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The map label for this gene is rlmN2

Identifier: 108757013

GI number: 108757013

Start: 4129525

End: 4130661

Strand: Direct

Name: rlmN2

Synonym: MXAN_3544

Alternate gene names: 108757013

Gene position: 4129525-4130661 (Clockwise)

Preceding gene: 108761188

Following gene: 108759661

Centisome position: 45.18

GC content: 65.96

Gene sequence:

>1137_bases
ATGTCAGAGACCTCCGCCACCTCCCTGCCAGTCACCGAGCCATTGCCGGCTCCCGCGCCCGCCAAGCTGGTGGACGTGGC
CAGCCTGTCGTTGGAGGCGCTGTCGCGCTTCGTCACCGAGGAACTGGGTGAGCGCGCCTTCCGTGCCCCGCAGATCTACC
GCTGGCTGCACCAGCGAGGCGCCACCTCGTTCGACGAGATGACGGACCTCTCCAAGGTCCTGCGTGAGAAGCTCAGGGCG
CGCGCGGAGATCGTCCCCCTGGTGAAGGACTGCGAGCTGCGCAGCACCGACGGCACCATCAAGTACCGGTGGAAGACGCG
GGACGGGCGCTACATCGAATCCGTCTACATGCCCACCGAGGACCGCAGGACGTTGTGCGTGTCCACGCAGGTGGGCTGCG
CCATGGCCTGCGGCTTCTGCATGACGGGCACCATGGGGCTCAAGCGCAACCTGACCCCCAGCGAGATTGTGGCCCAGGTC
CACGCGGTGAACCGCGAGGTTCGCAAGAACGAGGGGCACGAGACGCTCCGCCCGCTCTCCAACCTGGTGTTCATGGGGAT
GGGGGAGCCTCTCCACAACTTCGAGAACCTGAAGACGGCGCTCTCCATCCTCCAGTCCGAGGACGGGCCGAACTTCAGCC
ACCGGCACATCACCGTCTCCACCGTGGGGCTGGTGCCGATGATTGAGCGTTTCGGCAAGGAGACGGACGTCAAGCTGGCC
ATCTCCCTCAACGCCAGCACAGACGAGCAGCGCAGCAAGACGATGCCCGTCAACCGCAAGTGGAACATCGCGGCCTTGCT
GGACGCCTGCCGGAAGTTCCCGCTGCGTCAGGGGCGCCGCATCACCTTCGAGTACGTCCTCATCAAGGGCTTCAACGACG
CGGACGAGGACGCGCACCGGCTCATCGAACTGCTCAAGGGCATCCCGGTGAAGGTGAATCTGATCCCCTACAACGAGAAT
CCCGGCCTGGGGTTCCACACCACCGGCGAGGAGCGGGCGGAGGAATTCCGCGCCATCCTCGCGGACGGCCACGTCGCCGC
CTACATCCGCAGGAACCGGGGCCGGGACATCGCCGGCGCCTGCGGGCAACTCGCCAATCGCGGCGAGACACAAGCCGGGA
CCGATAGTACGACATAA

Upstream 100 bases:

>100_bases
TAGTGTCCACGAGGCGGGCAGGGGAGCATGGGCTCCTTTGACTTGCCGTGCCCAGGTGTGGGAAGGGACGCCCCTGGGCC
CACCGTTAAGCGCAACGACG

Downstream 100 bases:

>100_bases
GTTCCCGAACTTCCTTGACAATCCAACGGGTGCAGCGTTAAGAGCGCCACCCCTTTCCTAAGACGTTTCCTGTTCGTTGG
AGAGCATTCAATGGCCGTTG

Product: radical SAM protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase 2

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MSETSATSLPVTEPLPAPAPAKLVDVASLSLEALSRFVTEELGERAFRAPQIYRWLHQRGATSFDEMTDLSKVLREKLRA
RAEIVPLVKDCELRSTDGTIKYRWKTRDGRYIESVYMPTEDRRTLCVSTQVGCAMACGFCMTGTMGLKRNLTPSEIVAQV
HAVNREVRKNEGHETLRPLSNLVFMGMGEPLHNFENLKTALSILQSEDGPNFSHRHITVSTVGLVPMIERFGKETDVKLA
ISLNASTDEQRSKTMPVNRKWNIAALLDACRKFPLRQGRRITFEYVLIKGFNDADEDAHRLIELLKGIPVKVNLIPYNEN
PGLGFHTTGEERAEEFRAILADGHVAAYIRRNRGRDIAGACGQLANRGETQAGTDSTT

Sequences:

>Translated_378_residues
MSETSATSLPVTEPLPAPAPAKLVDVASLSLEALSRFVTEELGERAFRAPQIYRWLHQRGATSFDEMTDLSKVLREKLRA
RAEIVPLVKDCELRSTDGTIKYRWKTRDGRYIESVYMPTEDRRTLCVSTQVGCAMACGFCMTGTMGLKRNLTPSEIVAQV
HAVNREVRKNEGHETLRPLSNLVFMGMGEPLHNFENLKTALSILQSEDGPNFSHRHITVSTVGLVPMIERFGKETDVKLA
ISLNASTDEQRSKTMPVNRKWNIAALLDACRKFPLRQGRRITFEYVLIKGFNDADEDAHRLIELLKGIPVKVNLIPYNEN
PGLGFHTTGEERAEEFRAILADGHVAAYIRRNRGRDIAGACGQLANRGETQAGTDSTT
>Mature_377_residues
SETSATSLPVTEPLPAPAPAKLVDVASLSLEALSRFVTEELGERAFRAPQIYRWLHQRGATSFDEMTDLSKVLREKLRAR
AEIVPLVKDCELRSTDGTIKYRWKTRDGRYIESVYMPTEDRRTLCVSTQVGCAMACGFCMTGTMGLKRNLTPSEIVAQVH
AVNREVRKNEGHETLRPLSNLVFMGMGEPLHNFENLKTALSILQSEDGPNFSHRHITVSTVGLVPMIERFGKETDVKLAI
SLNASTDEQRSKTMPVNRKWNIAALLDACRKFPLRQGRRITFEYVLIKGFNDADEDAHRLIELLKGIPVKVNLIPYNENP
GLGFHTTGEERAEEFRAILADGHVAAYIRRNRGRDIAGACGQLANRGETQAGTDSTT

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=349, Percent_Identity=43.8395415472779, Blast_Score=281, Evalue=6e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN2_MYXXD (Q1D6I6)

Other databases:

- EMBL:   CP000113
- RefSeq:   YP_631737.1
- ProteinModelPortal:   Q1D6I6
- SMR:   Q1D6I6
- STRING:   Q1D6I6
- GeneID:   4101939
- GenomeReviews:   CP000113_GR
- KEGG:   mxa:MXAN_3544
- TIGR:   MXAN_3544
- eggNOG:   COG0820
- HOGENOM:   HBG335345
- OMA:   CKERITI
- PhylomeDB:   Q1D6I6
- ProtClustDB:   CLSK945767
- BioCyc:   MXAN246197:MXAN_3544-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 42198; Mature: 42067

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETSATSLPVTEPLPAPAPAKLVDVASLSLEALSRFVTEELGERAFRAPQIYRWLHQRG
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCC
ATSFDEMTDLSKVLREKLRARAEIVPLVKDCELRSTDGTIKYRWKTRDGRYIESVYMPTE
CCCHHHHHHHHHHHHHHHHHHHHHHCHHHCCCEECCCCEEEEEEECCCCCEEEEEECCCC
DRRTLCVSTQVGCAMACGFCMTGTMGLKRNLTPSEIVAQVHAVNREVRKNEGHETLRPLS
CCCEEEEECHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHH
NLVFMGMGEPLHNFENLKTALSILQSEDGPNFSHRHITVSTVGLVPMIERFGKETDVKLA
HHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHCCCCCEEEE
ISLNASTDEQRSKTMPVNRKWNIAALLDACRKFPLRQGRRITFEYVLIKGFNDADEDAHR
EEECCCCCHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCHHHHH
LIELLKGIPVKVNLIPYNENPGLGFHTTGEERAEEFRAILADGHVAAYIRRNRGRDIAGA
HHHHHCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHH
CGQLANRGETQAGTDSTT
HHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SETSATSLPVTEPLPAPAPAKLVDVASLSLEALSRFVTEELGERAFRAPQIYRWLHQRG
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCC
ATSFDEMTDLSKVLREKLRARAEIVPLVKDCELRSTDGTIKYRWKTRDGRYIESVYMPTE
CCCHHHHHHHHHHHHHHHHHHHHHHCHHHCCCEECCCCEEEEEEECCCCCEEEEEECCCC
DRRTLCVSTQVGCAMACGFCMTGTMGLKRNLTPSEIVAQVHAVNREVRKNEGHETLRPLS
CCCEEEEECHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHH
NLVFMGMGEPLHNFENLKTALSILQSEDGPNFSHRHITVSTVGLVPMIERFGKETDVKLA
HHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHCCCCCEEEE
ISLNASTDEQRSKTMPVNRKWNIAALLDACRKFPLRQGRRITFEYVLIKGFNDADEDAHR
EEECCCCCHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCHHHHH
LIELLKGIPVKVNLIPYNENPGLGFHTTGEERAEEFRAILADGHVAAYIRRNRGRDIAGA
HHHHHCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHH
CGQLANRGETQAGTDSTT
HHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA