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The map label for this gene is glnG [H]

Identifier: 108760840

GI number: 108760840

Start: 4404733

End: 4406163

Strand: Reverse

Name: glnG [H]

Synonym: MXAN_3692

Alternate gene names: 108760840

Gene position: 4406163-4404733 (Counterclockwise)

Preceding gene: 108763985

Following gene: 108759524

Centisome position: 48.21

GC content: 70.65

Gene sequence:

>1431_bases
ATGCGGCGACCAACCCCGCAAAGCGGCTGTGATATCGTCCGCGCCGTGGCGAGTCTGGGAGATGAGGATGGGGACGACGA
GCTGGTCCGCACCGATGCGTTGCCGGCCATTCGCGTCCAACGGGTACGTACCCGGCTGGTGGTGTTGTCGGGTCCGGACG
CTGGCCGCGACTGGCCGTTGATGCCGGGACGCTATCGCGTGGGTTCGGGCCGGGATGCGGACATCCTCCTGTCGGACCGG
GCGGTGTCGCGCCAGCACCTCATCCTCGATGTGAACGAGAGCGGCGTGCGCGCGGTGGACCCGGGCTCGCGCAATGGCTC
CTATTGCGAAGGCATGCGCTTCCAGGAGCTGGAGGTGCGCACGGGCGCGGTCCTCAAGCTGGGCACCTCCGAGCTGAAGC
TGATGCCAGAAAGCGAGCGGCCCCGGGCGCGTCCCTTGTCCGCGCGCAGCGCCTTCGGCTCACTGGTGGGAAACAGCCGC
CGCATGCGGGAGCTCTTCACGCTGCTGGAGCGGCTGTCCGCGGGCGAGTCGGACGTGCTCATCCAGGGTGAGACGGGCAC
GGGCAAGGAGCTGTGCGCGGAGGCCATCCACACGCACAGCCCTCGGGCCCGGGGGCCTTTCGTCATCGCCGACCTGGCGG
GCATCGCCCCCTCGCTGCTGGAGAGCGAGCTCTTTGGACACGTGAAAGGCGCCTTCACGGGCGCGCACTCCGACCGCGCG
GGCGCCTTCGAGCGCGCCCACGGCGGCACGCTCTTCCTGGACGAAGTCGGCGAACTGCCGCTGGAAGTCCAACCCCGCCT
GCTGCGCGCGCTGGAGCGCCGACAGGTGAAGCGCGTGGGCTCCAACGACTACCGCACCTTCGACGTGCGCGTCGTCGCGG
CCACGCACCAGGACCTGGAGGGCTCGGTGCAGGCCGGGCGCTTCCGTGGTGACCTCTTCCACCGGCTCGCGGTGCTCCGC
GTCAGCCTTCCGCCGCTGCGGGAGCGACTGGAAGACGTGCCGCTGCTCGTCGATACGGTGCTGCAGCGGATGGGGCGTCC
TCCCAGCGCACTGTCGGACCAGACGCGCGCCCTGCTCGCGCAGTACCCGTGGCCGGGCAACGTGCGCGAGCTGCGCAACG
TGGTGGACCGGGTGGTGAACCTGGGCGACGAGGCGCTGCCGGACATCCCGGACCTGCCTTCCCCCCCCGAGGACCCCACC
ACCCTGCCGCCGCCTTCGGACGACCCGGAGAGCACGCGCCCCATCTCCCTGGACCTCCCCTTCAAGGAAGCCAAGGACCG
CATCATCGAAGGCTTCGAGCGCGACTACCTGCGCGCCCTCATCGACCGCTGCGAGGGCAACGTGTCCCGCGCGGCCCGCG
AGGCGGGCATCGACCGCGTGTACCTGCGCAAGCTGCTGCGCAAGCACGGCCTGGACACGTCACCGGCGTAA

Upstream 100 bases:

>100_bases
GCCTACCCGCTCTTGAAGTTCATACCGGCCGAATCGTTCTCGCGGCGCGCTTGCCATCGCGGGTGACACCCCCTTTCAAA
CTTTACCGCATAACCCGGGA

Downstream 100 bases:

>100_bases
GTGGGTGTGGGCCTCCAAGCCCCCTGCTTTCGCGGCGACATCTGCAGGGTCGCGTGGCCGGATGTTTCCGGGCCGGGTCA
TTACGTGTCGAACGGCCCGG

Product: sigma-54 dependent trancsriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MRRPTPQSGCDIVRAVASLGDEDGDDELVRTDALPAIRVQRVRTRLVVLSGPDAGRDWPLMPGRYRVGSGRDADILLSDR
AVSRQHLILDVNESGVRAVDPGSRNGSYCEGMRFQELEVRTGAVLKLGTSELKLMPESERPRARPLSARSAFGSLVGNSR
RMRELFTLLERLSAGESDVLIQGETGTGKELCAEAIHTHSPRARGPFVIADLAGIAPSLLESELFGHVKGAFTGAHSDRA
GAFERAHGGTLFLDEVGELPLEVQPRLLRALERRQVKRVGSNDYRTFDVRVVAATHQDLEGSVQAGRFRGDLFHRLAVLR
VSLPPLRERLEDVPLLVDTVLQRMGRPPSALSDQTRALLAQYPWPGNVRELRNVVDRVVNLGDEALPDIPDLPSPPEDPT
TLPPPSDDPESTRPISLDLPFKEAKDRIIEGFERDYLRALIDRCEGNVSRAAREAGIDRVYLRKLLRKHGLDTSPA

Sequences:

>Translated_476_residues
MRRPTPQSGCDIVRAVASLGDEDGDDELVRTDALPAIRVQRVRTRLVVLSGPDAGRDWPLMPGRYRVGSGRDADILLSDR
AVSRQHLILDVNESGVRAVDPGSRNGSYCEGMRFQELEVRTGAVLKLGTSELKLMPESERPRARPLSARSAFGSLVGNSR
RMRELFTLLERLSAGESDVLIQGETGTGKELCAEAIHTHSPRARGPFVIADLAGIAPSLLESELFGHVKGAFTGAHSDRA
GAFERAHGGTLFLDEVGELPLEVQPRLLRALERRQVKRVGSNDYRTFDVRVVAATHQDLEGSVQAGRFRGDLFHRLAVLR
VSLPPLRERLEDVPLLVDTVLQRMGRPPSALSDQTRALLAQYPWPGNVRELRNVVDRVVNLGDEALPDIPDLPSPPEDPT
TLPPPSDDPESTRPISLDLPFKEAKDRIIEGFERDYLRALIDRCEGNVSRAAREAGIDRVYLRKLLRKHGLDTSPA
>Mature_476_residues
MRRPTPQSGCDIVRAVASLGDEDGDDELVRTDALPAIRVQRVRTRLVVLSGPDAGRDWPLMPGRYRVGSGRDADILLSDR
AVSRQHLILDVNESGVRAVDPGSRNGSYCEGMRFQELEVRTGAVLKLGTSELKLMPESERPRARPLSARSAFGSLVGNSR
RMRELFTLLERLSAGESDVLIQGETGTGKELCAEAIHTHSPRARGPFVIADLAGIAPSLLESELFGHVKGAFTGAHSDRA
GAFERAHGGTLFLDEVGELPLEVQPRLLRALERRQVKRVGSNDYRTFDVRVVAATHQDLEGSVQAGRFRGDLFHRLAVLR
VSLPPLRERLEDVPLLVDTVLQRMGRPPSALSDQTRALLAQYPWPGNVRELRNVVDRVVNLGDEALPDIPDLPSPPEDPT
TLPPPSDDPESTRPISLDLPFKEAKDRIIEGFERDYLRALIDRCEGNVSRAAREAGIDRVYLRKLLRKHGLDTSPA

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=354, Percent_Identity=42.3728813559322, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1788550, Length=347, Percent_Identity=40.0576368876081, Blast_Score=242, Evalue=5e-65,
Organism=Escherichia coli, GI1790437, Length=328, Percent_Identity=42.6829268292683, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI87082152, Length=328, Percent_Identity=43.5975609756098, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI1788905, Length=326, Percent_Identity=40.7975460122699, Blast_Score=222, Evalue=3e-59,
Organism=Escherichia coli, GI1789087, Length=259, Percent_Identity=46.7181467181467, Blast_Score=218, Evalue=9e-58,
Organism=Escherichia coli, GI87082117, Length=234, Percent_Identity=50.8547008547009, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1789233, Length=329, Percent_Identity=37.9939209726444, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI1786524, Length=262, Percent_Identity=46.9465648854962, Blast_Score=211, Evalue=8e-56,
Organism=Escherichia coli, GI87081872, Length=329, Percent_Identity=39.209726443769, Blast_Score=199, Evalue=5e-52,
Organism=Escherichia coli, GI1787583, Length=358, Percent_Identity=34.0782122905028, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI87081858, Length=298, Percent_Identity=30.8724832214765, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1789828, Length=220, Percent_Identity=37.2727272727273, Blast_Score=122, Evalue=6e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 52486; Mature: 52486

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS50006 FHA_DOMAIN ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRPTPQSGCDIVRAVASLGDEDGDDELVRTDALPAIRVQRVRTRLVVLSGPDAGRDWPL
CCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHEEEEEEECCCCCCCCCC
MPGRYRVGSGRDADILLSDRAVSRQHLILDVNESGVRAVDPGSRNGSYCEGMRFQELEVR
CCCCEECCCCCCCEEEECCCCCCCCEEEEEECCCCCEEECCCCCCCCHHCCCCCCEEECC
TGAVLKLGTSELKLMPESERPRARPLSARSAFGSLVGNSRRMRELFTLLERLSAGESDVL
CCCEEEECCHHHEECCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEE
IQGETGTGKELCAEAIHTHSPRARGPFVIADLAGIAPSLLESELFGHVKGAFTGAHSDRA
EECCCCCCHHHHHHHHHCCCCCCCCCEEEEEHHHCCHHHHHHHHHHHHHHHCCCCCCCCC
GAFERAHGGTLFLDEVGELPLEVQPRLLRALERRQVKRVGSNDYRTFDVRVVAATHQDLE
CCHHHCCCCEEEEHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCC
GSVQAGRFRGDLFHRLAVLRVSLPPLRERLEDVPLLVDTVLQRMGRPPSALSDQTRALLA
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHH
QYPWPGNVRELRNVVDRVVNLGDEALPDIPDLPSPPEDPTTLPPPSDDPESTRPISLDLP
CCCCCCCHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCC
FKEAKDRIIEGFERDYLRALIDRCEGNVSRAAREAGIDRVYLRKLLRKHGLDTSPA
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRRPTPQSGCDIVRAVASLGDEDGDDELVRTDALPAIRVQRVRTRLVVLSGPDAGRDWPL
CCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHEEEEEEECCCCCCCCCC
MPGRYRVGSGRDADILLSDRAVSRQHLILDVNESGVRAVDPGSRNGSYCEGMRFQELEVR
CCCCEECCCCCCCEEEECCCCCCCCEEEEEECCCCCEEECCCCCCCCHHCCCCCCEEECC
TGAVLKLGTSELKLMPESERPRARPLSARSAFGSLVGNSRRMRELFTLLERLSAGESDVL
CCCEEEECCHHHEECCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEE
IQGETGTGKELCAEAIHTHSPRARGPFVIADLAGIAPSLLESELFGHVKGAFTGAHSDRA
EECCCCCCHHHHHHHHHCCCCCCCCCEEEEEHHHCCHHHHHHHHHHHHHHHCCCCCCCCC
GAFERAHGGTLFLDEVGELPLEVQPRLLRALERRQVKRVGSNDYRTFDVRVVAATHQDLE
CCHHHCCCCEEEEHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCC
GSVQAGRFRGDLFHRLAVLRVSLPPLRERLEDVPLLVDTVLQRMGRPPSALSDQTRALLA
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHH
QYPWPGNVRELRNVVDRVVNLGDEALPDIPDLPSPPEDPTTLPPPSDDPESTRPISLDLP
CCCCCCCHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCC
FKEAKDRIIEGFERDYLRALIDRCEGNVSRAAREAGIDRVYLRKLLRKHGLDTSPA
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]