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The map label for this gene is ddl [H]

Identifier: 108760939

GI number: 108760939

Start: 6963169

End: 6964125

Strand: Reverse

Name: ddl [H]

Synonym: MXAN_5601

Alternate gene names: 108760939

Gene position: 6964125-6963169 (Counterclockwise)

Preceding gene: 108762211

Following gene: 108761470

Centisome position: 76.2

GC content: 68.13

Gene sequence:

>957_bases
GTGACTCCGAACCGCGGTGCCTTCACGAAGGACGAGCTCAAGCAGAAGCGTGTCGGTGTACTGTTGGGCGGAATGTCCGC
GGAGCGCGACGTGTCCCTGCGCACCGGTGAGGCCGTCTCCGGGGCGCTGCGCGGGCTGGGCTACGACGTGGTGGAGATTG
ACGTGGGCCGGGATTTGCCCGCGCGTCTGGCGGCGGAGAAGGTGGACGTGGCGTGGCTGGCCGTCCACGGGCGTTATGGC
GAGGACGGCTGTCTCCAGGGACTGCTGGAGTCGCTCTTCATTCCTTATACCGGCAGCGGCGTGCTGGCCTCCGCGCTGGG
CATGGACAAGGTGTACGCCAAGCAGGTCTACGTGGCCCACGGCATCCCCACGCCCGCGTACCGTTCCTTTAAGGACGCCG
CGTCGGCGCTGGCGGCGGCGGACAGCCTGCCCTTCCCGTTTCCAGTGGTGGTGAAGCCCAGCCGCGAAGGCAGCAGCGTG
GGCGTGCACATCTGCAAGACGCGGGACGCCTACGAGGCCGCGGTGACGGACGCGGCGAAGTACGCGGGCACCCTGCTGGT
GGAGCAGTTCGTCAAGGGACGCGAAGTGCAGGGTGGCGTGCTGGACGATGAGGCCCTGGGCGTCATCGAGGTACGCGCCG
CGCGCGAGTTCTACGACTATGACGCGAAGTACAAGGCGGGCACCGGCACGCAGTACCTCTTCCCCGCGCCCCTTCCTCCG
GATCAGTATGCGCGGGTGAACGAGGTGTGCCTGGCCGCGCACCAGGCCCTGGGGTGTAGCGGGGGCTCGCGGTCGGACGT
CATCGTCACCGACGGAGGCGATGTGTTCCTGCTGGAAACCAACACGCTGCCGGGCATGACGGCCTCGAGCCTCTTGCCCA
AGATTGCCGCGGGGCGCGGTATCGACTTTCCCGCCCTGTGTGAGCGCCTGCTGCTGGGCGCGTGTCTCAAGGCCTGA

Upstream 100 bases:

>100_bases
CCCGCGACGTGCTGGGACTCGTCACCCTCATGCAGCAGCGGGTGCTCGAGCAGTCCGGCGTTGACATGAAACCCGAAGTC
AAGCGCCTGGGAGACTTCCT

Downstream 100 bases:

>100_bases
GCATCTGGTATGGGGCCGGCGCAACTTGGCGTCGGTCTCCTTCGAAAATTTCCGAAGCGCCTGTGTGGCGGCTTGCGCTA
CAGCGACGTGCCTGTAGCCA

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MTPNRGAFTKDELKQKRVGVLLGGMSAERDVSLRTGEAVSGALRGLGYDVVEIDVGRDLPARLAAEKVDVAWLAVHGRYG
EDGCLQGLLESLFIPYTGSGVLASALGMDKVYAKQVYVAHGIPTPAYRSFKDAASALAAADSLPFPFPVVVKPSREGSSV
GVHICKTRDAYEAAVTDAAKYAGTLLVEQFVKGREVQGGVLDDEALGVIEVRAAREFYDYDAKYKAGTGTQYLFPAPLPP
DQYARVNEVCLAAHQALGCSGGSRSDVIVTDGGDVFLLETNTLPGMTASSLLPKIAAGRGIDFPALCERLLLGACLKA

Sequences:

>Translated_318_residues
MTPNRGAFTKDELKQKRVGVLLGGMSAERDVSLRTGEAVSGALRGLGYDVVEIDVGRDLPARLAAEKVDVAWLAVHGRYG
EDGCLQGLLESLFIPYTGSGVLASALGMDKVYAKQVYVAHGIPTPAYRSFKDAASALAAADSLPFPFPVVVKPSREGSSV
GVHICKTRDAYEAAVTDAAKYAGTLLVEQFVKGREVQGGVLDDEALGVIEVRAAREFYDYDAKYKAGTGTQYLFPAPLPP
DQYARVNEVCLAAHQALGCSGGSRSDVIVTDGGDVFLLETNTLPGMTASSLLPKIAAGRGIDFPALCERLLLGACLKA
>Mature_317_residues
TPNRGAFTKDELKQKRVGVLLGGMSAERDVSLRTGEAVSGALRGLGYDVVEIDVGRDLPARLAAEKVDVAWLAVHGRYGE
DGCLQGLLESLFIPYTGSGVLASALGMDKVYAKQVYVAHGIPTPAYRSFKDAASALAAADSLPFPFPVVVKPSREGSSVG
VHICKTRDAYEAAVTDAAKYAGTLLVEQFVKGREVQGGVLDDEALGVIEVRAAREFYDYDAKYKAGTGTQYLFPAPLPPD
QYARVNEVCLAAHQALGCSGGSRSDVIVTDGGDVFLLETNTLPGMTASSLLPKIAAGRGIDFPALCERLLLGACLKA

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786280, Length=300, Percent_Identity=40.3333333333333, Blast_Score=205, Evalue=3e-54,
Organism=Escherichia coli, GI1786579, Length=348, Percent_Identity=31.3218390804598, Blast_Score=160, Evalue=7e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 33574; Mature: 33442

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPNRGAFTKDELKQKRVGVLLGGMSAERDVSLRTGEAVSGALRGLGYDVVEIDVGRDLP
CCCCCCCCCHHHHHHHHHCEEEECCCCCCCCEEECCHHHHHHHHHCCCEEEEEECCCCCH
ARLAAEKVDVAWLAVHGRYGEDGCLQGLLESLFIPYTGSGVLASALGMDKVYAKQVYVAH
HHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHCHHHHHHHHEEEEC
GIPTPAYRSFKDAASALAAADSLPFPFPVVVKPSREGSSVGVHICKTRDAYEAAVTDAAK
CCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHH
YAGTLLVEQFVKGREVQGGVLDDEALGVIEVRAAREFYDYDAKYKAGTGTQYLFPAPLPP
HHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEHHHHHHHCCCCCEECCCCCCEEECCCCCC
DQYARVNEVCLAAHQALGCSGGSRSDVIVTDGGDVFLLETNTLPGMTASSLLPKIAAGRG
HHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCEEEEECCCCCCCCHHHHHHHHHCCCC
IDFPALCERLLLGACLKA
CCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TPNRGAFTKDELKQKRVGVLLGGMSAERDVSLRTGEAVSGALRGLGYDVVEIDVGRDLP
CCCCCCCCHHHHHHHHHCEEEECCCCCCCCEEECCHHHHHHHHHCCCEEEEEECCCCCH
ARLAAEKVDVAWLAVHGRYGEDGCLQGLLESLFIPYTGSGVLASALGMDKVYAKQVYVAH
HHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHCHHHHHHHHEEEEC
GIPTPAYRSFKDAASALAAADSLPFPFPVVVKPSREGSSVGVHICKTRDAYEAAVTDAAK
CCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHH
YAGTLLVEQFVKGREVQGGVLDDEALGVIEVRAAREFYDYDAKYKAGTGTQYLFPAPLPP
HHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEHHHHHHHCCCCCEECCCCCCEEECCCCCC
DQYARVNEVCLAAHQALGCSGGSRSDVIVTDGGDVFLLETNTLPGMTASSLLPKIAAGRG
HHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCEEEEECCCCCCCCHHHHHHHHHCCCC
IDFPALCERLLLGACLKA
CCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA