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The map label for this gene is pyk [H]

Identifier: 108758117

GI number: 108758117

Start: 4094025

End: 4095425

Strand: Reverse

Name: pyk [H]

Synonym: MXAN_3514

Alternate gene names: 108758117

Gene position: 4095425-4094025 (Counterclockwise)

Preceding gene: 108759250

Following gene: 108758433

Centisome position: 44.81

GC content: 69.24

Gene sequence:

>1401_bases
ATGCGCAAGGCGAAAATCATCTGCACCCTGGGCCCCGCTTCGGACACGCCCGAGGTCATCGAAGGGCTCATCCGCGCGGG
CATGAACATGGCGCGGTTGAACTTCTCGCACGGCACGCACGAGGAGCACCGCCGCCGAGTGGAAACCATCCGCAAGGTCG
CGAAGCGCCTGGGCACGCCGGTGGCCATCCTCCAGGACATCCAGGGGCCGAAGATCCGCCTGGGCCGCTTCGACGGGGGG
CAACTGCTGGTGAAGCAGGGCCAGTCCGTCACGGTGACGACGCGCAACGTCCTGGGCCAGGACGGCGTCATCCCCACCCC
CATCAAGTCGCTGGTGAAGGACGTGGCCAAGGGCCACGAAATCCTCCTGGACGACGGGCGCGTGCGGCTGCGGGTGCTGC
GCGTCCACGGGCAGGACGTGCAGTGCCGCGTGGAGGTGGGCGGCGTGCTCAAGAATCACAAGGGCCTCAACCTGCCCGGC
TCGCCCATGTCGGTGCCCACGATTACGCGCAAGGACGCGGTGGACCTGGCCTTTGGGCAGGAGGTGGGCGTGGACTACGT
GGCGCTGTCCTTCGTCCGCACGCCCGAGGACATCAAGAAGGCGCGCGCGCACGTGGCGAAGCTGAAGACGCCCCTCATCG
CGAAGATTGAAAAGCCCCAGGCGGTGGAGCGCGTGGAAGAGATTGCCGCCGTGGCGGACGGCATCATGGTGGCGCGTGGC
GACCTGGGCGTGGAGATGCCCCTGGAGCGCCTGCCGGCCATCCAGAAGCACATGGTGAGCGCGGTGAACCGCATGGGCGG
GCTCGTCATCGTCGCCACGGAGATGCTGGAGAGCATGGTGGCCAATGCGCGCCCCACCCGCGCCGAGGTGTCCGACGTGG
CCAACGCCATCCTCGACGGCGCGGACGCCGTCATGCTGTCGGGCGAGACGGCCGCGGGCAAGTACCCCGTCGAAGCGGCG
TCCACCATGGCGTGCATCGTGGAGGAGACGGAGCGCGGCGTGGTGAAGCACCAGCACCACTCCCCCTTCGAGCGCTCCGA
GGACCTGGGCACCGGCGTGGCCGCGGCGGCCGTGGCGGCGGCGACGCAACTGGGCATTGGCACCATCGTCGCCTACACGG
AGAGCGGCCACACCGCGCGCCTCATCTCCGAGTTCCGGCCCCACGCGCGCATCATCGCCCTCACGCCGAGCGAGACGGCC
GTGAATCGCATGGCGCTCTACTGGGGCGTGACGGGCATGCAGGTGGCGCGCGTGTCCACCACGGACGCCATGCTCACCCA
GGTGCGTCAGCTGTGCCGGAAGGAGAAGCTCTGCGCGCCCGGCACGCCCGTCGTGGTGGTGGCCGGTGTCCCGCTCAACG
TCCCGGGCAACACCAACCTGATGAGCATCCACCGCATCTGA

Upstream 100 bases:

>100_bases
TCCGCGCGGCCCGAAGCCGGGCCCGCGTGGGTCGAAGAGTTAGTCTCGCGGGCGCAAGGCTGTCAAGCCGGGCACCCCGC
CGCCTAGGATGCGCGGCAAT

Downstream 100 bases:

>100_bases
GCGGGCCCGTGGAGGCCCCGGGACTTGCCGTCACCGGGGCTCCGTGGCCAGCGGGCTCAGAGGACCTTCTGCTCGAAGTC
GTAGATCTTCTCGACGGCGA

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MRKAKIICTLGPASDTPEVIEGLIRAGMNMARLNFSHGTHEEHRRRVETIRKVAKRLGTPVAILQDIQGPKIRLGRFDGG
QLLVKQGQSVTVTTRNVLGQDGVIPTPIKSLVKDVAKGHEILLDDGRVRLRVLRVHGQDVQCRVEVGGVLKNHKGLNLPG
SPMSVPTITRKDAVDLAFGQEVGVDYVALSFVRTPEDIKKARAHVAKLKTPLIAKIEKPQAVERVEEIAAVADGIMVARG
DLGVEMPLERLPAIQKHMVSAVNRMGGLVIVATEMLESMVANARPTRAEVSDVANAILDGADAVMLSGETAAGKYPVEAA
STMACIVEETERGVVKHQHHSPFERSEDLGTGVAAAAVAAATQLGIGTIVAYTESGHTARLISEFRPHARIIALTPSETA
VNRMALYWGVTGMQVARVSTTDAMLTQVRQLCRKEKLCAPGTPVVVVAGVPLNVPGNTNLMSIHRI

Sequences:

>Translated_466_residues
MRKAKIICTLGPASDTPEVIEGLIRAGMNMARLNFSHGTHEEHRRRVETIRKVAKRLGTPVAILQDIQGPKIRLGRFDGG
QLLVKQGQSVTVTTRNVLGQDGVIPTPIKSLVKDVAKGHEILLDDGRVRLRVLRVHGQDVQCRVEVGGVLKNHKGLNLPG
SPMSVPTITRKDAVDLAFGQEVGVDYVALSFVRTPEDIKKARAHVAKLKTPLIAKIEKPQAVERVEEIAAVADGIMVARG
DLGVEMPLERLPAIQKHMVSAVNRMGGLVIVATEMLESMVANARPTRAEVSDVANAILDGADAVMLSGETAAGKYPVEAA
STMACIVEETERGVVKHQHHSPFERSEDLGTGVAAAAVAAATQLGIGTIVAYTESGHTARLISEFRPHARIIALTPSETA
VNRMALYWGVTGMQVARVSTTDAMLTQVRQLCRKEKLCAPGTPVVVVAGVPLNVPGNTNLMSIHRI
>Mature_466_residues
MRKAKIICTLGPASDTPEVIEGLIRAGMNMARLNFSHGTHEEHRRRVETIRKVAKRLGTPVAILQDIQGPKIRLGRFDGG
QLLVKQGQSVTVTTRNVLGQDGVIPTPIKSLVKDVAKGHEILLDDGRVRLRVLRVHGQDVQCRVEVGGVLKNHKGLNLPG
SPMSVPTITRKDAVDLAFGQEVGVDYVALSFVRTPEDIKKARAHVAKLKTPLIAKIEKPQAVERVEEIAAVADGIMVARG
DLGVEMPLERLPAIQKHMVSAVNRMGGLVIVATEMLESMVANARPTRAEVSDVANAILDGADAVMLSGETAAGKYPVEAA
STMACIVEETERGVVKHQHHSPFERSEDLGTGVAAAAVAAATQLGIGTIVAYTESGHTARLISEFRPHARIIALTPSETA
VNRMALYWGVTGMQVARVSTTDAMLTQVRQLCRKEKLCAPGTPVVVVAGVPLNVPGNTNLMSIHRI

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=427, Percent_Identity=39.8126463700234, Blast_Score=298, Evalue=1e-80,
Organism=Homo sapiens, GI33286422, Length=430, Percent_Identity=39.7674418604651, Blast_Score=296, Evalue=2e-80,
Organism=Homo sapiens, GI33286420, Length=430, Percent_Identity=39.7674418604651, Blast_Score=296, Evalue=2e-80,
Organism=Homo sapiens, GI32967597, Length=427, Percent_Identity=41.4519906323185, Blast_Score=293, Evalue=2e-79,
Organism=Homo sapiens, GI10835121, Length=427, Percent_Identity=41.4519906323185, Blast_Score=293, Evalue=2e-79,
Organism=Homo sapiens, GI310128732, Length=287, Percent_Identity=38.3275261324042, Blast_Score=191, Evalue=9e-49,
Organism=Homo sapiens, GI310128730, Length=287, Percent_Identity=38.3275261324042, Blast_Score=191, Evalue=9e-49,
Organism=Homo sapiens, GI310128736, Length=237, Percent_Identity=35.4430379746835, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI310128734, Length=237, Percent_Identity=35.4430379746835, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=34.1121495327103, Blast_Score=125, Evalue=8e-29,
Organism=Escherichia coli, GI1787965, Length=471, Percent_Identity=40.7643312101911, Blast_Score=348, Evalue=4e-97,
Organism=Escherichia coli, GI1788160, Length=475, Percent_Identity=40, Blast_Score=310, Evalue=1e-85,
Organism=Caenorhabditis elegans, GI17544584, Length=479, Percent_Identity=39.6659707724426, Blast_Score=301, Evalue=7e-82,
Organism=Caenorhabditis elegans, GI71984406, Length=424, Percent_Identity=38.6792452830189, Blast_Score=269, Evalue=2e-72,
Organism=Caenorhabditis elegans, GI17506831, Length=424, Percent_Identity=38.6792452830189, Blast_Score=269, Evalue=2e-72,
Organism=Caenorhabditis elegans, GI17506829, Length=424, Percent_Identity=38.6792452830189, Blast_Score=269, Evalue=2e-72,
Organism=Caenorhabditis elegans, GI71984413, Length=424, Percent_Identity=38.6792452830189, Blast_Score=269, Evalue=3e-72,
Organism=Saccharomyces cerevisiae, GI6319279, Length=428, Percent_Identity=37.3831775700935, Blast_Score=266, Evalue=6e-72,
Organism=Saccharomyces cerevisiae, GI6324923, Length=426, Percent_Identity=38.0281690140845, Blast_Score=253, Evalue=4e-68,
Organism=Drosophila melanogaster, GI28571814, Length=473, Percent_Identity=39.7463002114165, Blast_Score=295, Evalue=4e-80,
Organism=Drosophila melanogaster, GI24648964, Length=487, Percent_Identity=39.4250513347023, Blast_Score=295, Evalue=4e-80,
Organism=Drosophila melanogaster, GI24648966, Length=403, Percent_Identity=39.2059553349876, Blast_Score=262, Evalue=5e-70,
Organism=Drosophila melanogaster, GI24581235, Length=469, Percent_Identity=31.7697228144989, Blast_Score=232, Evalue=5e-61,
Organism=Drosophila melanogaster, GI24646914, Length=272, Percent_Identity=37.8676470588235, Blast_Score=159, Evalue=5e-39,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 49990; Mature: 49990

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKAKIICTLGPASDTPEVIEGLIRAGMNMARLNFSHGTHEEHRRRVETIRKVAKRLGTP
CCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCC
VAILQDIQGPKIRLGRFDGGQLLVKQGQSVTVTTRNVLGQDGVIPTPIKSLVKDVAKGHE
CHHHHCCCCCCEEEEECCCCCCEEECCCEEEEEEHHHCCCCCCCCHHHHHHHHHHHCCCE
ILLDDGRVRLRVLRVHGQDVQCRVEVGGVLKNHKGLNLPGSPMSVPTITRKDAVDLAFGQ
EEEECCCEEEEEEEECCCCEEEEEEECCHHCCCCCCCCCCCCCCCCCCCCCCCHHHHCCC
EVGVDYVALSFVRTPEDIKKARAHVAKLKTPLIAKIEKPQAVERVEEIAAVADGIMVARG
CCCCHHEEHHHHCCHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHCCEEEEEC
DLGVEMPLERLPAIQKHMVSAVNRMGGLVIVATEMLESMVANARPTRAEVSDVANAILDG
CCCCCCCHHHCCHHHHHHHHHHHHHCCEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
ADAVMLSGETAAGKYPVEAASTMACIVEETERGVVKHQHHSPFERSEDLGTGVAAAAVAA
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHCCCHHHHHHHHH
ATQLGIGTIVAYTESGHTARLISEFRPHARIIALTPSETAVNRMALYWGVTGMQVARVST
HHHCCCEEEEEEECCCCHHHHHHHCCCCEEEEEECCCHHHHHHHHHEECCCCCEEEEECH
TDAMLTQVRQLCRKEKLCAPGTPVVVVAGVPLNVPGNTNLMSIHRI
HHHHHHHHHHHHHHCCCCCCCCCEEEEECCEEECCCCCCEEEEECC
>Mature Secondary Structure
MRKAKIICTLGPASDTPEVIEGLIRAGMNMARLNFSHGTHEEHRRRVETIRKVAKRLGTP
CCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCC
VAILQDIQGPKIRLGRFDGGQLLVKQGQSVTVTTRNVLGQDGVIPTPIKSLVKDVAKGHE
CHHHHCCCCCCEEEEECCCCCCEEECCCEEEEEEHHHCCCCCCCCHHHHHHHHHHHCCCE
ILLDDGRVRLRVLRVHGQDVQCRVEVGGVLKNHKGLNLPGSPMSVPTITRKDAVDLAFGQ
EEEECCCEEEEEEEECCCCEEEEEEECCHHCCCCCCCCCCCCCCCCCCCCCCCHHHHCCC
EVGVDYVALSFVRTPEDIKKARAHVAKLKTPLIAKIEKPQAVERVEEIAAVADGIMVARG
CCCCHHEEHHHHCCHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHCCEEEEEC
DLGVEMPLERLPAIQKHMVSAVNRMGGLVIVATEMLESMVANARPTRAEVSDVANAILDG
CCCCCCCHHHCCHHHHHHHHHHHHHCCEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
ADAVMLSGETAAGKYPVEAASTMACIVEETERGVVKHQHHSPFERSEDLGTGVAAAAVAA
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHCCCHHHHHHHHH
ATQLGIGTIVAYTESGHTARLISEFRPHARIIALTPSETAVNRMALYWGVTGMQVARVST
HHHCCCEEEEEEECCCCHHHHHHHCCCCEEEEEECCCHHHHHHHHHEECCCCCEEEEECH
TDAMLTQVRQLCRKEKLCAPGTPVVVVAGVPLNVPGNTNLMSIHRI
HHHHHHHHHHHHHHCCCCCCCCCEEEEECCEEECCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11792842; 7559358 [H]