Click here to switch to the map view.

The map label for this gene is yjeA [H]

Identifier: 108758409

GI number: 108758409

Start: 6930563

End: 6931603

Strand: Reverse

Name: yjeA [H]

Synonym: MXAN_5579

Alternate gene names: 108758409

Gene position: 6931603-6930563 (Counterclockwise)

Preceding gene: 108758798

Following gene: 108763614

Centisome position: 75.84

GC content: 66.76

Gene sequence:

>1041_bases
GTGCATGTGGCTCCTCATGTTACTTGCGCGCTGCCCATGCCCAATCTTTCTCAATGGCGGGCCGCCCGGGGACGCCAGTC
CCTCTACTCCGCCCTGCGACGTTTCTTCGCTGCCCACGGCTACCTGGAGGTGGAGACGCCGCTGCTCATCCCCACTCCGG
GGATGGAGCCGCACATCAACGCGTTCGAGGCCGGCTTCATCCCGGAGACCGATGTGGGCTCCGCGCGGACGCTCTACCTG
CACTCCAGCCCCGAGTACGCCATGAAGCGGCTGCTCGCGGACGGTGCCGGGCCGTTGTTCCAGCTGTGCAAGGTGTTCCG
GAATGGGGAGGTGTCCCCGACCCACAATCCGGAATTCACGATGCTGGAGTTCTACCGGCCTCACGCGGACTACCACGCCA
TCATGGATGACCTGGAAGGGGCGCTGGCGGAGGCCGGCCGCGGCGCGACGGAAGGGGAGCCCGGGGCGGACCCGCTCTTC
TTCACACGCACCCCCTACGAGCGGTTGACGGTGCGTGACGCGGTGCTGCGCGCCACCGGCGTGGACATCCGCCAGCATTC
GGATGGCCCATCATTGAAGCGGGCGGCGGAGGCGGCCGGGGTGCGCACCGGGAACGCGGAGACGTTCGACGACGTCTTCT
TCCACCTCTTCCTCCAGAAGGTGGAGACCGGCTTGGGCCACGAGCGGCCCACCTTCCTCATTGAGTACCCGGCGTCCATG
GCGGCGCTGTCCCGGCTGAAGCCCGGTGACGCGGTGGTGGCGGAGCGGGTGGAGTTGTACGCCAAGGGTTTGGAGCTGGC
GAACGGGTTTTCCGAGCTGACGGACCCCGTGGAACAGCGGGCACGATTGACAGAAGAACAGGAGCTCAGGCGCCAATTGG
GCCGGGCCGTGTATCCTCTGGACGAGCGGTTCCTTGACGCGGTAGGGCGAATGCCACCCTCGGCGGGCATCGCCGTCGGG
CTCGATAGAATCCTGATGCTGCTGCTCGGGGTCCAGCGCATCTCGGACGTGCTCCTTTTCCCCGCCCACGAGTTCGTTTG
A

Upstream 100 bases:

>100_bases
GCTGCTGCTTCTCGGAAAGGACCTGCCGGCCATCGGCGGGCGGGCCCTTGGGGCAGGCAGTGAGGCAGCAGGCGAAGGCC
AGCAGCAGGGGAATCCTTGG

Downstream 100 bases:

>100_bases
TTCGCAAGCTCCTGCAGACGGCCTTTGCTGGAACGGCGGCGGTGGGCATCACCGGTGTGCTGTCTCCCGTGGTGTCGGCG
CTGTCGCTGCGCGACGCCAA

Product: putative lysyl-tRNA synthetase-related protein GenX

Products: AMP; diphosphate; L-lysyl-tRNALys [C]

Alternate protein names: NA

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MHVAPHVTCALPMPNLSQWRAARGRQSLYSALRRFFAAHGYLEVETPLLIPTPGMEPHINAFEAGFIPETDVGSARTLYL
HSSPEYAMKRLLADGAGPLFQLCKVFRNGEVSPTHNPEFTMLEFYRPHADYHAIMDDLEGALAEAGRGATEGEPGADPLF
FTRTPYERLTVRDAVLRATGVDIRQHSDGPSLKRAAEAAGVRTGNAETFDDVFFHLFLQKVETGLGHERPTFLIEYPASM
AALSRLKPGDAVVAERVELYAKGLELANGFSELTDPVEQRARLTEEQELRRQLGRAVYPLDERFLDAVGRMPPSAGIAVG
LDRILMLLLGVQRISDVLLFPAHEFV

Sequences:

>Translated_346_residues
MHVAPHVTCALPMPNLSQWRAARGRQSLYSALRRFFAAHGYLEVETPLLIPTPGMEPHINAFEAGFIPETDVGSARTLYL
HSSPEYAMKRLLADGAGPLFQLCKVFRNGEVSPTHNPEFTMLEFYRPHADYHAIMDDLEGALAEAGRGATEGEPGADPLF
FTRTPYERLTVRDAVLRATGVDIRQHSDGPSLKRAAEAAGVRTGNAETFDDVFFHLFLQKVETGLGHERPTFLIEYPASM
AALSRLKPGDAVVAERVELYAKGLELANGFSELTDPVEQRARLTEEQELRRQLGRAVYPLDERFLDAVGRMPPSAGIAVG
LDRILMLLLGVQRISDVLLFPAHEFV
>Mature_346_residues
MHVAPHVTCALPMPNLSQWRAARGRQSLYSALRRFFAAHGYLEVETPLLIPTPGMEPHINAFEAGFIPETDVGSARTLYL
HSSPEYAMKRLLADGAGPLFQLCKVFRNGEVSPTHNPEFTMLEFYRPHADYHAIMDDLEGALAEAGRGATEGEPGADPLF
FTRTPYERLTVRDAVLRATGVDIRQHSDGPSLKRAAEAAGVRTGNAETFDDVFFHLFLQKVETGLGHERPTFLIEYPASM
AALSRLKPGDAVVAERVELYAKGLELANGFSELTDPVEQRARLTEEQELRRQLGRAVYPLDERFLDAVGRMPPSAGIAVG
LDRILMLLLGVQRISDVLLFPAHEFV

Specific function: Could Be A Lysyl-Trna Synthetase. Mutants In Poxa Have A Reduced Pyruvate Oxidase Activity And A Reduced Growth Rate. [C]

COG id: COG2269

COG function: function code J; Truncated, possibly inactive, lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI194272210, Length=345, Percent_Identity=30.7246376811594, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI5031815, Length=345, Percent_Identity=30.7246376811594, Blast_Score=130, Evalue=2e-30,
Organism=Escherichia coli, GI87082379, Length=332, Percent_Identity=38.2530120481928, Blast_Score=208, Evalue=5e-55,
Organism=Escherichia coli, GI1790571, Length=329, Percent_Identity=31.9148936170213, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1789256, Length=329, Percent_Identity=31.9148936170213, Blast_Score=159, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI71994340, Length=340, Percent_Identity=30, Blast_Score=131, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17535925, Length=340, Percent_Identity=30, Blast_Score=131, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI17535927, Length=340, Percent_Identity=30, Blast_Score=130, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6320242, Length=340, Percent_Identity=28.2352941176471, Blast_Score=124, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24640851, Length=339, Percent_Identity=28.023598820059, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24640849, Length=339, Percent_Identity=28.023598820059, Blast_Score=119, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004525
- InterPro:   IPR018149 [H]

Pfam domain/function: PF00152 tRNA-synt_2 [H]

EC number: 6.1.1.6 [C]

Molecular weight: Translated: 38305; Mature: 38305

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHVAPHVTCALPMPNLSQWRAARGRQSLYSALRRFFAAHGYLEVETPLLIPTPGMEPHIN
CCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCEEEECCCCCCCCC
AFEAGFIPETDVGSARTLYLHSSPEYAMKRLLADGAGPLFQLCKVFRNGEVSPTHNPEFT
HHHCCCCCCCCCCCCEEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCEE
MLEFYRPHADYHAIMDDLEGALAEAGRGATEGEPGADPLFFTRTPYERLTVRDAVLRATG
EEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHC
VDIRQHSDGPSLKRAAEAAGVRTGNAETFDDVFFHLFLQKVETGLGHERPTFLIEYPASM
CCHHCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHH
AALSRLKPGDAVVAERVELYAKGLELANGFSELTDPVEQRARLTEEQELRRQLGRAVYPL
HHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCH
DERFLDAVGRMPPSAGIAVGLDRILMLLLGVQRISDVLLFPAHEFV
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHCC
>Mature Secondary Structure
MHVAPHVTCALPMPNLSQWRAARGRQSLYSALRRFFAAHGYLEVETPLLIPTPGMEPHIN
CCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCEEEECCCCCCCCC
AFEAGFIPETDVGSARTLYLHSSPEYAMKRLLADGAGPLFQLCKVFRNGEVSPTHNPEFT
HHHCCCCCCCCCCCCEEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCEE
MLEFYRPHADYHAIMDDLEGALAEAGRGATEGEPGADPLFFTRTPYERLTVRDAVLRATG
EEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHC
VDIRQHSDGPSLKRAAEAAGVRTGNAETFDDVFFHLFLQKVETGLGHERPTFLIEYPASM
CCHHCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHH
AALSRLKPGDAVVAERVELYAKGLELANGFSELTDPVEQRARLTEEQELRRQLGRAVYPL
HHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCH
DERFLDAVGRMPPSAGIAVGLDRILMLLLGVQRISDVLLFPAHEFV
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 2454 [C]

Specific activity: 1.79

Km value (mM): 0.0236 {L-Lys}} 0.0164 {L-Lys}} 0.0651 {ATP}} 0.0232 {ATP}} [C]

Substrates: ATP; L-lysine; tRNALys [C]

Specific reaction: ATP + L-lysine + tRNALys = AMP + diphosphate + L-lysyl-tRNALys [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 6-Amino-n-hexanoic acid; Adenosine; ATP; Cadaverine; L-Lysineamide; L-Lysinehydroxamate; N-epsilon-Acetyl -L-lysine [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA