAGGATGGCGAAGGCCAGGAGACCACCTGGACCTATGGCGATTTGGATCGCCAGGCCCGCGCCATTG
CCGCGGCGCTCCAGGAGCACAAGGGCGCTGGCGAGCGAGCGCTGCTGCTGTATCCACCGGGGCTCGACTACATCGCGGCG
TTCTTCGGCAGCCTGTACGCGGGCGTGGCCGCGGTGCCAGCCTATCCTCCCGCGCAGCTCCAGGCCGTCACGCGCATCCT
CTCCATCCTCCAGGATGCGAAGCCACGCTTCGCGCTCACCACGCACGAAATCCTGGAGAGCGTGAACGCACTGGCCGAGG
CCTACCCCGTCCTGAAGGACATCCGCTGGATTGCGACGGACGCGCTGGAGGATGGGCTGGAGGATGGCTGGAAGCGCCCC
GCCATCACCGGCGACACGCTGGCCTTCCTCCAGTACACGTCAGGCTCCACGTCGACGCCCAAGGGCGTGATGGTGCTGCA
CCGGAACCTGATGTCCAACGAGGAGATGATCCGGCAGGGCTTCAGCCACGACGACCCGCCGACGATATGCGGCTGGCTGC
CGCTGTACCACGACATGGGACTCATCGGCACGGTGCTCCAGCCGATGTACCTGGGATCGCACTCCATCGTGATGTCCCCG
TGGTCCTTCCTGCAGCGCCCCATCCGCTGGCTGAACACCATCACGAAGTACCGTGCGACGACGAGCGGCGGGCCCAACTT
CGCCTACGCGCTCTGCACGCGAAAGGTGAAGCCGGAGAAGCTCGCTTCGCTAGACCTGAGTTCGTGGCGCGTCGCCTTCA
ATGGCGCGGAGCCCGTGCGGGCGGAGACGCTGGTGGAGTTCGCGGACACCTTCGCGCCCGCCGGCTTCCGCCGGGAGGCC
TTCTATCCCTGCTATGGCCTCGCGGAGGCGACGCTCTTCGTGTCGGGAGGCATCACCTCCGAGTTGCCACGCCACCTCAC
CGTGGATTCGGCGGCGCTCGAGCAGAACCGCGCCGTGGCCGCCCAGCCGGGCCCGTCCGCGCGAACGCTCGTCAGCTCCG
GGCGGAGCTGGGGTGACAGCCTCATCCGCATCGTGAATCCAGAGACGCTGGTGGCGTGCGCACCGGGCGAGGTGGGCGAA
ATCTGGACGGCCGGTCCCCACGTCACTCACGGCTACTGGGGACGCGAGGAGACGAACGCAGAGACCTTCCAGGCGCGCAT
CCAGGGCAGCGAGGAAGGTCCCTTCCTGCGTACCGGCGATTTGGGCTTCATGATTGACGGCGATCTGTATGTCACCGGCC
GCATCAAGGACCTCATCATCGTCGACGGTCGGAACCACTACCCGCAGGACCTCGAGCTGACGGCCGAGAGCCAGCACCCG
GCGCTGCGCCCTGGCTGCTCGGTGGCGTTCTCCGTCGACCACCCGGAGGGCGAGCGGCTGGTGGTGCTGGTGGAGGTCAG
CGCCCGCTTCGCTCCATCCGAACAGGGTGATGGCGCCCAGTCCCTGAACGGGAGCGCGCTCCAGAAGACCATCCGGCAGG
CCGTGGCCGCCGCGCACTCCGTGGACGTCCATGAGGTCGTCCTGCTCCAGCAGGGCGAGGTCCTCAAGACGTCCAGCGGC
AAGGTCCAGCGGCGCGCCTGCCGGGCGAAGTACGTGGAGGGCAGCCTCCAGCGCTGGGCCGCGTAG

Upstream 100 bases:

>100_bases
GCGTGAGCTGGACGTGCCCCCTCAGGACGCGCCTTCCGCCGAACCCGCGGCGAGCCCCTGAACCCTCCCCTCTTCCCACG
ACTTCCCAAGGAGACCCGCC

Downstream 100 bases:

>100_bases
TCCGTCCCGCGCGGCATGTTGAAGCAGGCCCTCGCTACCTGCGAGGTCCGAGCACGATGATCGCCATCCCCAGGATGGCG
ACGAGCGCGCCCACGAGGTC

Product: peptide synthetase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 581; Mature: 580

Protein sequence:

>581_residues
MPSPSRSVQTLTDLLRWRASNQTEARAYTFLQDGEGQETTWTYGDLDRQARAIAAALQEHKGAGERALLLYPPGLDYIAA
FFGSLYAGVAAVPAYPPAQLQAVTRILSILQDAKPRFALTTHEILESVNALAEAYPVLKDIRWIATDALEDGLEDGWKRP
AITGDTLAFLQYTSGSTSTPKGVMVLHRNLMSNEEMIRQGFSHDDPPTICGWLPLYHDMGLIGTVLQPMYLGSHSIVMSP
WSFLQRPIRWLNTITKYRATTSGGPNFAYALCTRKVKPEKLASLDLSSWRVAFNGAEPVRAETLVEFADTFAPAGFRREA
FYPCYGLAEATLFVSGGITSELPRHLTVDSAALEQNRAVAAQPGPSARTLVSSGRSWGDSLIRIVNPETLVACAPGEVGE
IWTAGPHVTHGYWGREETNAETFQARIQGSEEGPFLRTGDLGFMIDGDLYVTGRIKDLIIVDGRNHYPQDLELTAESQHP
ALRPGCSVAFSVDHPEGERLVVLVEVSARFAPSEQGDGAQSLNGSALQKTIRQAVAAAHSVDVHEVVLLQQGEVLKTSSG
KVQRRACRAKYVEGSLQRWAA

Sequences:

>Translated_581_residues
MPSPSRSVQTLTDLLRWRASNQTEARAYTFLQDGEGQETTWTYGDLDRQARAIAAALQEHKGAGERALLLYPPGLDYIAA
FFGSLYAGVAAVPAYPPAQLQAVTRILSILQDAKPRFALTTHEILESVNALAEAYPVLKDIRWIATDALEDGLEDGWKRP
AITGDTLAFLQYTSGSTSTPKGVMVLHRNLMSNEEMIRQGFSHDDPPTICGWLPLYHDMGLIGTVLQPMYLGSHSIVMSP
WSFLQRPIRWLNTITKYRATTSGGPNFAYALCTRKVKPEKLASLDLSSWRVAFNGAEPVRAETLVEFADTFAPAGFRREA
FYPCYGLAEATLFVSGGITSELPRHLTVDSAALEQNRAVAAQPGPSARTLVSSGRSWGDSLIRIVNPETLVACAPGEVGE
IWTAGPHVTHGYWGREETNAETFQARIQGSEEGPFLRTGDLGFMIDGDLYVTGRIKDLIIVDGRNHYPQDLELTAESQHP
ALRPGCSVAFSVDHPEGERLVVLVEVSARFAPSEQGDGAQSLNGSALQKTIRQAVAAAHSVDVHEVVLLQQGEVLKTSSG
KVQRRACRAKYVEGSLQRWAA
>Mature_580_residues
PSPSRSVQTLTDLLRWRASNQTEARAYTFLQDGEGQETTWTYGDLDRQARAIAAALQEHKGAGERALLLYPPGLDYIAAF
FGSLYAGVAAVPAYPPAQLQAVTRILSILQDAKPRFALTTHEILESVNALAEAYPVLKDIRWIATDALEDGLEDGWKRPA
ITGDTLAFLQYTSGSTSTPKGVMVLHRNLMSNEEMIRQGFSHDDPPTICGWLPLYHDMGLIGTVLQPMYLGSHSIVMSPW
SFLQRPIRWLNTITKYRATTSGGPNFAYALCTRKVKPEKLASLDLSSWRVAFNGAEPVRAETLVEFADTFAPAGFRREAF
YPCYGLAEATLFVSGGITSELPRHLTVDSAALEQNRAVAAQPGPSARTLVSSGRSWGDSLIRIVNPETLVACAPGEVGEI
WTAGPHVTHGYWGREETNAETFQARIQGSEEGPFLRTGDLGFMIDGDLYVTGRIKDLIIVDGRNHYPQDLELTAESQHPA
LRPGCSVAFSVDHPEGERLVVLVEVSARFAPSEQGDGAQSLNGSALQKTIRQAVAAAHSVDVHEVVLLQQGEVLKTSSGK
VQRRACRAKYVEGSLQRWAA

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI44888818, Length=513, Percent_Identity=26.9005847953216, Blast_Score=144, Evalue=3e-34,
Organism=Homo sapiens, GI55749758, Length=504, Percent_Identity=26.3888888888889, Blast_Score=139, Evalue=7e-33,
Organism=Homo sapiens, GI225735629, Length=506, Percent_Identity=26.2845849802372, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI45827692, Length=506, Percent_Identity=26.2845849802372, Blast_Score=135, Evalue=2e-31,
Organism=Homo sapiens, GI225735625, Length=609, Percent_Identity=22.9885057471264, Blast_Score=96, Evalue=9e-20,
Organism=Homo sapiens, GI45827694, Length=537, Percent_Identity=23.6499068901304, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI225735627, Length=510, Percent_Identity=23.5294117647059, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI45827696, Length=510, Percent_Identity=23.5294117647059, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI45827698, Length=473, Percent_Identity=23.4672304439746, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1788107, Length=533, Percent_Identity=25.891181988743, Blast_Score=123, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI193202679, Length=617, Percent_Identity=23.8249594813614, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17531443, Length=445, Percent_Identity=26.5168539325843, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24654656, Length=455, Percent_Identity=26.3736263736264, Blast_Score=150, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 63491; Mature: 63360

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSPSRSVQTLTDLLRWRASNQTEARAYTFLQDGEGQETTWTYGDLDRQARAIAAALQEH
CCCCCHHHHHHHHHHHHCCCCCCCHHEEEEEECCCCCCCEEECCCHHHHHHHHHHHHHHH
KGAGERALLLYPPGLDYIAAFFGSLYAGVAAVPAYPPAQLQAVTRILSILQDAKPRFALT
CCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEE
THEILESVNALAEAYPVLKDIRWIATDALEDGLEDGWKRPAITGDTLAFLQYTSGSTSTP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCC
KGVMVLHRNLMSNEEMIRQGFSHDDPPTICGWLPLYHDMGLIGTVLQPMYLGSHSIVMSP
CHHHHHHHHHHCCHHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCEEECC
WSFLQRPIRWLNTITKYRATTSGGPNFAYALCTRKVKPEKLASLDLSSWRVAFNGAEPVR
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHCCCCHHHHHHCCCCCEEEEECCCCCHH
AETLVEFADTFAPAGFRREAFYPCYGLAEATLFVSGGITSELPRHLTVDSAALEQNRAVA
HHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCHHHCCCCEEECHHHHHCCCEEE
AQPGPSARTLVSSGRSWGDSLIRIVNPETLVACAPGEVGEIWTAGPHVTHGYWGREETNA
CCCCCCHHHHHHCCCCCCCCEEEEECCCEEEEECCCCCCCEECCCCCCCCCCCCCCCCCH
ETFQARIQGSEEGPFLRTGDLGFMIDGDLYVTGRIKDLIIVDGRNHYPQDLELTAESQHP
HHHHHEECCCCCCCEEEECCEEEEECCCEEEEECEEEEEEECCCCCCCCCCEEEECCCCC
ALRPGCSVAFSVDHPEGERLVVLVEVSARFAPSEQGDGAQSLNGSALQKTIRQAVAAAHS
CCCCCCCEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
VDVHEVVLLQQGEVLKTSSGKVQRRACRAKYVEGSLQRWAA
CCHHHEEEECCCCEEECCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PSPSRSVQTLTDLLRWRASNQTEARAYTFLQDGEGQETTWTYGDLDRQARAIAAALQEH
CCCCHHHHHHHHHHHHCCCCCCCHHEEEEEECCCCCCCEEECCCHHHHHHHHHHHHHHH
KGAGERALLLYPPGLDYIAAFFGSLYAGVAAVPAYPPAQLQAVTRILSILQDAKPRFALT
CCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEE
THEILESVNALAEAYPVLKDIRWIATDALEDGLEDGWKRPAITGDTLAFLQYTSGSTSTP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCC
KGVMVLHRNLMSNEEMIRQGFSHDDPPTICGWLPLYHDMGLIGTVLQPMYLGSHSIVMSP
CHHHHHHHHHHCCHHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCEEECC
WSFLQRPIRWLNTITKYRATTSGGPNFAYALCTRKVKPEKLASLDLSSWRVAFNGAEPVR
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHCCCCHHHHHHCCCCCEEEEECCCCCHH
AETLVEFADTFAPAGFRREAFYPCYGLAEATLFVSGGITSELPRHLTVDSAALEQNRAVA
HHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCHHHCCCCEEECHHHHHCCCEEE
AQPGPSARTLVSSGRSWGDSLIRIVNPETLVACAPGEVGEIWTAGPHVTHGYWGREETNA
CCCCCCHHHHHHCCCCCCCCEEEEECCCEEEEECCCCCCCEECCCCCCCCCCCCCCCCCH
ETFQARIQGSEEGPFLRTGDLGFMIDGDLYVTGRIKDLIIVDGRNHYPQDLELTAESQHP
HHHHHEECCCCCCCEEEECCEEEEECCCEEEEECEEEEEEECCCCCCCCCCEEEECCCCC
ALRPGCSVAFSVDHPEGERLVVLVEVSARFAPSEQGDGAQSLNGSALQKTIRQAVAAAHS
CCCCCCCEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
VDVHEVVLLQQGEVLKTSSGKVQRRACRAKYVEGSLQRWAA
CCHHHEEEECCCCEEECCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]