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The map label for this gene is ngoBIM [H]

Identifier: 108563484

GI number: 108563484

Start: 1117411

End: 1118352

Strand: Reverse

Name: ngoBIM [H]

Synonym: HPAG1_1059

Alternate gene names: 108563484

Gene position: 1118352-1117411 (Counterclockwise)

Preceding gene: 108563485

Following gene: 108563483

Centisome position: 70.06

GC content: 36.2

Gene sequence:

>942_bases
ATGGATTTTTGCTCTGGTATTGGTGGAGGCCGTTTGGGCTTGGAGCGATGCCATTTAAAATGCGTAGGGCATGCCGAAAT
CAATCATGAAGCCCTTAGAACTTATGAATTATTTTTTAAAGATACCCATAATTTTGGGGATTTGATGCGAATCAATCCTA
ATGATTTGCCCGATTTTGATGCACTCATTAGTGGGTTTCCTTGTCAAGCTTTTTCTATCAATGGCAAAAGGAAGGGGCTT
GAAGATGAAAGAGGGACTATTATTTATGGGATTATTCGTATCTTAAAAGTCAAACAGCCCAAATGTTTCTTGCTTGAAAA
CGTTAAGGGCTTGATTAGTCATAAGCAACAAGAAACTTTTAAAACCATTATCAAAGCCTTGCAAGAAGCGGGCTATACAA
CTCATTATCAAATTTTAAACAGCGCTGATTTCCAATTAGCCCAAAAGAGAGAACGCCTTTATATCGTAGGGTTTAGGAAG
GACTTGAAACACCCATTTAATTTCCCTTTAGGTTTAGCCAATGATTATTGTTTTAACGATTTTTTAGACGCTGATAATGA
ATGCTATTTGGATACAAATAACGCTACTTTCCAAAGATACTTGCGCAACCGATACAACCATAACCGGGTTTTTTTAGAGG
ATATTTTAACTTTAGAAAACGCCGTTTTAGACACAAGACAATCTGATTTAAGGTTGTATTTTAATGTTTTTCCTACTTTA
AGGACTTCTCGGCATGGTTTGTTTTATACCCAAAAAGGCAAAATCAAAAAATTAAACGCTATTGAAAGCCTGCTTTTACA
AGGATTTCCTAGAGGTTTGATCGCTAAGATTAAAAATAATCCTAACTTTAAAGAAAGCCATTTATTATCCCAAGCGGGGA
ATGCGATGAGCGTGAATGTGATTGCTGCTATCGCTAAGCAAATGTTAAAGGCGATTCAATAA

Upstream 100 bases:

>100_bases
TTTTAGTTTCTCTGTGTTTTAAAATGCGTTTGTTGCTTTCTTAAATAGGGCATGTTTTTAGTCGTGTGTTAGGAATGATT
GATTGGGGATTTTAACTTTT

Downstream 100 bases:

>100_bases
GGGAGCTTTAAGGGGAGAATGATTTCAAAATACCCCCTATCCCCTTAAGAAATTGGGTTTTACAATAAATAAAATGGCAT
AGTATTTGCTTGAATACGGC

Product: type II DNA modification enzyme

Products: NA

Alternate protein names: M.NgoBI; Cytosine-specific methyltransferase NgoBI; M.NgoI [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MDFCSGIGGGRLGLERCHLKCVGHAEINHEALRTYELFFKDTHNFGDLMRINPNDLPDFDALISGFPCQAFSINGKRKGL
EDERGTIIYGIIRILKVKQPKCFLLENVKGLISHKQQETFKTIIKALQEAGYTTHYQILNSADFQLAQKRERLYIVGFRK
DLKHPFNFPLGLANDYCFNDFLDADNECYLDTNNATFQRYLRNRYNHNRVFLEDILTLENAVLDTRQSDLRLYFNVFPTL
RTSRHGLFYTQKGKIKKLNAIESLLLQGFPRGLIAKIKNNPNFKESHLLSQAGNAMSVNVIAAIAKQMLKAIQ

Sequences:

>Translated_313_residues
MDFCSGIGGGRLGLERCHLKCVGHAEINHEALRTYELFFKDTHNFGDLMRINPNDLPDFDALISGFPCQAFSINGKRKGL
EDERGTIIYGIIRILKVKQPKCFLLENVKGLISHKQQETFKTIIKALQEAGYTTHYQILNSADFQLAQKRERLYIVGFRK
DLKHPFNFPLGLANDYCFNDFLDADNECYLDTNNATFQRYLRNRYNHNRVFLEDILTLENAVLDTRQSDLRLYFNVFPTL
RTSRHGLFYTQKGKIKKLNAIESLLLQGFPRGLIAKIKNNPNFKESHLLSQAGNAMSVNVIAAIAKQMLKAIQ
>Mature_313_residues
MDFCSGIGGGRLGLERCHLKCVGHAEINHEALRTYELFFKDTHNFGDLMRINPNDLPDFDALISGFPCQAFSINGKRKGL
EDERGTIIYGIIRILKVKQPKCFLLENVKGLISHKQQETFKTIIKALQEAGYTTHYQILNSADFQLAQKRERLYIVGFRK
DLKHPFNFPLGLANDYCFNDFLDADNECYLDTNNATFQRYLRNRYNHNRVFLEDILTLENAVLDTRQSDLRLYFNVFPTL
RTSRHGLFYTQKGKIKKLNAIESLLLQGFPRGLIAKIKNNPNFKESHLLSQAGNAMSVNVIAAIAKQMLKAIQ

Specific function: This methylase recognizes the double-stranded sequence RGCGCY, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the NgoBI endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788271, Length=208, Percent_Identity=34.6153846153846, Blast_Score=105, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 35914; Mature: 35914

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: PS00094 C5_MTASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFCSGIGGGRLGLERCHLKCVGHAEINHEALRTYELFFKDTHNFGDLMRINPNDLPDFD
CCCCCCCCCCCCCHHHHHEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHH
ALISGFPCQAFSINGKRKGLEDERGTIIYGIIRILKVKQPKCFLLENVKGLISHKQQETF
HHHCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
KTIIKALQEAGYTTHYQILNSADFQLAQKRERLYIVGFRKDLKHPFNFPLGLANDYCFND
HHHHHHHHHCCCCEEEHHHCCCHHHHHHCCCCEEEEEEHHHCCCCCCCCCCCCCCHHHHH
FLDADNECYLDTNNATFQRYLRNRYNHNRVFLEDILTLENAVLDTRQSDLRLYFNVFPTL
HHCCCCEEEEECCCHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHCCCCCEEEEEEECCCH
RTSRHGLFYTQKGKIKKLNAIESLLLQGFPRGLIAKIKNNPNFKESHLLSQAGNAMSVNV
HCCCCCEEEECCCCCHHHHHHHHHHHHCCCCHHHHEECCCCCHHHHHHHHHCCCCEEHHH
IAAIAKQMLKAIQ
HHHHHHHHHHHCC
>Mature Secondary Structure
MDFCSGIGGGRLGLERCHLKCVGHAEINHEALRTYELFFKDTHNFGDLMRINPNDLPDFD
CCCCCCCCCCCCCHHHHHEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHH
ALISGFPCQAFSINGKRKGLEDERGTIIYGIIRILKVKQPKCFLLENVKGLISHKQQETF
HHHCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
KTIIKALQEAGYTTHYQILNSADFQLAQKRERLYIVGFRKDLKHPFNFPLGLANDYCFND
HHHHHHHHHCCCCEEEHHHCCCHHHHHHCCCCEEEEEEHHHCCCCCCCCCCCCCCHHHHH
FLDADNECYLDTNNATFQRYLRNRYNHNRVFLEDILTLENAVLDTRQSDLRLYFNVFPTL
HHCCCCEEEEECCCHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHCCCCCEEEEEEECCCH
RTSRHGLFYTQKGKIKKLNAIESLLLQGFPRGLIAKIKNNPNFKESHLLSQAGNAMSVNV
HCCCCCEEEECCCCCHHHHHHHHHHHHCCCCHHHHEECCCCCHHHHHHHHHCCCCEEHHH
IAAIAKQMLKAIQ
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9628358 [H]