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The map label for this gene is pgk [H]

Identifier: 108563717

GI number: 108563717

Start: 1337030

End: 1338238

Strand: Reverse

Name: pgk [H]

Synonym: HPAG1_1292

Alternate gene names: 108563717

Gene position: 1338238-1337030 (Counterclockwise)

Preceding gene: 108563718

Following gene: 108563716

Centisome position: 83.83

GC content: 41.03

Gene sequence:

>1209_bases
ATGTTAGCTAAAATGTCGTTTATGCAAAATGTTAAAAACATTCAAGAAGTGGAAGTGAGCCATAAAAGGGTGCTTATTAG
AGTGGATTTTAATGTGCCTTTAGATGAAAATTTGAATATTACCGATGACACGCGCATTAGAGAGAGCTTGCCTACCATCC
AATTTTGTATTGACAATAAGGCTAAAGATATTATTTTAGTGAGCCATTTGGGCCGCCCTAAAGGAGTTGAAGAAAAATTA
AGTTTAAAGCCTTTTTTAAAACGCCTTGAAAGACTCTTAAATCATGAAGTGGTTTTTTCTCAAAATATCGCGCAACTCAA
GCAAGCCCTAAACGAAAACGCGCCCACAAGGATTTTTCTTTTAGAAAATATCCGCTTTTTAAAAGGCGAAGAAGAAAATG
ATGAAAATCTGGCTAAAGATTTAGCGAGCTTGTGCGATGTGTTTGTGAATGACGCTTTTGGCACGAGCCACAGAAAGCAC
GCCAGCACTTATGGCACCGCCAAATTCGCCCCTATTAAAGTGAGCGGGTTTTTACTCAAAAAAGAAATTGATTCGTTTTA
TCAAGCGTTTAACCACCCTTTACGCCCTTTATTGTTGATTGTAGGGGGGGCTAAGGTCAGCTCCAAACTCACCCTATTAA
AAAACATTTTAGATCTCATTGACAAGCTCATCATTGCCGGGGCGATGAGTAACACCTTCTTAAAAGCTTTAGGCTATGAT
GTGCAAGATTCTTCTGTAGAAGACGCTTTGATAAAAGACGCCTTAGAGTTGTTAAAAAGCGCGAAAGAAAAAAAAGTCAA
AGTCTATTTGCCTATAGACGCTGTAACCACTGATGACATTCTTAACCCCAAACACATCAAAATTTCACCCGTCCAAGACA
TTGAGCCTAAACACAAGATCGCTGATATAGGGCCTGCGAGCGTGAAATTATTTTCTGAAGTCATAGAGAGCGCGCCCACG
ATCCTATGGAATGGTCCCTTAGGCGTGCATGAAAAACAAGAATTCGCCAGAGGCACAACCTTTTTAGCCCACAAGATCGC
TGACACTTACGCTTTCTCGCTCATTGGTGGGGGCGATACCATTGATGCGATCAACCGCGCGGGCGAAAAGGACAACATGA
GCTTTATTTCTACCGGTGGGGGAGCGAGTTTGGAATTATTAGAGGGCAAAATTTTACCTTGTTTTGAGGTTTTGGACAAA
CGCCATTAA

Upstream 100 bases:

>100_bases
TGCTAAAGTATTGGCATGGTATGATAATGAGATGGGTTATAGCGAGCGCTTGATAGACATGGCGCAGTATATAGCGCAAA
ATTAAAAGAAAGGATTTTTC

Downstream 100 bases:

>100_bases
CTCTAAGGATCGCTTATGGTGAATGTGTTTTTCAAACAGCAAAAATTTGTCATTAAAAAACGCTTTAATGATTTTAATGG
CTTTGACATAGAAGAAAATG

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MLAKMSFMQNVKNIQEVEVSHKRVLIRVDFNVPLDENLNITDDTRIRESLPTIQFCIDNKAKDIILVSHLGRPKGVEEKL
SLKPFLKRLERLLNHEVVFSQNIAQLKQALNENAPTRIFLLENIRFLKGEEENDENLAKDLASLCDVFVNDAFGTSHRKH
ASTYGTAKFAPIKVSGFLLKKEIDSFYQAFNHPLRPLLLIVGGAKVSSKLTLLKNILDLIDKLIIAGAMSNTFLKALGYD
VQDSSVEDALIKDALELLKSAKEKKVKVYLPIDAVTTDDILNPKHIKISPVQDIEPKHKIADIGPASVKLFSEVIESAPT
ILWNGPLGVHEKQEFARGTTFLAHKIADTYAFSLIGGGDTIDAINRAGEKDNMSFISTGGGASLELLEGKILPCFEVLDK
RH

Sequences:

>Translated_402_residues
MLAKMSFMQNVKNIQEVEVSHKRVLIRVDFNVPLDENLNITDDTRIRESLPTIQFCIDNKAKDIILVSHLGRPKGVEEKL
SLKPFLKRLERLLNHEVVFSQNIAQLKQALNENAPTRIFLLENIRFLKGEEENDENLAKDLASLCDVFVNDAFGTSHRKH
ASTYGTAKFAPIKVSGFLLKKEIDSFYQAFNHPLRPLLLIVGGAKVSSKLTLLKNILDLIDKLIIAGAMSNTFLKALGYD
VQDSSVEDALIKDALELLKSAKEKKVKVYLPIDAVTTDDILNPKHIKISPVQDIEPKHKIADIGPASVKLFSEVIESAPT
ILWNGPLGVHEKQEFARGTTFLAHKIADTYAFSLIGGGDTIDAINRAGEKDNMSFISTGGGASLELLEGKILPCFEVLDK
RH
>Mature_402_residues
MLAKMSFMQNVKNIQEVEVSHKRVLIRVDFNVPLDENLNITDDTRIRESLPTIQFCIDNKAKDIILVSHLGRPKGVEEKL
SLKPFLKRLERLLNHEVVFSQNIAQLKQALNENAPTRIFLLENIRFLKGEEENDENLAKDLASLCDVFVNDAFGTSHRKH
ASTYGTAKFAPIKVSGFLLKKEIDSFYQAFNHPLRPLLLIVGGAKVSSKLTLLKNILDLIDKLIIAGAMSNTFLKALGYD
VQDSSVEDALIKDALELLKSAKEKKVKVYLPIDAVTTDDILNPKHIKISPVQDIEPKHKIADIGPASVKLFSEVIESAPT
ILWNGPLGVHEKQEFARGTTFLAHKIADTYAFSLIGGGDTIDAINRAGEKDNMSFISTGGGASLELLEGKILPCFEVLDK
RH

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI31543397, Length=409, Percent_Identity=41.5647921760391, Blast_Score=298, Evalue=9e-81,
Organism=Homo sapiens, GI4505763, Length=415, Percent_Identity=39.7590361445783, Blast_Score=287, Evalue=1e-77,
Organism=Escherichia coli, GI1789294, Length=396, Percent_Identity=42.1717171717172, Blast_Score=303, Evalue=9e-84,
Organism=Caenorhabditis elegans, GI17508823, Length=408, Percent_Identity=40.9313725490196, Blast_Score=292, Evalue=2e-79,
Organism=Saccharomyces cerevisiae, GI6319857, Length=411, Percent_Identity=39.9026763990268, Blast_Score=279, Evalue=5e-76,
Organism=Drosophila melanogaster, GI24581203, Length=410, Percent_Identity=37.5609756097561, Blast_Score=257, Evalue=1e-68,
Organism=Drosophila melanogaster, GI17136394, Length=417, Percent_Identity=38.8489208633094, Blast_Score=252, Evalue=3e-67,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 44700; Mature: 44700

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKMSFMQNVKNIQEVEVSHKRVLIRVDFNVPLDENLNITDDTRIRESLPTIQFCIDNK
CCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHCCEEEEEECCC
AKDIILVSHLGRPKGVEEKLSLKPFLKRLERLLNHEVVFSQNIAQLKQALNENAPTRIFL
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEE
LENIRFLKGEEENDENLAKDLASLCDVFVNDAFGTSHRKHASTYGTAKFAPIKVSGFLLK
ECCCHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCEECEEEEHHHHHH
KEIDSFYQAFNHPLRPLLLIVGGAKVSSKLTLLKNILDLIDKLIIAGAMSNTFLKALGYD
HHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VQDSSVEDALIKDALELLKSAKEKKVKVYLPIDAVTTDDILNPKHIKISPVQDIEPKHKI
CCCCCHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCCCCCCCEEECCCCCCCCCHHH
ADIGPASVKLFSEVIESAPTILWNGPLGVHEKQEFARGTTFLAHKIADTYAFSLIGGGDT
CCCCHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCH
IDAINRAGEKDNMSFISTGGGASLELLEGKILPCFEVLDKRH
HHHHHHCCCCCCCEEEECCCCCEEEECCCCCCHHHHHHCCCC
>Mature Secondary Structure
MLAKMSFMQNVKNIQEVEVSHKRVLIRVDFNVPLDENLNITDDTRIRESLPTIQFCIDNK
CCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHCCEEEEEECCC
AKDIILVSHLGRPKGVEEKLSLKPFLKRLERLLNHEVVFSQNIAQLKQALNENAPTRIFL
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEE
LENIRFLKGEEENDENLAKDLASLCDVFVNDAFGTSHRKHASTYGTAKFAPIKVSGFLLK
ECCCHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCEECEEEEHHHHHH
KEIDSFYQAFNHPLRPLLLIVGGAKVSSKLTLLKNILDLIDKLIIAGAMSNTFLKALGYD
HHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VQDSSVEDALIKDALELLKSAKEKKVKVYLPIDAVTTDDILNPKHIKISPVQDIEPKHKI
CCCCCHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCCCCCCCEEECCCCCCCCCHHH
ADIGPASVKLFSEVIESAPTILWNGPLGVHEKQEFARGTTFLAHKIADTYAFSLIGGGDT
CCCCHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCH
IDAINRAGEKDNMSFISTGGGASLELLEGKILPCFEVLDKRH
HHHHHHCCCCCCCEEEECCCCCEEEECCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]