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The map label for this gene is rlmL [H]

Identifier: 108759246

GI number: 108759246

Start: 1716791

End: 1718092

Strand: Reverse

Name: rlmL [H]

Synonym: MXAN_1458

Alternate gene names: 108759246

Gene position: 1718092-1716791 (Counterclockwise)

Preceding gene: 108760885

Following gene: 108763321

Centisome position: 18.8

GC content: 71.74

Gene sequence:

>1302_bases
ATGCCGGTTCCCAAGCGGCCCGGTGTCGTCTATGGGCGGAATCCCATGGCTGAACGCATTGCCCTTTTCGCCACCGCCGC
TCGCGGCACCGAGGACCTCCTGGCCGACGAGCTGAAGGAGCTCGGCGCCCGCCGCATCCGCCAGGACCGCGGCGGTGTCC
GCTTCATGGCCACGCTCGACGAGGCGCTCATGGTGGCGCTCTGGTCCCGCATCGCCATGCGCGTGCTCTACCCGCTGGGC
GCCTTCGAGGCCCGGGGCGCGGAAGGCCTGTACGAAGCCGCCGCCAGCATCCCCTGGGAGGAGCACCTCACCCCCGAACA
CACCTTCGCGGTGGATGCCACGCTGAAGGACAGCGAGCACAGCCACTCCGGCTTCGTGGCCCTCAAGGTGAAAGACGCCA
TCGTCGACCGGATGCGCGACACGAAGGGCGCCCGGCCGGACGTGAATACGAGGGACCCGGACATCCGCGTGGTGGCGCAC
CTCGCCCGTGAAACGCTGTCCCTGTCCCTGGATTTGTGCGGCGAGCCGCTGCACCGCCGGGGCTACCGCGTGCGCCCCAC
GCCCGCCCCGCTGAAGGAGACGCTGGCCGCGGCGGTGCTTCGCGCGGCGAACTACACGGGGACGGAGGGCCTGGTGGACC
CGATGTGCGGCTCCGGCACGTTGCTCATCGAGGCAGGCCTCATCGCCCGCCGGCGCGCGCCCGGGCTCAACCGGGACTTC
GCGGTGGAGCGCTGGCCGGAGCTGGGCACCCGGGCCCGCGAGCTGCTCGCGGACATGCGCGCGGATGCCCGCCGCAACGA
GCGGAAGGTGGAAGTACCGCTGCTCGGCTTCGACAAGGACCCGGAGGCGCTGGAGGCCGCCAGCCGCAACGTGCGCGCCG
CGCGACTGACGGAGGAAATCCAGCTGGCCGAGGGTGACGCGACCCGGCTGCCGCCGCTGCCGGAGACAGGCGGATTGATC
GTCACCAACCCGCCCTACGGCGACCGGCTCGGCACGGGCGGCCAGAAGGGCATGAAGAGCTTCTACTTCAAGCTGGGCGA
GTCGCTGCGCGTGCCGGGCTGGCGCGTGTGGGTCATCTGCGGCAACCCCGCCTTCGAGAGCGCCTTCCACGCGCGCCCCT
CCGCGAAGCGGGACCTCTGGAACGGCCCCATCGCCTGCTCGCTGCTGGGCTACCGTCCTCCAGACGGCGGCAGCGCTCGG
GGTGACACCGGGAACGGCTCAGAAGCGCCGCTCGGTGCGCCGCGCCAGCCGGAGGATGTTGCCCCGGTGCGTCCACAGCA
TGAGGGCGAAGAGGAGGGCTGA

Upstream 100 bases:

>100_bases
CTACTTTCAGTCCGAGGACTTCCAGCGGGCGCGGGAGGCGAGCGCCCAGCAGGAGGACGCCACCTTCGCCCTCTACGAGG
CGGTGGGCACATGAAACGCG

Downstream 100 bases:

>100_bases
GAGTCCCGCGTACTCCACGGCCCGCGCGGTGAGCGCCGACGTGCCCACCGCCGTGGCCCCCGCCGCCAGCGAGCCCAGCG
AGCTCACGCGCGACACGGCG

Product: THUMP domain/methyltransferase domain-containing protein

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MPVPKRPGVVYGRNPMAERIALFATAARGTEDLLADELKELGARRIRQDRGGVRFMATLDEALMVALWSRIAMRVLYPLG
AFEARGAEGLYEAAASIPWEEHLTPEHTFAVDATLKDSEHSHSGFVALKVKDAIVDRMRDTKGARPDVNTRDPDIRVVAH
LARETLSLSLDLCGEPLHRRGYRVRPTPAPLKETLAAAVLRAANYTGTEGLVDPMCGSGTLLIEAGLIARRRAPGLNRDF
AVERWPELGTRARELLADMRADARRNERKVEVPLLGFDKDPEALEAASRNVRAARLTEEIQLAEGDATRLPPLPETGGLI
VTNPPYGDRLGTGGQKGMKSFYFKLGESLRVPGWRVWVICGNPAFESAFHARPSAKRDLWNGPIACSLLGYRPPDGGSAR
GDTGNGSEAPLGAPRQPEDVAPVRPQHEGEEEG

Sequences:

>Translated_433_residues
MPVPKRPGVVYGRNPMAERIALFATAARGTEDLLADELKELGARRIRQDRGGVRFMATLDEALMVALWSRIAMRVLYPLG
AFEARGAEGLYEAAASIPWEEHLTPEHTFAVDATLKDSEHSHSGFVALKVKDAIVDRMRDTKGARPDVNTRDPDIRVVAH
LARETLSLSLDLCGEPLHRRGYRVRPTPAPLKETLAAAVLRAANYTGTEGLVDPMCGSGTLLIEAGLIARRRAPGLNRDF
AVERWPELGTRARELLADMRADARRNERKVEVPLLGFDKDPEALEAASRNVRAARLTEEIQLAEGDATRLPPLPETGGLI
VTNPPYGDRLGTGGQKGMKSFYFKLGESLRVPGWRVWVICGNPAFESAFHARPSAKRDLWNGPIACSLLGYRPPDGGSAR
GDTGNGSEAPLGAPRQPEDVAPVRPQHEGEEEG
>Mature_432_residues
PVPKRPGVVYGRNPMAERIALFATAARGTEDLLADELKELGARRIRQDRGGVRFMATLDEALMVALWSRIAMRVLYPLGA
FEARGAEGLYEAAASIPWEEHLTPEHTFAVDATLKDSEHSHSGFVALKVKDAIVDRMRDTKGARPDVNTRDPDIRVVAHL
ARETLSLSLDLCGEPLHRRGYRVRPTPAPLKETLAAAVLRAANYTGTEGLVDPMCGSGTLLIEAGLIARRRAPGLNRDFA
VERWPELGTRARELLADMRADARRNERKVEVPLLGFDKDPEALEAASRNVRAARLTEEIQLAEGDATRLPPLPETGGLIV
TNPPYGDRLGTGGQKGMKSFYFKLGESLRVPGWRVWVICGNPAFESAFHARPSAKRDLWNGPIACSLLGYRPPDGGSARG
DTGNGSEAPLGAPRQPEDVAPVRPQHEGEEEG

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG0116

COG function: function code L; Predicted N6-adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=377, Percent_Identity=36.604774535809, Blast_Score=199, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 47258; Mature: 47127

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS51165 THUMP ; PS00092 N6_MTASE ; PS01261 UPF0020

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVPKRPGVVYGRNPMAERIALFATAARGTEDLLADELKELGARRIRQDRGGVRFMATLD
CCCCCCCCEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHH
EALMVALWSRIAMRVLYPLGAFEARGAEGLYEAAASIPWEEHLTPEHTFAVDATLKDSEH
HHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHCCCHHHCCCCCCEEEEEEEECCCCC
SHSGFVALKVKDAIVDRMRDTKGARPDVNTRDPDIRVVAHLARETLSLSLDLCGEPLHRR
CCCCEEEEEEHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHC
GYRVRPTPAPLKETLAAAVLRAANYTGTEGLVDPMCGSGTLLIEAGLIARRRAPGLNRDF
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCHHHHHCCCCCCCCC
AVERWPELGTRARELLADMRADARRNERKVEVPLLGFDKDPEALEAASRNVRAARLTEEI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHCCCHHHHHHHHH
QLAEGDATRLPPLPETGGLIVTNPPYGDRLGTGGQKGMKSFYFKLGESLRVPGWRVWVIC
HHCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEE
GNPAFESAFHARPSAKRDLWNGPIACSLLGYRPPDGGSARGDTGNGSEAPLGAPRQPEDV
CCHHHHHHHHCCCCCHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
APVRPQHEGEEEG
CCCCCCCCCCCCC
>Mature Secondary Structure 
PVPKRPGVVYGRNPMAERIALFATAARGTEDLLADELKELGARRIRQDRGGVRFMATLD
CCCCCCCEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHH
EALMVALWSRIAMRVLYPLGAFEARGAEGLYEAAASIPWEEHLTPEHTFAVDATLKDSEH
HHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHCCCHHHCCCCCCEEEEEEEECCCCC
SHSGFVALKVKDAIVDRMRDTKGARPDVNTRDPDIRVVAHLARETLSLSLDLCGEPLHRR
CCCCEEEEEEHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHC
GYRVRPTPAPLKETLAAAVLRAANYTGTEGLVDPMCGSGTLLIEAGLIARRRAPGLNRDF
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCHHHHHCCCCCCCCC
AVERWPELGTRARELLADMRADARRNERKVEVPLLGFDKDPEALEAASRNVRAARLTEEI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHCCCHHHHHHHHH
QLAEGDATRLPPLPETGGLIVTNPPYGDRLGTGGQKGMKSFYFKLGESLRVPGWRVWVIC
HHCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEE
GNPAFESAFHARPSAKRDLWNGPIACSLLGYRPPDGGSARGDTGNGSEAPLGAPRQPEDV
CCHHHHHHHHCCCCCHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
APVRPQHEGEEEG
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA