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The map label for this gene is purB [H]

Identifier: 108563476

GI number: 108563476

Start: 1106510

End: 1107832

Strand: Direct

Name: purB [H]

Synonym: HPAG1_1051

Alternate gene names: 108563476

Gene position: 1106510-1107832 (Clockwise)

Preceding gene: 108563475

Following gene: 108563477

Centisome position: 69.31

GC content: 42.03

Gene sequence:

>1323_bases
GTGTTAGAACGCTATGCGAATGAAGAAATGAAAGCCCTATGGAATGAGCAAACCAAGTTTGAAACCTATTTAGAAGTGGA
AAAAGCTGTCGTTAGGGCGTGGAACAAGCTTGGGCAAATCCAAGATAGCGATTGTGAAAAAATCTGTGCTAAGGCGGCAT
TCAATCTTGAGCGCATCAAAGAAATTGAAAAAACCACTAAGCATGATTTAATCGCTTTCACTACTTGCGTGGCTGAAAGC
CTGGGCGAAGAATCCCGCTTTTTTCATTATGGGATCACTTCTAGCGATTGCATTGATACGGCTATGGCGTTATTGATGAC
AAAAAGCTTAAAACTCATTCAAAAAGGCGTTAAAAACCTCTATGAAACCCTTAAAAACAGGGCTTTAGAGCATAAAGACA
CGCTAATGGTGGGCAGAAGCCATGGGGTGTTTGGCGAACCCATTACTTTTGGCTTAGTGTTAGCCCTTTTTGCTGATGAA
ATCAAACGGCATTTAAAAGCCTTATATTTAACGATGGAATTTATCAGCGTGGGAGCGATCAGTGGGGCTATGGGGAATTT
CGCGCACGCCCCCTTAGAATTAGAAGAATTAGCGTGCGAATTTTTAGGCTTAAAAACCGCCAATATCAGCAATCAAATCA
TTCAAAGAGACCGCTACGCTAGGCTTGCATGCGATCTGGCTCTTTTAGCGAGCAGTTGTGAAAAAATCGCTGTCAATATC
CGCCATTTGCAACGCAGTGAAGTCTATGAAGTGGAAGAAGCTTTTTCAACAGGGCAAAAAGGAAGCTCTGCGATGCCTCA
CAAAAGAAACCCCATATTGAGCGAGAATATCACCGGGCTTTGCAGGGTGATTCGCTCTTTTACGACCCCCATGCTAGAAA
ATGTCGCCTTATGGCATGAAAGGGACATGAGCCATAGCTCTGTGGAGCGTTTTGCATTGCCCGATCTGTTTACCACTAGC
GATTTTATGCTCAGCCGCCTGAATAGCGTGATTGAAAATTTGGTGGTTTATCCTAAAAACATGCTTAAAAATTTAGCTTT
GAGTGGGGGGCTAGTCTTTTCGCAACGGGTGTTATTGGAATTGCCTAAAAAAGGTTTGAGCAGAGAAGAAAGCTATTCTA
TCGTGCAAGAAAATGCGATGAAAGTATGGGAGGTTTTGCAACAAGGCGCTTTTAAAAACGCTGATGAAAATTTATTTTTA
AACGCCCTACTCAACGATGAACGCTTGAAAAAATATTTGAGCGAGAATGAAATCAGAGCATGTTTTGATTACAGCTATTA
CACTAAAAACGTGGGGGCGATTTTTAAAAGGGTGTTTGGATAA

Upstream 100 bases:

>100_bases
TAAATCAAGGCGCTTTTTTGCGTCTTGTGTGATTTTCCCCCTTGACTTTTTAAACCCCTTTAAAGTAACATAAATCCCAT
TTTGATTTTAAGGATTGTCG

Downstream 100 bases:

>100_bases
GGCGCGTTTTTACAAAAACAAAATTATAAAATAAGCGGTAAAAATTGAAGTTTTAAGGGGTTTTTTGAAACGAGCGTTAT
ACTTAATTTTAGGGCTTTTT

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MLERYANEEMKALWNEQTKFETYLEVEKAVVRAWNKLGQIQDSDCEKICAKAAFNLERIKEIEKTTKHDLIAFTTCVAES
LGEESRFFHYGITSSDCIDTAMALLMTKSLKLIQKGVKNLYETLKNRALEHKDTLMVGRSHGVFGEPITFGLVLALFADE
IKRHLKALYLTMEFISVGAISGAMGNFAHAPLELEELACEFLGLKTANISNQIIQRDRYARLACDLALLASSCEKIAVNI
RHLQRSEVYEVEEAFSTGQKGSSAMPHKRNPILSENITGLCRVIRSFTTPMLENVALWHERDMSHSSVERFALPDLFTTS
DFMLSRLNSVIENLVVYPKNMLKNLALSGGLVFSQRVLLELPKKGLSREESYSIVQENAMKVWEVLQQGAFKNADENLFL
NALLNDERLKKYLSENEIRACFDYSYYTKNVGAIFKRVFG

Sequences:

>Translated_440_residues
MLERYANEEMKALWNEQTKFETYLEVEKAVVRAWNKLGQIQDSDCEKICAKAAFNLERIKEIEKTTKHDLIAFTTCVAES
LGEESRFFHYGITSSDCIDTAMALLMTKSLKLIQKGVKNLYETLKNRALEHKDTLMVGRSHGVFGEPITFGLVLALFADE
IKRHLKALYLTMEFISVGAISGAMGNFAHAPLELEELACEFLGLKTANISNQIIQRDRYARLACDLALLASSCEKIAVNI
RHLQRSEVYEVEEAFSTGQKGSSAMPHKRNPILSENITGLCRVIRSFTTPMLENVALWHERDMSHSSVERFALPDLFTTS
DFMLSRLNSVIENLVVYPKNMLKNLALSGGLVFSQRVLLELPKKGLSREESYSIVQENAMKVWEVLQQGAFKNADENLFL
NALLNDERLKKYLSENEIRACFDYSYYTKNVGAIFKRVFG
>Mature_440_residues
MLERYANEEMKALWNEQTKFETYLEVEKAVVRAWNKLGQIQDSDCEKICAKAAFNLERIKEIEKTTKHDLIAFTTCVAES
LGEESRFFHYGITSSDCIDTAMALLMTKSLKLIQKGVKNLYETLKNRALEHKDTLMVGRSHGVFGEPITFGLVLALFADE
IKRHLKALYLTMEFISVGAISGAMGNFAHAPLELEELACEFLGLKTANISNQIIQRDRYARLACDLALLASSCEKIAVNI
RHLQRSEVYEVEEAFSTGQKGSSAMPHKRNPILSENITGLCRVIRSFTTPMLENVALWHERDMSHSSVERFALPDLFTTS
DFMLSRLNSVIENLVVYPKNMLKNLALSGGLVFSQRVLLELPKKGLSREESYSIVQENAMKVWEVLQQGAFKNADENLFL
NALLNDERLKKYLSENEIRACFDYSYYTKNVGAIFKRVFG

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=408, Percent_Identity=26.2254901960784, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI183227688, Length=406, Percent_Identity=27.3399014778325, Blast_Score=122, Evalue=1e-27,
Organism=Escherichia coli, GI1787376, Length=279, Percent_Identity=28.673835125448, Blast_Score=83, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17508577, Length=355, Percent_Identity=26.4788732394366, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI32564234, Length=320, Percent_Identity=27.5, Blast_Score=91, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6323391, Length=386, Percent_Identity=26.4248704663212, Blast_Score=129, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24647570, Length=380, Percent_Identity=26.5789473684211, Blast_Score=118, Evalue=7e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 49964; Mature: 49964

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLERYANEEMKALWNEQTKFETYLEVEKAVVRAWNKLGQIQDSDCEKICAKAAFNLERIK
CCCHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCHHHHH
EIEKTTKHDLIAFTTCVAESLGEESRFFHYGITSSDCIDTAMALLMTKSLKLIQKGVKNL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YETLKNRALEHKDTLMVGRSHGVFGEPITFGLVLALFADEIKRHLKALYLTMEFISVGAI
HHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAMGNFAHAPLELEELACEFLGLKTANISNQIIQRDRYARLACDLALLASSCEKIAVNI
HHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHLQRSEVYEVEEAFSTGQKGSSAMPHKRNPILSENITGLCRVIRSFTTPMLENVALWHE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDMSHSSVERFALPDLFTTSDFMLSRLNSVIENLVVYPKNMLKNLALSGGLVFSQRVLLE
CCCCHHHHHHHCCCCHHCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHH
LPKKGLSREESYSIVQENAMKVWEVLQQGAFKNADENLFLNALLNDERLKKYLSENEIRA
HCHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCHHHHHHHHCCCCHHH
CFDYSYYTKNVGAIFKRVFG
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLERYANEEMKALWNEQTKFETYLEVEKAVVRAWNKLGQIQDSDCEKICAKAAFNLERIK
CCCHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCHHHHH
EIEKTTKHDLIAFTTCVAESLGEESRFFHYGITSSDCIDTAMALLMTKSLKLIQKGVKNL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YETLKNRALEHKDTLMVGRSHGVFGEPITFGLVLALFADEIKRHLKALYLTMEFISVGAI
HHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAMGNFAHAPLELEELACEFLGLKTANISNQIIQRDRYARLACDLALLASSCEKIAVNI
HHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHLQRSEVYEVEEAFSTGQKGSSAMPHKRNPILSENITGLCRVIRSFTTPMLENVALWHE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDMSHSSVERFALPDLFTTSDFMLSRLNSVIENLVVYPKNMLKNLALSGGLVFSQRVLLE
CCCCHHHHHHHCCCCHHCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHH
LPKKGLSREESYSIVQENAMKVWEVLQQGAFKNADENLFLNALLNDERLKKYLSENEIRA
HCHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCHHHHHHHHCCCCHHH
CFDYSYYTKNVGAIFKRVFG
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]