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The map label for this gene is fixI [H]

Identifier: 108760534

GI number: 108760534

Start: 6892003

End: 6894531

Strand: Reverse

Name: fixI [H]

Synonym: MXAN_5543

Alternate gene names: 108760534

Gene position: 6894531-6892003 (Counterclockwise)

Preceding gene: 108757390

Following gene: 108763666

Centisome position: 75.43

GC content: 72.48

Gene sequence:

>2529_bases
ATGCCTGCCTCCACGACATCGGAGCCTGCTCCGACCGCCTGTCTCCACTGCGGCAGCCCCGTTCCTTCGGGGGGCGCGGC
GCGCGACTTCTGCTGTGTGGGGTGCGAGGCCGTGCACGGCCTGCTCGTGGAGCAGGGGCTCACGCGCTACTACCAGCTCG
CGCAAGGAAAGACGGCGCCCGCGCCTGAGCCGCGCAAGGAACGCGCGTTCGCGTGGCTGGAGCCGCTCATGAGTCGCGCG
GAGTCGACTCCAGGCCCCTTGTGCGCGCTCGAACTGGACGTGCAGGGCATCCACTGCGCCGCATGCGTGTGGCTGATGAA
CGAGCTGTTCCGCCGGCAGCCGGGCGGGGCGGGTCTGACGGTGGACCCGGCCCTAGGCAAGGTGCGGATGCTGTGGCGGC
GCGGCGCCTTCGACGTGGCGGAGTTCCTTCGCGGCGTGGAGGGCTTTGGCTACCTCTTCGGACCCAGCCGCAAGCGCCCG
GAGCGCACGAGCCTGGACCTGCCCATCCGCCTGGGCATCTGCGCCGCGCTGTCGATGAACGTGATGCTGTTCTCGGTGAG
CTTCTACGTGGGGCTCACGCCGGAGGATGGCGATGTCTTCCGTCTCTTCACACGGCTGAGTCTGTGGCTGTCGTCCGCGG
TCGTGGTGGTGGGCGGCTGGCCCTTCTTCCGCTCCGCGCTTCAGGGCTTGCGGCGCGGCGTGCTGCACCTGGACCTGCCC
ATTGCCCTGGGCATCCTGCTGGTGTTCGGCATGTCGCTGGCGCAGGCGCGCGGAGGGCGCGGCGACCTGGCGTACTTCGA
CACGCTCAACACCTTCGTCACGCTGATGCTGGTGGGACGGTGGCTCCAGCAGCGTGTGTTGGAGCGCAACCGGCGCTTCC
TGCTGGATGACGACGGGGCGGAAGGGCTCTTCGTCCGCAGGGAAGAGGGCGCGCGGCTCGCCACCGTGCGCGCCGCCGAG
GTGGCCGACGGCGACGTGCTGGTGATTGCGCCGGGGGACCTGGTGCCGGTGGACGCGGTGCTGCTCGATTCGGGGGCCCG
CGTGTCCACGGATTGGATTACGGGCGAGCCCGGCGAGCGCGCGGTGGGGCAGGGGGGGGCGTTGCCCGCGGGCGCGTTCA
ACGCCGGGCGCGAGGCCGTGCGCATCCAGGCGAAGCAGGCGTTCACCGACTCTCCGCTGGTGGCGTTGCTCCGCCGGGCG
CCCACGGCCGCAGGGGGGCCCGCGCTGCACACCCGCTTCTGGGACCGCGTGTCGCGCCGCTGGGTCGTCACGGTGCTCTT
CGTCTCCGCGTTGGGGCTCGCGCTGTGGTGGCCCGCCGGGCCGGACAAGGCGCTGGAGGTGGCGGTGGCGCTGCTGGTGG
TGACGTGCCCGTGCGCCATTGGCATCGCCACGCCGCTGGCCTACGAGTTGGTCCAGGCGCGCCTGCGCCGCGGCGGCTTC
TTCATCCGGAGCACGGACCTGCTGGACCGGCTGCCGCGCGTGCGAAAGGTGCTCTTCGACAAGACGGGGACGCTGACGCT
GGGGCGGCTGGAGTTGGTGGACCGCGCCGCCGTGGCGGGGTTGACGGCCGCGTCGCGCGACATCGCCTTCGACCTGGTGT
CGCGCAGCAACCACCCCGCCAGTCGCTGCCTCGCGGCCGCGCTGGCTCGGGAAGGCGCGCGCTTCAACTCGGAGGCGCGT
GTGTCGGAGCTCGCGGGCCAGGGGCTCGAGCTGAATCGAGAAGGCGTGCGCTGGCGGCTGGGCCGCGCGGACTGGGCCAC
CGAGGCGGTGGACACAGGGCATCCGGAGCGCGAGCCCACGGATGGCGCGTCGTGTGCGGGGGGCAGCCCCGCGAGCCGCC
ACTCCAGGCCGAGTGGGCGTGTGGAGGTCCTCCTCTCCGGTTCGGGCCTCGCCGCGGGCCCGGTGCTGACCCGTGACGGC
GTGCCAGTGGCCTTCTTCCAGCTCCGGGAGTCGGTACGTCCCGACGCGCGCCGTGAGGTCCAGGCGCTCCAGGGAGAGGG
CCGCGAGGTGTGGCTCATCTCCGGGGACAGCCACGGCCGCGTGAGTGACATGGCCGCCGCGCTGGGCATCCCCACCCAGC
ACACCCTGAGCGGCCAGCGTCCCGAGGACAAGGCGGAGGCTGTCGCGAAGCTGGACGCGGCGGACACGCTGTACCTGGGT
GACGGCGTCAACGACAGCCTCGCCTTCGAGCGAGCCCTCTGCGCGGGTACGCCCGCCATCGACCGGCCGGTGATGCCCGG
CAAGAGCGACTTCTTCCTCCTGGGCGAAGGCCTGGGTGCCATCCGCGAGGCACTGCGGTTGTCGGCCCGGTTGCGCCAGG
TGGTGCGCCGGCTGCTGCTCTTGGCGGTGAGCTACAACGTGGTGGCCGTCGCCGTGTGCCTCGCGGGTTGGATGACGCCC
CTGCGCGCCGCCGTGGCCATGCCCGCCACCAGTCTGGCCACCGTGCTCTTCACGGTGTGGTGGCTGTCCGCCTCACGTGA
GCGCGCCGCCGCCTCGCCCACGCCGCCGCTGCGGGAGGTGCCGGCATGA

Upstream 100 bases:

>100_bases
GGGTTCGGAGGTGTGCAGCAGCGAGGCGAGTGCGGCCGGTTCAGGCGACATGAACGCGGTGCATCGCAACAGGCATGCCG
TTCGCAAGGTGACATCGTGA

Downstream 100 bases:

>100_bases
ACGTCCTCGTCCTCCAGGTCTTTGTGAGCCTGATGCTCGTCGCCAGTTCGGTGCTGTTGTTCGTCTACAGCGTGCGCCAC
CGCGACCACGAGCACGCCGA

Product: P-type ATPase

Products: ADP; Orthophosphate. [C]

Alternate protein names: E1-E2 type cation ATPase FixI [H]

Number of amino acids: Translated: 842; Mature: 841

Protein sequence:

>842_residues
MPASTTSEPAPTACLHCGSPVPSGGAARDFCCVGCEAVHGLLVEQGLTRYYQLAQGKTAPAPEPRKERAFAWLEPLMSRA
ESTPGPLCALELDVQGIHCAACVWLMNELFRRQPGGAGLTVDPALGKVRMLWRRGAFDVAEFLRGVEGFGYLFGPSRKRP
ERTSLDLPIRLGICAALSMNVMLFSVSFYVGLTPEDGDVFRLFTRLSLWLSSAVVVVGGWPFFRSALQGLRRGVLHLDLP
IALGILLVFGMSLAQARGGRGDLAYFDTLNTFVTLMLVGRWLQQRVLERNRRFLLDDDGAEGLFVRREEGARLATVRAAE
VADGDVLVIAPGDLVPVDAVLLDSGARVSTDWITGEPGERAVGQGGALPAGAFNAGREAVRIQAKQAFTDSPLVALLRRA
PTAAGGPALHTRFWDRVSRRWVVTVLFVSALGLALWWPAGPDKALEVAVALLVVTCPCAIGIATPLAYELVQARLRRGGF
FIRSTDLLDRLPRVRKVLFDKTGTLTLGRLELVDRAAVAGLTAASRDIAFDLVSRSNHPASRCLAAALAREGARFNSEAR
VSELAGQGLELNREGVRWRLGRADWATEAVDTGHPEREPTDGASCAGGSPASRHSRPSGRVEVLLSGSGLAAGPVLTRDG
VPVAFFQLRESVRPDARREVQALQGEGREVWLISGDSHGRVSDMAAALGIPTQHTLSGQRPEDKAEAVAKLDAADTLYLG
DGVNDSLAFERALCAGTPAIDRPVMPGKSDFFLLGEGLGAIREALRLSARLRQVVRRLLLLAVSYNVVAVAVCLAGWMTP
LRAAVAMPATSLATVLFTVWWLSASRERAAASPTPPLREVPA

Sequences:

>Translated_842_residues
MPASTTSEPAPTACLHCGSPVPSGGAARDFCCVGCEAVHGLLVEQGLTRYYQLAQGKTAPAPEPRKERAFAWLEPLMSRA
ESTPGPLCALELDVQGIHCAACVWLMNELFRRQPGGAGLTVDPALGKVRMLWRRGAFDVAEFLRGVEGFGYLFGPSRKRP
ERTSLDLPIRLGICAALSMNVMLFSVSFYVGLTPEDGDVFRLFTRLSLWLSSAVVVVGGWPFFRSALQGLRRGVLHLDLP
IALGILLVFGMSLAQARGGRGDLAYFDTLNTFVTLMLVGRWLQQRVLERNRRFLLDDDGAEGLFVRREEGARLATVRAAE
VADGDVLVIAPGDLVPVDAVLLDSGARVSTDWITGEPGERAVGQGGALPAGAFNAGREAVRIQAKQAFTDSPLVALLRRA
PTAAGGPALHTRFWDRVSRRWVVTVLFVSALGLALWWPAGPDKALEVAVALLVVTCPCAIGIATPLAYELVQARLRRGGF
FIRSTDLLDRLPRVRKVLFDKTGTLTLGRLELVDRAAVAGLTAASRDIAFDLVSRSNHPASRCLAAALAREGARFNSEAR
VSELAGQGLELNREGVRWRLGRADWATEAVDTGHPEREPTDGASCAGGSPASRHSRPSGRVEVLLSGSGLAAGPVLTRDG
VPVAFFQLRESVRPDARREVQALQGEGREVWLISGDSHGRVSDMAAALGIPTQHTLSGQRPEDKAEAVAKLDAADTLYLG
DGVNDSLAFERALCAGTPAIDRPVMPGKSDFFLLGEGLGAIREALRLSARLRQVVRRLLLLAVSYNVVAVAVCLAGWMTP
LRAAVAMPATSLATVLFTVWWLSASRERAAASPTPPLREVPA
>Mature_841_residues
PASTTSEPAPTACLHCGSPVPSGGAARDFCCVGCEAVHGLLVEQGLTRYYQLAQGKTAPAPEPRKERAFAWLEPLMSRAE
STPGPLCALELDVQGIHCAACVWLMNELFRRQPGGAGLTVDPALGKVRMLWRRGAFDVAEFLRGVEGFGYLFGPSRKRPE
RTSLDLPIRLGICAALSMNVMLFSVSFYVGLTPEDGDVFRLFTRLSLWLSSAVVVVGGWPFFRSALQGLRRGVLHLDLPI
ALGILLVFGMSLAQARGGRGDLAYFDTLNTFVTLMLVGRWLQQRVLERNRRFLLDDDGAEGLFVRREEGARLATVRAAEV
ADGDVLVIAPGDLVPVDAVLLDSGARVSTDWITGEPGERAVGQGGALPAGAFNAGREAVRIQAKQAFTDSPLVALLRRAP
TAAGGPALHTRFWDRVSRRWVVTVLFVSALGLALWWPAGPDKALEVAVALLVVTCPCAIGIATPLAYELVQARLRRGGFF
IRSTDLLDRLPRVRKVLFDKTGTLTLGRLELVDRAAVAGLTAASRDIAFDLVSRSNHPASRCLAAALAREGARFNSEARV
SELAGQGLELNREGVRWRLGRADWATEAVDTGHPEREPTDGASCAGGSPASRHSRPSGRVEVLLSGSGLAAGPVLTRDGV
PVAFFQLRESVRPDARREVQALQGEGREVWLISGDSHGRVSDMAAALGIPTQHTLSGQRPEDKAEAVAKLDAADTLYLGD
GVNDSLAFERALCAGTPAIDRPVMPGKSDFFLLGEGLGAIREALRLSARLRQVVRRLLLLAVSYNVVAVAVCLAGWMTPL
RAAVAMPATSLATVLFTVWWLSASRERAAASPTPPLREVPA

Specific function: FixI is a pump of a specific cation involved in symbiotic nitrogen fixation. The four proteins FixG, FixH, FixI, and FixS may participate in a membrane-bound complex coupling the FixI cation pump with a redox process catalyzed by FixG [H]

COG id: COG2217

COG function: function code P; Cation transport ATPase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HMA domain [H]

Homologues:

Organism=Homo sapiens, GI55743071, Length=828, Percent_Identity=22.8260869565217, Blast_Score=157, Evalue=5e-38,
Organism=Homo sapiens, GI115529486, Length=829, Percent_Identity=20.9891435464415, Blast_Score=134, Evalue=3e-31,
Organism=Escherichia coli, GI1786691, Length=805, Percent_Identity=25.4658385093168, Blast_Score=140, Evalue=4e-34,
Organism=Escherichia coli, GI1789879, Length=643, Percent_Identity=27.2161741835148, Blast_Score=97, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17556548, Length=668, Percent_Identity=21.8562874251497, Blast_Score=98, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6319772, Length=628, Percent_Identity=23.0891719745223, Blast_Score=136, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6320475, Length=674, Percent_Identity=20.919881305638, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI221329854, Length=603, Percent_Identity=21.7247097844113, Blast_Score=99, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008250
- InterPro:   IPR006403
- InterPro:   IPR000695
- InterPro:   IPR006416
- InterPro:   IPR001757
- InterPro:   IPR018303
- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR017969
- InterPro:   IPR006121
- InterPro:   IPR000150 [H]

Pfam domain/function: PF00122 E1-E2_ATPase; PF00702 Hydrolase [H]

EC number: 3.6.3.4 [C]

Molecular weight: Translated: 90283; Mature: 90152

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: PS00154 ATPASE_E1_E2 ; PS50846 HMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPASTTSEPAPTACLHCGSPVPSGGAARDFCCVGCEAVHGLLVEQGLTRYYQLAQGKTAP
CCCCCCCCCCCCHHEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
APEPRKERAFAWLEPLMSRAESTPGPLCALELDVQGIHCAACVWLMNELFRRQPGGAGLT
CCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCE
VDPALGKVRMLWRRGAFDVAEFLRGVEGFGYLFGPSRKRPERTSLDLPIRLGICAALSMN
ECCHHHHHHHHHHCCCHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHH
VMLFSVSFYVGLTPEDGDVFRLFTRLSLWLSSAVVVVGGWPFFRSALQGLRRGVLHLDLP
HHEEEEEEEEEECCCCCHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHCCEEECHH
IALGILLVFGMSLAQARGGRGDLAYFDTLNTFVTLMLVGRWLQQRVLERNRRFLLDDDGA
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCC
EGLFVRREEGARLATVRAAEVADGDVLVIAPGDLVPVDAVLLDSGARVSTDWITGEPGER
CCEEEECCCCCEEEEEEEHEECCCCEEEECCCCCCCCCHHEECCCCEEEECCCCCCCCCH
AVGQGGALPAGAFNAGREAVRIQAKQAFTDSPLVALLRRAPTAAGGPALHTRFWDRVSRR
HCCCCCCCCCCCCCCCCHHEEEEHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
WVVTVLFVSALGLALWWPAGPDKALEVAVALLVVTCPCAIGIATPLAYELVQARLRRGGF
HHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCE
FIRSTDLLDRLPRVRKVLFDKTGTLTLGRLELVDRAAVAGLTAASRDIAFDLVSRSNHPA
EEEEHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCH
SRCLAAALAREGARFNSEARVSELAGQGLELNREGVRWRLGRADWATEAVDTGHPEREPT
HHHHHHHHHHCCCCCCCHHHHHHHCCCCCEECCCCCEEEECCCHHHHHHHHCCCCCCCCC
DGASCAGGSPASRHSRPSGRVEVLLSGSGLAAGPVLTRDGVPVAFFQLRESVRPDARREV
CCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEECCCCCHHHHHHHHHCCCCHHHHH
QALQGEGREVWLISGDSHGRVSDMAAALGIPTQHTLSGQRPEDKAEAVAKLDAADTLYLG
HHHHCCCCEEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEC
DGVNDSLAFERALCAGTPAIDRPVMPGKSDFFLLGEGLGAIREALRLSARLRQVVRRLLL
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
LAVSYNVVAVAVCLAGWMTPLRAAVAMPATSLATVLFTVWWLSASRERAAASPTPPLREV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCC
PA
CC
>Mature Secondary Structure 
PASTTSEPAPTACLHCGSPVPSGGAARDFCCVGCEAVHGLLVEQGLTRYYQLAQGKTAP
CCCCCCCCCCCHHEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
APEPRKERAFAWLEPLMSRAESTPGPLCALELDVQGIHCAACVWLMNELFRRQPGGAGLT
CCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCE
VDPALGKVRMLWRRGAFDVAEFLRGVEGFGYLFGPSRKRPERTSLDLPIRLGICAALSMN
ECCHHHHHHHHHHCCCHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHH
VMLFSVSFYVGLTPEDGDVFRLFTRLSLWLSSAVVVVGGWPFFRSALQGLRRGVLHLDLP
HHEEEEEEEEEECCCCCHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHCCEEECHH
IALGILLVFGMSLAQARGGRGDLAYFDTLNTFVTLMLVGRWLQQRVLERNRRFLLDDDGA
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCC
EGLFVRREEGARLATVRAAEVADGDVLVIAPGDLVPVDAVLLDSGARVSTDWITGEPGER
CCEEEECCCCCEEEEEEEHEECCCCEEEECCCCCCCCCHHEECCCCEEEECCCCCCCCCH
AVGQGGALPAGAFNAGREAVRIQAKQAFTDSPLVALLRRAPTAAGGPALHTRFWDRVSRR
HCCCCCCCCCCCCCCCCHHEEEEHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
WVVTVLFVSALGLALWWPAGPDKALEVAVALLVVTCPCAIGIATPLAYELVQARLRRGGF
HHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCE
FIRSTDLLDRLPRVRKVLFDKTGTLTLGRLELVDRAAVAGLTAASRDIAFDLVSRSNHPA
EEEEHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCH
SRCLAAALAREGARFNSEARVSELAGQGLELNREGVRWRLGRADWATEAVDTGHPEREPT
HHHHHHHHHHCCCCCCCHHHHHHHCCCCCEECCCCCEEEECCCHHHHHHHHCCCCCCCCC
DGASCAGGSPASRHSRPSGRVEVLLSGSGLAAGPVLTRDGVPVAFFQLRESVRPDARREV
CCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEECCCCCHHHHHHHHHCCCCHHHHH
QALQGEGREVWLISGDSHGRVSDMAAALGIPTQHTLSGQRPEDKAEAVAKLDAADTLYLG
HHHHCCCCEEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEC
DGVNDSLAFERALCAGTPAIDRPVMPGKSDFFLLGEGLGAIREALRLSARLRQVVRRLLL
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
LAVSYNVVAVAVCLAGWMTPLRAAVAMPATSLATVLFTVWWLSASRERAAASPTPPLREV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCC
PA
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Cu [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; H2O [C]

Specific reaction: ATP + H2O = ADP + Orthophosphate. [C]

General reaction: Phosphorous acid anhydride hydrolysis [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2536685; 11481432 [H]