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The map label for this gene is mraW

Identifier: 108757260

GI number: 108757260

Start: 6976813

End: 6977727

Strand: Reverse

Name: mraW

Synonym: MXAN_5612

Alternate gene names: 108757260

Gene position: 6977727-6976813 (Counterclockwise)

Preceding gene: 108758179

Following gene: 108758344

Centisome position: 76.34

GC content: 72.02

Gene sequence:

>915_bases
GTGACGGCTTCGGACTTCCAGCACCAGACCGTCCTCCTGCGGGAAGCAGTGGATCTGCTCCGGCCGGCGGATGGGCGGGT
CATCATTGACGGGACGCTGGGTGGCGGTGGCCACTCGGAGGCGCTGCTGGCCTCGGGCGCCACGGTGGTCGGCGTGGACC
GGGATCCGGTGGCGCTCGCCGCGGCCACCGCGCGCCTGGGCGCGAACCCGCGCTTCCAGGGCCGTGCCGGCAATTTCGCC
GAGCTGCCCCGCGTCGCCGCGGACCTGCTGCCCGTGGATGGCGTGCTGGTGGACCTGGGCGTGTCGTCGCCGCAACTCGA
CGTGGCCGAGCGCGGCTTCTCCTTCTCCAAGGACGGCCCGCTGGACATGCGCATGGGGCCGGACGGCCCGACGGCGGCGG
AGCTGATCGCCACCACGGACGAGCGCGAGCTGGTCCGCATCCTCAAGGACTACGGTGAAGAGCCCTTCGCGCGGCCCATT
GCCCGCGAGCTGAAGAAGGCCCTGCCCACGCGCACGCTGGAGGCCGCGGAGGTCGTGAAGCGGGCGGTGCCGCGCAAGGC
GTGGCCCAACCGCATCCACGTGGCCACCCGGACCTTTCAGGCGCTGCGCATGGCCGTCAACGGCGAACTGGAGGCGCTGG
ACGCGCTGCTGGCCGCCATCCCCGGGCTCCTCAAGGTGGGGGGCCGCGCTGCCGTCATCGCCTTCCATTCCCTGGAGGAC
CGGAAGGTGAAGGAGGCGTTCCGCGCGCTCGCGGGGCGCTGCACCTGTCCGCCGGGCCTGCCCGTGTGCGTATGCAGCGG
GGTGGGTGATTTTGCCCTGGTGACGAAGAAGGCCGTGGCCGCCTCCGAGGCGGAGGTCGAGGCCAATCCCCGTTCACGCA
GCGCGCATCTGCGCGTGGTGGAGAAACTCCGATGA

Upstream 100 bases:

>100_bases
CCGCGCGGCCGGCGCGCACGACTGTTTCGAAATCTACCCGCACATGAACGATGCCCGAGCCGCCTTCGCGCTGGCACGGG
CCCCCTTCGTCTGACGGCCC

Downstream 100 bases:

>100_bases
GCAAGGTGCACTCCCGGGTGTCGGCGTTGCGTTCTTCCGTGACGGTGGGCGGCGTGCTGCTGCACCTGCTGCCGGCCGTG
TGCCTCTTCGCCCTCTTCGT

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MTASDFQHQTVLLREAVDLLRPADGRVIIDGTLGGGGHSEALLASGATVVGVDRDPVALAAATARLGANPRFQGRAGNFA
ELPRVAADLLPVDGVLVDLGVSSPQLDVAERGFSFSKDGPLDMRMGPDGPTAAELIATTDERELVRILKDYGEEPFARPI
ARELKKALPTRTLEAAEVVKRAVPRKAWPNRIHVATRTFQALRMAVNGELEALDALLAAIPGLLKVGGRAAVIAFHSLED
RKVKEAFRALAGRCTCPPGLPVCVCSGVGDFALVTKKAVAASEAEVEANPRSRSAHLRVVEKLR

Sequences:

>Translated_304_residues
MTASDFQHQTVLLREAVDLLRPADGRVIIDGTLGGGGHSEALLASGATVVGVDRDPVALAAATARLGANPRFQGRAGNFA
ELPRVAADLLPVDGVLVDLGVSSPQLDVAERGFSFSKDGPLDMRMGPDGPTAAELIATTDERELVRILKDYGEEPFARPI
ARELKKALPTRTLEAAEVVKRAVPRKAWPNRIHVATRTFQALRMAVNGELEALDALLAAIPGLLKVGGRAAVIAFHSLED
RKVKEAFRALAGRCTCPPGLPVCVCSGVGDFALVTKKAVAASEAEVEANPRSRSAHLRVVEKLR
>Mature_303_residues
TASDFQHQTVLLREAVDLLRPADGRVIIDGTLGGGGHSEALLASGATVVGVDRDPVALAAATARLGANPRFQGRAGNFAE
LPRVAADLLPVDGVLVDLGVSSPQLDVAERGFSFSKDGPLDMRMGPDGPTAAELIATTDERELVRILKDYGEEPFARPIA
RELKKALPTRTLEAAEVVKRAVPRKAWPNRIHVATRTFQALRMAVNGELEALDALLAAIPGLLKVGGRAAVIAFHSLEDR
KVKEAFRALAGRCTCPPGLPVCVCSGVGDFALVTKKAVAASEAEVEANPRSRSAHLRVVEKLR

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=337, Percent_Identity=39.1691394658754, Blast_Score=184, Evalue=7e-47,
Organism=Homo sapiens, GI165377202, Length=188, Percent_Identity=44.1489361702128, Blast_Score=124, Evalue=1e-28,
Organism=Escherichia coli, GI1786270, Length=311, Percent_Identity=45.3376205787781, Blast_Score=213, Evalue=9e-57,
Organism=Drosophila melanogaster, GI62472493, Length=331, Percent_Identity=37.1601208459215, Blast_Score=155, Evalue=2e-38,
Organism=Drosophila melanogaster, GI28571637, Length=331, Percent_Identity=37.1601208459215, Blast_Score=155, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_MYXXD (Q1D0S2)

Other databases:

- EMBL:   CP000113
- RefSeq:   YP_633751.1
- ProteinModelPortal:   Q1D0S2
- SMR:   Q1D0S2
- STRING:   Q1D0S2
- GeneID:   4103436
- GenomeReviews:   CP000113_GR
- KEGG:   mxa:MXAN_5612
- TIGR:   MXAN_5612
- eggNOG:   COG0275
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- ProtClustDB:   PRK00050
- BioCyc:   MXAN246197:MXAN_5612-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 32208; Mature: 32077

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: NA

Important sites: BINDING 53-53 BINDING 79-79 BINDING 98-98 BINDING 105-105

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASDFQHQTVLLREAVDLLRPADGRVIIDGTLGGGGHSEALLASGATVVGVDRDPVALA
CCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHCCCEEEECCCCCHHHH
AATARLGANPRFQGRAGNFAELPRVAADLLPVDGVLVDLGVSSPQLDVAERGFSFSKDGP
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHCCCCCCCCCC
LDMRMGPDGPTAAELIATTDERELVRILKDYGEEPFARPIARELKKALPTRTLEAAEVVK
EEEEECCCCCCHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHH
RAVPRKAWPNRIHVATRTFQALRMAVNGELEALDALLAAIPGLLKVGGRAAVIAFHSLED
HHCCCCCCCCEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCH
RKVKEAFRALAGRCTCPPGLPVCVCSGVGDFALVTKKAVAASEAEVEANPRSRSAHLRVV
HHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
EKLR
HHCC
>Mature Secondary Structure 
TASDFQHQTVLLREAVDLLRPADGRVIIDGTLGGGGHSEALLASGATVVGVDRDPVALA
CCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHCCCEEEECCCCCHHHH
AATARLGANPRFQGRAGNFAELPRVAADLLPVDGVLVDLGVSSPQLDVAERGFSFSKDGP
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHCCCCCCCCCC
LDMRMGPDGPTAAELIATTDERELVRILKDYGEEPFARPIARELKKALPTRTLEAAEVVK
EEEEECCCCCCHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHH
RAVPRKAWPNRIHVATRTFQALRMAVNGELEALDALLAAIPGLLKVGGRAAVIAFHSLED
HHCCCCCCCCEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCH
RKVKEAFRALAGRCTCPPGLPVCVCSGVGDFALVTKKAVAASEAEVEANPRSRSAHLRVV
HHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
EKLR
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA