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The map label for this gene is gluQ [H]

Identifier: 108759102

GI number: 108759102

Start: 1485267

End: 1486214

Strand: Reverse

Name: gluQ [H]

Synonym: MXAN_1271

Alternate gene names: 108759102

Gene position: 1486214-1485267 (Counterclockwise)

Preceding gene: 108759743

Following gene: 108760093

Centisome position: 16.26

GC content: 71.73

Gene sequence:

>948_bases
ATGAGCCAGGTCCGAGGCCGCTTCGCCCCCAGCCCCACCGGGCGCATGCACCTGGGAAACATCCGCAGCGCGCTGCTCGG
CTGGCTCCAGGCGAGGGCGGCCCACGGCCGCTTCCTGCTGCGCATCGAGGACCTGGACCGCGCGCGCTGCAAGCCGCAGT
ACGTGGACGACCTGCTGCGCGACATGGAATGGCTGGGGCTGGACTGGGACGAGCAGCCCGTCTTCCAGAGCCAGCGGGAC
GGCCTCTACCGCGACGCCCAGGCCCGGTTGGAACAAGCAGGGCGCGTGTATCCCTGCTTCTGCACACGCGCGGAGATTGC
ACGGGCCGCCAGCGCGCCTCACGGACTCTCCGACGAAGGCCCCCGCTACCCGGGCACCTGCGCGCACCTCACCCGCGAAC
AGATTTCCGAGCGCGCCCGCACGCGCCCCCCCGCGTACCGCTTCCGCGCGGTCCCCGGCGAGGTCCGCTTCGTGGACGGC
TTGCAGGGCTCGTACTCGCAAGACGTGGACGCGGTGGTGGGCGACTTCGTCGTGCGGCGCAACGACGGCGTGGCCAGCTA
CCAGCTCGCGGTGGTGGTGGACGATGCGGCCACGGACATCACCCACGTGCTGCGCGGGGATGACCTGCTCTCCTCCACGC
CCCGGCAGCTCCAGCTCTACGCCGCGCTCAGCCTGCGCGCGCCCGACTTCCTCCACGTCCCGCTGGTGCTGGGCGAAGAC
GGCAAGCGTCTCGCCAAGCGCGAGGGTGCCTTCGCGCTCGCGGAGTTGCGTGAGCGTGGCGTGGCTCCCGAGCGGGTGCT
GGGCCTGCTCGCCTCATGGAGTGGATGGGGAGACGGTGGCCCCCTGACGCTCGCCGAACTGGTGCACAGCTTCCGTCCCG
AAAGACTGCCGCGCACGCCCATCGTCGCGGGAGAGGCCGCGCTCCGAGAAGCACTCGGCCTGGGGTGA

Upstream 100 bases:

>100_bases
GCACTGCGGCGGGCCCCCCGCGTCGGTTATGGTCCCCCGCGACGATGATTCACTCCGCGTGCCCGCACGCCCTCCTTCCC
CACGGACCTCGGGGGCGCCC

Downstream 100 bases:

>100_bases
GCCCGGGCTCCGCCCTCCTCCACGGCGAGACATCCGGGCGCGGCGGAATCGTGCCTCCGCTGCCCCATGGCCATGTTTGC
CGCGAACACGAGCGCCTCCT

Product: tRNA synthetase class I domain-containing protein

Products: AMP; diphosphate; L-glutamyl-tRNA(Glu)

Alternate protein names: Glu-Q-RSs [H]

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MSQVRGRFAPSPTGRMHLGNIRSALLGWLQARAAHGRFLLRIEDLDRARCKPQYVDDLLRDMEWLGLDWDEQPVFQSQRD
GLYRDAQARLEQAGRVYPCFCTRAEIARAASAPHGLSDEGPRYPGTCAHLTREQISERARTRPPAYRFRAVPGEVRFVDG
LQGSYSQDVDAVVGDFVVRRNDGVASYQLAVVVDDAATDITHVLRGDDLLSSTPRQLQLYAALSLRAPDFLHVPLVLGED
GKRLAKREGAFALAELRERGVAPERVLGLLASWSGWGDGGPLTLAELVHSFRPERLPRTPIVAGEAALREALGLG

Sequences:

>Translated_315_residues
MSQVRGRFAPSPTGRMHLGNIRSALLGWLQARAAHGRFLLRIEDLDRARCKPQYVDDLLRDMEWLGLDWDEQPVFQSQRD
GLYRDAQARLEQAGRVYPCFCTRAEIARAASAPHGLSDEGPRYPGTCAHLTREQISERARTRPPAYRFRAVPGEVRFVDG
LQGSYSQDVDAVVGDFVVRRNDGVASYQLAVVVDDAATDITHVLRGDDLLSSTPRQLQLYAALSLRAPDFLHVPLVLGED
GKRLAKREGAFALAELRERGVAPERVLGLLASWSGWGDGGPLTLAELVHSFRPERLPRTPIVAGEAALREALGLG
>Mature_314_residues
SQVRGRFAPSPTGRMHLGNIRSALLGWLQARAAHGRFLLRIEDLDRARCKPQYVDDLLRDMEWLGLDWDEQPVFQSQRDG
LYRDAQARLEQAGRVYPCFCTRAEIARAASAPHGLSDEGPRYPGTCAHLTREQISERARTRPPAYRFRAVPGEVRFVDGL
QGSYSQDVDAVVGDFVVRRNDGVASYQLAVVVDDAATDITHVLRGDDLLSSTPRQLQLYAALSLRAPDFLHVPLVLGEDG
KRLAKREGAFALAELRERGVAPERVLGLLASWSGWGDGGPLTLAELVHSFRPERLPRTPIVAGEAALREALGLG

Specific function: Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5- dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble positi

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=302, Percent_Identity=37.7483443708609, Blast_Score=189, Evalue=2e-48,
Organism=Escherichia coli, GI1788743, Length=298, Percent_Identity=38.5906040268456, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI226510925, Length=306, Percent_Identity=37.2549019607843, Blast_Score=172, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI71993394, Length=280, Percent_Identity=36.0714285714286, Blast_Score=163, Evalue=9e-41,
Organism=Saccharomyces cerevisiae, GI6324540, Length=284, Percent_Identity=35.9154929577465, Blast_Score=186, Evalue=4e-48,
Organism=Saccharomyces cerevisiae, GI6324742, Length=127, Percent_Identity=30.7086614173228, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24665321, Length=285, Percent_Identity=39.6491228070175, Blast_Score=191, Evalue=5e-49,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR022380
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: 6.1.1.17

Molecular weight: Translated: 34825; Mature: 34694

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQVRGRFAPSPTGRMHLGNIRSALLGWLQARAAHGRFLLRIEDLDRARCKPQYVDDLLR
CCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHCCCHHHHHHHHH
DMEWLGLDWDEQPVFQSQRDGLYRDAQARLEQAGRVYPCFCTRAEIARAASAPHGLSDEG
HHHHCCCCCCCCCHHHHHHCCHHHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCCCCCCCC
PRYPGTCAHLTREQISERARTRPPAYRFRAVPGEVRFVDGLQGSYSQDVDAVVGDFVVRR
CCCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCCEEEECCCCCCCCCHHHHHHHHHHEEC
NDGVASYQLAVVVDDAATDITHVLRGDDLLSSTPRQLQLYAALSLRAPDFLHVPLVLGED
CCCCCEEEEEEEECCHHHHHHHHHCCCHHHHCCCHHEEEEEHHCCCCCCEEEEEEEECCC
GKRLAKREGAFALAELRERGVAPERVLGLLASWSGWGDGGPLTLAELVHSFRPERLPRTP
CHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCC
IVAGEAALREALGLG
CEECHHHHHHHHCCC
>Mature Secondary Structure 
SQVRGRFAPSPTGRMHLGNIRSALLGWLQARAAHGRFLLRIEDLDRARCKPQYVDDLLR
CCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHCCCHHHHHHHHH
DMEWLGLDWDEQPVFQSQRDGLYRDAQARLEQAGRVYPCFCTRAEIARAASAPHGLSDEG
HHHHCCCCCCCCCHHHHHHCCHHHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCCCCCCCC
PRYPGTCAHLTREQISERARTRPPAYRFRAVPGEVRFVDGLQGSYSQDVDAVVGDFVVRR
CCCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCCEEEECCCCCCCCCHHHHHHHHHHEEC
NDGVASYQLAVVVDDAATDITHVLRGDDLLSSTPRQLQLYAALSLRAPDFLHVPLVLGED
CCCCCEEEEEEEECCHHHHHHHHHCCCHHHHCCCHHEEEEEHHCCCCCCEEEEEEEECCC
GKRLAKREGAFALAELRERGVAPERVLGLLASWSGWGDGGPLTLAELVHSFRPERLPRTP
CHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCC
IVAGEAALREALGLG
CEECHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; tRNA(Glu)

Specific reaction: ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA