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The map label for this gene is truA [H]

Identifier: 108563457

GI number: 108563457

Start: 1085826

End: 1086554

Strand: Direct

Name: truA [H]

Synonym: HPAG1_1032

Alternate gene names: 108563457

Gene position: 1085826-1086554 (Clockwise)

Preceding gene: 108563456

Following gene: 108563459

Centisome position: 68.02

GC content: 41.56

Gene sequence:

>729_bases
ATGCGTTGTTTTAAGGCTACTATCGCTTATGATGGGGCTTATTTTTTAGGCTATGCCAAACAGCCCAACAAACTCGGCGT
TCAAGATAAAATAGAGAACGCTTTAAATACGCTAGGGATTAAAAGCGTTGTGGTTGCGGCTGGGCGCACGGATAAAGGCG
TGCATGCGAACAATCAGGTTTTGTCCTTTCACGCTCCAAAACACTGGAATGTCGCTAAATTATTTTATTACCTAGCCCCC
AAACTCGCCCCGCATATTGTCTTAAAAAAGCTAGAAGAAAAGAACTTCCATGCGCGTTTTGACGCTCAAAAAAGAGCGTA
TCGTTACCTTTTGACGAAGAATTTAAAAACGCCTTTTTTAGCGCCCTATATCGCTTGTGGGGATTATGGCTCACTAGATT
TATTAAACAGCGCTTTAAAGCAATTCACAGGCAAGCATGATTTTTCTTTGTTTAAGAAAGAAGGCGGGGCTACAACCAAT
CCTAATCGCATCATTTTTAACGCTTTTGCTTATACAGCCTTTATCATGGGGCATGAGTGCGTGGTGTTTAAAATCATTGG
CGATGCGTTTTTACGCTCCAGCGTGCGTTTGATCATTCAAACATGCGTTCAATATTCCTTAGAAAAAATCACGCTCGCTG
AAATTGAAATGCAAATCCACAACATCAAAGCCACCATAAGAACGCCCATAATGGCTGATGGCTTGTATTTGCACAGGGTG
TATTATTGA

Upstream 100 bases:

>100_bases
GATTTCTACGGATTTGTTTTTGATGACCTTTTTCTTCCCCTTTATTTGGGCGTTTGGCTCTTATTTGTTGTTTAGAAAAT
TCATTTTAAAGCGTTATTGA

Downstream 100 bases:

>100_bases
AATCTTAAAAGGTGTTCTTCCCAATCTAGAGCGCTTTTGATAATGGTGTCTAGGTTGTTATAAAGGGGTTTGAAAGGGGT
GTTTTGTAAGATTTTAGCGT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MRCFKATIAYDGAYFLGYAKQPNKLGVQDKIENALNTLGIKSVVVAAGRTDKGVHANNQVLSFHAPKHWNVAKLFYYLAP
KLAPHIVLKKLEEKNFHARFDAQKRAYRYLLTKNLKTPFLAPYIACGDYGSLDLLNSALKQFTGKHDFSLFKKEGGATTN
PNRIIFNAFAYTAFIMGHECVVFKIIGDAFLRSSVRLIIQTCVQYSLEKITLAEIEMQIHNIKATIRTPIMADGLYLHRV
YY

Sequences:

>Translated_242_residues
MRCFKATIAYDGAYFLGYAKQPNKLGVQDKIENALNTLGIKSVVVAAGRTDKGVHANNQVLSFHAPKHWNVAKLFYYLAP
KLAPHIVLKKLEEKNFHARFDAQKRAYRYLLTKNLKTPFLAPYIACGDYGSLDLLNSALKQFTGKHDFSLFKKEGGATTN
PNRIIFNAFAYTAFIMGHECVVFKIIGDAFLRSSVRLIIQTCVQYSLEKITLAEIEMQIHNIKATIRTPIMADGLYLHRV
YY
>Mature_242_residues
MRCFKATIAYDGAYFLGYAKQPNKLGVQDKIENALNTLGIKSVVVAAGRTDKGVHANNQVLSFHAPKHWNVAKLFYYLAP
KLAPHIVLKKLEEKNFHARFDAQKRAYRYLLTKNLKTPFLAPYIACGDYGSLDLLNSALKQFTGKHDFSLFKKEGGATTN
PNRIIFNAFAYTAFIMGHECVVFKIIGDAFLRSSVRLIIQTCVQYSLEKITLAEIEMQIHNIKATIRTPIMADGLYLHRV
YY

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Escherichia coli, GI1788657, Length=243, Percent_Identity=27.5720164609054, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 27416; Mature: 27416

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRCFKATIAYDGAYFLGYAKQPNKLGVQDKIENALNTLGIKSVVVAAGRTDKGVHANNQV
CCEEEEEEEECCEEEEEECCCCCCCCCHHHHHHHHHHCCHHHHEEECCCCCCCCCCCCCE
LSFHAPKHWNVAKLFYYLAPKLAPHIVLKKLEEKNFHARFDAQKRAYRYLLTKNLKTPFL
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCC
APYIACGDYGSLDLLNSALKQFTGKHDFSLFKKEGGATTNPNRIIFNAFAYTAFIMGHEC
CCEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCEEEEHHHHHHHHHHCCCE
VVFKIIGDAFLRSSVRLIIQTCVQYSLEKITLAEIEMQIHNIKATIRTPIMADGLYLHRV
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCEEEEEE
YY
EC
>Mature Secondary Structure
MRCFKATIAYDGAYFLGYAKQPNKLGVQDKIENALNTLGIKSVVVAAGRTDKGVHANNQV
CCEEEEEEEECCEEEEEECCCCCCCCCHHHHHHHHHHCCHHHHEEECCCCCCCCCCCCCE
LSFHAPKHWNVAKLFYYLAPKLAPHIVLKKLEEKNFHARFDAQKRAYRYLLTKNLKTPFL
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCC
APYIACGDYGSLDLLNSALKQFTGKHDFSLFKKEGGATTNPNRIIFNAFAYTAFIMGHEC
CCEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCEEEEHHHHHHHHHHCCCE
VVFKIIGDAFLRSSVRLIIQTCVQYSLEKITLAEIEMQIHNIKATIRTPIMADGLYLHRV
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCEEEEEE
YY
EC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA