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The map label for this gene is menD

Identifier: 108759318

GI number: 108759318

Start: 4108458

End: 4110227

Strand: Direct

Name: menD

Synonym: MXAN_3528

Alternate gene names: 108759318

Gene position: 4108458-4110227 (Clockwise)

Preceding gene: 108758468

Following gene: 108760127

Centisome position: 44.95

GC content: 72.82

Gene sequence:

>1770_bases
ATGTCGTCCGACGCTAACCTCAACGTGTTGTGGGCGCGCGCGTTGCTGGAAGAGCTCGTGCGCGGTGGGGTTCGTCACGC
GGTGGTGTGTCCGGGTTCCCGGTCATCGCCCCTGGCCCTGGCCTGTGCCGGGACGGAGGGGCTGCGCACGTGGTCGGTCA
TCGACGAGCGCAGCGCGGGCTTCTTCGCCCTGGGGCTCGCGAAGCAGTCGCGCGCACCGGCCGTGGTGGTGGCGACCAGC
GGCACCGCAGGCGCGCACTTCTACCCGGCGGTCATCGAGGCCGCGCTGTCTCACGTGCCCCTGGTGGTGCTGACGGCGGA
CCGGCCCCTGGAGCTTCAGGGCTGGGGCGCGCCGCAGACCGTGCCACAGGCGCGCTTCTTCGGAGAGCACGCGCGCTTCT
ACGCGGACCTCGGTCAGCCCGAGGCGCATGACGCGGCGTTGATTCACATGCGTGCCACCGTTGCCCGCGCGGTCGTCAGC
GCGTGGCGCGCGCCGCGGGGCGCCGTGCAGCTCAACGTCCCATTCCGCGAGCCGCTGGCCCCCATCGCCGAGCCCTTCCC
CGAGGCCTCCCTGAGCGCGCTCGCGAAGTCGGGCCGCGCCGGTGCGCCGCTGACGCGCATCGTCCCGCCCGAGCCGGTGC
CGGACGCGTCCACCCTGGACGAGGTGCGCCGCCGGATTGCCGCGACCACTCGCGGCGTCATCGTGTGTGGCCCGCGAGAT
GAGATGGATGGATTCGCGGCGGCCATCAGCGCGCTGTCGCAGGCCACGGGCTATCCCGTGCTGGCCGAGTCCACCTCGCA
GGCCCGCTATGGCGGCGGCCCGCTCACGCTGTCCTATTACGACGCGATGTTGCGGCATGCGCCGTTCGCGAAGGCGCACC
GTCCGGAGCTCGTGTTGCGCTTCGGGGGCGGGCTCACGCCGAAGGTGCCTCAGCAGTGGCTGGACGGCTCGGGCGCGGAC
ACCGTCCTCTTCAGTGACGGCGGCGGGTTGTTCGACCCGGCGCACCGTGCGTCCACGGTGGTGGAGGGCTCGGCGGTGGC
GGCCTGTGAGGCCCTGACGCGAGGACTCGCGCGCGGCGTGGGGGCGTGGGCACGAGGCTTCCTGGCCGCGGAGCAACGGG
TGCGCACGGCGCTGGAGGCCGCGTTCGCGGAGCAGCCCGATGTGCTCTCCGAGCCGCGTATCGCTCGCGAGGTGGTGGCG
GCGCTTCCCGCGGGCGCGCCGCTCTTCGTATCCAGCAGCATGCCCATCCGCGACCTGGATGCCTTCGCTCCGGCGGGCAC
GGTGCCGCTGCGGGTGCTGGCCAATCGGGGGGCCAACGGAATCGACGGCATTGTCTCCAGCGCGCTCGGCATGGCGGCCG
CTGCGGGCCGCTCCGCGGTGCTGCTCACGGGGGACCTGGCGTTGCTGCATGACGTGGGAGCCTTCGTCACCGCGTCGCGC
ACGCGAGTGCCGCTCACGGTGGTGGCGGTGAACAACGATGGGGGCGGCATCTTCTCGTTCCTCCCCATCGCCCAGGTCGC
GCCGCGGGACACGTTCGAGACGCTCTTCGGTACGCCGCACGGTGTGGACCTGTCCCACGCGGCGGCGCTGGGCGGCGCCC
GGCTCCACCGGCCGGAGACGCCCGTCGCGCTCAGGTCGGCCGTGCGCGAGGGCCTGGAGGGCGGGCTCCACCTGGTGGAA
GTGCGCGTGGACCGCAACGCGAATGTGGATGAGCACCGGCGGCTGTTCGCTCGAATGGCGGCCTCACTGGGAGAAGGACC
ATGGGCGTGA

Upstream 100 bases:

>100_bases
TTCGTCGGCGTGGGGCTGGTGGCTGGCTCCAACGCGGACTCGGAGTGGCGGGAGACGGAGATGAAGAGCCTGGCCATGTT
GCGGGCCTTGGGAGGCGGGG

Downstream 100 bases:

>100_bases
AGCTGGCTTACGAGACGTGGGGTGAGGGCTCCCGTCCGCTCGTGTTGCTGCACGGCTTCACCGGCAGCCGCAGGGCCTTC
GACGGGCTGCGTCCGCTCCT

Product: 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylic acid synthase/2-oxoglutarate decarboxylase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD

Number of amino acids: Translated: 589; Mature: 588

Protein sequence:

>589_residues
MSSDANLNVLWARALLEELVRGGVRHAVVCPGSRSSPLALACAGTEGLRTWSVIDERSAGFFALGLAKQSRAPAVVVATS
GTAGAHFYPAVIEAALSHVPLVVLTADRPLELQGWGAPQTVPQARFFGEHARFYADLGQPEAHDAALIHMRATVARAVVS
AWRAPRGAVQLNVPFREPLAPIAEPFPEASLSALAKSGRAGAPLTRIVPPEPVPDASTLDEVRRRIAATTRGVIVCGPRD
EMDGFAAAISALSQATGYPVLAESTSQARYGGGPLTLSYYDAMLRHAPFAKAHRPELVLRFGGGLTPKVPQQWLDGSGAD
TVLFSDGGGLFDPAHRASTVVEGSAVAACEALTRGLARGVGAWARGFLAAEQRVRTALEAAFAEQPDVLSEPRIAREVVA
ALPAGAPLFVSSSMPIRDLDAFAPAGTVPLRVLANRGANGIDGIVSSALGMAAAAGRSAVLLTGDLALLHDVGAFVTASR
TRVPLTVVAVNNDGGGIFSFLPIAQVAPRDTFETLFGTPHGVDLSHAAALGGARLHRPETPVALRSAVREGLEGGLHLVE
VRVDRNANVDEHRRLFARMAASLGEGPWA

Sequences:

>Translated_589_residues
MSSDANLNVLWARALLEELVRGGVRHAVVCPGSRSSPLALACAGTEGLRTWSVIDERSAGFFALGLAKQSRAPAVVVATS
GTAGAHFYPAVIEAALSHVPLVVLTADRPLELQGWGAPQTVPQARFFGEHARFYADLGQPEAHDAALIHMRATVARAVVS
AWRAPRGAVQLNVPFREPLAPIAEPFPEASLSALAKSGRAGAPLTRIVPPEPVPDASTLDEVRRRIAATTRGVIVCGPRD
EMDGFAAAISALSQATGYPVLAESTSQARYGGGPLTLSYYDAMLRHAPFAKAHRPELVLRFGGGLTPKVPQQWLDGSGAD
TVLFSDGGGLFDPAHRASTVVEGSAVAACEALTRGLARGVGAWARGFLAAEQRVRTALEAAFAEQPDVLSEPRIAREVVA
ALPAGAPLFVSSSMPIRDLDAFAPAGTVPLRVLANRGANGIDGIVSSALGMAAAAGRSAVLLTGDLALLHDVGAFVTASR
TRVPLTVVAVNNDGGGIFSFLPIAQVAPRDTFETLFGTPHGVDLSHAAALGGARLHRPETPVALRSAVREGLEGGLHLVE
VRVDRNANVDEHRRLFARMAASLGEGPWA
>Mature_588_residues
SSDANLNVLWARALLEELVRGGVRHAVVCPGSRSSPLALACAGTEGLRTWSVIDERSAGFFALGLAKQSRAPAVVVATSG
TAGAHFYPAVIEAALSHVPLVVLTADRPLELQGWGAPQTVPQARFFGEHARFYADLGQPEAHDAALIHMRATVARAVVSA
WRAPRGAVQLNVPFREPLAPIAEPFPEASLSALAKSGRAGAPLTRIVPPEPVPDASTLDEVRRRIAATTRGVIVCGPRDE
MDGFAAAISALSQATGYPVLAESTSQARYGGGPLTLSYYDAMLRHAPFAKAHRPELVLRFGGGLTPKVPQQWLDGSGADT
VLFSDGGGLFDPAHRASTVVEGSAVAACEALTRGLARGVGAWARGFLAAEQRVRTALEAAFAEQPDVLSEPRIAREVVAA
LPAGAPLFVSSSMPIRDLDAFAPAGTVPLRVLANRGANGIDGIVSSALGMAAAAGRSAVLLTGDLALLHDVGAFVTASRT
RVPLTVVAVNNDGGGIFSFLPIAQVAPRDTFETLFGTPHGVDLSHAAALGGARLHRPETPVALRSAVREGLEGGLHLVEV
RVDRNANVDEHRRLFARMAASLGEGPWA

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily

Homologues:

Organism=Escherichia coli, GI1788599, Length=558, Percent_Identity=32.9749103942652, Blast_Score=231, Evalue=1e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MEND_MYXXD (Q1D6K1)

Other databases:

- EMBL:   CP000113
- RefSeq:   YP_631722.1
- ProteinModelPortal:   Q1D6K1
- STRING:   Q1D6K1
- GeneID:   4104121
- GenomeReviews:   CP000113_GR
- KEGG:   mxa:MXAN_3528
- TIGR:   MXAN_3528
- eggNOG:   COG1165
- HOGENOM:   HBG634405
- OMA:   FYHDLNG
- PhylomeDB:   Q1D6K1
- BioCyc:   MXAN246197:MXAN_3528-MONOMER
- HAMAP:   MF_01659
- InterPro:   IPR004433
- InterPro:   IPR012001
- InterPro:   IPR011766
- TIGRFAMs:   TIGR00173

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N

EC number: =2.2.1.9

Molecular weight: Translated: 61439; Mature: 61307

Theoretical pI: Translated: 7.48; Mature: 7.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSDANLNVLWARALLEELVRGGVRHAVVCPGSRSSPLALACAGTEGLRTWSVIDERSAG
CCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEECCCCCEEEHHHHCCCCC
FFALGLAKQSRAPAVVVATSGTAGAHFYPAVIEAALSHVPLVVLTADRPLELQGWGAPQT
EEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCEEEEECCCCEEEECCCCCCC
VPQARFFGEHARFYADLGQPEAHDAALIHMRATVARAVVSAWRAPRGAVQLNVPFREPLA
CCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCC
PIAEPFPEASLSALAKSGRAGAPLTRIVPPEPVPDASTLDEVRRRIAATTRGVIVCGPRD
HHHCCCCHHHHHHHHHCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCEEEECCCH
EMDGFAAAISALSQATGYPVLAESTSQARYGGGPLTLSYYDAMLRHAPFAKAHRPELVLR
HHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCEEHHHHHHHHHHCCCCCCCCCCEEEE
FGGGLTPKVPQQWLDGSGADTVLFSDGGGLFDPAHRASTVVEGSAVAACEALTRGLARGV
ECCCCCCCCCHHHCCCCCCCEEEEECCCCCCCCHHHHHHEECCHHHHHHHHHHHHHHHHH
GAWARGFLAAEQRVRTALEAAFAEQPDVLSEPRIAREVVAALPAGAPLFVSSSMPIRDLD
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCHHHHH
AFAPAGTVPLRVLANRGANGIDGIVSSALGMAAAAGRSAVLLTGDLALLHDVGAFVTASR
HHCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCC
TRVPLTVVAVNNDGGGIFSFLPIAQVAPRDTFETLFGTPHGVDLSHAAALGGARLHRPET
CCCEEEEEEEECCCCCEEHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCC
PVALRSAVREGLEGGLHLVEVRVDRNANVDEHRRLFARMAASLGEGPWA
CHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SSDANLNVLWARALLEELVRGGVRHAVVCPGSRSSPLALACAGTEGLRTWSVIDERSAG
CCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEECCCCCEEEHHHHCCCCC
FFALGLAKQSRAPAVVVATSGTAGAHFYPAVIEAALSHVPLVVLTADRPLELQGWGAPQT
EEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCEEEEECCCCEEEECCCCCCC
VPQARFFGEHARFYADLGQPEAHDAALIHMRATVARAVVSAWRAPRGAVQLNVPFREPLA
CCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCC
PIAEPFPEASLSALAKSGRAGAPLTRIVPPEPVPDASTLDEVRRRIAATTRGVIVCGPRD
HHHCCCCHHHHHHHHHCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCEEEECCCH
EMDGFAAAISALSQATGYPVLAESTSQARYGGGPLTLSYYDAMLRHAPFAKAHRPELVLR
HHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCEEHHHHHHHHHHCCCCCCCCCCEEEE
FGGGLTPKVPQQWLDGSGADTVLFSDGGGLFDPAHRASTVVEGSAVAACEALTRGLARGV
ECCCCCCCCCHHHCCCCCCCEEEEECCCCCCCCHHHHHHEECCHHHHHHHHHHHHHHHHH
GAWARGFLAAEQRVRTALEAAFAEQPDVLSEPRIAREVVAALPAGAPLFVSSSMPIRDLD
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCHHHHH
AFAPAGTVPLRVLANRGANGIDGIVSSALGMAAAAGRSAVLLTGDLALLHDVGAFVTASR
HHCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCC
TRVPLTVVAVNNDGGGIFSFLPIAQVAPRDTFETLFGTPHGVDLSHAAALGGARLHRPET
CCCEEEEEEEECCCCCEEHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCC
PVALRSAVREGLEGGLHLVEVRVDRNANVDEHRRLFARMAASLGEGPWA
CHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA