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The map label for this gene is poxB [H]

Identifier: 108759231

GI number: 108759231

Start: 4361930

End: 4363714

Strand: Direct

Name: poxB [H]

Synonym: MXAN_3654

Alternate gene names: 108759231

Gene position: 4361930-4363714 (Clockwise)

Preceding gene: 108757882

Following gene: 108757575

Centisome position: 47.72

GC content: 67.28

Gene sequence:

>1785_bases
ATGAGCGCCACCGTCGCCGATTATCTGCTCTACCGCCTGACCCAGTGGGGCATCCGCCGGCTCTATGGCTATCCGGGCGA
TGGCATCAACGGCATCATCGGCGCGCTGGGGCGTACCACCGAGCTCCGGTTCATCCAGGCGCGCCACGAGGAGATGGCCG
CCTTCATGGCGTGCGCGCACGCGAAGTTCACGGGAGAGGTGGGCGTTTGTCTGGCTACCTCCGGCCCCGGTGCCATCCAC
CTGCTCAATGGGCTCTACGACGCCAAGCTGGACCACCAGCCCGTGGTGGCCATTGTCGGCCAGCAGGCACGCTCGGCCCT
GGGCGGCCATTACCAGCAGGAGGTGGACCTCACCACGCTCTTCAAGGACGTGGCCAGCGAGTACGTCACCATGGTGACGA
AGACCTCCGCCGTCCGCCACGCCATCGACCGGGCAGTGCGAATCGCGCTGAACCAACGCTCTGTGACGTGCGTCATCATC
CCGAACGACTTGCAGGAGCTGGAGTACCAGAAGCCTCCGCGGAAACACGGCACCGTCCACTCCAGCGTGGACTTCAGCCC
GCCGTACGTCATTCCCCAGGAGAAGGACCTGCGCCGCGCCGCCGCCGTGCTCAATGCCGGCAAGAAGGTGTCCATCCTCA
TCGGCGCGGGTGCGATGAACGCCGCCAACGAGGTGGTGGAGGTGGCCGACCTGTTGGGCGCTGGGGTGGCCAAAGCGCTG
CTGGGCAAGGCGGTGCTGCCGGACGCGCTGCCGTTCGTGACGGGCGCCATCGGCCTGCTGGGGACGAAGCCCTCGTGGGA
CATGATGATGGACTGCGACACGCTGCTGATGGTGGGCACGAGCTTCCCGTACTCGGAGTTGCTCCCACCAGAGGGCCAGG
CGCGCGGCGTGCAGATCGACATCGACGGTCGCATGCTCGCCATCCGCTACCCCATGGAGGTGGCGCTCGCGGGGGACAGC
AAGGAGACGCTGCGCGCGCTGATTCCCATGCTTCAGCGCAAGCAGGACCGGAGCTGGCGCGAGCAGCTCGAGAAGGGCAT
CCGCCAGTGGTGGAAGATGCTGGAGGCGCAGGCGATGAATTCGGCCGACCCCATCAACCCCCAGCGCGTCTTCTGGGAGC
TGAGCCCCAAGCTGCCCGACGGCGTCATTCTGACCGCGGACTCGGGCTCGGGGACGAACTGGTTCGCTCGCGACTTGAAG
ATGCGCAAGGGGATGCTGGCGTCGCTGTCGGGCAACCTCGCCACCATGGGCTGCGGCATGCCGTACGCGATTGGGGCGAA
GTTCGCATTCCCACACCGGCCGGTGCTCGCCATGGTGGGTGACGGCGCGATGCAGATGAACGGCAACGCGGAGCTCGTCA
CCGTGGCGAAGTACTGGAAGGAGTGGAAGGACCCGCGCTTCATCGTCCTGGTGCTCAACAACCGCGACCTCAACCAGGTG
ACGTGGGAGCAGCGTGTCATGGCCGGTGACCCGAAGACGCCGGCCACGCAGACGCTGCCGGACTTCCCCTACGCGCGCTA
CGCGGAGTCGCTGGGCCTGCGCGGCGTGCGTGTGGACCGCCCCGAGCAGATTGCCCGCGCCTGGGACGAGGCGCTCTCCG
CGAACAGGCCCGTGCTGTTGGAGGCCTACACGGACCCCGACGTACCGCCGCTGCCGCCGCACATCACCTTCGAGCAGGCC
CGGAACTTCGCAGCGGCGCTCCTGGAAGGGGACGAGGACGCGCCGGGCGTCATCAAGCAATCCCTCAAGGGCATGGTGGA
CAAGCTCCTCCCGCACAAGGGGTAG

Upstream 100 bases:

>100_bases
AGGGCCCCCGGGCGGGACTGCCGCGTGCCGGTTCCCCGCATCTCCAGGGGATGCCCAGCTTCGAGAGGCACCCCTTCGCT
CCCAGCCCCGAGGAGTTTCA

Downstream 100 bases:

>100_bases
CGGCCGGAAGGGCCGTCGCAACACGTCGCAGGCAGGACCTCTCAGGTTTCATCCGTGGTGAAATGAAACGGGGCCCGACG
CGAAATCACCGCGTCGGGCC

Product: thiamine pyrophosphate protein

Products: Acetate; CO2; Ubiquinol-8 [C]

Alternate protein names: NA

Number of amino acids: Translated: 594; Mature: 593

Protein sequence:

>594_residues
MSATVADYLLYRLTQWGIRRLYGYPGDGINGIIGALGRTTELRFIQARHEEMAAFMACAHAKFTGEVGVCLATSGPGAIH
LLNGLYDAKLDHQPVVAIVGQQARSALGGHYQQEVDLTTLFKDVASEYVTMVTKTSAVRHAIDRAVRIALNQRSVTCVII
PNDLQELEYQKPPRKHGTVHSSVDFSPPYVIPQEKDLRRAAAVLNAGKKVSILIGAGAMNAANEVVEVADLLGAGVAKAL
LGKAVLPDALPFVTGAIGLLGTKPSWDMMMDCDTLLMVGTSFPYSELLPPEGQARGVQIDIDGRMLAIRYPMEVALAGDS
KETLRALIPMLQRKQDRSWREQLEKGIRQWWKMLEAQAMNSADPINPQRVFWELSPKLPDGVILTADSGSGTNWFARDLK
MRKGMLASLSGNLATMGCGMPYAIGAKFAFPHRPVLAMVGDGAMQMNGNAELVTVAKYWKEWKDPRFIVLVLNNRDLNQV
TWEQRVMAGDPKTPATQTLPDFPYARYAESLGLRGVRVDRPEQIARAWDEALSANRPVLLEAYTDPDVPPLPPHITFEQA
RNFAAALLEGDEDAPGVIKQSLKGMVDKLLPHKG

Sequences:

>Translated_594_residues
MSATVADYLLYRLTQWGIRRLYGYPGDGINGIIGALGRTTELRFIQARHEEMAAFMACAHAKFTGEVGVCLATSGPGAIH
LLNGLYDAKLDHQPVVAIVGQQARSALGGHYQQEVDLTTLFKDVASEYVTMVTKTSAVRHAIDRAVRIALNQRSVTCVII
PNDLQELEYQKPPRKHGTVHSSVDFSPPYVIPQEKDLRRAAAVLNAGKKVSILIGAGAMNAANEVVEVADLLGAGVAKAL
LGKAVLPDALPFVTGAIGLLGTKPSWDMMMDCDTLLMVGTSFPYSELLPPEGQARGVQIDIDGRMLAIRYPMEVALAGDS
KETLRALIPMLQRKQDRSWREQLEKGIRQWWKMLEAQAMNSADPINPQRVFWELSPKLPDGVILTADSGSGTNWFARDLK
MRKGMLASLSGNLATMGCGMPYAIGAKFAFPHRPVLAMVGDGAMQMNGNAELVTVAKYWKEWKDPRFIVLVLNNRDLNQV
TWEQRVMAGDPKTPATQTLPDFPYARYAESLGLRGVRVDRPEQIARAWDEALSANRPVLLEAYTDPDVPPLPPHITFEQA
RNFAAALLEGDEDAPGVIKQSLKGMVDKLLPHKG
>Mature_593_residues
SATVADYLLYRLTQWGIRRLYGYPGDGINGIIGALGRTTELRFIQARHEEMAAFMACAHAKFTGEVGVCLATSGPGAIHL
LNGLYDAKLDHQPVVAIVGQQARSALGGHYQQEVDLTTLFKDVASEYVTMVTKTSAVRHAIDRAVRIALNQRSVTCVIIP
NDLQELEYQKPPRKHGTVHSSVDFSPPYVIPQEKDLRRAAAVLNAGKKVSILIGAGAMNAANEVVEVADLLGAGVAKALL
GKAVLPDALPFVTGAIGLLGTKPSWDMMMDCDTLLMVGTSFPYSELLPPEGQARGVQIDIDGRMLAIRYPMEVALAGDSK
ETLRALIPMLQRKQDRSWREQLEKGIRQWWKMLEAQAMNSADPINPQRVFWELSPKLPDGVILTADSGSGTNWFARDLKM
RKGMLASLSGNLATMGCGMPYAIGAKFAFPHRPVLAMVGDGAMQMNGNAELVTVAKYWKEWKDPRFIVLVLNNRDLNQVT
WEQRVMAGDPKTPATQTLPDFPYARYAESLGLRGVRVDRPEQIARAWDEALSANRPVLLEAYTDPDVPPLPPHITFEQAR
NFAAALLEGDEDAPGVIKQSLKGMVDKLLPHKG

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=566, Percent_Identity=24.9116607773852, Blast_Score=128, Evalue=1e-29,
Organism=Escherichia coli, GI1787096, Length=570, Percent_Identity=34.9122807017544, Blast_Score=297, Evalue=2e-81,
Organism=Escherichia coli, GI87081685, Length=561, Percent_Identity=28.698752228164, Blast_Score=196, Evalue=4e-51,
Organism=Escherichia coli, GI1790104, Length=566, Percent_Identity=28.2685512367491, Blast_Score=192, Evalue=7e-50,
Organism=Escherichia coli, GI1786717, Length=545, Percent_Identity=25.8715596330275, Blast_Score=137, Evalue=3e-33,
Organism=Escherichia coli, GI1788716, Length=558, Percent_Identity=22.0430107526882, Blast_Score=83, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17531299, Length=577, Percent_Identity=25.1299826689775, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17531301, Length=577, Percent_Identity=25.1299826689775, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6323755, Length=566, Percent_Identity=27.0318021201413, Blast_Score=163, Evalue=6e-41,
Organism=Saccharomyces cerevisiae, GI6320816, Length=569, Percent_Identity=21.6168717047452, Blast_Score=98, Evalue=4e-21,
Organism=Drosophila melanogaster, GI19922626, Length=572, Percent_Identity=24.4755244755245, Blast_Score=115, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 1.2.2.2 [C]

Molecular weight: Translated: 65083; Mature: 64952

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATVADYLLYRLTQWGIRRLYGYPGDGINGIIGALGRTTELRFIQARHEEMAAFMACAH
CCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
AKFTGEVGVCLATSGPGAIHLLNGLYDAKLDHQPVVAIVGQQARSALGGHYQQEVDLTTL
HCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCEEECCHHHHHHHCCCHHHHHHHHHH
FKDVASEYVTMVTKTSAVRHAIDRAVRIALNQRSVTCVIIPNDLQELEYQKPPRKHGTVH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEEECCCHHHHHCCCCCCCCCCEE
SSVDFSPPYVIPQEKDLRRAAAVLNAGKKVSILIGAGAMNAANEVVEVADLLGAGVAKAL
CCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
LGKAVLPDALPFVTGAIGLLGTKPSWDMMMDCDTLLMVGTSFPYSELLPPEGQARGVQID
HHHHHCCHHHHHHHHHHHHCCCCCCCCHHCCCCEEEEEECCCCHHHCCCCCCCCCCEEEE
IDGRMLAIRYPMEVALAGDSKETLRALIPMLQRKQDRSWREQLEKGIRQWWKMLEAQAMN
ECCEEEEEECCEEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCC
SADPINPQRVFWELSPKLPDGVILTADSGSGTNWFARDLKMRKGMLASLSGNLATMGCGM
CCCCCCHHEEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHCCHHHHCCCCEEEECCCC
PYAIGAKFAFPHRPVLAMVGDGAMQMNGNAELVTVAKYWKEWKDPRFIVLVLNNRDLNQV
CHHHCCEECCCCCCEEEEECCCCEEECCCCCEEEHHHHHHHCCCCEEEEEEECCCCCCHH
TWEQRVMAGDPKTPATQTLPDFPYARYAESLGLRGVRVDRPEQIARAWDEALSANRPVLL
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEEE
EAYTDPDVPPLPPHITFEQARNFAAALLEGDEDAPGVIKQSLKGMVDKLLPHKG
EECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SATVADYLLYRLTQWGIRRLYGYPGDGINGIIGALGRTTELRFIQARHEEMAAFMACAH
CCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
AKFTGEVGVCLATSGPGAIHLLNGLYDAKLDHQPVVAIVGQQARSALGGHYQQEVDLTTL
HCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCEEECCHHHHHHHCCCHHHHHHHHHH
FKDVASEYVTMVTKTSAVRHAIDRAVRIALNQRSVTCVIIPNDLQELEYQKPPRKHGTVH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEEECCCHHHHHCCCCCCCCCCEE
SSVDFSPPYVIPQEKDLRRAAAVLNAGKKVSILIGAGAMNAANEVVEVADLLGAGVAKAL
CCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
LGKAVLPDALPFVTGAIGLLGTKPSWDMMMDCDTLLMVGTSFPYSELLPPEGQARGVQID
HHHHHCCHHHHHHHHHHHHCCCCCCCCHHCCCCEEEEEECCCCHHHCCCCCCCCCCEEEE
IDGRMLAIRYPMEVALAGDSKETLRALIPMLQRKQDRSWREQLEKGIRQWWKMLEAQAMN
ECCEEEEEECCEEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCC
SADPINPQRVFWELSPKLPDGVILTADSGSGTNWFARDLKMRKGMLASLSGNLATMGCGM
CCCCCCHHEEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHCCHHHHCCCCEEEECCCC
PYAIGAKFAFPHRPVLAMVGDGAMQMNGNAELVTVAKYWKEWKDPRFIVLVLNNRDLNQV
CHHHCCEECCCCCCEEEEECCCCEEECCCCCEEEHHHHHHHCCCCEEEEEEECCCCCCHH
TWEQRVMAGDPKTPATQTLPDFPYARYAESLGLRGVRVDRPEQIARAWDEALSANRPVLL
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEEE
EAYTDPDVPPLPPHITFEQARNFAAALLEGDEDAPGVIKQSLKGMVDKLLPHKG
EECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD oxidase,; Lipids site,; Phospholipids; Thiamine diphosphate [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 87000 [C]

Specific activity: 200

Km value (mM): 0.0022 {lysolecithin}} 0.0017 {lecithin}} 0.03 {sodium} 2 {ferricyanide}} 0.0012 {thiamine} 11 {pyruvate}} 10 {pyruvate}} [C]

Substrates: H2O; Pyruvate; Ubiquinone-8 [C]

Specific reaction: H2O + Pyruvate + Ubiquinone-8 --> Acetate + CO2 + Ubiquinol-8 [C]

General reaction: Oxidative decarboxylation; Redox reaction [C]

Inhibitor: 2, 3-Butanedione; Cu2+; Methyl methanethio sulfonate; N-Ethyl maleimide diphosphate; p-Chloromercuribenzoate; Phenyl glyoxal [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]