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The map label for this gene is pleD [H]

Identifier: 108758642

GI number: 108758642

Start: 4451655

End: 4452611

Strand: Reverse

Name: pleD [H]

Synonym: MXAN_3735

Alternate gene names: 108758642

Gene position: 4452611-4451655 (Counterclockwise)

Preceding gene: 108763677

Following gene: 108761440

Centisome position: 48.72

GC content: 68.86

Gene sequence:

>957_bases
ATGGAACGGCGCGGCCGGACCTCCGAGCGGTCCCTTCTGCTCATCATCGAGGATGACGCACAGGTCCGCGAGAGCCTCGT
AGACCTGCTCTCCGTACGCTTCGACGTGCTCGCCGCTCCGGATTCGAATGAGGGGCTGGAGCTGGCGCGGGAGCACCACC
CGGACCTCATCCTCCTGGACAGGTTCCTTCCCAGCGGAGACGGGCTCGCGGTGCTCGAGTTGCTCCAGCGCGAGTCGTGC
ACGGAAGCCGTGCCCGTCATCTTCCTGACGGGCGACGCGGACGAGGCCACGCTGGAGCGCTGCCTGGAGATGGGCGCCGT
GGACTTCATCCACAAGCCGGCCAGCGCGCGGGAGTTGCTGGCCCGTATCGACCGGGCGCTGCGCCAGAGCGAGCAGCAGC
GCCGACTGCAGATCCTCGCGCAGACGGACGGCCTCACGGGGCTCGCCAACTTCCGTGCGCTGAGCATCCGGATGGAAGAA
GAGTTCCGCCGGGCCCAGCGCTACCAGTACCCGCTGAGCGTCGTCATCATCGACCTGGACCACCTCAAGGCCATCAACGA
CGGCATGGGCCATGACGTGGGCAACCGGGCGATTCTCGCGCTGGCCACCCAGTTGAAGCACGAGCTGCGTGAGTCGGACT
TCGCGGCCCGCTTCGGTGGAGACGAGTTCGTCGCGCTGCTGCCGCACCAGACGGCGTTGGAAGCAGCCGTCTTCGCCGAG
CGCATCCGCACCGGGCTGCGCGCCGTCGGCGTCCAGAAGAGCGACGGCCGCCCCGCTCCCTTCGGACTGAGCGTGAGCGT
GGGCATCGCCGACCATACCTTCGAAGGGCCTCGCGAGGACACCGAGTCGCTGATGAAGGCGGCGGACGCGGCCCTCTACG
AGGCAAAGCGCGAGGGGCGGGATCGGGTCGTCGTGTTCGGCCGGCCCGCGAATTCGCCTCCGGCGCAGCGGCACTGA

Upstream 100 bases:

>100_bases
TGCGCGTGGGGCGCAGGGGCGTTGGGAGCACACGGAAGGGCGTGGCACCCGAGACTTTCGGCCTGCCATTTCGCAGCAGC
CGAATGAAGCGGAGTGGTGC

Downstream 100 bases:

>100_bases
ACGGGGCGAGGTGAGGCAGGCAACGGCGGGGCAGCAGCGAGGGCGCGGATGATGCTGGGGAACAGACTGGCGACGGGTTC
GAGGGTGGCGGTGGTGGGAG

Product: response regulator

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MERRGRTSERSLLLIIEDDAQVRESLVDLLSVRFDVLAAPDSNEGLELAREHHPDLILLDRFLPSGDGLAVLELLQRESC
TEAVPVIFLTGDADEATLERCLEMGAVDFIHKPASARELLARIDRALRQSEQQRRLQILAQTDGLTGLANFRALSIRMEE
EFRRAQRYQYPLSVVIIDLDHLKAINDGMGHDVGNRAILALATQLKHELRESDFAARFGGDEFVALLPHQTALEAAVFAE
RIRTGLRAVGVQKSDGRPAPFGLSVSVGIADHTFEGPREDTESLMKAADAALYEAKREGRDRVVVFGRPANSPPAQRH

Sequences:

>Translated_318_residues
MERRGRTSERSLLLIIEDDAQVRESLVDLLSVRFDVLAAPDSNEGLELAREHHPDLILLDRFLPSGDGLAVLELLQRESC
TEAVPVIFLTGDADEATLERCLEMGAVDFIHKPASARELLARIDRALRQSEQQRRLQILAQTDGLTGLANFRALSIRMEE
EFRRAQRYQYPLSVVIIDLDHLKAINDGMGHDVGNRAILALATQLKHELRESDFAARFGGDEFVALLPHQTALEAAVFAE
RIRTGLRAVGVQKSDGRPAPFGLSVSVGIADHTFEGPREDTESLMKAADAALYEAKREGRDRVVVFGRPANSPPAQRH
>Mature_318_residues
MERRGRTSERSLLLIIEDDAQVRESLVDLLSVRFDVLAAPDSNEGLELAREHHPDLILLDRFLPSGDGLAVLELLQRESC
TEAVPVIFLTGDADEATLERCLEMGAVDFIHKPASARELLARIDRALRQSEQQRRLQILAQTDGLTGLANFRALSIRMEE
EFRRAQRYQYPLSVVIIDLDHLKAINDGMGHDVGNRAILALATQLKHELRESDFAARFGGDEFVALLPHQTALEAAVFAE
RIRTGLRAVGVQKSDGRPAPFGLSVSVGIADHTFEGPREDTESLMKAADAALYEAKREGRDRVVVFGRPANSPPAQRH

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1786584, Length=180, Percent_Identity=37.7777777777778, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI145693134, Length=164, Percent_Identity=39.0243902439024, Blast_Score=104, Evalue=9e-24,
Organism=Escherichia coli, GI87082007, Length=173, Percent_Identity=39.8843930635838, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI87081881, Length=183, Percent_Identity=38.7978142076503, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1786599, Length=118, Percent_Identity=40.6779661016949, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787262, Length=201, Percent_Identity=30.3482587064677, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787541, Length=245, Percent_Identity=33.469387755102, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI87081974, Length=162, Percent_Identity=32.7160493827161, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1787802, Length=185, Percent_Identity=27.5675675675676, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1788381, Length=177, Percent_Identity=35.0282485875706, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788394, Length=126, Percent_Identity=35.7142857142857, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787816, Length=169, Percent_Identity=29.585798816568, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI87081977, Length=171, Percent_Identity=27.4853801169591, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1788085, Length=167, Percent_Identity=28.1437125748503, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788192, Length=104, Percent_Identity=37.5, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 35285; Mature: 35285

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERRGRTSERSLLLIIEDDAQVRESLVDLLSVRFDVLAAPDSNEGLELAREHHPDLILLD
CCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHCCCCEEEEE
RFLPSGDGLAVLELLQRESCTEAVPVIFLTGDADEATLERCLEMGAVDFIHKPASARELL
CCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHCCHHHHCCCCHHHHHH
ARIDRALRQSEQQRRLQILAQTDGLTGLANFRALSIRMEEEFRRAQRYQYPLSVVIIDLD
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHHHHHHCCCEEEEEEEHH
HLKAINDGMGHDVGNRAILALATQLKHELRESDFAARFGGDEFVALLPHQTALEAAVFAE
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHH
RIRTGLRAVGVQKSDGRPAPFGLSVSVGIADHTFEGPREDTESLMKAADAALYEAKREGR
HHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
DRVVVFGRPANSPPAQRH
CEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MERRGRTSERSLLLIIEDDAQVRESLVDLLSVRFDVLAAPDSNEGLELAREHHPDLILLD
CCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHCCCCEEEEE
RFLPSGDGLAVLELLQRESCTEAVPVIFLTGDADEATLERCLEMGAVDFIHKPASARELL
CCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHCCHHHHCCCCHHHHHH
ARIDRALRQSEQQRRLQILAQTDGLTGLANFRALSIRMEEEFRRAQRYQYPLSVVIIDLD
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHHHHHHCCCEEEEEEEHH
HLKAINDGMGHDVGNRAILALATQLKHELRESDFAARFGGDEFVALLPHQTALEAAVFAE
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHH
RIRTGLRAVGVQKSDGRPAPFGLSVSVGIADHTFEGPREDTESLMKAADAALYEAKREGR
HHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
DRVVVFGRPANSPPAQRH
CEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA